LOCUS       sequence-463541-       10472 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  C-to-T base editor.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10472)
  AUTHORS   Matuszek Z, Arbab M, Kesavan M, Hsu A, Roy JCL, Zhao J, Yu T, 
            Weisburd B, Newby GA, Doherty NJ, Wu M, Shibata S, Cristian A, Tao 
            YA, Fearnley LG, Bahlo M, Rehm HL, Xie J, Gao G, Mouro Pinto R, Liu 
            DR
  TITLE     Base editing of trinucleotide repeats that cause Huntington's 
            disease and Friedreich's ataxia reduces somatic repeat expansions in
            patient cells and in mice.
  JOURNAL   Nat Genet. 2025 May 26. doi: 10.1038/s41588-025-02172-8.
  PUBMED    40419681
REFERENCE   2  (bases 1 to 10472)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Genet. 
            2025 May 26. doi: 10.1038/s41588-025-02172-8."
COMMENT     Sequence Label: p2T-CMV-EA-evoAPOBEC1max_NG-BlastR
FEATURES             Location/Qualifiers
     source          1..10472
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             3117..3365
                     /codon_start=1
                     /product="uracil-DNA glycosylase inhibitor from a Bacillus 
                     subtilis bacteriophage (Mol et al., 1995)"
                     /label=UGI
                     /translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTA
                     YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
     CDS             3396..3644
                     /codon_start=1
                     /product="uracil-DNA glycosylase inhibitor from a Bacillus 
                     subtilis bacteriophage (Mol et al., 1995)"
                     /label=UGI
                     /translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTA
                     YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
     CDS             3687..3707
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             3716..3733
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(3756..3773)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    3762..3986
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(4057..4073)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4057..4073)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4070..4092)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4081..4097
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4105..4111,4112..4129,4130..4135))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4150..4171
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     polyA_signal    4288..4409
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4299..4318)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     4353..4372
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(4567..4965)
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     promoter        complement(4984..5031)
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     promoter        complement(5079..5408)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5093..5228
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(5147..5166)
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     5393..5413
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     misc_feature    join(5532..5719,5720..5731)
                     /label=Tol2 left end
                     /note="left end of the Tol2 transposon from the medaka fish
                     Oryzias latipes (Kawakami, 2007)"
     rep_origin      complement(5792..6380)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5872..5891)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6551..7342,7343..7411))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7174..7193
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7412..7516)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(7595..7614)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     misc_feature    join(7806..7817,7818..7955)
                     /label=Tol2 right end
                     /note="right end of the Tol2 transposon from the medaka 
                     fish Oryzias latipes (Kawakami, 2007)"
     enhancer        7964..8343
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        8344..8547
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     8498..8518
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     8589..8608
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        8589..8607
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             8657..8677
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             join(9458..10472,1..3086)
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SIRPKRNSDKLIARKKDWDPKKYGGFVSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASARFLQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPRAFKYFDTTIDRKVYRSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
ORIGIN
        1 ggcagcagct gcctgagaag tacaaagaga ttttcttcga ccagagcaag aacggctacg
       61 ccggctacat tgacggcgga gccagccagg aagagttcta caagttcatc aagcccatcc
      121 tggaaaagat ggacggcacc gaggaactgc tcgtgaagct gaacagagag gacctgctgc
      181 ggaagcagcg gaccttcgac aacggcagca tcccccacca gatccacctg ggagagctgc
      241 acgccattct gcggcggcag gaagattttt acccattcct gaaggacaac cgggaaaaga
      301 tcgagaagat cctgaccttc cgcatcccct actacgtggg ccctctggcc aggggaaaca
      361 gcagattcgc ctggatgacc agaaagagcg aggaaaccat caccccctgg aacttcgagg
      421 aagtggtgga caagggcgct tccgcccaga gcttcatcga gcggatgacc aacttcgata
      481 agaacctgcc caacgagaag gtgctgccca agcacagcct gctgtacgag tacttcaccg
      541 tgtataacga gctgaccaaa gtgaaatacg tgaccgaggg aatgagaaag cccgccttcc
      601 tgagcggcga gcagaaaaag gccatcgtgg acctgctgtt caagaccaac cggaaagtga
      661 ccgtgaagca gctgaaagag gactacttca agaaaatcga gtgcttcgac tccgtggaaa
      721 tctccggcgt ggaagatcgg ttcaacgcct ccctgggcac ataccacgat ctgctgaaaa
      781 ttatcaagga caaggacttc ctggacaatg aggaaaacga ggacattctg gaagatatcg
      841 tgctgaccct gacactgttt gaggacagag agatgatcga ggaacggctg aaaacctatg
      901 cccacctgtt cgacgacaaa gtgatgaagc agctgaagcg gcggagatac accggctggg
      961 gcaggctgag ccggaagctg atcaacggca tccgggacaa gcagtccggc aagacaatcc
     1021 tggatttcct gaagtccgac ggcttcgcca acagaaactt catgcagctg atccacgacg
     1081 acagcctgac ctttaaagag gacatccaga aagcccaggt gtccggccag ggcgatagcc
     1141 tgcacgagca cattgccaat ctggccggca gccccgccat taagaagggc atcctgcaga
     1201 cagtgaaggt ggtggacgag ctcgtgaaag tgatgggccg gcacaagccc gagaacatcg
     1261 tgatcgaaat ggccagagag aaccagacca cccagaaggg acagaagaac agccgcgaga
     1321 gaatgaagcg gatcgaagag ggcatcaaag agctgggcag ccagatcctg aaagaacacc
     1381 ccgtggaaaa cacccagctg cagaacgaga agctgtacct gtactacctg cagaatgggc
     1441 gggatatgta cgtggaccag gaactggaca tcaaccggct gtccgactac gatgtggacc
     1501 atatcgtgcc tcagagcttt ctgaaggacg actccatcga caacaaggtg ctgaccagaa
     1561 gcgacaagaa ccggggcaag agcgacaacg tgccctccga agaggtcgtg aagaagatga
     1621 agaactactg gcggcagctg ctgaacgcca agctgattac ccagagaaag ttcgacaatc
     1681 tgaccaaggc cgagagaggc ggcctgagcg aactggataa ggccggcttc atcaagagac
     1741 agctggtgga aacccggcag atcacaaagc acgtggcaca gatcctggac tcccggatga
     1801 acactaagta cgacgagaat gacaagctga tccgggaagt gaaagtgatc accctgaagt
     1861 ccaagctggt gtccgatttc cggaaggatt tccagtttta caaagtgcgc gagatcaaca
     1921 actaccacca cgcccacgac gcctacctga acgccgtcgt gggaaccgcc ctgatcaaaa
     1981 agtaccctaa gctggaaagc gagttcgtgt acggcgacta caaggtgtac gacgtgcgga
     2041 agatgatcgc caagagcgag caggaaatcg gcaaggctac cgccaagtac ttcttctaca
     2101 gcaacatcat gaactttttc aagaccgaga ttaccctggc caacggcgag atccggaagc
     2161 ggcctctgat cgagacaaac ggcgaaaccg gggagatcgt gtgggataag ggccgggatt
     2221 ttgccaccgt gcggaaagtg ctgagcatgc cccaagtgaa tatcgtgaaa aagaccgagg
     2281 tgcagacagg cggcttcagc aaagagtcta tccggcccaa gaggaacagc gataagctga
     2341 tcgccagaaa gaaggactgg gaccctaaga agtacggcgg cttcgtcagc cccaccgtgg
     2401 cctattctgt gctggtggtg gccaaagtgg aaaagggcaa gtccaagaaa ctgaagagtg
     2461 tgaaagagct gctggggatc accatcatgg aaagaagcag cttcgagaag aatcccatcg
     2521 actttctgga agccaagggc tacaaagaag tgaaaaagga cctgatcatc aagctgccta
     2581 agtactccct gttcgagctg gaaaacggcc ggaagagaat gctggcctct gcccgcttcc
     2641 tgcagaaggg aaacgaactg gccctgccct ccaaatatgt gaacttcctg tacctggcca
     2701 gccactatga gaagctgaag ggctcccccg aggataatga gcagaaacag ctgtttgtgg
     2761 aacagcacaa gcactacctg gacgagatca tcgagcagat cagcgagttc tccaagagag
     2821 tgatcctggc cgacgctaat ctggacaaag tgctgtccgc ctacaacaag caccgggata
     2881 agcccatcag agagcaggcc gagaatatca tccacctgtt taccctgacc aatctgggag
     2941 cccctcgcgc cttcaagtac tttgacacca ccatcgaccg gaaggtgtac cgcagcacca
     3001 aagaggtgct ggacgccacc ctgatccacc agagcatcac cggcctgtac gagacacgga
     3061 tcgacctgtc tcagctggga ggtgacagcg gcgggagcgg cgggagcggg gggagcacta
     3121 atctgagcga catcattgag aaggagactg ggaaacagct ggtcattcag gagtccatcc
     3181 tgatgctgcc tgaggaggtg gaggaagtga tcggcaacaa gccagagtct gacatcctgg
     3241 tgcacaccgc ctacgacgag tccacagatg agaatgtgat gctgctgacc tctgacgccc
     3301 ccgagtataa gccttgggcc ctggtcatcc aggattctaa cggcgagaat aagatcaaga
     3361 tgctgagcgg aggatccgga ggatctggag gcagcaccaa cctgtctgac atcatcgaga
     3421 aggagacagg caagcagctg gtcatccagg agagcatcct gatgctgccc gaagaagtcg
     3481 aagaagtgat cggaaacaag cctgagagcg atatcctggt ccataccgcc tacgacgaga
     3541 gtaccgacga aaatgtgatg ctgctgacat ccgacgcccc agagtataag ccctgggctc
     3601 tggtcatcca ggattccaac ggagagaaca aaatcaaaat gctgtctggc ggctcaaaaa
     3661 gaaccgccga cggcagcgaa ttcgagccca agaagaagag gaaagtctaa ccggtcatca
     3721 tcaccatcac cattgagttt aaacccgctg atcagcctcg actgtgcctt ctagttgcca
     3781 gccatctgtt gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg ccactcccac
     3841 tgtcctttcc taataaaatg aggaaattgc atcgcattgt ctgagtaggt gtcattctat
     3901 tctggggggt ggggtggggc aggacagcaa gggggaggat tgggaagaca atagcaggca
     3961 tgctggggat gcggtgggct ctatggcttc tgaggcggaa agaaccagct ggggctcgat
     4021 accgtcgacc tctagctaga gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa
     4081 ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagccta
     4141 gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca
     4201 gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg
     4261 tttgcgtatt gggcgctaca gacatgataa gatacattga tgagtttgga caaaccacaa
     4321 ctagaatgca gtgaaaaaaa tgctttattt gtgaaatttg tgatgctatt gctttatttg
     4381 taaccattat aagctgcaat aaacaagttg gggtgggcga agaactccag catgagatcc
     4441 ccgcgctgga ggatcatcca gccggcgtcc cggaaaacga ttccgaagcc caacctttca
     4501 tagaaggcgg cggtggaatc gaaatctcgt agcacgtgtc agtcctgctc ctcggccacg
     4561 aagtgcttag ccctcccaca cataaccaga gggcagcaat tcacgaatcc caactgccgt
     4621 cggctgtcca tcactgtcct tcactatggc tttgatccca ggatgcagat cgagaagcac
     4681 ctgtcggcac cgtccgcagg ggctcaagat gcccctgttc tcatttccga tcgcgacgat
     4741 acaagtcagg ttgccagctg ccgcagcagc agcagtgccc agcaccacga gttctgcaca
     4801 aggtccccca gtaaaatgat atacattgac accagtgaag atgcggccgt cgctagagag
     4861 agctgcgctg gcgacgctgt agtcttcaga gatggggatg ctgttgattg tagccgttgc
     4921 tctttcaatg agggtggatt cttcttgaga caaaggcttg gccatggttt agttcctcac
     4981 cttgtcgtat tatactatgc cgatatacta tgccgatgat taattgtcaa cacgtgctga
     5041 tcagatccga aaatggatat acaagctccc gggagctttt tgcaaaagcc taggcctcca
     5101 aaaaagcctc ctcactactt ctggaatagc tcagaggcag aggcggcctc ggcctctgca
     5161 taaataaaaa aaattagtca gccatggggc ggagaatggg cggaactggg cggagttaga
     5221 ggcgggatgg gcggagttag gggcgggact atggttgctg actaattgag atgcatgctt
     5281 tgcatacttc tgcctgctgg ggagcctggg gactttccac acctggttgc tgactaattg
     5341 agatgcatgc tttgcatact tctgcctgct ggggagcctg gggactttcc acaccctaac
     5401 tgacacacat tccacagaat tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc
     5461 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg
     5521 aaagaacatg taagtgatct ccaaaaaata agtacttttt gactgtaaat aaaattgtaa
     5581 ggagtaaaaa gtactttttt ttctaaaaaa atgtaattaa gtaaaagtaa aagtattgat
     5641 ttttaattgt actcaagtaa agtaaaaatc cccaaaaata atacttaagt acagtaatca
     5701 agtaaaatta ctcaagtact ttacacctct gcatgtgagc aaaaggccag caaaaggcca
     5761 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc
     5821 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc
     5881 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg
     5941 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta
     6001 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg
     6061 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac
     6121 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag
     6181 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat
     6241 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat
     6301 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc
     6361 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacactcagt
     6421 ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct
     6481 agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt
     6541 ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc
     6601 gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac
     6661 catctggccc cagtgctgca atgataccgc gggacccacg ctcaccggct ccagatttat
     6721 cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg
     6781 cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata
     6841 gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta
     6901 tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt
     6961 gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag
     7021 tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa
     7081 gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc
     7141 gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt
     7201 taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc
     7261 tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta
     7321 ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa
     7381 taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca
     7441 tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac
     7501 aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtcgac ggatcgggag
     7561 atcgatctcc cgatccccta aggtcgactc tcagtacaat ctgctctgat gccgcatagt
     7621 taagccagta tctgctccct gcttgtgtgt tggaggtcgc tgagtagtgc gcgagcaaaa
     7681 tttaagctac aacaaggcaa ggcttgaccg acaattgcat gaagaatctg cttagggtta
     7741 ggcgttttgc gctgcttcgc gatgtacggg ccagatatac gcgggtaccg gcatatggtt
     7801 cttgacagag gtgtaaaaag tactcaaaaa ttttactcaa gtgaaagtac aagtacttag
     7861 ggaaaatttt actcaattaa aagtaaaagt atctggctag aatcttactt gagtaaaagt
     7921 aaaaaagtac tccattaaaa ttgtacttga gtattaacgc gttgacattg attattgact
     7981 agttattaat agtaatcaat tacggggtca ttagttcata gcccatatat ggagttccgc
     8041 gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg
     8101 acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa
     8161 tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca
     8221 agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac
     8281 atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc
     8341 atggtgatgc ggttttggca gtacatcaat gggcgtggat agcggtttga ctcacgggga
     8401 tttccaagtc tccaccccat tgacgtcaat gggagtttgt tttggcacca aaatcaacgg
     8461 gactttccaa aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg taggcgtgta
     8521 cggtgggagg tctatataag cagagctggt ttagtgaacc gtcagatccg ctagagatcc
     8581 gcggccgcta atacgactca ctatagggag agccgccacc atgaaacgga cagccgacgg
     8641 aagcgagttc gagtcaccaa agaagaagcg gaaagtcagt tcaaagactg ggcctgtcgc
     8701 cgtcgatcca accctgcgcc gccggattga acctcacgag tttgaagtgt tctttgaccc
     8761 ccgggagctg agaaaggaga catgcctgct gtacgagatc aactggggag gcagggaggc
     8821 catctggagg cacacctctc agaacacaaa taagcacgtg gaggtgaact tcatcgagaa
     8881 gtttaccaca gagcggtact tctgccccaa taccagatgt agcatcacat ggtttctgag
     8941 ctggtcccct tgcggagagt gtagcagggc catcaccgag ttcctgtcca gatatccaaa
     9001 tgtgacactg tttatctaca tcgccaggct gtatcacctg gcaaacccaa ggaataggca
     9061 gggcctgcgc gatctgatca gctccggcgt gaccatccag atcatgacag agcaggagtc
     9121 cggctactgc tggcacaact tcgtgaatta ttctcctagc aacgagtccc actggcctag
     9181 gtacccacac ctgtgggtgc gcctgtacgt gctggagctg tattgcatca tcctgggcct
     9241 gcccccttgt ctgaatatcc tgcggagaaa gcagagccag ctgacctcct ttacaatcgc
     9301 cctgcagtct tgtcactatc agaggctgcc accccacatc ctgtgggcca caggcctgaa
     9361 gtctggcgga tctagcggag gatcctctgg cagcgagaca ccaggaacaa gcgagtcagc
     9421 aacaccagag agcagtggcg gcagcagcgg cggcagcgac aagaagtaca gcatcggcct
     9481 ggccatcggc accaactctg tgggctgggc cgtgatcacc gacgagtaca aggtgcccag
     9541 caagaaattc aaggtgctgg gcaacaccga ccggcacagc atcaagaaga acctgatcgg
     9601 agccctgctg ttcgacagcg gcgaaacagc cgaggccacc cggctgaaga gaaccgccag
     9661 aagaagatac accagacgga agaaccggat ctgctatctg caagagatct tcagcaacga
     9721 gatggccaag gtggacgaca gcttcttcca cagactggaa gagtccttcc tggtggaaga
     9781 ggataagaag cacgagcggc accccatctt cggcaacatc gtggacgagg tggcctacca
     9841 cgagaagtac cccaccatct accacctgag aaagaaactg gtggacagca ccgacaaggc
     9901 cgacctgcgg ctgatctatc tggccctggc ccacatgatc aagttccggg gccacttcct
     9961 gatcgagggc gacctgaacc ccgacaacag cgacgtggac aagctgttca tccagctggt
    10021 gcagacctac aaccagctgt tcgaggaaaa ccccatcaac gccagcggcg tggacgccaa
    10081 ggccatcctg tctgccagac tgagcaagag cagacggctg gaaaatctga tcgcccagct
    10141 gcccggcgag aagaagaatg gcctgttcgg aaacctgatt gccctgagcc tgggcctgac
    10201 ccccaacttc aagagcaact tcgacctggc cgaggatgcc aaactgcagc tgagcaagga
    10261 cacctacgac gacgacctgg acaacctgct ggcccagatc ggcgaccagt acgccgacct
    10321 gtttctggcc gccaagaacc tgtccgacgc catcctgctg agcgacatcc tgagagtgaa
    10381 caccgagatc accaaggccc ccctgagcgc ctctatgatc aagagatacg acgagcacca
    10441 ccaggacctg accctgctga aagctctcgt gc
//
