LOCUS       sequence-46333-e         797 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  EXPRESs plasmid for Zebrafish Receptors encoding zebrafish unc5b 
            (netrin-1a) with rat CD4d3+4-bio-linker-His tag .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 797)
  AUTHORS   Sollner C, Wright GJ
  TITLE     A cell surface interaction network of neural leucine-rich repeat 
            receptors.
  JOURNAL   Genome Biol. 2009;10(9):R99. Epub 2009 Sep 18.
  PUBMED    19765300
REFERENCE   2  (bases 1 to 797)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genome 
            Biol."; date: "2009"; volume: "10(9)"; pages: "R99. Epub 2009 Sep 
            18"
COMMENT     Sequence Label: unc5bCD4d3+4-bio-linker-His
FEATURES             Location/Qualifiers
     source          1..797
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     216..235
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     polyA_signal    234..289
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     CDS             complement(341..358)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 actacagtca gagaacccct ttgtgtttgg tccccccccg tgtcacatgt ggaacagggc
       61 ccagttggca agttgtacca accaactgaa gggattacat gcactgcccc gcgaagaagg
      121 ggcagagatg tcgtagtcag gtttagttcg tccggggcgg ggatcgatcc tctagctaga
      181 gatctccgag ggatctcgac caaatgattt gccctcccat atgtccttcc gagtgagaga
      241 cacaaaaaat tccaacacac tattgcaatg aaaataaatt tcctttatta gccagaggtc
      301 gaggtcgggg gatccttaat taatcacgtg tctagactca atggtggtga tggtggtggg
      361 ggcccagagg ggccagagga ggcaggttcc tgtccctgtg gttccacagc atcttctggg
      421 cgtccaggat gtggtgcagg ctgccggaat tcaacccttt ggataaaacc tggatcttgg
      481 agtccatctt gacctcttca ccttcactca gtagacattg ccacacccct gcttcagggg
      541 cctgcacttg aatcactttc tcctgcctgg agaccctggc ctcctgattc tcctgcttca
      601 agatcagtct catcttgggt gaggtgggtc ccatcacctc acaggtcaaa gtgttgctgt
      661 cgggctgagt cactttcatc accaccaggt tcacttcctg atacagtatc cctctgtcca
      721 gagtcagggt caggttgcca gaaccagcaa actgaaggga gacctggggt atctgaaggg
      781 tgagtgggag cgtttcg
//
