LOCUS       sequence-463544-        9728 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  A-to-G base editor.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9728)
  AUTHORS   Matuszek Z, Arbab M, Kesavan M, Hsu A, Roy JCL, Zhao J, Yu T, 
            Weisburd B, Newby GA, Doherty NJ, Wu M, Shibata S, Cristian A, Tao 
            YA, Fearnley LG, Bahlo M, Rehm HL, Xie J, Gao G, Mouro Pinto R, Liu 
            DR
  TITLE     Base editing of trinucleotide repeats that cause Huntington's 
            disease and Friedreich's ataxia reduces somatic repeat expansions in
            patient cells and in mice.
  JOURNAL   Nat Genet. 2025 May 26. doi: 10.1038/s41588-025-02172-8.
  PUBMED    40419681
REFERENCE   2  (bases 1 to 9728)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Genet. 
            2025 May 26. doi: 10.1038/s41588-025-02172-8."
COMMENT     Sequence Label: p2T-CMV-ABE8e_dNRCH-BlastR
FEATURES             Location/Qualifiers
     source          1..9728
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(324..344)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(393..412)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(394..412)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(454..657)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(483..503)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        658..1037
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     misc_feature    join(1046..1183,1184..1195)
                     /label=Tol2 right end
                     /note="right end of the Tol2 transposon from the medaka 
                     fish Oryzias latipes (Kawakami, 2007)"
     primer_bind     1387..1406
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        1485..1589
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1590..1658,1659..2450)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1808..1827)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2621..3209
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3110..3129
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     misc_feature    join(3270..3281,3282..3469)
                     /label=Tol2 left end
                     /note="left end of the Tol2 transposon from the medaka fish
                     Oryzias latipes (Kawakami, 2007)"
     primer_bind     complement(3588..3608)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        3593..3922
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      3773..3908
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     3835..3854
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        3970..4017
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             4036..4434
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     polyA_signal    4592..4713
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4629..4648)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4683..4702
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     protein_bind    4830..4851
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(4866..4871,4872..4889,4890..4896)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4904..4920
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4909..4931
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     4928..4944
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4928..4944
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     polyA_signal    5015..5239
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     5228..5245
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     CDS             complement(5268..5285)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(5294..5314)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(5357..9457)
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLTIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMVKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGIIPHQIHLGELHAILRRQGDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRLRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGGH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKGNSDKLIARKKDWDPKKYGGFNSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKKVKKDLIIKLPKYSLFELENGRKRMLASAGVLQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTINRKQYNTTKEVLD
                     ATLIRQSITGLYETRIDLSQLGGD"
ORIGIN
        1 gagttcctca cgccaaacac cacgcggccg atcctagagt ggatcatggc gccggcgcac
       61 atcacgcaag gctcgaatgt cacgtacagg gtggcgtcaa tcagtctgta gttctgcatg
      121 accaggccgc cctgtctcag ggccataatt tcggcatggg ctgttgggtc gtgcaggccg
      181 atggctctgt tccagccctc gccgatcact ctattgttca gcaccagcac ggctcccaca
      241 ggcacctccc tctcatcccg tgccctcttg gccagggtca gggcatgtct catccagtac
      301 tcgtgggaaa actccacctc agagactttc cgcttcttct ttggtgactc gaactcgctt
      361 ccgtcggctg tccgtttcat ggtggcggct ctccctatag tgagtcgtat tagcggccgc
      421 ggatctctag cggatctgac ggttcactaa accagctctg cttatataga cctcccaccg
      481 tacacgccta ccgcccattt gcgtcaatgg ggcggagttg ttacgacatt ttggaaagtc
      541 ccgttgattt tggtgccaaa acaaactccc attgacgtca atggggtgga gacttggaaa
      601 tccccgtgag tcaaaccgct atccacgccc attgatgtac tgccaaaacc gcatcaccat
      661 ggtaatagcg atgactaata cgtagatgta ctgccaagta ggaaagtccc ataaggtcat
      721 gtactgggca taatgccagg cgggccattt accgtcattg acgtcaatag ggggcgtact
      781 tggcatatga tacacttgat gtactgccaa gtgggcagtt taccgtaaat actccaccca
      841 ttgacgtcaa tggaaagtcc ctattggcgt tactatggga acatacgtca ttattgacgt
      901 caatgggcgg gggtcgttgg gcggtcagcc aggcgggcca tttaccgtaa gttatgtaac
      961 gcggaactcc atatatgggc tatgaactaa tgaccccgta attgattact attaataact
     1021 agtcaataat caatgtcaac gcgttaatac tcaagtacaa ttttaatgga gtactttttt
     1081 acttttactc aagtaagatt ctagccagat acttttactt ttaattgagt aaaattttcc
     1141 ctaagtactt gtactttcac ttgagtaaaa tttttgagta ctttttacac ctctgtcaag
     1201 aaccatatgc cggtacccgc gtatatctgg cccgtacatc gcgaagcagc gcaaaacgcc
     1261 taaccctaag cagattcttc atgcaattgt cggtcaagcc ttgccttgtt gtagcttaaa
     1321 ttttgctcgc gcactactca gcgacctcca acacacaagc agggagcaga tactggctta
     1381 actatgcggc atcagagcag attgtactga gagtcgaccc taggggatcg ggagatcgat
     1441 ctcccgatcc gtcgacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt
     1501 gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa
     1561 tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta
     1621 ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag
     1681 taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca
     1741 gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta
     1801 aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc
     1861 gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc
     1921 ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca
     1981 ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc
     2041 acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca
     2101 taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac
     2161 tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg
     2221 cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg
     2281 ataaatctgg agccggtgag cgtgggtccc gcggtatcat tgcagcactg gggccagatg
     2341 gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac
     2401 gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc
     2461 aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct
     2521 aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc
     2581 actgagtgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc
     2641 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg
     2701 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa
     2761 atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc
     2821 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt
     2881 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa
     2941 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc
     3001 tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc
     3061 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct
     3121 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat
     3181 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc
     3241 tggccttttg ctggcctttt gctcacatgc agaggtgcaa agtacttgag taattttact
     3301 tgattactgt acttaagtat tatttttggg gatttttact ttacttgagt acaattaaaa
     3361 atcaatactt ttacttttac ttaattacat ttttttagaa aaaaaagtac tttttactcc
     3421 ttacaatttt atttacagtc aaaaagtact tattttttgg agatcactta catgttcttt
     3481 cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc
     3541 gctcgccgca gccgaacgac cgagcgcagc gagtcagtga attctgtgga atgtgtgtca
     3601 gttagggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct
     3661 caattagtca gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca
     3721 aagcatgcat ctcaattagt cagcaaccat agtcccgccc ctaactccgc ccatcccgcc
     3781 cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt tttttattta
     3841 tgcagaggcc gaggccgcct ctgcctctga gctattccag aagtagtgag gaggcttttt
     3901 tggaggccta ggcttttgca aaaagctccc gggagcttgt atatccattt tcggatctga
     3961 tcagcacgtg ttgacaatta atcatcggca tagtatatcg gcatagtata atacgacaag
     4021 gtgaggaact aaaccatggc caagcctttg tctcaagaag aatccaccct cattgaaaga
     4081 gcaacggcta caatcaacag catccccatc tctgaagact acagcgtcgc cagcgcagct
     4141 ctctctagcg acggccgcat cttcactggt gtcaatgtat atcattttac tgggggacct
     4201 tgtgcagaac tcgtggtgct gggcactgct gctgctgcgg cagctggcaa cctgacttgt
     4261 atcgtcgcga tcggaaatga gaacaggggc atcttgagcc cctgcggacg gtgccgacag
     4321 gtgcttctcg atctgcatcc tgggatcaaa gccatagtga aggacagtga tggacagccg
     4381 acggcagttg ggattcgtga attgctgccc tctggttatg tgtgggaggg ctaagcactt
     4441 cgtggccgag gagcaggact gacacgtgct acgagatttc gattccaccg ccgccttcta
     4501 tgaaaggttg ggcttcggaa tcgttttccg ggacgccggc tggatgatcc tccagcgcgg
     4561 ggatctcatg ctggagttct tcgcccaccc caacttgttt attgcagctt ataatggtta
     4621 caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag
     4681 ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tgtagcgccc aatacgcaaa
     4741 ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac
     4801 tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt agctcactca ttaggcaccc
     4861 taggctttac actttatgct tccggctcgt atgttgtgtg gaattgtgag cggataacaa
     4921 tttcacacag gaaacagcta tgaccatgat tacgccaagc tctagctaga ggtcgacggt
     4981 atcgagcccc agctggttct ttccgcctca gaagccatag agcccaccgc atccccagca
     5041 tgcctgctat tgtcttccca atcctccccc ttgctgtcct gccccacccc accccccaga
     5101 atagaatgac acctactcag acaatgcgat gcaatttcct cattttatta ggaaaggaca
     5161 gtgggagtgg caccttccag ggtcaaggaa ggcacggggg aggggcaaac aacagatggc
     5221 tggcaactag aaggcacagt cgaggctgat cagcgggttt aaactcaatg gtgatggtga
     5281 tgatgaccgg ttagactttc ctcttcttct tgggctcgaa ttcgctgccg tcggcggttc
     5341 tttttgagcc gccagagtca cctcccagct gagacaggtc gatccgtgtc tcgtacaggc
     5401 cggtgatgct ctgacggatc agggtggcgt ccagcacctc tttggtcgtg ttgtattgct
     5461 tccggttgat ggtggtgtca aagtacttga aggcggcagg ggctcccaga ttggtcaggg
     5521 taaacaggtg gatgatattc tcggcctgct ctctgatggg cttatcccgg tgcttgttgt
     5581 aggcggacag cactttgtcc agattagcgt cggccaggat cactctcttg gagaactcgc
     5641 tgatctgctc gatgatctcg tccaggtagt gcttgtgctg ttccacaaac agctgtttct
     5701 gctcattatc ctcgggggag cccttcagct tctcatagtg gctggccagg tacaggaagt
     5761 tcacatattt ggagggcagg gccagttcgt ttcccttctg cagcacgccg gcagaggcca
     5821 gcattctctt ccggccgttt tccagctcga acagggagta cttaggcagc ttgatgatca
     5881 ggtccttttt cacttttttg tagcccttgg cttccagaaa gtcgatggga ttcttctcga
     5941 agctgcttct ttccatgatg gtgatcccca gcagctcttt cacactcttc agtttcttgg
     6001 acttgccctt ttccactttg gccaccacca gcacagaata ggccacggtg gggctgttga
     6061 agccgccgta cttcttaggg tcccagtcct tctttctggc gatcagctta tcgctgttac
     6121 ccttgggcag gatagactct ttgctgaagc cgcctgtctg cacctcggtc tttttcacga
     6181 tattcacttg gggcatgctc agcactttcc gcacggtggc aaaatcccgg cccttatccc
     6241 acacgatctc cccggtttcg ccgtttgtct cgatcagagg ccgcttccgg atctcgccgt
     6301 tggccagggt aatctcggtc ttgaaaaagt tcatgatgtt gctgtagaag aagtacttgg
     6361 cggtagcctt gccgatttcc tgctcgctct tggcgatcat cttccgcacg tcgtacacct
     6421 tgtagtcgcc gtacacgaac tcgctttcca gcttagggta ctttttgatc agggcggttc
     6481 ccacgacggc gttcaggtag gcgtcgtggg cgtggtggta gttgttgatc tcgcgcactt
     6541 tgtaaaactg gaaatccttc cggaaatcgg acaccagctt ggacttcagg gtgatcactt
     6601 tcacttcccg gatcagcttg tcattctcgt cgtacttagt gttcatccgg gagtccagga
     6661 tctgtgccac gtgctttgtg atctgccggg tttccaccag ctgtctcttg atgaagccgg
     6721 ccttatccag ttcgctcagg ccgcctctct cggccttggt cagattgtcg aactttctct
     6781 gggtaatcag cttggcgttc agcagctgcc gccagtagtt cttcatcttc ttcacgacct
     6841 cttcggaggg cacgttgtcg ctcttgcccc ggttcttgtc gcttctggtc agcaccttgt
     6901 tgtcgatgga gtcgtccttc agaaagctct gaggcacgat agcgtccaca tcgtagtcgg
     6961 acagccggtt gatgtccagt tcctggtcca cgtacatatc ccgcccattc tgcaggtagt
     7021 acaggtacag cttctcgttc tgcagctggg tgttttccac ggggtgttct ttcaggatct
     7081 ggctgcccag ctctttgatg ccctcttcga tccgcttcat tctctcgcgg ctgttcttct
     7141 gtcccttctg ggtggtctgg ttctctctgg ccatttcgat cacgatgttc tcgggcttgt
     7201 ggccgcccat cactttcacg agctcgtcca ccaccttcac tgtctgcagg atgcccttct
     7261 taatggcggg gctgccggcc agattggcaa tgtgctcgtg caggctatcg ccctggccgg
     7321 acacctgggc tttctggatg tcctctttaa aggtcaggct gtcgtcgtgg atcagctgca
     7381 tgaagtttct gttggcgaag ccgtcggact tcaggaaatc caggattgtc ttgccggact
     7441 gcttgtcccg gatgccgttg atcagcttcc ggctcagcct gccccagccg gtgtatctca
     7501 gccgcttcag ctgcttcatc actttgtcgt cgaacaggtg ggcataggtt ttcagccgtt
     7561 cctcgatcat ctctctgtcc tcaaacagtg tcagggtcag cacgatatct tccagaatgt
     7621 cctcgttttc ctcattgtcc aggaagtcct tgtccttgat aattttcagc agatcgtggt
     7681 atgtgcccag ggaggcgttg aaccgatctt ccacgccgga gatttccacg gagtcgaagc
     7741 actcgatttt cttgaagtag tcctctttca gctgcttcac ggtcactttc cggttggtct
     7801 tgaacagcag gtccacgatg gcctttttct gctcgccgct caggaaggcg ggctttctca
     7861 ttccctcggt cacgtatttc actttggtca gctcgttata cacggtgaag tactcgtaca
     7921 gcaggctgtg cttgggcagc accttctcgt tgggcaggtt cttatcgaag ttggtcatcc
     7981 gctcgatgaa gctctgggcg gaagcgccct tgtccaccac ttcctcgaag ttccaggggg
     8041 tgatggtttc ctcgctcttt ctggtcatcc aggcgaatct gctgtttccc ctggccagag
     8101 ggcccacgta gtaggggatg cggaaggtca ggatcttctc gatcttttcc cggttgtcct
     8161 tcaggaatgg gtaaaaatcg ccctgccgcc gcagaatggc gtgcagctct cccaggtgga
     8221 tctggtgggg gataatgccg ttgtcgaagg tccgctgctt ccgcagcagg tcctctctgt
     8281 tcagcttcac gagcagttcc tcggtgccgt ccatcttttc caggatgggc ttgatgaact
     8341 tgtagaactc ttcctggctg gctccgccgt caatgtagcc ggcgtagccg ttcttgctct
     8401 ggtcgaagaa aatctctttg tacttctcag gcagctgctg ccgcacgaga gctttcagca
     8461 gggtcaggtc ctggtggtgc tcgtcgtatc tcttcaccat agaggcgctc aggggggcct
     8521 tggtgatctc ggtgttcact ctcaggatgt cgctcagcag gatggcgtcg gacaggttct
     8581 tggcggccag aaacaggtcg gcgtactggt cgccgatctg ggccagcagg ttgtccaggt
     8641 cgtcgtcgta ggtgtccttg ctcagctgca gtttggcatc ctcggccagg tcgaagttgc
     8701 tcttgaagtt gggggtcagg cccaggctca gggcaatcag gtttccgaac aggccattct
     8761 tcttctcgcc gggcagctgg gcgatcagat tttccagccg tctgctcttg ctcagtctgg
     8821 cagacaggat ggccttggcg tccacgccgc tggcgttgat ggggttttcc tcgaacagct
     8881 ggttgtaggt ctgcaccagc tggatgaaca gcttgtccac gtcgctgttg tcggggttca
     8941 ggtcgccctc gatcaggaag tggccccgga acttgatcat gtgggccagg gccagataga
     9001 tcagccgcag gtcggccttg tcggtgctgt ccaccagttt ctttctcagg tggtagatgg
     9061 tggggtactt ctcgtggtag gccacctcgt ccacgatgtt gccgaagatg gggtgccgct
     9121 cgtgcttctt atcctcttcc accaggaagg actcttccag tctgtggaag aagctgtcgt
     9181 ccaccttggc catctcgttg ctgaagatct cttgcagata gcagatccgg ttcttccgtc
     9241 tggtgtatct tcttctggcg gttctcttca gccgggtggc ctcggctgtt tcgccgctgt
     9301 cgaacagcag ggctccgatc aggttcttct tgatgctgtg ccggtcggtg ttgcccagca
     9361 ccttgaattt cttgctgggc accttgtact cgtcggtgat cacggcccag cccacagagt
     9421 tggtgccgat ggtcaggccg atgctgtact tcttgtctga ccccccgctg ctgcccccgc
     9481 tgctttcagg tgttgcgctc tcgcttgtgc caggtgtctc agagccagag gagcctccgc
     9541 tacttccacc actgttgatg gagctctggg ccttcttctg agcattgaac acctgtctag
     9601 gcatccgata gaaatcgcac agcagggcgg cacattcatc tgccaggatt ccctcggtaa
     9661 tttcgacgcg gtgattcatg ccggggtagt tcagcacgtt catcagggag cctgcggcgc
     9721 ctcttttt
//
