LOCUS       sequence-463606-        1042 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression of human ZRANB3 NZF (619-650).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1042)
  AUTHORS   Michel MA, Scutts S, Komander D
  TITLE     Secondary interactions in ubiquitin-binding domains achieve linkage 
            or substrate specificity.
  JOURNAL   Cell Rep. 2024 Aug 27;43(8):114545. doi: 
            10.1016/j.celrep.2024.114545. Epub 2024 Jul 23.
  PUBMED    39052481
REFERENCE   2  (bases 1 to 1042)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Rep.";
            date: "2024-08-27"; volume: "43(8):114545. doi"; pages: " 
            10.1016/j.celrep.2024.114545. Epub 2024 Jul 23"
COMMENT     Sequence Label: pOPINK-ZRANB3 NZF (619-650)
FEATURES             Location/Qualifiers
     source          1..1042
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(32..49)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(169..192)
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             complement(211..864)
                     /codon_start=1
                     /product="glutathione S-transferase from Schistosoma 
                     japonicum"
                     /label=GST
                     /translation="MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKK
                     FELGLEFXNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRY
                     GVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVL
                     YMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPK"
     primer_bind     complement(231..253)
                     /label=pGEX 5'
                     /note="pGEX vectors, Glutathione-S-transferase, forward 
                     primer"
     gap             966..980
                     /estimated_length=15
     gap             1019..1037
                     /estimated_length=19
ORIGIN
        1 cnttcnngct ttgttagcag ccggatctcn agtggtggtg gtggtggtgt ttaaatggtc
       61 tagaaagctt taactgcctt gaggagtctc acacatttca caataaggta actctgaatt
      121 attgatatag gtgcagagac tacattgcca gccctctaca ggaaaggccg ggccctgaaa
      181 cagaacttcc agaccgctgc tcagatccga ttttggagga tggtcgccac caccaaacgt
      241 ggcttgccag ccctgcaaag gccatgctat atacttgctg gatttcaagt acttatcaat
      301 ttgtgggata gcttcaatac gttttttaaa acaaactaat tttgggaacg catccaggca
      361 cattgggtcc atgtataaaa caacatcaag agcgtcatac aacatgaagt caggatgggt
      421 tacatgatca ccatttaaat atgttttatg acataaacga tcttcgaaca ttttcagcat
      481 ttcaggtagc ttgctaagaa aatcaacttt gagagtttca aagtctttac tatatgcaat
      541 tctcgaaaca ccgtatctaa tatccaaaac cgctccttca agcattgaaa tctctgcacg
      601 ctcttttgga caaccaccca acatgttgtg cttgtcagct atataacgta tgatggccat
      661 agactgtgtt aatttaacat caccatcaat ataataagga agattgngaa actccaaacc
      721 caattcaaac tttttgtttc gccatttatc accttcatcg cgctcataca aatgctcttc
      781 atatttttct tcaagatatt ccaaaagaag tcgagtgggt tgcacaagnc ccttaatttt
      841 ccaataacct agtatagggg acatgtgatg gtgatgngat gtgccatgnn tatctnnnct
      901 taaagtnaaa caaanntnan ttnnnannnn nggnanntnn tnncccnnnn nnnnntnccc
      961 nannannnnn nnnnnnnnnn anttnnnnnn ncgnnnatnc nnnntnnnct nnnngnnann
     1021 nnnnnnnnnn nnnnnnnccn ng
//
