LOCUS       sequence-463151-        1137 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  microRNA expression for dual-vector iFFL circuit.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1137)
  AUTHORS   Love KS, Johnstone CP, Peterman EL, Gaglione S, Birnbaum ME, 
            Galloway KE
  TITLE     Model-guided design of microRNA-based gene circuits supports precise
            dosage of transgenic cargoes into diverse primary cells.
  JOURNAL   Cell Syst. 2025 Apr 22:101269. doi: 10.1016/j.cels.2025.101269.
  PUBMED    40300600
REFERENCE   2  (bases 1 to 1137)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Syst. 
            2025 Apr 22:101269. doi: 10.1016/j.cels.2025.101269."
COMMENT     Sequence Label: EF1a-mGL(miRE.FF4)-bGH
FEATURES             Location/Qualifiers
     source          1..1137
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(46..67)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     ncRNA           527..541
                     /label=miR-30a loop
                     /note="loop from the the 71-nt precursor of the human 
                     miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     ncRNA           598..710
                     /label=3' miR-30a
                     /note="sequence downstream of the 71-nt precursor of the 
                     human miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
ORIGIN
        1 atggtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac
       61 ggcgacgtaa acggccacaa gttcagcgtc cgcggcgagg gcgagggcga tgccaccaac
      121 ggcaagctga ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc
      181 ctcgtgacca ccttaggcta cggcgtggcc tgcttcgccc gctaccccga ccacatgaag
      241 cagcacgact tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatctct
      301 ttcaaggacg acggtaccta caagacccgc gccgaggtaa gtgtgctcgc ttcggcagca
      361 catatactat gttgaatgag gcttcagtac tttacagaat cgttgcctgc acatcttgga
      421 aacacttgct gggattactt cttcagggac ttcttaaccc aacagaaggc tcgagaaggt
      481 atattgctgt tgacagtgag cgccgcttga agtctttaat taaatagtga agccacagat
      541 gtatttaatt aaagacttca agcggtgcct actgcctcgg acttcaaggg gctagaattc
      601 aaggggctac tttaggagca attatcttgt ttactaaaac tgaatacctt gctatctctt
      661 tgatacattt ttacaaagct gaattaaaat ggtataaatt aaatcacttt tttcaattgt
      721 actaacttcg agtcttcttt ttttttttca caggtgaagt tcgagggcga caccctggtg
      781 aaccgcatcg tgctgaaggg catcgacttc aaggaggacg gcaacatcct ggggcacaag
      841 ctggagtaca acttcaacag ccacaaggtc tatatcacgg ccgacaagca gaagaacggc
      901 atcaaggcta acttcaagac ccgccacaac gttgaggacg gcggcgtgca gctcgccgac
      961 cactaccagc agaacacccc catcggcgac ggccccgtgc tgctgcccga caaccactac
     1021 ctgagccatc agtccaaact gagcaaagac cccaacgaga agcgcgatca catggtcctg
     1081 aaggagaggg tgaccgccgc cgggattaca catgacatgg acgagctgta caagtaa
//
