LOCUS       sequence-463137-         702 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  tagBFP expression to serve as a ""filler"" plasmid for 
            transfections.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 702)
  AUTHORS   Love KS, Johnstone CP, Peterman EL, Gaglione S, Birnbaum ME, 
            Galloway KE
  TITLE     Model-guided design of microRNA-based gene circuits supports precise
            dosage of transgenic cargoes into diverse primary cells.
  JOURNAL   Cell Syst. 2025 Apr 22:101269. doi: 10.1016/j.cels.2025.101269.
  PUBMED    40300600
REFERENCE   2  (bases 1 to 702)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Syst. 
            2025 Apr 22:101269. doi: 10.1016/j.cels.2025.101269."
COMMENT     Sequence Label: EF1a-tagBFP-bGH
FEATURES             Location/Qualifiers
     source          1..702
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1..702
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKV
                     VEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA
                     TQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALK
                     LVGGSHLIANIKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYC
                     DLPSKLGHKLN"
ORIGIN
        1 atgagcgagc tgattaagga gaacatgcac atgaagctgt acatggaggg caccgtggac
       61 aaccatcact tcaagtgcac atccgagggc gaaggcaagc cctacgaggg cacccagacc
      121 atgagaatca aggtggtcga gggcggccct ctccccttcg ccttcgacat cctggctact
      181 agcttcctct acggcagcaa gaccttcatc aaccacaccc agggcatccc cgacttcttc
      241 aagcagtcct tccctgaggg cttcacatgg gagagagtca ccacatacga agacgggggc
      301 gtgctgaccg ctacccagga caccagcctc caggacggct gcctcatcta caacgtcaag
      361 atcagagggg tgaacttcac atccaacggc cctgtgatgc agaagaaaac actcggctgg
      421 gaggccttca ccgagacgct gtaccccgct gacggcggcc tggaaggcag aaacgacatg
      481 gccctgaagc tcgtgggcgg gagccatctg atcgcaaaca tcaagaccac atatagatcc
      541 aagaaacccg ctaagaacct caagatgcct ggcgtctact atgtggacta cagactggaa
      601 agaatcaagg aggccaacaa cgaaacgtac gtcgagcagc acgaggtggc agtggccaga
      661 tactgcgacc tccctagcaa actggggcac aagcttaatt aa
//
