LOCUS       sequence-46335-e         776 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  A positive control receptor bait with His-tag (rat CD4d3+4-HIS) and 
            prey (rat CD200) EXPRESs plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 776)
  AUTHORS   Bushell KM, Sollner C, Schuster-Boeckler B, Bateman A, Wright GJ
  TITLE     Large-scale screening for novel low-affinity extracellular protein 
            interactions.
  JOURNAL   Genome Res. 2008 Apr;18(4):622-30. Epub 2008 Feb 22.
  PUBMED    18296487
REFERENCE   2  (bases 1 to 776)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genome 
            Res."; date: "2008-04"; volume: "18(4)"; pages: "622-30. Epub 2008 
            Feb 22"
COMMENT     Sequence Label: CD200CD4d3+4-His
FEATURES             Location/Qualifiers
     source          1..776
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     216..235
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     polyA_signal    234..289
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     CDS             complement(341..358)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 actacagtca gagaacccct ttgtgtttgg tccccccccg tgtcacatgt ggaacagggc
       61 ccagttggca agttgtacca accaactgaa gggattacat gcactgcccc gcgaagaagg
      121 ggcagagatg tcgtagtcag gtttagttcg tccggggcgg ggatcgatcc tctagctaga
      181 gatctccgag ggatctcgac caaatgattt gccctcccat atgtccttcc gagtgagaga
      241 cacaaaaaat tccaacacac tattgcaatg aaaataaatt tcctttatta gccagaggtc
      301 gaggtcgggg gatccttaat taatcacgtg tctagactta gtggtgatgg tgatgatgtg
      361 gtccggaatt caaccctttg gataaaacct ggatcttgga gtccatcttg acctcttcac
      421 cttcactcag tagacattgc cacacccctg cttcaggggc ctgcacttga atcactttct
      481 cctgcctgga gaccctggcc tcctgattct cctgcttcaa gatcagtctc atcttgggtg
      541 aggtgggtcc catcacctca caggtcaaag tgttgctgtc gggctgagtc actttcatca
      601 ccaccaggtt cacttcctga tacagtatcc ctctgtccag agtcagggtc aggttgccag
      661 aaccagcaaa ctgaagggag acctggggta tctgaagggt gagtgggagc gtttcggaca
      721 gctggaactt ggggttgcta gtagacttct gcacagacac cttttggttc tttagg
//
