LOCUS       sequence-46313-e        8596 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8596)
  AUTHORS   Pratt ZL, Zhang J, Sugden B
  TITLE     The Latent Membrane Protein 1 (LMP1) Oncogene of Epstein-Barr Virus 
            Can Simultaneously Induce and Inhibit Apoptosis in B Cells.
  JOURNAL   J Virol. 2012 Apr;86(8):4380-93. Epub 2012 Feb 8.
  PUBMED    22318153
REFERENCE   2  (bases 1 to 8596)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Virol."; 
            date: "2012-04"; volume: "86(8)"; pages: "4380-93. Epub 2012 Feb 8"
COMMENT     Sequence Label: pCMMP-NGFR:LMP1-IRES-mRFP
FEATURES             Location/Qualifiers
     source          1..8596
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        84..387
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        388..591
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     542..562
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     misc_feature    813..1170
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             1235..1651
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1581..1603
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1619..1635
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     regulatory      3291..3300
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    3454..4019
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    3470..4021
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(3614..3631)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     3841..3860
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             4043..4714
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /translation="SSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAKLK
                     VTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGVVT
                     VTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKMRL
                     KLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHSTG
                     A"
     CDS             4043..4714
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /note="mammalian codon-optimized"
                     /translation="SSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAKLK
                     VTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGVVT
                     VTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKMRL
                     KLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHSTG
                     A"
     primer_bind     complement(4167..4185)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     4435..4454
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     4629..4652
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     primer_bind     complement(5977..5993)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5977..5993)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5990..6012)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6001..6017
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6025..6031,6032..6049,6050..6055))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6070..6091
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6208..6225)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6379..6967)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6459..6478)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7138..7929,7930..7998))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7761..7780
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7999..8103)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     8171..8189
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(8227..8249)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     8349..8368
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8562..8584
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     8576..8593
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     8577..8593
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 aagcttgcat gcctgcaggt cgactagtta ttaatagtaa tcaattacgg ggtcattagt
       61 tcatagccca tattggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga
      121 ccgcccaacg acccccgccc cttgacgtca ataatgacgt atgttcccat agtaacgcca
      181 atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca
      241 gtacatcaag tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg
      301 cccgcctggc attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc
      361 tacgtattag tcatcgctat taccatggtg atgcggttgt ggcagtacat caatgggcgt
      421 ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt
      481 ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg
      541 acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc tcgtttagtg
      601 aaccggcgcc agtcctccga tagactgcgt cgcccgggta cccgtgttct caataaaccc
      661 tcttgcagtt gcatccgact cgtggtctcg ctgttccttg ggagggtctc ctctgagtga
      721 ttgactaccc gtcagcgggg tctttcattt ggaggttcca ccgagatttg gagacccctg
      781 cccagggacc accgaccccc ccgccgggag gtaagctggc cagcaactta tctgtgtctg
      841 tccgattgtc tagtgtctat gactgatttt atgcgcctgc gtcggtacta gttagctaac
      901 tagctctgta tctggcggac ccgtggtgga actgacgagt tcggaacacc cggccgcaac
      961 cctgggagac gtcccaggga cttcgggggc cgtttttgtg gcccgacctg agtcctaaaa
     1021 tcccgatcgt ttaggactct ttggtgcacc ccccttagag gagggatatg tggttctggt
     1081 aggagacgag aacctaaaac agttcccgcc tccgtctgaa tttttgcttt cggtttggga
     1141 ccgaagccgc gccgcgcgtc ttgtctgctg cagcatcgtt ctgtgttgtc tctgtctgac
     1201 tgtgtttctg tatttgtctg aaaatatggg cccgggctag actgttacca ctcccttaag
     1261 tttgacctta ggtcactgga aagatgtcga gcggatcgct cacaaccagt cggtagatgt
     1321 caagaagaga cgttgggtta ccttctgctc tgcagaatgg ccaaccttta acgtcggatg
     1381 gccgcgagac ggcaccttta accgagacct catcacccag gttaagatca aggtcttttc
     1441 acctggcccg catggacacc cagaccaggt cccctacatc gtgacctggg aagccttggc
     1501 ttttgacccc cctccctggg tcaagccctt tgtacaccct aagcctccgc ctcctcttcc
     1561 tccatccgcc ccgtctctcc cccttgaacc tcctcgttcg accccgcctc gatcctccct
     1621 ttatccagcc ctcactcctt ctctaggcgc ccccatatgg ccatatgaga tcttatatgg
     1681 ggcacccccg ccccttgtaa acttccctga ccctgacatg acaagagtta ctaacagccc
     1741 ctctctccaa gctcacttac aggctctcta cttagtccag cacgaagtct ggagacctct
     1801 ggcggcagcc taccaagaac aactggaccg accggtcaat tggttaacgc tgagcttcga
     1861 agccggcgcg gccgcacgcg tttccagaag tagtgaggag gcttttttgg aggcctaggc
     1921 ttttgcaaaa agcttgcatg cctgcaggtc gagcgctgcc gcgggaggcg ggcgatgggg
     1981 gcaggtgcca ccggccgcgc catggacggg ccgcgcctgc tgctgttgct gcttctgggg
     2041 gtgtcccttg gaggtgccaa ggaggcatgc cccacaggcc tgtacacaca cagcggtgag
     2101 tgctgcaaag cctgcaacct gggcgagggt gtggcccagc cttgtggagc caaccagacc
     2161 gtgtgtgagc cctgcctgga cagcgtgacg ttctccgacg tggtgagcgc gaccgagccg
     2221 tgcaagccgt gcaccgagtg cgtggggctc cagagcatgt cggcgccgtg cgtggaggcc
     2281 gacgacgccg tgtgccgctg cgcctacggc tactaccagg atgagacgac tgggcgctgc
     2341 gaggcgtgcc gcgtgtgcga ggcgggctcg ggcctcgtgt tctcctgcca ggacaagcag
     2401 aacaccgtgt gcgaggagtg ccccgacggc acgtattccg acgaggccaa ccacgtggac
     2461 ccgtgcctgc cctgcaccgt gtgcgaggac accgagcgcc agctccgcga gtgcacacgc
     2521 tgggccgacg ccgagtgcga ggagatccct ggccgttgga ttacacggtc cacaccccca
     2581 gagggctcgg acagcacagc ccccagcacc caggagcctg aggcacctcc agaacaagac
     2641 ctcatagcca gcacggtggc aggtgtggtg accacagtga tgggcagctc ccagcccgtg
     2701 gtgacccgag gcaccaccga caacctcatc cctgtctatt gctccatcct ggctgctgtg
     2761 gttgtgggcc ttgtggccta catagccttc aagaggtgga acagggggat cagtgatgaa
     2821 caccaccacg atgactccct cccgcaccct caacaagcta ccgatgattc tggccatgaa
     2881 tctgactcta actccaacga gggcagacac cacctgctcg tgagtggagc cggcgacgga
     2941 cccccactct gctctcaaaa cctaggcgca cctggaggtg gtcctgacaa tggcccacag
     3001 gaccctgaca acactgatga caatggccca caggaccctg acaacactga tgacaatggc
     3061 ccacatgacc cgctgcctca ggaccctgac aacactgatg acaatggccc acaggaccct
     3121 gacaacactg atgacaatgg cccacatgac ccgctgcctc atagccctag cgactctgct
     3181 ggaaatgatg gaggccctcc acaattgacg gaagaggttg aaaacaaagg aggtgaccag
     3241 ggcccgcctt tgatgacaga cggaggcggc ggtcatagtc atgattccgg ccatggcggc
     3301 ggtgatccac accttcctac gctgcttttg ggttcttctg gttccggtgg agatgatgac
     3361 gacccccacg gcccagttca gctaagctac tatgactaac ctttctttac ttctaggcat
     3421 taccatgtca taggcttctc gaggatccgg aattccgccc ccccccctaa cgttactggc
     3481 cgaagccgct tggaataagg ccggtgtgcg tttgtctata tgttattttc caccatattg
     3541 ccgtcttttg gcaatgtgag ggcccggaaa cctggccctg tcttcttgac gagcattcct
     3601 aggggtcttt cccctctcgc caaaggaatg caaggtctgt tgaatgtcgt gaaggaagca
     3661 gttcctctgg aagcttcttg aagacaaaca acgtctgtag cgaccctttg caggcagcgg
     3721 aaccccccac ctggcgacag gtgcctctgc ggccaaaagc cacgtgtata agatacacct
     3781 gcaaaggcgg cacaacccca gtgccacgtt gtgagttgga tagttgtgga aagagtcaaa
     3841 tggctctcct caagcgtatt caacaagggg ctgaaggatg cccagaaggt accccattgt
     3901 atgggatctg atctggggcc tcggtgcaca tgctttacat gtgtttagtc gaggttaaaa
     3961 aacgtctagg ccccccgaac cacggggacg tggttttcct ttgaaaaaca cgatgataat
     4021 atggccacaa ccatggttca cctcctccga ggacgtcatc aaggagttca tgcgcttcaa
     4081 ggtgcgcatg gagggctccg tgaacggcca cgagttcgag atcgagggcg agggcgaggg
     4141 ccgcccctac gagggcaccc agaccgccaa gctgaaggtg accaagggcg gccccctgcc
     4201 cttcgcctgg gacatcctgt cccctcagtt ccagtacggc tccaaggcct acgtgaagca
     4261 ccccgccgac atccccgact acttgaagct gtccttcccc gagggcttca agtgggagcg
     4321 cgtgatgaac ttcgaggacg gcggcgtggt gaccgtgacc caggactcct ccctgcagga
     4381 cggcgagttc atctacaagg tgaagctgcg cggcaccaac ttcccctccg acggccccgt
     4441 aatgcagaag aagaccatgg gctgggaggc ctccaccgag cggatgtacc ccgaggacgg
     4501 cgccctgaag ggcgagatca agatgaggct gaagctgaag gacggcggcc actacgacgc
     4561 cgaggtcaag accacctaca tggccaagaa gcccgtgcag ctgcccggcg cctacaagac
     4621 cgacatcaag ctggacatca cctcccacaa cgaggactac accatcgtgg aacagtacga
     4681 gcgcgccgag ggccgccact ccaccggcgc ctaattaatt aagaattccg gattagtcca
     4741 atttgttaaa gacaggatat cagtggtcca ggctctagtt ttgactcaac aatatcacca
     4801 gctgaagcct atagagtacg agccatagat aaaataaaag attttattta gtctccagaa
     4861 aaagggggga atgaaagacc ccacctgtag gtttggcaag ctagcttaag taacgccatt
     4921 ttgcaaggca tggaaaatac ataactgaga atagagaagt tcagatcaag gttaggaaca
     4981 gagagacagc agaatatggg ccaaacagga tatctgtggt aagcagttcc tgccccgctc
     5041 agggccaaga acagttggaa caggagaata tgggccaaac aggatatctg tggtaagcag
     5101 ttcctgcccc ggctcagggc caagaacaga tggtccccag atgcggtccc gccctcagca
     5161 gtttctagag aaccatcaga tgtttccagg gtgccccaag gacctgaaat gaccctgtgc
     5221 cttatttgaa ctaaccaatc agttcgcttc tcgcttctgt tcgcgcgctt ctgctccccg
     5281 agctcaataa aagagcccac aacccctcac tcggcgcgcc agtcctccga tagactgcgt
     5341 cgcccgggta cccgtgttct caataaaccc tcttgcagtt gcatccgact cgtggtctcg
     5401 ctgttccttg ggagggtctc ctctgagtga ttgactaccc gtcagcgggg tctttcagtt
     5461 tctcccacct acacaggtct cactaacatt cctgatgtgc cgcagggact ccgtcagccc
     5521 ggtttttgtt tataataaaa tgcaagaaca gtgttccctt caagccagac tacatcctga
     5581 ctctcggctt tataaaagaa tgttgaaggg ctctgtggac tatctgccac acgacttttt
     5641 aagattttta tgcctcctgg atgagggatt tagtcaatct atcctcgtct attttgctgg
     5701 cttctccgta ttttaaattt ctagtttgca ctcccttcct gagagcacgg cgattgcaga
     5761 gtagttaata ctctgagggc aggcttctgt gaaaaggttg cctgggctca gtgtgagatt
     5821 ttgccataaa aaggggtcct gcccctgtgt acagacagat cggaatctag agtgcatact
     5881 cagagtcccc gcggttccgg ggctctgatc tcagggcatc tttgcctaga gatcctctac
     5941 gccggacgca tcgtggccct cgaattcgta atcatggtca tagctgtttc ctgtgtgaaa
     6001 ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg
     6061 gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca
     6121 gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg
     6181 tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg
     6241 gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg
     6301 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa
     6361 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg
     6421 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
     6481 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
     6541 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc
     6601 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
     6661 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc
     6721 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga
     6781 gttcttgaag tggtggccta actacggcta cactagaagg acagtatttg gtatctgcgc
     6841 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac
     6901 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg
     6961 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc
     7021 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa
     7081 ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta
     7141 ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt
     7201 tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag
     7261 tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag caataaacca
     7321 gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc
     7381 tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt
     7441 tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag
     7501 ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt
     7561 tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat
     7621 ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt
     7681 gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc
     7741 ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat
     7801 cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag
     7861 ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt
     7921 ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg
     7981 gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta
     8041 ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc
     8101 gcgcacattt ccccgaaaag tgccacctga cgtctaagaa accattatta tcatgacatt
     8161 aacctataaa aataggcgta tcacgaggcc ctttcgtctc gcgcgtttcg gtgatgacgg
     8221 tgaaaacctc tgacacatgc agctcccgga gacggtcaca gcttgtctgt aagcggatgc
     8281 cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc ggggctggct
     8341 taactatgcg gcatcagagc agattgtact gagagtgcac catatgcggt gtgaaatacc
     8401 gcacagatgc gtaaggagaa aataccgcat caggcgccat tcgccattca ggctgcgcaa
     8461 ctgttgggaa gggcgatcgg tgcgggcctc ttcgctatta cgccagctgg cgaaaggggg
     8521 atgtgctgca aggcgattaa gttgggtaac gccagggttt tcccagtcac gacgttgtaa
     8581 aacgacggcc agtgcc
//
