LOCUS       sequence-463263-         744 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral expression of mRuby2 with 3' microRNA target site only 
            (closed-loop).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 744)
  AUTHORS   Love KS, Johnstone CP, Peterman EL, Gaglione S, Birnbaum ME, 
            Galloway KE
  TITLE     Model-guided design of microRNA-based gene circuits supports precise
            dosage of transgenic cargoes into diverse primary cells.
  JOURNAL   Cell Syst. 2025 Apr 22:101269. doi: 10.1016/j.cels.2025.101269.
  PUBMED    40300600
REFERENCE   2  (bases 1 to 744)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Syst. 
            2025 Apr 22:101269. doi: 10.1016/j.cels.2025.101269."
COMMENT     Sequence Label: 
            pLentiX1-[TRE3G-mRuby2-TS-FF4x1-bGH]-EFS-rtTA-T2A-mGL-P2A-PuroR-WPRE
FEATURES             Location/Qualifiers
     source          1..744
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1..744
                     /codon_start=1
                     /product="modified rtTA protein that binds tightly to 
                     promoters containing the tet operator in the presence of 
                     doxycycline"
                     /label=Tet-On(R) 3G
                     /translation="MSRLDKSKVINSALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHSCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLKQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
ORIGIN
        1 atgagccggc tggacaagag caaagtgatc aacagcgccc tggaactgct gaacggcgtg
       61 ggcatcgagg gcctgaccac ccggaagctg gcccagaaac tgggcgtgga acagcccacc
      121 ctgtactggc acgtgaagaa caagcgggcc ctgctggacg ccctgcccat cgagatgctg
      181 gaccggcacc acacccacag ctgccctctg gaaggcgaga gctggcagga cttcctgcgg
      241 aacaacgcca agagctacag atgcgccctg ctgagccacc gggacggcgc caaagtgcac
      301 ctgggcacca gacccaccga gaagcagtac gagacactgg aaaaccagct ggccttcctg
      361 tgccagcagg gcttcagcct ggaaaacgcc ctgtacgccc tgagcgccgt gggccacttc
      421 accctgggct gcgtgctgga agaacaggaa caccaggtcg ccaaagagga aagagagaca
      481 cccaccaccg acagcatgcc ccccctgctg aagcaggcca tcgagctgtt cgacagacag
      541 ggcgccgagc ccgccttcct gttcggcctg gaactgatca tctgcggcct cgagaagcag
      601 ctgaagtgcg agagcggcgg acccaccgac gccctggacg acttcgacct ggacatgctg
      661 cccgccgatg ccctggatga ttttgatctg gatatgctcc ctgctgacgc tctcgatgac
      721 ttcgatctcg atatgctgcc aggc
//
