LOCUS       sequence-463212-        1128 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  ComMAND closed-loop circuit regulating mRuby2 with 2 copies of 
            microRNA target sites.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1128)
  AUTHORS   Love KS, Johnstone CP, Peterman EL, Gaglione S, Birnbaum ME, 
            Galloway KE
  TITLE     Model-guided design of microRNA-based gene circuits supports precise
            dosage of transgenic cargoes into diverse primary cells.
  JOURNAL   Cell Syst. 2025 Apr 22:101269. doi: 10.1016/j.cels.2025.101269.
  PUBMED    40300600
REFERENCE   2  (bases 1 to 1128)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Syst. 
            2025 Apr 22:101269. doi: 10.1016/j.cels.2025.101269."
COMMENT     Sequence Label: EF1a-mRuby2(miRE.FF4)-TS.FF4x2-bGH
FEATURES             Location/Qualifiers
     source          1..1128
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     ncRNA           239..253
                     /label=miR-30a loop
                     /note="loop from the the 71-nt precursor of the human 
                     miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     ncRNA           310..422
                     /label=3' miR-30a
                     /note="sequence downstream of the 71-nt precursor of the 
                     human miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
ORIGIN
        1 atggtgtcta agggcgaaga gctgatcaag gaaaatatgc gtatgaaggt aagtgtgctc
       61 gcttcggcag cacatatact atgttgaatg aggcttcagt actttacaga atcgttgcct
      121 gcacatcttg gaaacacttg ctgggattac ttcttcaggg acttcttaac ccaacagaag
      181 gctcgagaag gtatattgct gttgacagtg agcgccgctt gaagtcttta attaaatagt
      241 gaagccacag atgtatttaa ttaaagactt caagcggtgc ctactgcctc ggacttcaag
      301 gggctagaat tcaaggggct actttaggag caattatctt gtttactaaa actgaatacc
      361 ttgctatctc tttgatacat ttttacaaag ctgaattaaa atggtataaa ttaaatcact
      421 tttttcaatt gtactaactt cgagtcttct tttttttttt cacaggtggt catggaaggt
      481 tcggtcaacg gccaccaatt caaatgcaca ggtgaaggag aaggcaatcc gtacatggga
      541 actcaaacca tgaggatcaa agtcatcgag ggaggacccc tgccatttgc ctttgacatt
      601 cttgccacgt cgttcatgta tggcagccgt acttttatca agtacccgaa aggcattcct
      661 gatttcttta aacagtcctt tcctgagggt tttacttggg aaagagttac gagatacgaa
      721 gatggtggag tcgtcaccgt catgcaggac accagccttg aggatggctg tctcgtttac
      781 cacgtccaag tcagaggggt aaactttccc tccaatggtc ccgtgatgca gaagaagacc
      841 aagggttggg agcctaatac agagatgatg tatccagcag atggtggtct gaggggatac
      901 actcatatgg cactgaaagt tgatggtggt ggccatctgt cttgctcttt cgtaacaact
      961 tacaggtcaa aaaagaccgt cgggaacatc aagatgcccg gtatccatgc cgttgatcac
     1021 cgcctggaaa ggttagagga aagtgacaat gaaatgttcg tagtacaacg cgaacacgca
     1081 gttgccaagt tcgccgggct tggtggtggg atggacgagc tgtacaag
//
