LOCUS       sequence-463194-        1088 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  ComMAND closed-loop circuit regulating mRuby2 with miR-FF4 design 2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1088)
  AUTHORS   Love KS, Johnstone CP, Peterman EL, Gaglione S, Birnbaum ME, 
            Galloway KE
  TITLE     Model-guided design of microRNA-based gene circuits supports precise
            dosage of transgenic cargoes into diverse primary cells.
  JOURNAL   Cell Syst. 2025 Apr 22:101269. doi: 10.1016/j.cels.2025.101269.
  PUBMED    40300600
REFERENCE   2  (bases 1 to 1088)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Syst. 
            2025 Apr 22:101269. doi: 10.1016/j.cels.2025.101269."
COMMENT     Sequence Label: EF1a-TS.FF4x1-mRuby2(miR.FF4)-bGH
FEATURES             Location/Qualifiers
     source          1..1088
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     ncRNA           87..180
                     /label=5' miR-30a
                     /note="sequence upstream of the 71-nt precursor of the 
                     human miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     ncRNA           227..241
                     /label=miR-30a loop
                     /note="loop from the the 71-nt precursor of the human 
                     miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     ncRNA           269..397
                     /label=3' miR-30a
                     /note="sequence downstream of the 71-nt precursor of the 
                     human miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
ORIGIN
        1 atggtgtcta agggcgaaga gctgatcaag gaaaatatgc gtatgaaggt gagtatgtgc
       61 tcgcttcggc agcacatata ctatgtttga atgaggcttc agtactttac agaatcgttg
      121 cctgcacatc ttggaaacac ttgctgggat tacttcttca ggttaaccca acagaaggct
      181 cgagtgctgt tgacagtgag cgccgcttga agtctttaat taaatagtga agccacagat
      241 gtatttaatt aaagacttca agcggtgcct actgcctcgg agaattcaag gggctacttt
      301 aggagcaatt atcttgttta ctaaaactga ataccttgct atctctttga tacattttta
      361 caaagctgaa ttaaaatggt ataaattaaa tcactttttt caattgtttc cttttttttc
      421 ctcaggtggt catggaaggt tcggtcaacg gccaccaatt caaatgcaca ggtgaaggag
      481 aaggcaatcc gtacatggga actcaaacca tgaggatcaa agtcatcgag ggaggacccc
      541 tgccatttgc ctttgacatt cttgccacgt cgttcatgta tggcagccgt acttttatca
      601 agtacccgaa aggcattcct gatttcttta aacagtcctt tcctgagggt tttacttggg
      661 aaagagttac gagatacgaa gatggtggag tcgtcaccgt catgcaggac accagccttg
      721 aggatggctg tctcgtttac cacgtccaag tcagaggggt aaactttccc tccaatggtc
      781 ccgtgatgca gaagaagacc aagggttggg agcctaatac agagatgatg tatccagcag
      841 atggtggtct gaggggatac actcatatgg cactgaaagt tgatggtggt ggccatctgt
      901 cttgctcttt cgtaacaact tacaggtcaa aaaagaccgt cgggaacatc aagatgcccg
      961 gtatccatgc cgttgatcac cgcctggaaa ggttagagga aagtgacaat gaaatgttcg
     1021 tagtacaacg cgaacacgca gttgccaagt tcgccgggct tggtggtggg atggacgagc
     1081 tgtacaag
//
