LOCUS       sequence-46341-e         911 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 911)
  AUTHORS   Ferguson SM, Brasnjo G, Hayashi M, Wolfel M, Collesi C, Giovedi S, 
            Raimondi A, Gong LW, Ariel P, Paradise S, O'toole E, Flavell R, 
            Cremona O, Miesenbock G, Ryan TA, De Camilli P
  TITLE     A selective activity-dependent requirement for dynamin 1 in synaptic
            vesicle endocytosis.
  JOURNAL   Science. 2007 Apr 27;316(5824):570-4.
  PUBMED    17463283
REFERENCE   2  (bases 1 to 911)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "2007-04-27"; volume: "316(5824)"; pages: "570-4"
COMMENT     Sequence Label: HA-mDyn3 pcDNA3
FEATURES             Location/Qualifiers
     source          1..911
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     50..69
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        50..68
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      88..97
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             100..126
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 gagctctctg gctaactaga gaacccactg cttactggct tatcgaaatt aatacgactc
       61 actataggga gacccaagct tggtaccgcc accatggcgt acccatacga cgtcccagac
      121 tacgcgggat ccgaattcgg gaaccgggag atggaagagc tgatccctct ggtgaaccgt
      181 ctgcaggacg ctttctcggc tctgggacag agctgtctgc tagagctgcc acagatcgcc
      241 gtggtgggcg gccagagcgc cggcaagagc tcagtgctcg agaatttcgt gggcagggac
      301 tttcttcctc gaggctcagg cattgtaacc agacggcctc ttgtactcca gcttgtcacc
      361 tctaaagcag aatatgccga gtttctacat tgcaaaggaa aaaaatttac agattttgat
      421 gaagttcgac atgagatcga agcagaaaca gaccgcgtaa caggaatgaa taaaggcatc
      481 tcttctatac caattaacct ccgagtctac tctccccacg tgttaaatct aacgctaatc
      541 gacctacctg gaataacgaa agtgcccgtg ggagaccagc caccagatat cgagtatcag
      601 atcagagata tgattatgca gttcatcacg agggagaact gtctgatttt ggctgtcacg
      661 ccagccaaca ccgatcttgc gaactcggat gcactgaagc tagccaaaga agttgatcct
      721 caaggtctga ggaccattgg cgtgatcacc aagctggatc tcatggatga aggaacagat
      781 gccagggatg tgctggagaa caagctgctg cctcttcgca ggggctacgt tggggtggtc
      841 aacagaagcc agaaggacat agatggaaag aaagacatta aggcagccat gttggcagaa
      901 aggaaatttt t
//
