LOCUS       sequence-463545-       10400 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  C-to-T base editor.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10400)
  AUTHORS   Matuszek Z, Arbab M, Kesavan M, Hsu A, Roy JCL, Zhao J, Yu T, 
            Weisburd B, Newby GA, Doherty NJ, Wu M, Shibata S, Cristian A, Tao 
            YA, Fearnley LG, Bahlo M, Rehm HL, Xie J, Gao G, Mouro Pinto R, Liu 
            DR
  TITLE     Base editing of trinucleotide repeats that cause Huntington's 
            disease and Friedreich's ataxia reduces somatic repeat expansions in
            patient cells and in mice.
  JOURNAL   Nat Genet. 2025 May 26. doi: 10.1038/s41588-025-02172-8.
  PUBMED    40419681
REFERENCE   2  (bases 1 to 10400)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Genet. 
            2025 May 26. doi: 10.1038/s41588-025-02172-8."
COMMENT     Sequence Label: p2T-CMV-EA-evoAPOBEC1max-32NLS_NG-BlastR
FEATURES             Location/Qualifiers
     source          1..10400
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             4057..4305
                     /codon_start=1
                     /product="uracil-DNA glycosylase inhibitor from a Bacillus 
                     subtilis bacteriophage (Mol et al., 1995)"
                     /label=UGI
                     /translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTA
                     YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
     CDS             4336..4584
                     /codon_start=1
                     /product="uracil-DNA glycosylase inhibitor from a Bacillus 
                     subtilis bacteriophage (Mol et al., 1995)"
                     /label=UGI
                     /translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTA
                     YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
     CDS             4627..4647
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4656..4673
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(4696..4713)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4702..4926
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(4997..5013)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4997..5013)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5010..5032)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5021..5037
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5045..5051,5052..5069,5070..5075))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5090..5111
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     polyA_signal    5228..5349
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5239..5258)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     5293..5312
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(5507..5905)
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     promoter        complement(5924..5971)
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     promoter        complement(6019..6215)
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        complement(6027..6237)
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      6033..6168
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6087..6106)
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     6261..6281
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     misc_feature    join(6400..6587,6588..6599)
                     /label=Tol2 left end
                     /note="left end of the Tol2 transposon from the medaka fish
                     Oryzias latipes (Kawakami, 2007)"
     rep_origin      complement(6660..7248)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6740..6759)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7419..8210,8211..8279))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8042..8061
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8280..8384)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(8463..8482)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     misc_feature    join(8674..8685,8686..8823)
                     /label=Tol2 right end
                     /note="right end of the Tol2 transposon from the medaka 
                     fish Oryzias latipes (Kawakami, 2007)"
     enhancer        8832..9211
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        9212..9415
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     9366..9386
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     9457..9476
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        9457..9475
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             9525..9545
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             10245..10265
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             join(10326..10400,1..4026)
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SIRPKRNSDKLIARKKDWDPKKYGGFVSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASARFLQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPRAFKYFDTTIDRKVYRSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
ORIGIN
        1 gtgcccagca agaaattcaa ggtgctgggc aacaccgacc ggcacagcat caagaagaac
       61 ctgatcggag ccctgctgtt cgacagcggc gaaacagccg aggccacccg gctgaagaga
      121 accgccagaa gaagatacac cagacggaag aaccggatct gctatctgca agagatcttc
      181 agcaacgaga tggccaaggt ggacgacagc ttcttccaca gactggaaga gtccttcctg
      241 gtggaagagg ataagaagca cgagcggcac cccatcttcg gcaacatcgt ggacgaggtg
      301 gcctaccacg agaagtaccc caccatctac cacctgagaa agaaactggt ggacagcacc
      361 gacaaggccg acctgcggct gatctatctg gccctggccc acatgatcaa gttccggggc
      421 cacttcctga tcgagggcga cctgaacccc gacaacagcg acgtggacaa gctgttcatc
      481 cagctggtgc agacctacaa ccagctgttc gaggaaaacc ccatcaacgc cagcggcgtg
      541 gacgccaagg ccatcctgtc tgccagactg agcaagagca gacggctgga aaatctgatc
      601 gcccagctgc ccggcgagaa gaagaatggc ctgttcggaa acctgattgc cctgagcctg
      661 ggcctgaccc ccaacttcaa gagcaacttc gacctggccg aggatgccaa actgcagctg
      721 agcaaggaca cctacgacga cgacctggac aacctgctgg cccagatcgg cgaccagtac
      781 gccgacctgt ttctggccgc caagaacctg tccgacgcca tcctgctgag cgacatcctg
      841 agagtgaaca ccgagatcac caaggccccc ctgagcgcct ctatgatcaa gagatacgac
      901 gagcaccacc aggacctgac cctgctgaaa gctctcgtgc ggcagcagct gcctgagaag
      961 tacaaagaga ttttcttcga ccagagcaag aacggctacg ccggctacat tgacggcgga
     1021 gccagccagg aagagttcta caagttcatc aagcccatcc tggaaaagat ggacggcacc
     1081 gaggaactgc tcgtgaagct gaacagagag gacctgctgc ggaagcagcg gaccttcgac
     1141 aacggcagca tcccccacca gatccacctg ggagagctgc acgccattct gcggcggcag
     1201 gaagattttt acccattcct gaaggacaac cgggaaaaga tcgagaagat cctgaccttc
     1261 cgcatcccct actacgtggg ccctctggcc aggggaaaca gcagattcgc ctggatgacc
     1321 agaaagagcg aggaaaccat caccccctgg aacttcgagg aagtggtgga caagggcgct
     1381 tccgcccaga gcttcatcga gcggatgacc aacttcgata agaacctgcc caacgagaag
     1441 gtgctgccca agcacagcct gctgtacgag tacttcaccg tgtataacga gctgaccaaa
     1501 gtgaaatacg tgaccgaggg aatgagaaag cccgccttcc tgagcggcga gcagaaaaag
     1561 gccatcgtgg acctgctgtt caagaccaac cggaaagtga ccgtgaagca gctgaaagag
     1621 gactacttca agaaaatcga gtgcttcgac tccgtggaaa tctccggcgt ggaagatcgg
     1681 ttcaacgcct ccctgggcac ataccacgat ctgctgaaaa ttatcaagga caaggacttc
     1741 ctggacaatg aggaaaacga ggacattctg gaagatatcg tgctgaccct gacactgttt
     1801 gaggacagag agatgatcga ggaacggctg aaaacctatg cccacctgtt cgacgacaaa
     1861 gtgatgaagc agctgaagcg gcggagatac accggctggg gcaggctgag ccggaagctg
     1921 atcaacggca tccgggacaa gcagtccggc aagacaatcc tggatttcct gaagtccgac
     1981 ggcttcgcca acagaaactt catgcagctg atccacgacg acagcctgac ctttaaagag
     2041 gacatccaga aagcccaggt gtccggccag ggcgatagcc tgcacgagca cattgccaat
     2101 ctggccggca gccccgccat taagaagggc atcctgcaga cagtgaaggt ggtggacgag
     2161 ctcgtgaaag tgatgggccg gcacaagccc gagaacatcg tgatcgaaat ggccagagag
     2221 aaccagacca cccagaaggg acagaagaac agccgcgaga gaatgaagcg gatcgaagag
     2281 ggcatcaaag agctgggcag ccagatcctg aaagaacacc ccgtggaaaa cacccagctg
     2341 cagaacgaga agctgtacct gtactacctg cagaatgggc gggatatgta cgtggaccag
     2401 gaactggaca tcaaccggct gtccgactac gatgtggacc atatcgtgcc tcagagcttt
     2461 ctgaaggacg actccatcga caacaaggtg ctgaccagaa gcgacaagaa ccggggcaag
     2521 agcgacaacg tgccctccga agaggtcgtg aagaagatga agaactactg gcggcagctg
     2581 ctgaacgcca agctgattac ccagagaaag ttcgacaatc tgaccaaggc cgagagaggc
     2641 ggcctgagcg aactggataa ggccggcttc atcaagagac agctggtgga aacccggcag
     2701 atcacaaagc acgtggcaca gatcctggac tcccggatga acactaagta cgacgagaat
     2761 gacaagctga tccgggaagt gaaagtgatc accctgaagt ccaagctggt gtccgatttc
     2821 cggaaggatt tccagtttta caaagtgcgc gagatcaaca actaccacca cgcccacgac
     2881 gcctacctga acgccgtcgt gggaaccgcc ctgatcaaaa agtaccctaa gctggaaagc
     2941 gagttcgtgt acggcgacta caaggtgtac gacgtgcgga agatgatcgc caagagcgag
     3001 caggaaatcg gcaaggctac cgccaagtac ttcttctaca gcaacatcat gaactttttc
     3061 aagaccgaga ttaccctggc caacggcgag atccggaagc ggcctctgat cgagacaaac
     3121 ggcgaaaccg gggagatcgt gtgggataag ggccgggatt ttgccaccgt gcggaaagtg
     3181 ctgagcatgc cccaagtgaa tatcgtgaaa aagaccgagg tgcagacagg cggcttcagc
     3241 aaagagtcta tccggcccaa gaggaacagc gataagctga tcgccagaaa gaaggactgg
     3301 gaccctaaga agtacggcgg cttcgtcagc cccaccgtgg cctattctgt gctggtggtg
     3361 gccaaagtgg aaaagggcaa gtccaagaaa ctgaagagtg tgaaagagct gctggggatc
     3421 accatcatgg aaagaagcag cttcgagaag aatcccatcg actttctgga agccaagggc
     3481 tacaaagaag tgaaaaagga cctgatcatc aagctgccta agtactccct gttcgagctg
     3541 gaaaacggcc ggaagagaat gctggcctct gcccgcttcc tgcagaaggg aaacgaactg
     3601 gccctgccct ccaaatatgt gaacttcctg tacctggcca gccactatga gaagctgaag
     3661 ggctcccccg aggataatga gcagaaacag ctgtttgtgg aacagcacaa gcactacctg
     3721 gacgagatca tcgagcagat cagcgagttc tccaagagag tgatcctggc cgacgctaat
     3781 ctggacaaag tgctgtccgc ctacaacaag caccgggata agcccatcag agagcaggcc
     3841 gagaatatca tccacctgtt taccctgacc aatctgggag cccctcgcgc cttcaagtac
     3901 tttgacacca ccatcgaccg gaaggtgtac cgcagcacca aagaggtgct ggacgccacc
     3961 ctgatccacc agagcatcac cggcctgtac gagacacgga tcgacctgtc tcagctggga
     4021 ggtgacagcg gcgggagcgg cgggagcggg gggagcacta atctgagcga catcattgag
     4081 aaggagactg ggaaacagct ggtcattcag gagtccatcc tgatgctgcc tgaggaggtg
     4141 gaggaagtga tcggcaacaa gccagagtct gacatcctgg tgcacaccgc ctacgacgag
     4201 tccacagatg agaatgtgat gctgctgacc tctgacgccc ccgagtataa gccttgggcc
     4261 ctggtcatcc aggattctaa cggcgagaat aagatcaaga tgctgagcgg aggatccgga
     4321 ggatctggag gcagcaccaa cctgtctgac atcatcgaga aggagacagg caagcagctg
     4381 gtcatccagg agagcatcct gatgctgccc gaagaagtcg aagaagtgat cggaaacaag
     4441 cctgagagcg atatcctggt ccataccgcc tacgacgaga gtaccgacga aaatgtgatg
     4501 ctgctgacat ccgacgcccc agagtataag ccctgggctc tggtcatcca ggattccaac
     4561 ggagagaaca aaatcaaaat gctgtctggc ggctcaaaaa gaaccgccga cggcagcgaa
     4621 ttcgagccca agaagaagag gaaagtctaa ccggtcatca tcaccatcac cattgagttt
     4681 aaacccgctg atcagcctcg actgtgcctt ctagttgcca gccatctgtt gtttgcccct
     4741 cccccgtgcc ttccttgacc ctggaaggtg ccactcccac tgtcctttcc taataaaatg
     4801 aggaaattgc atcgcattgt ctgagtaggt gtcattctat tctggggggt ggggtggggc
     4861 aggacagcaa gggggaggat tgggaagaca atagcaggca tgctggggat gcggtgggct
     4921 ctatggcttc tgaggcggaa agaaccagct ggggctcgat accgtcgacc tctagctaga
     4981 gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc
     5041 cacacaacat acgagccgga agcataaagt gtaaagccta gggtgcctaa tgagtgagct
     5101 aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc
     5161 agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctaca
     5221 gacatgataa gatacattga tgagtttgga caaaccacaa ctagaatgca gtgaaaaaaa
     5281 tgctttattt gtgaaatttg tgatgctatt gctttatttg taaccattat aagctgcaat
     5341 aaacaagttg gggtgggcga agaactccag catgagatcc ccgcgctgga ggatcatcca
     5401 gccggcgtcc cggaaaacga ttccgaagcc caacctttca tagaaggcgg cggtggaatc
     5461 gaaatctcgt agcacgtgtc agtcctgctc ctcggccacg aagtgcttag ccctcccaca
     5521 cataaccaga gggcagcaat tcacgaatcc caactgccgt cggctgtcca tcactgtcct
     5581 tcactatggc tttgatccca ggatgcagat cgagaagcac ctgtcggcac cgtccgcagg
     5641 ggctcaagat gcccctgttc tcatttccga tcgcgacgat acaagtcagg ttgccagctg
     5701 ccgcagcagc agcagtgccc agcaccacga gttctgcaca aggtccccca gtaaaatgat
     5761 atacattgac accagtgaag atgcggccgt cgctagagag agctgcgctg gcgacgctgt
     5821 agtcttcaga gatggggatg ctgttgattg tagccgttgc tctttcaatg agggtggatt
     5881 cttcttgaga caaaggcttg gccatggttt agttcctcac cttgtcgtat tatactatgc
     5941 cgatatacta tgccgatgat taattgtcaa cacgtgctga tcagatccga aaatggatat
     6001 acaagctccc gggagctttt tgcaaaagcc taggcctcca aaaaagcctc ctcactactt
     6061 ctggaatagc tcagaggcag aggcggcctc ggcctctgca taaataaaaa aaattagtca
     6121 gccatggggc ggagaatggg cggaactggg cggagttagg ggcgggatgg gcggagttag
     6181 gggcgggact atggttgctg actaattgag atgcatgctt tgcatacttc tgcctgctgg
     6241 ggagcctggg gactttccac accctaactg acacacattc cacagaattc actgactcgc
     6301 tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt
     6361 tatccacaga atcaggggat aacgcaggaa agaacatgta agtgatctcc aaaaaataag
     6421 tactttttga ctgtaaataa aattgtaagg agtaaaaagt actttttttt ctaaaaaaat
     6481 gtaattaagt aaaagtaaaa gtattgattt ttaattgtac tcaagtaaag taaaaatccc
     6541 caaaaataat acttaagtac agtaatcaag taaaattact caagtacttt acacctctgc
     6601 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt
     6661 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg
     6721 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc
     6781 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc
     6841 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc
     6901 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac
     6961 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt
     7021 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct
     7081 aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc
     7141 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt
     7201 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg
     7261 atcttttcta cggggtctga cactcagtgg aacgaaaact cacgttaagg gattttggtc
     7321 atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa
     7381 tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag
     7441 gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg
     7501 tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcgg
     7561 gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag
     7621 cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa
     7681 gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc
     7741 atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca
     7801 aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg
     7861 atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat
     7921 aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc
     7981 aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg
     8041 gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg
     8101 gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt
     8161 gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca
     8221 ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata
     8281 ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac
     8341 atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa
     8401 gtgccacctg acgtcgacgg atcgggagat cgatctcccg atcccctagg gtcgactctc
     8461 agtacaatct gctctgatgc cgcatagtta agccagtatc tgctccctgc ttgtgtgttg
     8521 gaggtcgctg agtagtgcgc gagcaaaatt taagctacaa caaggcaagg cttgaccgac
     8581 aattgcatga agaatctgct tagggttagg cgttttgcgc tgcttcgcga tgtacgggcc
     8641 agatatacgc gggtaccggc atatggttct tgacagaggt gtaaaaagta ctcaaaaatt
     8701 ttactcaagt gaaagtacaa gtacttaggg aaaattttac tcaattaaaa gtaaaagtat
     8761 ctggctagaa tcttacttga gtaaaagtaa aaaagtactc cattaaaatt gtacttgagt
     8821 attaacgcgt tgacattgat tattgactag ttattaatag taatcaatta cggggtcatt
     8881 agtttatagc ccatatatgg agttccgcgt tacataactt acggtaaatg gcccgcctgg
     8941 ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc ccatagtaac
     9001 gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt
     9061 ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca atgacggtaa
     9121 atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta cttggcagta
     9181 catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt acatcaatgg
     9241 gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg acgtcaatgg
     9301 gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca actccgcccc
     9361 attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca gagctggttt
     9421 agtgaaccgt cagatccgct agagatccgc ggccgctaat acgactcact atagggagag
     9481 ccgccaccat gaaacggaca gccgacggaa gcgagttcga gtcaccaaag aagaagcgga
     9541 aagtcagttc aaagactggg cctgtcgccg tcgatccaac cctgcgccgc cggattgaac
     9601 ctcacgagtt tgaagtgttc tttgaccccc gggagctgag aaaggagaca tgcctgctgt
     9661 acgagatcaa ctggggaggc agggaggcca tctggaggca cacctctcag aacacaaata
     9721 agcacgtgga ggtgaacttc atcgagaagt ttaccacaga gcggtacttc tgccccaata
     9781 ccagatgtag catcacatgg tttctgagct ggtccccttg cggagagtgt agcagggcca
     9841 tcaccgagtt cctgtccaga tatccaaatg tgacactgtt tatctacatc gccaggctgt
     9901 atcacctggc aaacccaagg aataggcagg gcctgcgcga tctgatcagc tccggcgtga
     9961 ccatccagat catgacagag caggagtccg gctactgctg gcacaacttc gtgaattatt
    10021 ctcctagcaa cgagtcccac tggcctaggt acccacacct gtgggtgcgc ctgtacgtgc
    10081 tggagctgta ttgcatcatc ctgggcctgc ccccttgtct gaatatcctg cggagaaagc
    10141 agagccagct gacctccttt acaatcgccc tgcagtcttg tcactatcag aggctgccac
    10201 cccacatcct gtgggccaca ggcctgaagt ctggcggatc tgccccaaag aagaagcgga
    10261 aggtcggtat ccacggagtc ccagcagcca gcggaggatc cagtggcggc agcagcggcg
    10321 gcagcgacaa gaagtacagc atcggcctgg ccatcggcac caactctgtg ggctgggccg
    10381 tgatcaccga cgagtacaag
//
