LOCUS       sequence-463594-        1240 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression of human CAPN15 (N)ZF1-3 (1-173).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1240)
  AUTHORS   Michel MA, Scutts S, Komander D
  TITLE     Secondary interactions in ubiquitin-binding domains achieve linkage 
            or substrate specificity.
  JOURNAL   Cell Rep. 2024 Aug 27;43(8):114545. doi: 
            10.1016/j.celrep.2024.114545. Epub 2024 Jul 23.
  PUBMED    39052481
REFERENCE   2  (bases 1 to 1240)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Rep.";
            date: "2024-08-27"; volume: "43(8):114545. doi"; pages: " 
            10.1016/j.celrep.2024.114545. Epub 2024 Jul 23"
COMMENT     Sequence Label: pOPINK-CAPN15 (N)ZF1-3 (1-173)
FEATURES             Location/Qualifiers
     source          1..1240
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(30..47)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(590..613)
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     primer_bind     complement(652..674)
                     /label=pGEX 5'
                     /note="pGEX vectors, Glutathione-S-transferase, forward 
                     primer"
ORIGIN
        1 actcttnngg cttgtnacgc cggatctcag tggtggtggt ggtggtgttt aaatggtcta
       61 gaaagcttta cgagagcctg cgtgggcccc cgcagacgga gcaggagctg gccacgggcg
      121 tgttgtgcag cgtgcaacgc ggacacgccc agccccctct gggctccgcc gctccctcct
      181 cctcctcctg ctcctccttc tcctcctcgt ccttgtcctc gcactgcccc ctggcgggct
      241 ccgtggccac cagccccgca gtcctcactg gacctgcttc ctcctggcag ctgcccttgg
      301 gctcccccag gatggcgggt ggcttgggga ggaccccgtt gaggactggc aggaaggcag
      361 ccccaggcgc aggctccggg gtgaagccgc acacctcgca ggcctccttg cccaggaagt
      421 tgcggaaggt gcagcgggcg cagggccatt tctgctcctc cacgctgagc cgcaggatgt
      481 ggttgaggtc gggcttgtgc cggggagcct cgcagatgga gcactggcgc tggccggccg
      541 ggttcaggaa ggtgcagcgc acacaggacc actctccgac cgtggccatc gggccctgaa
      601 acagaacttc cagaccgctg ctcagatccg attttggagg atggtcgcca ccaccaaacg
      661 tggcttgcca gccctgcaaa ggccatgcta tatacttgct ggatttcaag tacttatcaa
      721 tttgtgggat agcttcaata cgttttttaa aacaaactaa ttttgggaac gcatccaggc
      781 acattgggtc catgtataaa acaacatcaa gagcgtcata caacatgaag tcaggatggg
      841 ttacatgatc accatttaaa tatgttttat gacataaacg atcttcgaac attttcagca
      901 tttcaggtag cttgctaaga aaatcaactt ttgagagttt caaagtcttt actatatgca
      961 attctcgaaa caccgtatct aatatccaaa accgctcctt caagcattga aatctctgca
     1021 cgctcttttg gacaaccacc caacatggtt gtgcttggtc agctatataa ccgaatgaag
     1081 ggccataaga ctggggttaa ttttaacatc nccatccaat ataantaagg gaaganntgg
     1141 ggaaactccc aaaccccaat tncaaacttt tttggtttcc nccattttat ccaccnttca
     1201 tccgcgctcc aancaaangg ncccttccaa ntttttcctt
//
