LOCUS       sequence-46346-e         898 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  EXPRESs plasmid for Zebrafish Receptors encoding zebrafish unc5b 
            (netrin-1a) with rat CD4d3+4-bio-linker-His tag .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 898)
  AUTHORS   Sollner C, Wright GJ
  TITLE     A cell surface interaction network of neural leucine-rich repeat 
            receptors.
  JOURNAL   Genome Biol. 2009;10(9):R99. Epub 2009 Sep 18.
  PUBMED    19765300
REFERENCE   2  (bases 1 to 898)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genome 
            Biol."; date: "2009"; volume: "10(9)"; pages: "R99. Epub 2009 Sep 
            18"
COMMENT     Sequence Label: unc5bCD4d3+4-bio-linker-His
FEATURES             Location/Qualifiers
     source          1..898
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     6..30
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     511..530
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      564..573
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 agcagagctc gtttagtgaa ccgtcagatc ctcactctct tccgcatcgc tgtctgcgag
       61 ggccagctgt tgggctcgcg gttgaggaca aactcttcgc ggtctttcca gtactcttgg
      121 atcggaaacc cgtcggcctc cgaacggtac tccgccaccg agggacctga gcgagtccgc
      181 atcgaccgga tcggaaaacc tctcgagaaa ggcgtctaac cagtcacagt cgcaaggtag
      241 gctgagcacc gtggcgggcg gcagcgggtg gcggtcgggg ttgtttctgg cggaggtgct
      301 gctgatgatg taattaaagt aggcggtctt gagacggcgg atggtcgagg tgaggtgtgg
      361 caggcttgag atccagctgt tggggtgagt actccctctc aaaagcgggc attacttctg
      421 cgctaagatt gtcagtttcc aaaaacgagg aggatttgat attcacctgg cccgatctgg
      481 ccatacactt gagtgacaat gacatccact ttgcctttct ctccacaggt gtccactccc
      541 aggtccaagt ttaaactgcg gccgccacca tggtctcaac gtgtatacac cgcgaccagt
      601 ctcccgcctc cctgctcggt ttacttctac tcagctcaac cttagtgata catgcagatg
      661 gcagcgatta cagtgaagtt ctcccggact cgtttccatc agctcctgcc gaaccgctgc
      721 cagaattcca gagtgagccc gaggatgcat tcatagtaaa aaacagaccc gtcaaactgt
      781 cctgcaaggc cgcccctgcc acacagatat actttaaatg caatggagaa tgggtgaacc
      841 agaatgacca tgtgaccaag gaaagtctgg accatatcac aggtctggta gtgagaga
//
