LOCUS       sequence-463082-       10461 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression vector for PCSK5(M452I).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10461)
  AUTHORS   Marohl T, Atkins KA, Wang L, Janes KA
  TITLE     PCSK5M452I is a recessive hypomorph exclusive to MCF10DCIS.com 
            cells.
  JOURNAL   Mol Cancer Res. 2025 Oct 28. doi: 10.1158/1541-7786.MCR-25-0211.
  PUBMED    41148168
REFERENCE   2  (bases 1 to 10461)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cancer 
            Res. 2025 Oct 28. doi: 10.1158/1541-7786.MCR-25-0211."
COMMENT     Sequence Label: pLX304 PCSK5(M452I)-V5 blast
FEATURES             Location/Qualifiers
     source          1..10461
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        65..368
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        369..572
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     523..543
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    595..619
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(3359..3383)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             3385..3426
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    3468..4056
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3521..3541)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3939..3950)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             4128..4361
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    4439..4560
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4476..4495)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4530..4549
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      4600..4735
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(4755..4774)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4756..4774)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4784..4801)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4784..4800)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4793..4815)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4942..5397
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5029..5048)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5239..5260
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        5423..5527
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5528..5596,5597..6388)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5746..5765)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6559..7147
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7048..7067
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7301..7318
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7435..7456
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7471..7476,7477..7494,7495..7501)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7509..7525
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7514..7536
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7533..7549
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7533..7549
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     7568..7588
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        7570..7588
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        7616..7842
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             7843..8023
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    8070..8195
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8688..8921
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9106..9150
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        9325..9825
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     9744..9762
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             9837..10232
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     misc_feature    10292..10409
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 cccggggtta ttaatagtaa tcaattacgg ggtcattagt tcatagccca tatatggagt
       61 tccgcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc
      121 cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac
      181 gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata
      241 tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc
      301 agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta
      361 ttaccatggt gatgcggttt tggcagtaca tcaatgggcg tggatagcgg tttgactcac
      421 ggggatttcc aagtctccac cccattgacg tcaatgggag tttgttttgg caccaaaatc
      481 aacgggactt tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc
      541 gtgtacggtg ggaggtctat ataagcagag ctctctggct aactgtcggg atcaacaagt
      601 ttgtacaaaa aagttggcat gggctggggg agccgctgct gctgcccggg acgtttggac
      661 ctgctgtgcg tgctggcgct gctcgggggc tgcctgctcc ccgtgtgtcg gacgcgcgtc
      721 tacaccaacc actgggcagt caaaatcgcc gggggcttcc cggaggccaa ccgtatcgcc
      781 agcaagtacg gattcatcaa cataggacag ataggggccc tgaaggacta ctaccacttc
      841 taccatagca ggacgattaa aaggtcagtt atctcgagca gagggaccca cagtttcatt
      901 tcaatggaac caaaggtgga atggatccaa cagcaagtgg taaaaaagcg gacaaagagg
      961 gattatgact tcagtcgtgc ccagtctacc tatttcaatg atcccaagtg gcccagcatg
     1021 tggtatatgc actgcagtga caatacacat ccctgccagt ctgacatgaa tatcgaagga
     1081 gcctggaaga gaggctacac gggaaagaac attgtggtca ctatcctgga tgacggaatt
     1141 gagagaaccc atccagatct gatgcaaaac tacgatgctc tggcaagttg cgacgtgaat
     1201 gggaatgact tggacccaat gcctcgttat gatgcaagca acgagaacaa gcatgggact
     1261 cgctgtgctg gagaagtggc agccgctgca aacaattcgc actgcacagt cggaattgct
     1321 ttcaacgcca agatcggagg agtgcgaatg ctggacggag atgtcacgga catggttgaa
     1381 gcaaaatcag ttagcttcaa cccccagcac gtgcacattt acagcgccag ctggggcccg
     1441 gatgatgatg gcaagactgt ggacggacca gcccccctca cccggcaagc ctttgaaaac
     1501 ggcgttagaa tggggcggag aggcctcggc tctgtgtttg tttgggcatc tggaaatggt
     1561 ggaaggagca aagaccactg ctcctgtgat ggctacacca acagcatcta caccatctcc
     1621 atcagcagca ctgcagaaag cggaaagaaa ccttggtacc tggaagagtg ttcatccacg
     1681 ctggccacaa cctacagcag cggggagtcc tacgataaga aaatcatcac tacagatctg
     1741 aggcagcgtt gcacggacaa ccacactggg acgtcagcct cagcccccat ggctgcaggc
     1801 atcattgcgc tggccctgga agccaatccg tttctgacct ggagagacgt acagcatgtt
     1861 attgtcagga cttcccgtgc gggacatttg aacgctaatg actggaaaac caatgctgct
     1921 ggttttaagg tgagccatct ttatggattt ggactgatgg acgcagaagc cattgtgatg
     1981 gaggcagaga agtggaccac cgttccccgg cagcacgtgt gtgtggagag cacagaccga
     2041 caaatcaaga caatccgccc taacagtgca gtgcgctcca tctacaaagc ttcaggctgc
     2101 tcggataacc ccaaccgcca tgtcaactac ctggagcacg tcgttgtgcg catcaccatc
     2161 acccacccca ggagaggaga cctggccatc tacctgacct cgccctctgg aactaggtct
     2221 cagcttttgg ccaacaggct atttgatcac tccatggaag gattcaaaaa ctgggagttc
     2281 atgaccattc attgctgggg agaaagagct gctggtgact gggtccttga agtttatgat
     2341 actccctctc agctaaggaa ctttaagact ccaggtaaat tgaaagaatg gtctttggtc
     2401 ctctacggca cctccgtgca gccatattca ccaaccaatg aatttccgaa agtggaacgg
     2461 ttccgctata gccgagttga agaccccaca gacgactatg gcacagagga ttatgcaggt
     2521 ccctgcgacc ctgagtgcag tgaggttggc tgtgacgggc caggaccaga ccactgcaat
     2581 gactgtttgc actactacta caagctgaaa aacaatacca ggatctgtgt ctccagctgc
     2641 ccccctggcc actaccacgc cgacaagaag cgctgcagga agtgtgcccc caactgtgag
     2701 tcctgctttg ggagccatgg tgaccaatgc atgtcctgca aatatggata ctttctgaat
     2761 gaagaaacca acagctgtgt tactcactgc cctgatgggt catatcagga taccaagaaa
     2821 aatctttgcc ggaaatgcag tgaaaactgc aagacatgta ctgaattcca taactgtaca
     2881 gaatgtaggg atgggttaag cctgcaggga tcccggtgct ctgtctcctg tgaagatgga
     2941 cggtatttca acggccagga ctgccagccc tgccaccgct tctgcgccac ttgtgctggg
     3001 gcaggagctg atgggtgcat taactgcaca gagggctact tcatggagga tgggagatgc
     3061 gtgcagagct gtagtatcag ctattacttt gaccactctt cagagaatgg atacaaatcc
     3121 tgcaaaaaat gtgatatcag ttgtttgacg tgcaatggcc caggattcaa gaactgtaca
     3181 agctgcccta gtgggtatct cttagactta ggaatgtgtc aaatgggagc catttgcaag
     3241 gatgcaacgg aagagtcctg ggcggaagga ggcttctgta tgcttgtgaa aaagaacaat
     3301 ctgtgccaac ggaaggttct tcaacaactt tgctgcaaaa catgtacatt tcaaggctgc
     3361 ccaactttct tgtacaaagt ggttggtaag cctatcccta accctctcct cggtctcgat
     3421 tctacgtagt aatgagctag cgctaaccgg tggcgcgtta agtcgacaat caacctctgg
     3481 attacaaaat ttgtgaaaga ttgactggta ttcttaacta tgttgctcct tttacgctat
     3541 gtggatacgc tgctttaatg cctttgtatc atgctattgc ttcccgtatg gctttcattt
     3601 tctcctcctt gtataaatcc tggttgctgt ctctttatga ggagttgtgg cccgttgtca
     3661 ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt tggggcattg
     3721 ccaccacctg tcagctcctt tccgggactt tcgctttccc cctccctatt gccacggcgg
     3781 aactcatcgc cgcctgcctt gcccgctgct ggacaggggc tcggctgttg ggcactgaca
     3841 attccgtggt gttgtcgggg aaatcatcgt cctttccttg gctgctcgcc tgtgttgcca
     3901 cctggattct gcgcgggacg tccttctgct acgtcccttc ggccctcaat ccagcggacc
     3961 ttccttcccg cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc
     4021 agacgagtcg gatctccctt tgggccgcct ccccgcgtcg actttaagac caatgactta
     4081 caaggcagct gtagatctta gccacttttt aaaagaaaag gggggactgg aagggctaat
     4141 tcactcccaa cgaagacaag atctgctttt tgcttgtact gggtctctct ggttagacca
     4201 gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag
     4261 cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag
     4321 atccctcaga cccttttagt cagtgtggaa aatctctagc agtacgtata gtagttcatg
     4381 tcatcttatt attcagtatt tataacttgc aaagaaatga atatcagaga gtgagaggaa
     4441 cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa
     4501 taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta
     4561 tcatgtctgg ctctagctat cccgccccta actccgccca tcccgcccct aactccgccc
     4621 agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag
     4681 gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggg
     4741 acgtacccaa ttcgccctat agtgagtcgt attacgcgcg ctcactggcc gtcgttttac
     4801 aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa tcgccttgca gcacatcccc
     4861 ctttcgccag ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc caacagttgc
     4921 gcagcctgaa tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg
     4981 tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt
     5041 tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc
     5101 tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg
     5161 gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg
     5221 agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct
     5281 cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg
     5341 agctgattta acaaaaattt aacgcgaatt ttaacaaaat attaacgctt acaatttagg
     5401 tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc
     5461 aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag
     5521 gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg
     5581 ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt
     5641 gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt
     5701 tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt
     5761 attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa
     5821 tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag
     5881 agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac
     5941 aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac
     6001 tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac
     6061 cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac
     6121 tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact
     6181 tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg
     6241 tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt
     6301 tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat
     6361 aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta
     6421 gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa
     6481 tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga
     6541 aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac
     6601 aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt
     6661 tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc
     6721 gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat
     6781 cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag
     6841 acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc
     6901 cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag
     6961 cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac
     7021 aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg
     7081 gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggctgagcct
     7141 atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc
     7201 tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga
     7261 gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga
     7321 agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg
     7381 cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatgt
     7441 gagttagctc actcattagg caccccaggc tttacacttt atgcttccgg ctcgtatgtt
     7501 gtgtggaatt gtgagcggat aacaatttca cacaggaaac agctatgacc atgattacgc
     7561 caagcgcgca attaaccctc actaaaggga acaaaagctg gagctgcaag cttaatgtag
     7621 tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca acatgcctta
     7681 caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta cgatcgtgcc
     7741 ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga attgccgcat
     7801 tgcagagata ttgtatttaa gtgcctagct cgatacataa acgggtctct ctggttagac
     7861 cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa
     7921 agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag
     7981 agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc ccgaacaggg
     8041 acttgaaagc gaaagggaaa ccagaggagc tctctcgacg caggactcgg cttgctgaag
     8101 cgcgcacggc aagaggcgag gggcggcgac tggtgagtac gccaaaaatt ttgactagcg
     8161 gaggctagaa ggagagagat gggtgcgaga gcgtcagtat taagcggggg agaattagat
     8221 cgcgatggga aaaaattcgg ttaaggccag ggggaaagaa aaaatataaa ttaaaacata
     8281 tagtatgggc aagcagggag ctagaacgat tcgcagttaa tcctggcctg ttagaaacat
     8341 cagaaggctg tagacaaata ctgggacagc tacaaccatc ccttcagaca ggatcagaag
     8401 aacttagatc attatataat acagtagcaa ccctctattg tgtgcatcaa aggatagaga
     8461 taaaagacac caaggaagct ttagacaaga tagaggaaga gcaaaacaaa agtaagacca
     8521 ccgcacagca agcggccgct gatcttcaga cctggaggag gagatatgag ggacaattgg
     8581 agaagtgaat tatataaata taaagtagta aaaattgaac cattaggagt agcacccacc
     8641 aaggcaaaga gaagagtggt gcagagagaa aaaagagcag tgggaatagg agctttgttc
     8701 cttgggttct tgggagcagc aggaagcact atgggcgcag cgtcaatgac gctgacggta
     8761 caggccagac aattattgtc tggtatagtg cagcagcaga acaatttgct gagggctatt
     8821 gaggcgcaac agcatctgtt gcaactcaca gtctggggca tcaagcagct ccaggcaaga
     8881 atcctggctg tggaaagata cctaaaggat caacagctcc tggggatttg gggttgctct
     8941 ggaaaactca tttgcaccac tgctgtgcct tggaatgcta gttggagtaa taaatctctg
     9001 gaacagattt ggaatcacac gacctggatg gagtgggaca gagaaattaa caattacaca
     9061 agcttaatac actccttaat tgaagaatcg caaaaccagc aagaaaagaa tgaacaagaa
     9121 ttattggaat tagataaatg ggcaagtttg tggaattggt ttaacataac aaattggctg
     9181 tggtatataa aattattcat aatgatagta ggaggcttgg taggtttaag aatagttttt
     9241 gctgtacttt ctatagtgaa tagagttagg cagggatatt caccattatc gtttcagacc
     9301 cacctcccaa ccccgagggg acccttgcgc cttttccaag gcagccctgg gtttgcgcag
     9361 ggacgcggct gctctgggcg tggttccggg aaacgcagcg gcgccgaccc tgggtctcgc
     9421 acattcttca cgtccgttcg cagcgtcacc cggatcttcg ccgctaccct tgtgggcccc
     9481 ccggcgacgc ttcctgctcc gcccctaagt cgggaaggtt ccttgcggtt cgcggcgtgc
     9541 cggacgtgac aaacggaagc cgcacgtctc actagtaccc tcgcagacgg acagcgccag
     9601 ggagcaatgg cagcgcgccg accgcgatgg gctgtggcca atagcggctg ctcagcaggg
     9661 cgcgccgaga gcagcggccg ggaaggggcg gtgcgggagg cggggtgtgg ggcggtagtg
     9721 tgggccctgt tcctgcccgc gcggtgttcc gcattctgca agcctccgga gcgcacgtcg
     9781 gcagtcggct ccctcgttga ccgaatcacc gacctctctc cccagggggt accaccatgg
     9841 ccaagccttt gtctcaagaa gaatccaccc tcattgaaag agcaacggct acaatcaaca
     9901 gcatccccat ctctgaagac tacagcgtcg ccagcgcagc tctctctagc gacggccgca
     9961 tcttcactgg tgtcaatgta tatcatttta ctgggggacc ttgtgcagaa ctcgtggtgc
    10021 tgggcactgc tgctgctgcg gcagctggca acctgacttg tatcgtcgcg atcggaaatg
    10081 agaacagggg catcttgagc ccctgcggac ggtgccgaca ggtgcttctc gatctgcatc
    10141 ctgggatcaa agccatagtg aaggacagtg atggacagcc gacggcagtt gggattcgtg
    10201 aattgctgcc ctctggttat gtgtgggagg gcctgcagct gcagtagtaa gaattctaga
    10261 tcttgagaca aatggcagta ttcatccaca attttaaaag aaaagggggg attggggggt
    10321 acagtgcagg ggaaagaata gtagacataa tagcaacaga catacaaact aaagaattac
    10381 aaaaacaaat tacaaaaatt caaaattttc gggtttatta cagggacagc agagatccac
    10441 tttggcgccg gctcgagggg g
//
