LOCUS       sequence-46322-e         994 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 994)
  AUTHORS   Bracken CP, Gregory PA, Kolesnikoff N, Bert AG, Wang J, Shannon MF, 
            Goodall GJ
  TITLE     A double-negative feedback loop between ZEB1-SIP1 and the 
            microRNA-200 family regulates epithelial-mesenchymal transition.
  JOURNAL   Cancer Res. 2008 Oct 1;68(19):7846-54.
  PUBMED    18829540
REFERENCE   2  (bases 1 to 994)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cancer 
            Res."; date: "2008-10-1"; volume: "68(19)"; pages: "7846-54"
COMMENT     Sequence Label: pGL3-321/+120 mut1&2
FEATURES             Location/Qualifiers
     source          1..994
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      35..44
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    565..656
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     complement(597..616)
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
     polyA_signal    670..718
                     /note="synthetic polyadenylation signal"
ORIGIN
        1 ggcgccgggc ctttctttat gtttttggcg tcttccatgg tggctttacc aacagtaccg
       61 gaatgccaag cttacttaga tcgcagatct cctggcacag gaagtcagtt cagacctgca
      121 caggtggaca actcgcccgt ctctgggaga gtttcgaggg cgacgggaca atgggaggcg
      181 gtggcgaggt gggggcctcg ggagggaaga gccggctcgg cgggggctcg ccttacaagg
      241 agcagtgctc cccgccccac accggtgcga gctcccgctg gccaatctcc gctcctggcc
      301 cccgaccggt gtgtcccctg ggctcccata ggccgggcgg gaggcggacc agtgacggtg
      361 acgcaggccc gggccccgca ggccccgcag actccgtccc gcccccacct tgccgcccac
      421 cgaggttgaa caaaagccag gatgggaagg cttctgtggg gcccgtgtgg agtgtgggcg
      481 acatgggcag gggtaggggc agccgcgggc aactctcgcc tctccacctc tgctagcacg
      541 cgtaagagct cggtacctat cgatagagaa atgttctggc acctgcactt gcactgggga
      601 cagcctattt tgctagtttg ttttgtttcg ttttgttttg atggagagcg tatgttagta
      661 ctatcgattc acacaaaaaa ccaacacaca gatgtaatga aaataaagat attttattgc
      721 ggccgctcca agtacctccc gtaccttaat attacttact tatcatggta gcttgggctg
      781 gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg
      841 cgaatggcaa attgtaagcg ttaatatttt gttaaaattc gcgttaaatt tttgttaaat
      901 cagctcattt tttaaccaat aggccgaaat cggcaaaatc ccttataaat caaaagaata
      961 gaccgagata gggttgagtg ttgttccagt ttgg
//
