LOCUS       sequence-46340-e         861 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 861)
  AUTHORS   Ferguson SM, Brasnjo G, Hayashi M, Wolfel M, Collesi C, Giovedi S, 
            Raimondi A, Gong LW, Ariel P, Paradise S, O'toole E, Flavell R, 
            Cremona O, Miesenbock G, Ryan TA, De Camilli P
  TITLE     A selective activity-dependent requirement for dynamin 1 in synaptic
            vesicle endocytosis.
  JOURNAL   Science. 2007 Apr 27;316(5824):570-4.
  PUBMED    17463283
REFERENCE   2  (bases 1 to 861)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "2007-04-27"; volume: "316(5824)"; pages: "570-4"
COMMENT     Sequence Label: HA-mDyn2 pcDNA3
FEATURES             Location/Qualifiers
     source          1..861
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     50..69
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        50..68
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      88..97
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             100..126
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 gagctctctg gctaactaga gaacccactg cttactggct tatcgaaatt aatacgactc
       61 actataggga gacccaagct tggtaccgcc accatggcgt acccatacga cgtcccagac
      121 tacgcgggat ccgaattcgg caaccgcggg atggaagagc tcatcccgct ggttaacaag
      181 ctgcaggacg ccttcagctc catcggtcag agctgccacc tggacctgcc gcagatcgcc
      241 gtggtgggcg gccagagcgc cggcaaaagt tcggtgctcg agaacttcgt gggccgggac
      301 ttccttccac gaggatcagg aattgtcacc cggaggcctc tcattctgca gctcatcttt
      361 tccaaaacag aatatgcgga atttttgcac tgcaagtcca aaaaatttac agactttgat
      421 gaagtccggc aggagatcga agcagagact gaccgggtca caggcaccaa caaaggcatc
      481 tcccctgtgc ccatcaacct tcgggtctac tcaccacacg tgttgaactt gaccctcatc
      541 gacctcccag gcatcactaa ggtgccggtg ggggaccagc cgccagacat cgagtaccag
      601 atcaaggaca tgatcctgca gttcatcagc cgcgagagca gcctcattct tgccgtcaca
      661 cctgccaaca tggacttggc caactcagac gccctcaagc tggccaagga ggtggacccc
      721 caaggcctac ggaccatcgg tgtcatcacc aagctagact tgatggatga aggcacagac
      781 gccagggatg tcctggaaaa caagctactg cccttgagaa gaggctatat cggcgtggtt
      841 aaccgaagcc agaaagacat c
//
