LOCUS       sequence-463239-         630 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral expression of ComMAND closed-loop circuit regulating 
            FXN-P2A-mRuby2 (therapeutically relevant gene).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 630)
  AUTHORS   Love KS, Johnstone CP, Peterman EL, Gaglione S, Birnbaum ME, 
            Galloway KE
  TITLE     Model-guided design of microRNA-based gene circuits supports precise
            dosage of transgenic cargoes into diverse primary cells.
  JOURNAL   Cell Syst. 2025 Apr 22:101269. doi: 10.1016/j.cels.2025.101269.
  PUBMED    40300600
REFERENCE   2  (bases 1 to 630)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Syst. 
            2025 Apr 22:101269. doi: 10.1016/j.cels.2025.101269."
COMMENT     Sequence Label: 
            pLentiX1-[TRE3G-FXN-P2A-mRuby2(miRE.FF4)-TS.FF4x1-bGH]-EFS-rtTA-P2A-
            mGL-WPRE
FEATURES             Location/Qualifiers
     source          1..630
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 atgtggactc tcgggcgccg cgcagtagcc ggcctcctgg cgtcacccag cccggcccag
       61 gcccagaccc tcacccgggt cccgcggccg gcagagttgg ccccactctg cggccgccgt
      121 ggcctgcgca ccgacatcga tgcgacctgc acgccccgcc gcgcaagttc gaaccaacgt
      181 ggcctcaacc agatttggaa tgtcaaaaag cagagtgtct atttgatgaa tttgaggaaa
      241 tctggaactt tgggccaccc aggctctcta gatgagacca cctatgaaag actagcagag
      301 gaaacgctgg actctttagc agagtttttt gaagaccttg cagacaagcc atacacgttt
      361 gaggactatg atgtctcctt tgggagtggt gtcttaactg tcaaactggg tggagatcta
      421 ggaacctatg tgatcaacaa gcagacgcca aacaagcaaa tctggctatc ttctccatcc
      481 agtggaccta agcgttatga ctggactggg aaaaactggg tgtactccca cgacggcgtg
      541 tccctccatg agctgctggc cgcagagctc actaaagcct taaaaaccaa actggacttg
      601 tcttccttgg cctattccgg aaaagatgct
//
