LOCUS       sequence-463262-         711 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral expression of mRuby2 with 3' microRNA target site only 
            (closed-loop).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 711)
  AUTHORS   Love KS, Johnstone CP, Peterman EL, Gaglione S, Birnbaum ME, 
            Galloway KE
  TITLE     Model-guided design of microRNA-based gene circuits supports precise
            dosage of transgenic cargoes into diverse primary cells.
  JOURNAL   Cell Syst. 2025 Apr 22:101269. doi: 10.1016/j.cels.2025.101269.
  PUBMED    40300600
REFERENCE   2  (bases 1 to 711)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Syst. 
            2025 Apr 22:101269. doi: 10.1016/j.cels.2025.101269."
COMMENT     Sequence Label: 
            pLentiX1-[TRE3G-mRuby2-TS-FF4x1-bGH]-EFS-rtTA-T2A-mGL-P2A-PuroR-WPRE
FEATURES             Location/Qualifiers
     source          1..711
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1..711
                     /codon_start=1
                     /product="monomeric red fluorescent protein derived from 
                     mRuby, with improved photophysical properties (Lam et al., 
                     2012)"
                     /label=mRuby2
                     /translation="MVSKGEELIKENMRMKVVMEGSVNGHQFKCTGEGEGNPYMGTQTM
                     RIKVIEGGPLPFAFDILATSFMYGSRTFIKYPKGIPDFFKQSFPEGFTWERVTRYEDGG
                     VVTVMQDTSLEDGCLVYHVQVRGVNFPSNGPVMQKKTKGWEPNTEMMYPADGGLRGYTH
                     MALKVDGGGHLSCSFVTTYRSKKTVGNIKMPGIHAVDHRLERLEESDNEMFVVQREHAV
                     AKFAGLGGGMDELYK"
ORIGIN
        1 atggtgtcta agggcgaaga gctgatcaag gaaaatatgc gtatgaaggt ggtcatggaa
       61 ggttcggtca acggccacca attcaaatgc acaggtgaag gagaaggcaa tccgtacatg
      121 ggaactcaaa ccatgaggat caaagtcatc gagggaggac ccctgccatt tgcctttgac
      181 attcttgcca cgtcgttcat gtatggcagc cgtactttta tcaagtaccc gaaaggcatt
      241 cctgatttct ttaaacagtc ctttcctgag ggttttactt gggaaagagt tacgagatac
      301 gaagatggtg gagtcgtcac cgtcatgcag gacaccagcc ttgaggatgg ctgtctcgtt
      361 taccacgtcc aagtcagagg ggtaaacttt ccctccaatg gtcccgtgat gcagaagaag
      421 accaagggtt gggagcctaa tacagagatg atgtatccag cagatggtgg tctgagggga
      481 tacactcata tggcactgaa agttgatggt ggtggccatc tgtcttgctc tttcgtaaca
      541 acttacaggt caaaaaagac cgtcgggaac atcaagatgc ccggtatcca tgccgttgat
      601 caccgcctgg aaaggttaga ggaaagtgac aatgaaatgt tcgtagtaca acgcgaacac
      661 gcagttgcca agttcgccgg gcttggtggt gggatggacg agctgtacaa g
//
