LOCUS       sequence-46311-e        8495 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8495)
  AUTHORS   Pratt ZL, Zhang J, Sugden B
  TITLE     The Latent Membrane Protein 1 (LMP1) Oncogene of Epstein-Barr Virus 
            Can Simultaneously Induce and Inhibit Apoptosis in B Cells.
  JOURNAL   J Virol. 2012 Apr;86(8):4380-93. Epub 2012 Feb 8.
  PUBMED    22318153
REFERENCE   2  (bases 1 to 8495)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Virol."; 
            date: "2012-04"; volume: "86(8)"; pages: "4380-93. Epub 2012 Feb 8"
COMMENT     Sequence Label: pCMMP-LMP1-IRES-eGFP
FEATURES             Location/Qualifiers
     source          1..8495
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        84..387
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        388..591
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     542..562
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     misc_feature    813..1170
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             1235..1651
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1581..1603
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1619..1635
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     regulatory      2912..2921
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    3311..3876
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    3327..3878
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(3471..3488)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     3698..3717
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(3890..3892,3893..3895,3896..4609)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3935..3956)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4196..4215)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4543..4564
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(5876..5892)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5876..5892)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5889..5911)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5900..5916
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5924..5930,5931..5948,5949..5954))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5969..5990
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6107..6124)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6278..6866)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6358..6377)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7037..7828,7829..7897))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7660..7679
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7898..8002)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     8070..8088
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(8126..8148)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     8248..8267
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8461..8483
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     8475..8492
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     8476..8492
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 aagcttgcat gcctgcaggt cgactagtta ttaatagtaa tcaattacgg ggtcattagt
       61 tcatagccca tattggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga
      121 ccgcccaacg acccccgccc cttgacgtca ataatgacgt atgttcccat agtaacgcca
      181 atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca
      241 gtacatcaag tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg
      301 cccgcctggc attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc
      361 tacgtattag tcatcgctat taccatggtg atgcggttgt ggcagtacat caatgggcgt
      421 ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt
      481 ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg
      541 acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc tcgtttagtg
      601 aaccggcgcc agtcctccga tagactgcgt cgcccgggta cccgtgttct caataaaccc
      661 tcttgcagtt gcatccgact cgtggtctcg ctgttccttg ggagggtctc ctctgagtga
      721 ttgactaccc gtcagcgggg tctttcattt ggaggttcca ccgagatttg gagacccctg
      781 cccagggacc accgaccccc ccgccgggag gtaagctggc cagcaactta tctgtgtctg
      841 tccgattgtc tagtgtctat gactgatttt atgcgcctgc gtcggtacta gttagctaac
      901 tagctctgta tctggcggac ccgtggtgga actgacgagt tcggaacacc cggccgcaac
      961 cctgggagac gtcccaggga cttcgggggc cgtttttgtg gcccgacctg agtcctaaaa
     1021 tcccgatcgt ttaggactct ttggtgcacc ccccttagag gagggatatg tggttctggt
     1081 aggagacgag aacctaaaac agttcccgcc tccgtctgaa tttttgcttt cggtttggga
     1141 ccgaagccgc gccgcgcgtc ttgtctgctg cagcatcgtt ctgtgttgtc tctgtctgac
     1201 tgtgtttctg tatttgtctg aaaatatggg cccgggctag actgttacca ctcccttaag
     1261 tttgacctta ggtcactgga aagatgtcga gcggatcgct cacaaccagt cggtagatgt
     1321 caagaagaga cgttgggtta ccttctgctc tgcagaatgg ccaaccttta acgtcggatg
     1381 gccgcgagac ggcaccttta accgagacct catcacccag gttaagatca aggtcttttc
     1441 acctggcccg catggacacc cagaccaggt cccctacatc gtgacctggg aagccttggc
     1501 ttttgacccc cctccctggg tcaagccctt tgtacaccct aagcctccgc ctcctcttcc
     1561 tccatccgcc ccgtctctcc cccttgaacc tcctcgttcg accccgcctc gatcctccct
     1621 ttatccagcc ctcactcctt ctctaggcgc ccccatatgg ccatatgaga tcttatatgg
     1681 ggcacccccg ccccttgtaa acttccctga ccctgacatg acaagagtta ctaacagccc
     1741 ctctctccaa gctcacttac aggctctcta cttagtccag cacgaagtct ggagacctct
     1801 ggcggcagcc taccaagaac aactggaccg accggtcaat tcccggggat ccgttgagga
     1861 tggaacacga ccttgagagg ggcccaccgg gcccgcgacg gccccctcga ggaccccccc
     1921 tctcctcttc cctaggcctt gctctccttc tcctcctctt ggcgctactg ttttggctgt
     1981 acatcgttat gagtgactgg actggaggag ccctccttgt cctctattcc tttgctctca
     2041 tgcttataat tataattttg atcatcttta tcttcagaag agaccttctc tgtccacttg
     2101 gagccctttg tatactccta ctgatgatca ccctcctgct catcgctctc tggaatttgc
     2161 acggacaggc attgtacctt ggaattgtgc tgttcatctt cgggtgctta cttgtcttag
     2221 gtctctggat ctacttattg gagattctct ggcgacttgg tgccaccatc tggcagcttt
     2281 tggccttctt cctagccttc ttcctagacc tcatcctgct cattattgct ctctatctac
     2341 aacaaaactg gtggactcta ttggttgatc tcctttggct cctcctgttt ctggcgattt
     2401 taatctggat gtattaccat ggacaacgac acagtgatga acaccaccac gatgactccc
     2461 tcccgcaccc tcaacaagct accgatgatt ctggccatga atctgactct aactccaacg
     2521 agggcagaca ccacctgctc gtgagtggag ccggcgacgg acccccactc tgctctcaaa
     2581 acctaggcgc acctggaggt ggtcctgaca atggcccaca ggaccctgac aacactgatg
     2641 acaatggccc acaggaccct gacaacactg atgacaatgg cccacatgac ccgctgcctc
     2701 aggaccctga caacactgat gacaatggcc cacaggaccc tgacaacact gatgacaatg
     2761 gcccacatga cccgctgcct catagcccta gcgactctgc tggaaatgat ggaggccctc
     2821 cacaattgac ggaagaggtt gaaaacaaag gaggtgacca gggcccgcct ttgatgacag
     2881 acggaggcgg cggtcatagt catgattccg gccatggcgg cggtgatcca caccttccta
     2941 cgctgctttt gggttcttct ggttccggtg gagatgatga cgacccccac ggcccagttc
     3001 agctaagcta ctatgactaa cctttcttta cttctaggca ttaccatgtc ataggcttgc
     3061 ctgactgact ctccctccat ttactgggaa tgccttagct aatcacctta actggcacac
     3121 actcccttag ccacactgtc tgtctaggct gaaaagccac attcatattc tatttcaaaa
     3181 caaggggaaa ggaggacatg cgagaattgg cagacacctt tacccagccc ttaacacacc
     3241 acacaggtag caaggacccg ggtctggcca ctcgagacgc gtatgcatgt atacctcgag
     3301 gatccggaat tccgcccccc cccctaacgt tactggccga agccgcttgg aataaggccg
     3361 gtgtgcgttt gtctatatgt tattttccac catattgccg tcttttggca atgtgagggc
     3421 ccggaaacct ggccctgtct tcttgacgag cattcctagg ggtctttccc ctctcgccaa
     3481 aggaatgcaa ggtctgttga atgtcgtgaa ggaagcagtt cctctggaag cttcttgaag
     3541 acaaacaacg tctgtagcga ccctttgcag gcagcggaac cccccacctg gcgacaggtg
     3601 cctctgcggc caaaagccac gtgtataaga tacacctgca aaggcggcac aaccccagtg
     3661 ccacgttgtg agttggatag ttgtggaaag agtcaaatgg ctctcctcaa gcgtattcaa
     3721 caaggggctg aaggatgccc agaaggtacc ccattgtatg ggatctgatc tggggcctcg
     3781 gtgcacatgc tttacatgtg tttagtcgag gttaaaaaac gtctaggccc cccgaaccac
     3841 ggggacgtgg ttttcctttg aaaaacacga tgataatatg gccacaacca tggtgagcaa
     3901 gggcgaggag ctgttcaccg gggtggtgcc catcctggtc gagctggacg gcgacgtaaa
     3961 cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg gcaagctgac
     4021 cctgaagttc atctgcacca ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac
     4081 cttcacctac ggcgtgcagt gcttcagccg ctaccccgac cacatgaagc agcacgactt
     4141 cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc accatcttct tcaaggacga
     4201 cggcaactac aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat
     4261 cgagctgaag ggcatcgact tcaaggagga cggcaacatc ctggggcaca agctggagta
     4321 caactacaac agccacaacg tctatatcat ggccgacaag cagaagaacg gcatcaaggt
     4381 gaacttcaag atccgccaca acatcgagga cggcagcgtg cagctcgccg accactacca
     4441 gcagaacacc cccatcggcg acggccccgt gctgctgccc gacaaccact acctgagcac
     4501 ccagtccgcc ctgagcaaag accccaacga gaagcgcgat cacatggtcc tgctggagtt
     4561 cgtgaccgcc gccgggatca ctctcggcat ggacgagctg tacaagtaat gaattaatta
     4621 agaattccgg attagtccaa tttgttaaag acaggatatc agtggtccag gctctagttt
     4681 tgactcaaca atatcaccag ctgaagccta tagagtacga gccatagata aaataaaaga
     4741 ttttatttag tctccagaaa aaggggggaa tgaaagaccc cacctgtagg tttggcaagc
     4801 tagcttaagt aacgccattt tgcaaggcat ggaaaataca taactgagaa tagagaagtt
     4861 cagatcaagg ttaggaacag agagacagca gaatatgggc caaacaggat atctgtggta
     4921 agcagttcct gccccgctca gggccaagaa cagttggaac aggagaatat gggccaaaca
     4981 ggatatctgt ggtaagcagt tcctgccccg gctcagggcc aagaacagat ggtccccaga
     5041 tgcggtcccg ccctcagcag tttctagaga accatcagat gtttccaggg tgccccaagg
     5101 acctgaaatg accctgtgcc ttatttgaac taaccaatca gttcgcttct cgcttctgtt
     5161 cgcgcgcttc tgctccccga gctcaataaa agagcccaca acccctcact cggcgcgcca
     5221 gtcctccgat agactgcgtc gcccgggtac ccgtgttctc aataaaccct cttgcagttg
     5281 catccgactc gtggtctcgc tgttccttgg gagggtctcc tctgagtgat tgactacccg
     5341 tcagcggggt ctttcagttt ctcccaccta cacaggtctc actaacattc ctgatgtgcc
     5401 gcagggactc cgtcagcccg gtttttgttt ataataaaat gcaagaacag tgttcccttc
     5461 aagccagact acatcctgac tctcggcttt ataaaagaat gttgaagggc tctgtggact
     5521 atctgccaca cgacttttta agatttttat gcctcctgga tgagggattt agtcaatcta
     5581 tcctcgtcta ttttgctggc ttctccgtat tttaaatttc tagtttgcac tcccttcctg
     5641 agagcacggc gattgcagag tagttaatac tctgagggca ggcttctgtg aaaaggttgc
     5701 ctgggctcag tgtgagattt tgccataaaa aggggtcctg cccctgtgta cagacagatc
     5761 ggaatctaga gtgcatactc agagtccccg cggttccggg gctctgatct cagggcatct
     5821 ttgcctagag atcctctacg ccggacgcat cgtggccctc gaattcgtaa tcatggtcat
     5881 agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa
     5941 gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc
     6001 gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc
     6061 aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact
     6121 cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac
     6181 ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa
     6241 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg
     6301 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa
     6361 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc
     6421 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac
     6481 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac
     6541 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg
     6601 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt
     6661 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagga
     6721 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct
     6781 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga
     6841 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg
     6901 ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct
     6961 tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt
     7021 aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc
     7081 tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg
     7141 gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag
     7201 atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt
     7261 tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag
     7321 ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt
     7381 ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca
     7441 tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg
     7501 ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat
     7561 ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta
     7621 tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca
     7681 gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct
     7741 taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat
     7801 cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa
     7861 agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt
     7921 gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa
     7981 ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac gtctaagaaa
     8041 ccattattat catgacatta acctataaaa ataggcgtat cacgaggccc tttcgtctcg
     8101 cgcgtttcgg tgatgacggt gaaaacctct gacacatgca gctcccggag acggtcacag
     8161 cttgtctgta agcggatgcc gggagcagac aagcccgtca gggcgcgtca gcgggtgttg
     8221 gcgggtgtcg gggctggctt aactatgcgg catcagagca gattgtactg agagtgcacc
     8281 atatgcggtg tgaaataccg cacagatgcg taaggagaaa ataccgcatc aggcgccatt
     8341 cgccattcag gctgcgcaac tgttgggaag ggcgatcggt gcgggcctct tcgctattac
     8401 gccagctggc gaaaggggga tgtgctgcaa ggcgattaag ttgggtaacg ccagggtttt
     8461 cccagtcacg acgttgtaaa acgacggcca gtgcc
//
