LOCUS       sequence-463529-        3740 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express the short NSP5 protein (17 amino acids) .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3740)
  TITLE     Rotavirus Nonstructural Protein (NSP) plasmids
REFERENCE   2  (bases 1 to 3740)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pT7-NSP5(Y18Stop)SA11
FEATURES             Location/Qualifiers
     source          1..3740
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     233..250
                     /label=L4440
                     /note="L4440 vector, forward primer"
     promoter        334..352
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             complement(398..409)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(1164..1182)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      1178..1225
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        complement(1293..1311)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(1294..1311)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(1601..1620)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(1830..1849)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     1949..1971
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(2009..2027)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        2095..2199
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2200..2268,2269..3060)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2418..2437)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      join(3231..3740,1..79)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3720..3739
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
       61 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
      121 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat
      181 taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc
      241 agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcattggcc
      301 gattcattaa tgcaggggga tatcgatccc ggttaatacg actcactata ggcttttaaa
      361 gcgctacagt gatgtctctc agtattgacg tgacgagtct tccttctatt ccttcaacta
      421 tatagaagaa tgaatcgtct tcaacaacgt caactctttc tggaaaatct attggtagga
      481 gtgaacagta catttcacca gatgcagaag cattcaataa atacatgctg tcgaagtctc
      541 cagaggatat tggaccatct gattctgctt caaacgatcc actcaccagt ttttcgatta
      601 gatcgaatgc agttaagaca aacgcagacg ctggcgtgtc tatggattca tcagcacaat
      661 cacgaccttc aagtaatgtc ggatgcgatc aagtggattt ctccttaaat aaaggcttaa
      721 aagtaaaagc taatttggac tcatcaatat caatatctac ggatactaaa aaggagaaat
      781 caaaacaaaa ccataaaagt aggaagcact acccaagaat tgaagcagag tctgattcag
      841 atgattatgt actggatgat tcagatagtg atgatggtaa atgtaagaac tgtaaatata
      901 agaagaaata cttcgcatta agaatgagaa tgaaacaagt cgcaatgcaa ttgattgaag
      961 atttgtaagt ctgacctggg aacacactag ggagctcccc actcccgttt tgtgaccggg
     1021 tcggcatggc atctccacct cctcgcggtc cgacctgggc atccgaagga ggacgtcgtc
     1081 cactcggatg gctaagggag agctcaaaaa aaaggatccg gctgctaaca aagcccgaaa
     1141 ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc
     1201 taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat cgagatcctc
     1261 tagagtcgac ctgcaggcat gcaagcttgt attctatagt gtcacctaaa tcgtatgtgt
     1321 atgatacata aggttatgta ttaattgtag ccgcgttcta acgacaatat gtacaagcct
     1381 aattgtgtag catctggctt actgaagcag accctatcat ctctctcgta aactgccgtc
     1441 agagtcggtt tggttggacg aaccttctga gtttctggta acgccgtccc gcacccggaa
     1501 atggtcagcg aaccaatcag cagggtcatc gctagccaga tcctctacgc cggacgcatc
     1561 gtggccggca tcaccggcgc cacaggtgcg gttgctggcg cctatatcgc cgacatcacc
     1621 gatggggaag atcgggctcg ccacttcggg ctcatgagcg cttgtttcgg cgtgggtatg
     1681 gtggcaggcc ccgtggccgg gggactgttg ggcgccatct ccttgcacca ttccttgcgg
     1741 cggcggtgct caacggcctc aacctactac tgggctgctt cttaatgcag gagtcgcata
     1801 agggagagcg tcgaatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc
     1861 cagccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca
     1921 tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg
     1981 tcatcaccga aacgcgcgag acgaaagggc ctcgtgatac gcctattttt ataggttaat
     2041 gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga
     2101 acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa
     2161 ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt
     2221 gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg
     2281 ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg
     2341 gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg
     2401 agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag
     2461 caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca
     2521 gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg
     2581 agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc
     2641 gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg
     2701 aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg
     2761 ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac
     2821 tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg
     2881 tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg
     2941 gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact
     3001 atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa
     3061 ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt
     3121 aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag
     3181 ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct
     3241 ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt
     3301 tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg
     3361 cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct
     3421 gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc
     3481 gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg
     3541 tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa
     3601 ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg
     3661 gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg
     3721 ggaaacgcct ggtatcttta
//
