LOCUS       sequence-463274-        1137 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral expression of mRuby2 with 3' microRNA only.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1137)
  AUTHORS   Love KS, Johnstone CP, Peterman EL, Gaglione S, Birnbaum ME, 
            Galloway KE
  TITLE     Model-guided design of microRNA-based gene circuits supports precise
            dosage of transgenic cargoes into diverse primary cells.
  JOURNAL   Cell Syst. 2025 Apr 22:101269. doi: 10.1016/j.cels.2025.101269.
  PUBMED    40300600
REFERENCE   2  (bases 1 to 1137)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Syst. 
            2025 Apr 22:101269. doi: 10.1016/j.cels.2025.101269."
COMMENT     Sequence Label: 
            pLentiX1-[TRE3G-mRuby2-miRE.FF4-bGH]-EFS-rtTA-T2A-mGL-P2A-PuroR-WPRE
FEATURES             Location/Qualifiers
     source          1..1137
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1..711
                     /codon_start=1
                     /product="monomeric red fluorescent protein derived from 
                     mRuby, with improved photophysical properties (Lam et al., 
                     2012)"
                     /label=mRuby2
                     /translation="MVSKGEELIKENMRMKVVMEGSVNGHQFKCTGEGEGNPYMGTQTM
                     RIKVIEGGPLPFAFDILATSFMYGSRTFIKYPKGIPDFFKQSFPEGFTWERVTRYEDGG
                     VVTVMQDTSLEDGCLVYHVQVRGVNFPSNGPVMQKKTKGWEPNTEMMYPADGGLRGYTH
                     MALKVDGGGHLSCSFVTTYRSKKTVGNIKMPGIHAVDHRLERLEESDNEMFVVQREHAV
                     AKFAGLGGGMDELYK"
     ncRNA           911..925
                     /label=miR-30a loop
                     /note="loop from the the 71-nt precursor of the human 
                     miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     ncRNA           982..1094
                     /label=3' miR-30a
                     /note="sequence downstream of the 71-nt precursor of the 
                     human miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
ORIGIN
        1 atggtgtcta agggcgaaga gctgatcaag gaaaatatgc gtatgaaggt ggtcatggaa
       61 ggttcggtca acggccacca attcaaatgc acaggtgaag gagaaggcaa tccgtacatg
      121 ggaactcaaa ccatgaggat caaagtcatc gagggaggac ccctgccatt tgcctttgac
      181 attcttgcca cgtcgttcat gtatggcagc cgtactttta tcaagtaccc gaaaggcatt
      241 cctgatttct ttaaacagtc ctttcctgag ggttttactt gggaaagagt tacgagatac
      301 gaagatggtg gagtcgtcac cgtcatgcag gacaccagcc ttgaggatgg ctgtctcgtt
      361 taccacgtcc aagtcagagg ggtaaacttt ccctccaatg gtcccgtgat gcagaagaag
      421 accaagggtt gggagcctaa tacagagatg atgtatccag cagatggtgg tctgagggga
      481 tacactcata tggcactgaa agttgatggt ggtggccatc tgtcttgctc tttcgtaaca
      541 acttacaggt caaaaaagac cgtcgggaac atcaagatgc ccggtatcca tgccgttgat
      601 caccgcctgg aaaggttaga ggaaagtgac aatgaaatgt tcgtagtaca acgcgaacac
      661 gcagttgcca agttcgccgg gcttggtggt gggatggacg agctgtacaa gtaacaacag
      721 gtaagtgtgc tcgcttcggc agcacatata ctatgttgaa tgaggcttca gtactttaca
      781 gaatcgttgc ctgcacatct tggaaacact tgctgggatt acttcttcag ggacttctta
      841 acccaacaga aggctcgaga aggtatattg ctgttgacag tgagcgccgc ttgaagtctt
      901 taattaaata gtgaagccac agatgtattt aattaaagac ttcaagcggt gcctactgcc
      961 tcggacttca aggggctaga attcaagggg ctactttagg agcaattatc ttgtttacta
     1021 aaactgaata ccttgctatc tctttgatac atttttacaa agctgaatta aaatggtata
     1081 aattaaatca cttttttcaa ttgtactaac ttcgagtctt cttttttttt ttcacag
//
