LOCUS       sequence-46336-e         824 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  A positive control receptor bait with bio-linker-His (rat 
            CD4d3+4-bio-linker-his) and prey (rat CD200 receptor) EXPRESs 
            plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 824)
  AUTHORS   Bushell KM, Sollner C, Schuster-Boeckler B, Bateman A, Wright GJ
  TITLE     Large-scale screening for novel low-affinity extracellular protein 
            interactions.
  JOURNAL   Genome Res. 2008 Apr;18(4):622-30. Epub 2008 Feb 22.
  PUBMED    18296487
REFERENCE   2  (bases 1 to 824)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genome 
            Res."; date: "2008-04"; volume: "18(4)"; pages: "622-30. Epub 2008 
            Feb 22"
COMMENT     Sequence Label: CD200RCD4d3+4-bio-linker-His
FEATURES             Location/Qualifiers
     source          1..824
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     216..235
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     polyA_signal    234..289
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     CDS             complement(341..358)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 actacagtca gagaacccct ttgtgtttgg tccccccccg tgtcacatgt ggaacagggc
       61 ccagttggca agttgtacca accaactgaa gggattacat gcactgcccc gcgaagaagg
      121 ggcagagatg tcgtagtcag gtttagttcg tccggggcgg ggatcgatcc tctagctaga
      181 gatctccgag ggatctcgac caaatgattt gccctcccat atgtccttcc gagtgagaga
      241 cacaaaaaat tccaacacac tattgcaatg aaaataaatt tcctttatta gccagaggtc
      301 gaggtcgggg gatccttaat taatcacgtg tctagactca atggtggtga tggtggtggg
      361 ggcccagagg ggccagagga ggcaggttcc tgtccctgtg gttccacagc atcttctggg
      421 cgtccaggat gtggtgcagg ctgccggaat tcaacccttt ggataaaacc tggatcttgg
      481 agtccatctt gacctcttca ccttcactca gtagacattg ccacacccct gcttcagggg
      541 cctgcacttg aatcactttc tcctgcctgg agaccctggc ctcctgattc tcctgcttca
      601 agatcagtct catcttgggt gaggtgggtc ccatcacctc acaggtcaaa gtgttgctgt
      661 cgggctgagt cactttcatc accaccaggt tcacttcctg atacagtatc cctctgtcca
      721 gagtcagggt caggttgcca gaaccagcaa actgaaggga gacctggggt atctgaaggg
      781 tgagtgggag cgtttcggac agctggaact tggggttgct agta
//
