LOCUS       sequence-463314-       10154 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Protein expression in insect cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10154)
  AUTHORS   Lopez Martinez D, Todorovski I, Noe Gonzalez M, Rusimbi C, Blears D,
            Khallou N, Han Z, Dirac-Svejstrup AB, Svejstrup JQ
  TITLE     PAF1C-mediated activation of CDK12/13 kinase activity is critical 
            for CTD phosphorylation and transcript elongation.
  JOURNAL   Mol Cell. 2025 May 15;85(10):1952-1967.e8. doi: 
            10.1016/j.molcel.2025.04.012. Epub 2025 May 1.
  PUBMED    40315851
REFERENCE   2  (bases 1 to 10154)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2025.04.012"; journalName: "Mol Cell"; date: 
            "2025-05-15- 15"; volume: "85"; issue: "10"; pages: "1952-1967.e8"
COMMENT     Sequence Label: pBIG1e-CDK12-KiD/Cyclin K
FEATURES             Location/Qualifiers
     source          1..10154
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(676..699)
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             complement(706..729)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     promoter        complement(775..866)
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     polyA_signal    961..1009
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     primer_bind     1034..1053
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     protein_bind    1099..1132
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        join(1528..1533,1534..1550,1551..1556)
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     CDS             1745..2278
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     mobile_element  2345..2569
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     primer_bind     complement(2703..2720)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2874..3462)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2954..2973)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3633..4424,4425..4493))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4256..4275
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4494..4598)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(4624..5078)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4761..4782)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     4972..4991
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     CDS             complement(5119..5910)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     mobile_element  6434..6599
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     polyA_signal    6914..7048
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        complement(8963..9054)
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     polyA_signal    9210..9344
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
ORIGIN
        1 aatacatcca cagctccaaa catctatggc tggtgtgtaa cgttcctctc ctagcagtag
       61 ttctggaggt cggtaccaca aagtaatgac tttgtttgtg taagggcgac tctcttcaga
      121 gttatagagc cgagcaagtc caaaatctgc tagtttgatt tgcccactgt tattcagcaa
      181 aatgttagaa cacttaatat cccgatgcag gaaattcttt ttgtgacagt attccaatcc
      241 ttccattagc tgtttcatga acgacttgat atggtcctca gaaaagtgca ccaaaccaga
      301 ttctagcagt cccattaagt catggtccat atactcaaat acaaggtaaa aggcaccttt
      361 gtccttcttg aaatccagtg catcttgttt atctgtgaca atttccttca tgttaacaac
      421 acttcggtgg attaactgac gaaggatttt gatttcacga atggctgtga ttgggaagcc
      481 ctctttctca ttgtctagtc tcaccttctt cagagccact agttctcctg tgtctttgtc
      541 cttggcttta tatacttggc cataggttcc ttctccaata atcccaataa tgtcaaactt
      601 gtccacacag cgtttccccc agtcgctttc tgtttgtctt ctttctccat aacgaggaca
      661 acaaattttt ggcatgggtc cctgaaagag gacttcaaga tcgagcttgt catcgtcgtc
      721 cttgtagtcc atggatccgc gcccgatggt gggacggtat gaataatccg gaatatttat
      781 aggttttttt attacaaaac tgttacgaaa acagtaaaat acttatttat ttgcgagatg
      841 gttatcattt taattatctc catgatctat taatattccg gagtaggtcg atttaaatct
      901 ttagaccata gagcgttcac ttgacagagt gccagccctg ggaccgaacc ccgcgtttat
      961 gaacaaacga cccaacaccc gtgcgtttta ttctgtcttt ttattgccgt catagcgcgg
     1021 gttccttccg gtattgtctc cttccgtgtt tcagttagcc tcgataaaaa ctaagctatg
     1081 tgaaccgttt aaacccagat aacttcgtat aatgtatgct atacgaagtt atggtaccgc
     1141 ggccgcgtag aggatctgtt gatcagcagt tcaacctgtt gatagtacaa acaaagctgt
     1201 agccaaccac tagaactata gctagagtcc tgggcgaaca aacgatgctc gccttccaga
     1261 aaaccgagga tgcgaaccac ttcatccggg gtcagcacca ccggcaagcg ccgcgacggc
     1321 cgaggtctac cgatctcctg aagcaagggc agatccgtgc acagcacctt gccgtagaag
     1381 aacagcaagg ccgccaatgc ctgacgatgc gtggagaccg aaaccttgcg ctcgttcgcc
     1441 agccaggaca gaaatgcctc gacttcgctg ctgcccaagg ttgccgggtg acgcacaccg
     1501 tggaaacgga tgaaggcaca aacccagttg acataagcct gttcggttcg taaactgtaa
     1561 tgcaagtagc gtatgcgctc acgcaactgg tccagaacct tgaccgaacg cagcggtggt
     1621 aacggcgcag tggcggtttt catggcttgt tatgactgtt tttttgtaca gtctatgcct
     1681 cgggcatcca agcagcaagc gcgttacgcc gtgggtcgat gtttgatgtt atggagcagc
     1741 aacgatgtta cgcagcagca acgatgttac gcagcagggc agtcgcccta aaacaaagtt
     1801 aggtggctca agtatgggca tcattcgcac atgtaggctc ggccctgacc aagtcaaatc
     1861 catgcgggct gctcttgatc ttttcggtcg tgagttcgga gacgtagcca cctactccca
     1921 acatcagccg gactccgatt acctcgggaa cttgctccgt agtaagacat tcatcgcgct
     1981 tgctgccttc gaccaagaag cggttgttgg cgctctcgcg gcttacgttc tgcccaagtt
     2041 tgagcagccg cgtagtgaga tctatatcta tgatctcgca gtctccggcg agcaccggag
     2101 gcagggcatt gccaccgcgc tcatcaatct cctcaagcat gaggccaacg cgcttggtgc
     2161 ttatgtgatc tacgtgcaag cagattacgg tgacgatccc gcagtggctc tctatacaaa
     2221 gttgggcata cgggaagaag tgatgcactt tgatatcgac ccaagtaccg ccacctaaca
     2281 attcgttcaa gccgagatcg gcttcccggc cgcggagttg ttcggtaaat tgtcacaacg
     2341 ccgcgaatat agtctttacc atgcccttgg ccacgcccct ctttaatacg acgggcaatt
     2401 tgcacttcag aaaatgaaga gtttgcttta gccataacaa aagtccagta tgctttttca
     2461 cagcataact ggactgattt cagtttacaa ctattctgtc tagtttaaga ctttattgtc
     2521 atagtttaga tctattttgt tcagtttaag actttattgt ccgcccacac ccgcttacgc
     2581 agggcatcca tttattactc aaccgtaacc gattttgcca ggttacgcgg ctggtctatg
     2641 cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc gcatcaggcg ctcttccgct
     2701 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac
     2761 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga
     2821 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat
     2881 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac
     2941 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
     3001 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
     3061 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg
     3121 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
     3181 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg
     3241 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac
     3301 ggctacacta gaagaatagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
     3361 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
     3421 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
     3481 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga
     3541 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc
     3601 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct
     3661 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata
     3721 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca
     3781 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga
     3841 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga
     3901 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg
     3961 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga
     4021 gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt
     4081 gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct
     4141 cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca
     4201 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat
     4261 accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga
     4321 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc
     4381 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg
     4441 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc
     4501 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt
     4561 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca
     4621 cctaaattgt aagcgttaat attttgttaa aattcgcgtt aaatttttgt taaatcagct
     4681 cattttttaa ccaataggcc gaaatcggca aaatccctta taaatcaaaa gaatagaccg
     4741 agatagggtt gagtgttgtt ccagtttgga acaagagtcc actattaaga acgtggactc
     4801 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
     4861 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
     4921 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
     4981 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
     5041 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccaa tccgtcggct
     5101 tgaacgaatt gttagacatt atttgccgac taccttggtg atctcgcctt tcacgtagtg
     5161 gacaaattct tccaactgat ctgcgcgcga ggccaagcga tcttcttctt gtccaagata
     5221 agcctgtcta gcttcaagta tgacgggctg atactgggcc ggcaggcgct ccattgccca
     5281 gtcggcagcg acatccttcg gcgcgatttt gccggttact gcgctgtacc aaatgcggga
     5341 caacgtaagc actacatttc gctcatcgcc agcccagtcg ggcggcgagt tccatagcgt
     5401 taaggtttca tttagcgcct caaatagatc ctgttcagga accggatcaa agagttcctc
     5461 cgccgctgga cctaccaagg caacgctatg ttctcttgct tttgtcagca agatagccag
     5521 atcaatgtcg atcgtggctg gctcgaagat acctgcaaga atgtcattgc gctgccattc
     5581 tccaaattgc agttcgcgct tagctggata acgccacgga atgatgtcgt cgtgcacaac
     5641 aatggtgact tctacagcgc ggagaatctc gctctctcca ggggaagccg aagtttccaa
     5701 aaggtcgttg atcaaagctc gccgcgttgt ttcatcaagc cttacggtca ccgtaaccag
     5761 caaatcaata tcactgtgtg gcttcaggcc gccatccact gcggagccgt acaaatgtac
     5821 ggccagcaac gtcggttcga gatggcgctc gatgacgcca actacctctg atagttgagt
     5881 cgatacttcg gcgatcaccg cttccctcat gatgtttaac tttgttttag ggcgactgcc
     5941 ctgctgcgta acatcgttgc tgctccataa catcaaacat cgacccacgg cgtaacgcgc
     6001 ttgctgcttg gatgcccgag gcatagactg taccccaaaa aaacagtcat aacaagccat
     6061 gaaaaccgcc actgcgccgt taccaccgct gcgttcggtc aaggttctgg accagttgcg
     6121 tgagcgcata cgctacttac cggtttattg actaccggaa gcagtgtgac cgtgtgcttc
     6181 tcaaatgcct gaggccagtt tgctcaggct ctccccgtgg aggtaataat tgacgatatg
     6241 atcatttatt ctgcctccca gttcaggctg caaataagcg ttgatattca gtcaattaca
     6301 aacattaata acgaagagat gacagaaaaa ttttcattct gtgacagaga aaaagtagcc
     6361 gaagatgacg gtttgtcaca tggagttggc aggatgtttg attaaaaaca taacaggaag
     6421 aaaaatgccc cgctgtgggc ggacaaaata gttgggaact gggaggggtg gaaatggagt
     6481 ttttaaggat tatttaggga agagtgacaa aatagatggg aactgggtgt agcgtcgtaa
     6541 gctaatacga aaattaaaaa tgacaaaata gtttggaact agatttcact tatctggttc
     6601 ggatctcctg tttaaaccaa gtcaatgtca gtgtttttaa ttaacccgta gtggctatgg
     6661 cagggcttgc cgccccgacg ttggctgcga gccctgggcc ttcacccgaa cttgggggtt
     6721 ggggtgggga aaaggaagaa acgcgggcgt attggtccca atggggtctc ggtggggtat
     6781 cgacgtaacc ccgattgaga tatagattta tttaaatggt tatgatagtt attgctcagc
     6841 ggtggcagca gccaactcag cttcctttcg ggctttgtta gcagccggat cttctaggct
     6901 caagcagtga tcagatccag acatgataag atacattgat gagtttggac aaaccacaac
     6961 tagaatgcag tgaaaaaaat gctttatttg tgaaatttgt gatgctattg ctttatttgt
     7021 aaccattata agctgcaata aacaagttaa caacaacaat tgcattcatt ttatgtttca
     7081 ggttcagggg gaggtgtggg aggtttttta aagcaagtaa aacctctaca aatgtggtat
     7141 ggctgattat gatcctctag tacttctcga caagctttta tctcatccag gctgcccgcc
     7201 ccagcccccc aactggaggc atgccgggtg ggggaatggg cgggggcaca ggaggctgtc
     7261 ctccaggggg gacggcagga ggtgggtagc tggctggcgg caggcccaac cctggaggag
     7321 gatgaggggg gactgcgtgg gtaggcggga ggcgtggggg tgggggtggg aagttggggt
     7381 tgtaggtggg tgggggtggg gggtagccag gagtaggggg tgggatggca ggtgggggga
     7441 aggaggctgg gggaggaggc acaggtggcg ggggcgggtt gggcgggtag acatgggggt
     7501 ggtagggcag gtgtgcaggt gggccgtagg cggggggcag ggcaccgtag gagggtccct
     7561 cggtcttcat catggactgc aggctctggt agccctctcc agacatgtag gagctggtgg
     7621 tggacatccc ggtcatgtag ctggaggggg gtgggggcgg gggccggtgt ggcagcggcg
     7681 gtggctggtg cacaggggcc gggtgggccg gagggggaat ctggactttg gggaggtcag
     7741 tggcatccac agggcccggc ggctcagcct cacctaaggc agcagcgagg ggaggcttcc
     7801 ggtctggagg tgggtgggct ggaggcaacg gtggatgagt ggtctcaatt ttggggattt
     7861 taggtggtgg tggttctgct gctttgttct cttctttggg agaaacaacc acggctcgct
     7921 taacctgtcg gggagaactg ggctgcggag agggtttctt gggctgttgg gctggctgct
     7981 gctgctgggc tggttgctgg gggtccttct gctggggctg ggatggttcg gagctttgag
     8041 acggctgtga ctgctgtact tgcggcactt gtggtgtagg ctgaagagat gggggctgtt
     8101 gcagctgatg gggggtgtga tgaggcatct gttgttttcc ttgtgagtaa agatccagga
     8161 tttggtggca gatgtcttcc aaaacgtcga ccgggacatc ttgaacaaac tgctcccacc
     8221 atctcctata catgggtttg gaggtccatt cttgtatttc aaatttgcac aaacgtcctg
     8281 cgagatacat cactgctact gctatgatct ctggttccca ctgcagtgac aaggtggtgc
     8341 agagactgtc atttacaaat gtccatgcca tttgaaccaa cttttgaatt ttgtttttat
     8401 cacctttgag ttgctttgca tattttagta ggaactggta tggatgttct acctgtaaat
     8461 caaacttgat ggtctgcagt aagattctct ccagaaccat tacttcctcc tttgggtcat
     8521 ctccaaactg gccaaattgt acatcattta ataaactacg agctgttttg atgatatctt
     8581 tacatttttt tggtgtttct tctactttcc cagccagaaa gaggcaacag gctcctgtca
     8641 catatcttgg gaattgcttg aaggaatgaa acatatagaa gcgatgaaaa taaattattc
     8701 cagttgccag ggtatcatag tgtagcccca aacgtgtgcc cacatcaaag atgaaccgag
     8761 cgccctctcg gcggtaccgg gcctcggtgg ctggatcaag tccttcaagt tgtgagggtg
     8821 tatgagccaa gtctttctta tcccagtacc aacatggctt tgtgtggtcc aggtttgctg
     8881 aagttactga agggcttgaa ttttctttat tctccttcat ggatccgcgc ccgatggtgg
     8941 gacggtatga ataatccgga atatttatag gtttttttat tacaaaactg ttacgaaaac
     9001 agtaaaatac ttatttattt gcgagatggt tatcatttta attatctcca tgatctatta
     9061 atattccgga gtaggtcgat ttaaacgtgc aatagtatcc agtttattta aatggttatg
     9121 atagttattg ctcagcggtg gcagcagcca actcagcttc ctttcgggct ttgttagcag
     9181 ccggatcttc taggctcaag cagtgatcag atccagacat gataagatac attgatgagt
     9241 ttggacaaac cacaactaga atgcagtgaa aaaaatgctt tatttgtgaa atttgtgatg
     9301 ctattgcttt atttgtaacc attataagct gcaataaaca agttaacaac aacaattgca
     9361 ttcattttat gtttcaggtt cagggggagg tgtgggaggt tttttaaagc aagtaaaacc
     9421 tctacaaatg tggtatggct gattatgatc ctctagtact tctcgacaag cttttagctg
     9481 ttcttcacag gctcagtact tctcgacaag cttttagctg ttcttcacag gctcagtact
     9541 tctcgacaag cttttagctg ttcttcacag gctcagtact tagtacttct cgacaagctt
     9601 ttagctgttc ttcacaggct cagtacttct cgacaagctt ttagctgttc ttcacaggct
     9661 cagtacttct cgacaagctt ttagctgttc ttcacaggct cagtacttgt ccctgaggta
     9721 gtttcttttc gagaagtttt ggatggaggt ggctcttcga ctacaacacc actttgtcgc
     9781 tgacgtcgcc gtttcttact ccacaactca tggcaatcct gccagtgggg gaggtctgga
     9841 ggagccattt tgctgagttc gacatcttta aggaagtcgc tctgtagggt ctgttcagct
     9901 gtgcaccgct tactaggatc tagtgtcagc atgtggtcca ataaatcaag tgctgcagaa
     9961 ggaatgaaag agaattcttc tcgtagacgc cttcgatatt gcttcttcgg tttcatggtg
    10021 ttgaagtagg gcagtttgat aacatcaggc cacacagctg gacaagggct accacaaagt
    10081 cggctgatca gttctagctg agccagttcc agattggctt gaaaaatagg cttctttgtg
    10141 aatagttccc caag
//
