LOCUS       sequence-46339-e         795 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 795)
  AUTHORS   Ferguson SM, Brasnjo G, Hayashi M, Wolfel M, Collesi C, Giovedi S, 
            Raimondi A, Gong LW, Ariel P, Paradise S, O'toole E, Flavell R, 
            Cremona O, Miesenbock G, Ryan TA, De Camilli P
  TITLE     A selective activity-dependent requirement for dynamin 1 in synaptic
            vesicle endocytosis.
  JOURNAL   Science. 2007 Apr 27;316(5824):570-4.
  PUBMED    17463283
REFERENCE   2  (bases 1 to 795)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "2007-04-27"; volume: "316(5824)"; pages: "570-4"
COMMENT     Sequence Label: HA-mDyn1 pcDNA3
FEATURES             Location/Qualifiers
     source          1..795
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     50..69
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        50..68
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      88..97
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             100..126
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     RBS             705..713
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
ORIGIN
        1 gagctctctg gctaactaga gaacccactg cttactggct tatcgaaatt aatacgactc
       61 actataggga gacccaagct tggtaccgcc accatggcgt acccatacga cgtcccagac
      121 tacgcgggat ccgaattcgg caaccgcggc atggaagacc tcatcccgct ggttaaccgg
      181 ttacaggacg ccttctccgc catcggccag aacgcggacc tcgacctgcc gcagatcgcc
      241 gtggtaggcg gccagagcgc cggcaagagc tcggtgctgg agaatttcgt gggcagggac
      301 ttcttgcccc gaggatctgg catcgtcacc cggcgtcccc tggtcctgca gctggttaat
      361 tctaccacag aatatgccga gttcctgcac tgcaagggga agaaattcac cgacttcgag
      421 gaggtgcgcc tggagatcga ggctgagacc gatcgagtca ccggcaccaa caagggcatt
      481 tcgccagtgc ccatcaacct gcgggtctac tcgccccatg tgctgaacct gactctagtg
      541 gacctgccag gaatgaccaa ggtcccagtt ggggaccaac ctcctgatat cgagttccag
      601 atccgggaca tgcttatgca gttcgtcact aaggagaact gccttatcct ggctgtgtcc
      661 cctgccaact cggatttggc caactctgat gccctcaaga tcgctaagga ggtggacccc
      721 cagggtcagc gcaccattgg ggtcatcacc aagttggacc tgatggacga gggcacagat
      781 gcgcgggacg tgcta
//
