LOCUS       sequence-463609-       12047 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Protein expression in insect cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12047)
  AUTHORS   Lopez Martinez D, Todorovski I, Noe Gonzalez M, Rusimbi C, Blears D,
            Khallou N, Han Z, Dirac-Svejstrup AB, Svejstrup JQ
  TITLE     PAF1C-mediated activation of CDK12/13 kinase activity is critical 
            for CTD phosphorylation and transcript elongation.
  JOURNAL   Mol Cell. 2025 May 15;85(10):1952-1967.e8. doi: 
            10.1016/j.molcel.2025.04.012. Epub 2025 May 1.
  PUBMED    40315851
REFERENCE   2  (bases 1 to 12047)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2025.04.012"; journalName: "Mol Cell"; date: 
            "2025-05-15- 15"; volume: "85"; issue: "10"; pages: "1952-1967.e8"
COMMENT     Sequence Label: pBIG1e-CDK12deltaCTD/Cyclin K
FEATURES             Location/Qualifiers
     source          1..12047
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(1103..1194)
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     polyA_signal    1350..1484
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(4863..4886)
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             complement(4893..4916)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     promoter        complement(4962..5053)
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     polyA_signal    5148..5196
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     primer_bind     5221..5240
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     protein_bind    5286..5319
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        join(5715..5720,5721..5737,5738..5743)
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     CDS             5932..6465
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     mobile_element  6532..6756
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     primer_bind     complement(6890..6907)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7061..7649)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7141..7160)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7820..8611,8612..8680))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8443..8462
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8681..8785)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(8811..9265)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8948..8969)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     9159..9178
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     CDS             complement(9306..10097)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     mobile_element  10621..10786
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     polyA_signal    11101..11235
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
ORIGIN
        1 taggtggtgg tggttctgct gctttgttct cttctttggg agaaacaacc acggctcgct
       61 taacctgtcg gggagaactg ggctgcggag agggtttctt gggctgttgg gctggctgct
      121 gctgctgggc tggttgctgg gggtccttct gctggggctg ggatggttcg gagctttgag
      181 acggctgtga ctgctgtact tgcggcactt gtggtgtagg ctgaagagat gggggctgtt
      241 gcagctgatg gggggtgtga tgaggcatct gttgttttcc ttgtgagtaa agatccagga
      301 tttggtggca gatgtcttcc aaaacgtcga ccgggacatc ttgaacaaac tgctcccacc
      361 atctcctata catgggtttg gaggtccatt cttgtatttc aaatttgcac aaacgtcctg
      421 cgagatacat cactgctact gctatgatct ctggttccca ctgcagtgac aaggtggtgc
      481 agagactgtc atttacaaat gtccatgcca tttgaaccaa cttttgaatt ttgtttttat
      541 cacctttgag ttgctttgca tattttagta ggaactggta tggatgttct acctgtaaat
      601 caaacttgat ggtctgcagt aagattctct ccagaaccat tacttcctcc tttgggtcat
      661 ctccaaactg gccaaattgt acatcattta ataaactacg agctgttttg atgatatctt
      721 tacatttttt tggtgtttct tctactttcc cagccagaaa gaggcaacag gctcctgtca
      781 catatcttgg gaattgcttg aaggaatgaa acatatagaa gcgatgaaaa taaattattc
      841 cagttgccag ggtatcatag tgtagcccca aacgtgtgcc cacatcaaag atgaaccgag
      901 cgccctctcg gcggtaccgg gcctcggtgg ctggatcaag tccttcaagt tgtgagggtg
      961 tatgagccaa gtctttctta tcccagtacc aacatggctt tgtgtggtcc aggtttgctg
     1021 aagttactga agggcttgaa ttttctttat tctccttcat ggatccgcgc ccgatggtgg
     1081 gacggtatga ataatccgga atatttatag gtttttttat tacaaaactg ttacgaaaac
     1141 agtaaaatac ttatttattt gcgagatggt tatcatttta attatctcca tgatctatta
     1201 atattccgga gtaggtcgat ttaaacgtgc aatagtatcc agtttattta aatggttatg
     1261 atagttattg ctcagcggtg gcagcagcca actcagcttc ctttcgggct ttgttagcag
     1321 ccggatcttc taggctcaag cagtgatcag atccagacat gataagatac attgatgagt
     1381 ttggacaaac cacaactaga atgcagtgaa aaaaatgctt tatttgtgaa atttgtgatg
     1441 ctattgcttt atttgtaacc attataagct gcaataaaca agttaacaac aacaattgca
     1501 ttcattttat gtttcaggtt cagggggagg tgtgggaggt tttttaaagc aagtaaaacc
     1561 tctacaaatg tggtatggct gattatgatc ctctagtact tctcgacaag cttttagctg
     1621 ttcttcacag gctcagtact tgtccctgag gtagtttctt ttcgagaagt tttggatgga
     1681 ggtggctctt cgactacaac accactttgt cgctgacgtc gccgtttctt actccacaac
     1741 tcatggcaat cctgccagtg ggggaggtct ggaggagcca ttttgctgag ttcgacatct
     1801 ttaaggaagt cgctctgtag ggtctgttca gctgtgcacc gcttactagg atctagtgtc
     1861 agcatgtggt ccaataaatc aagtgctgca gaaggaatga aagagaattc ttctcgtaga
     1921 cgccttcgat attgcttctt cggtttcatg gtgttgaagt agggcagttt gataacatca
     1981 ggccacacag ctggacaagg gctaccacaa agtcggctga tcagttctag ctgagccagt
     2041 tccagattgg cttgaaaaat aggcttcttt gtgaatagtt ccccaagaat acatccacag
     2101 ctccaaacat ctatggctgg tgtgtaacgt tcctctccta gcagtagttc tggaggtcgg
     2161 taccacaaag taatgacttt gtttgtgtaa gggcgactct cttcagagtt atagagccga
     2221 gcaagtccaa aatctgctag tttgatttgc ccactgttat tcagcaaaat gttagaacac
     2281 ttaatatccc gatgcaggaa attctttttg tgacagtatt ccaatccttc cattagctgt
     2341 ttcatgaacg acttgatatg gtcctcagaa aagtgcacca aaccagattc tagcagtccc
     2401 attaagtcat ggtccatata ctcaaataca aggtaaaagg cacctttgtc cttcttgaaa
     2461 tccagtgcat cttgtttatc tgtgacaatt tccttcatgt taacaacact tcggtggatt
     2521 aactgacgaa ggattttgat ttcacgaatg gctgtgattg ggaagccctc tttctcattg
     2581 tctagtctca ccttcttcag agccactagt tctcctgtgt ctttgtcctt ggctttatat
     2641 acttggccat aggttccttc tccaataatc ccaataatgt caaacttgtc cacacagcgt
     2701 ttcccccagt cgctttctgt ttgtcttctt tctccataac gaggacaaca aatttttggt
     2761 ctctttttat atggttgctg aggtggtgtg attgcctttg gttctggaga gtctggggga
     2821 gacagatctc caccagggag ctctggaggg agaggaaggt ctgtgagtaa gtgacgtgtc
     2881 ctctgttcct tctctttctt cacaggtttt gaaggaagag tttcttttgg actatccatg
     2941 tcatcatctc caggtaataa gggtgggagg ggcaaaggag gcaacgttga agttttcagg
     3001 tgtggaatag cagctgttac agatacttga gtcttcacag aaacctgtac agggggctga
     3061 gaatttgcct gagaggacac agcagatgtc tttgagtgag tagaaggggg caaagttgaa
     3121 gtactggaag caggaacctg actaaatgct ggctgagaag gaggcagagg tggttgctgt
     3181 ggaagagctg gtattggagg caaaggtggc aaagggggtg tctgaggtgg aggggtagta
     3241 gttggtagag ggggtggggg agaagcaatt gtgggaagag gtggaggggt ctccttttct
     3301 gatgtttctg tctcctttgg agtaacaatc tcctctttca gtgctatggg tttagagtct
     3361 cttgttccct gtgctttcaa atctttaaca agatgcttct cggagttctc atccaacttt
     3421 actttccctg tatctgaaga attttttacc tctgtgttta gatgtgttac attcaccagt
     3481 tcagtatctg gggcagattt ttccaatttt acacttctgg gtaacttttt agactccaaa
     3541 cctgaatcct tagcctctac tgaactgttc tcttttctag gcaaaaatac aggtgaaccc
     3601 ttggactcct ttccatccat ctttgctgca gcagcagcag ctgctctttc cttctttttc
     3661 ctactgagtt cagctcccag actggaatta agtggaagcc tgacaggtga gatactggaa
     3721 tgacgactgc gtgaactgga tctcttcttt ttactatgag aagatgaatg tcttgaatat
     3781 gcaggacttc tacttctgga cttcatggat ttcctactgg ggagtggact ccgactcaga
     3841 gaccgcttgc tcaggaaagg gctgctggac cgccttcgac catagggact gggcgatcgc
     3901 ccgctgtaag agccacttct ttcgtagctg gacgaccgtc tcctgctata gggactgaca
     3961 gatctctgtc gcctactgta ggggctcggt gaccgggtgc tggactggta ggccgaaggc
     4021 tccttgtaag ggggactgac cgactgcctt ctcgaggtac ttccaggact tttcttgtag
     4081 gagtcataat tgctcgaggt atgagatcgt gagggactaa ggtcataatc ttggccataa
     4141 gaagctcccg aggggctatc atcttgtttg gagctgtcag accacttcct gtgggggctc
     4201 ctggatctcc gttttgggct gtccactgtt ttgtaacttt tgggtgtttc cctttttcga
     4261 tgacttttac tccggtcttt gtgcccagac ttcagctccc gttctttcct ggtcttctcc
     4321 ttgtggagct tggatgacct ggattccttg ctgctgcttt tggctacctg cgccttccca
     4381 tagtcatcag tctcctcatt cgaacgcttt gaacttcccg atatccggtc cttcatcgat
     4441 cccgacttgc tggagacttc ttggcttttt tctttttcgg tctgtttagc ttttagtaag
     4501 tcccgggaac gcctgtgctg gtggtgacga tgtttgtgca ggcggtcgct ccgatctgat
     4561 ccacgacgtt cgtcgttctc ccttcggtct agtttgaagg ccatgtcatc ggagaaggtg
     4621 tcggaatcag agctgatatc atcatactcc accaaaggtt tgataactgt gcccagggat
     4681 gctgcttcgg gggtcaccaa ccccatgtct ttggagtgtt tggacttatg ccgcttgtgc
     4741 ttcgatacca agcggtgacg ctctctgctg ttagagctgc cgcctcccga tgacggctgc
     4801 aaagttccag aagctcctcc actcccgtcc ttcttgcccc catgtctctc tgaattgggc
     4861 atgggtccct gaaagaggac ttcaagatcg agcttgtcat cgtcgtcctt gtagtccatg
     4921 gatccgcgcc cgatggtggg acggtatgaa taatccggaa tatttatagg tttttttatt
     4981 acaaaactgt tacgaaaaca gtaaaatact tatttatttg cgagatggtt atcattttaa
     5041 ttatctccat gatctattaa tattccggag taggtcgatt taaatcttta gaccatagag
     5101 cgttcacttg acagagtgcc agccctggga ccgaaccccg cgtttatgaa caaacgaccc
     5161 aacacccgtg cgttttattc tgtcttttta ttgccgtcat agcgcgggtt ccttccggta
     5221 ttgtctcctt ccgtgtttca gttagcctcg ataaaaacta agctatgtga accgtttaaa
     5281 cccagataac ttcgtataat gtatgctata cgaagttatg gtaccgcggc cgcgtagagg
     5341 atctgttgat cagcagttca acctgttgat agtacaaaca aagctgtagc caaccactag
     5401 aactatagct agagtcctgg gcgaacaaac gatgctcgcc ttccagaaaa ccgaggatgc
     5461 gaaccacttc atccggggtc agcaccaccg gcaagcgccg cgacggccga ggtctaccga
     5521 tctcctgaag caagggcaga tccgtgcaca gcaccttgcc gtagaagaac agcaaggccg
     5581 ccaatgcctg acgatgcgtg gagaccgaaa ccttgcgctc gttcgccagc caggacagaa
     5641 atgcctcgac ttcgctgctg cccaaggttg ccgggtgacg cacaccgtgg aaacggatga
     5701 aggcacaaac ccagttgaca taagcctgtt cggttcgtaa actgtaatgc aagtagcgta
     5761 tgcgctcacg caactggtcc agaaccttga ccgaacgcag cggtggtaac ggcgcagtgg
     5821 cggttttcat ggcttgttat gactgttttt ttgtacagtc tatgcctcgg gcatccaagc
     5881 agcaagcgcg ttacgccgtg ggtcgatgtt tgatgttatg gagcagcaac gatgttacgc
     5941 agcagcaacg atgttacgca gcagggcagt cgccctaaaa caaagttagg tggctcaagt
     6001 atgggcatca ttcgcacatg taggctcggc cctgaccaag tcaaatccat gcgggctgct
     6061 cttgatcttt tcggtcgtga gttcggagac gtagccacct actcccaaca tcagccggac
     6121 tccgattacc tcgggaactt gctccgtagt aagacattca tcgcgcttgc tgccttcgac
     6181 caagaagcgg ttgttggcgc tctcgcggct tacgttctgc ccaagtttga gcagccgcgt
     6241 agtgagatct atatctatga tctcgcagtc tccggcgagc accggaggca gggcattgcc
     6301 accgcgctca tcaatctcct caagcatgag gccaacgcgc ttggtgctta tgtgatctac
     6361 gtgcaagcag attacggtga cgatcccgca gtggctctct atacaaagtt gggcatacgg
     6421 gaagaagtga tgcactttga tatcgaccca agtaccgcca cctaacaatt cgttcaagcc
     6481 gagatcggct tcccggccgc ggagttgttc ggtaaattgt cacaacgccg cgaatatagt
     6541 ctttaccatg cccttggcca cgcccctctt taatacgacg ggcaatttgc acttcagaaa
     6601 atgaagagtt tgctttagcc ataacaaaag tccagtatgc tttttcacag cataactgga
     6661 ctgatttcag tttacaacta ttctgtctag tttaagactt tattgtcata gtttagatct
     6721 attttgttca gtttaagact ttattgtccg cccacacccg cttacgcagg gcatccattt
     6781 attactcaac cgtaaccgat tttgccaggt tacgcggctg gtctatgcgg tgtgaaatac
     6841 cgcacagatg cgtaaggaga aaataccgca tcaggcgctc ttccgcttcc tcgctcactg
     6901 actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa
     6961 tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc
     7021 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc
     7081 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat
     7141 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc
     7201 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct
     7261 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg
     7321 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc
     7381 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga
     7441 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa
     7501 gaatagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta
     7561 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc
     7621 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg
     7681 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga
     7741 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg
     7801 agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct
     7861 gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg
     7921 agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc
     7981 cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa
     8041 ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc
     8101 cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt
     8161 cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc
     8221 ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt
     8281 tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc
     8341 catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt
     8401 gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata
     8461 gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga
     8521 tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag
     8581 catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa
     8641 aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt
     8701 attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga
     8761 aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct aaattgtaag
     8821 cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa atcagctcat tttttaacca
     8881 ataggccgaa atcggcaaaa tcccttataa atcaaaagaa tagaccgaga tagggttgag
     8941 tgttgttcca gtttggaaca agagtccact attaagaacg tggactccaa cgtcaaaggg
     9001 cgaaaaaccg tctatcaggg cgatggccca ctacgtgaac catcacccta atcaagtttt
     9061 ttggggtcga ggtgccgtaa agcactaaat cggaacccta aagggagccc ccgatttaga
     9121 gcttgacggg gaaagccggc gaacgtggcg agaaaggaag ggaagaaagc gaaaggagcg
     9181 ggcgctaggg cgctggcaag tgtagcggtc acgctgcgcg taaccaccac acccgccgcg
     9241 cttaatgcgc cgctacaggg cgcgtcccat tcgccaatcc gtcggcttga acgaattgtt
     9301 agacattatt tgccgactac cttggtgatc tcgcctttca cgtagtggac aaattcttcc
     9361 aactgatctg cgcgcgaggc caagcgatct tcttcttgtc caagataagc ctgtctagct
     9421 tcaagtatga cgggctgata ctgggccggc aggcgctcca ttgcccagtc ggcagcgaca
     9481 tccttcggcg cgattttgcc ggttactgcg ctgtaccaaa tgcgggacaa cgtaagcact
     9541 acatttcgct catcgccagc ccagtcgggc ggcgagttcc atagcgttaa ggtttcattt
     9601 agcgcctcaa atagatcctg ttcaggaacc ggatcaaaga gttcctccgc cgctggacct
     9661 accaaggcaa cgctatgttc tcttgctttt gtcagcaaga tagccagatc aatgtcgatc
     9721 gtggctggct cgaagatacc tgcaagaatg tcattgcgct gccattctcc aaattgcagt
     9781 tcgcgcttag ctggataacg ccacggaatg atgtcgtcgt gcacaacaat ggtgacttct
     9841 acagcgcgga gaatctcgct ctctccaggg gaagccgaag tttccaaaag gtcgttgatc
     9901 aaagctcgcc gcgttgtttc atcaagcctt acggtcaccg taaccagcaa atcaatatca
     9961 ctgtgtggct tcaggccgcc atccactgcg gagccgtaca aatgtacggc cagcaacgtc
    10021 ggttcgagat ggcgctcgat gacgccaact acctctgata gttgagtcga tacttcggcg
    10081 atcaccgctt ccctcatgat gtttaacttt gttttagggc gactgccctg ctgcgtaaca
    10141 tcgttgctgc tccataacat caaacatcga cccacggcgt aacgcgcttg ctgcttggat
    10201 gcccgaggca tagactgtac cccaaaaaaa cagtcataac aagccatgaa aaccgccact
    10261 gcgccgttac caccgctgcg ttcggtcaag gttctggacc agttgcgtga gcgcatacgc
    10321 tacttaccgg tttattgact accggaagca gtgtgaccgt gtgcttctca aatgcctgag
    10381 gccagtttgc tcaggctctc cccgtggagg taataattga cgatatgatc atttattctg
    10441 cctcccagtt caggctgcaa ataagcgttg atattcagtc aattacaaac attaataacg
    10501 aagagatgac agaaaaattt tcattctgtg acagagaaaa agtagccgaa gatgacggtt
    10561 tgtcacatgg agttggcagg atgtttgatt aaaaacataa caggaagaaa aatgccccgc
    10621 tgtgggcgga caaaatagtt gggaactggg aggggtggaa atggagtttt taaggattat
    10681 ttagggaaga gtgacaaaat agatgggaac tgggtgtagc gtcgtaagct aatacgaaaa
    10741 ttaaaaatga caaaatagtt tggaactaga tttcacttat ctggttcgga tctcctgttt
    10801 aaaccaagtc aatgtcagtg tttttaatta acccgtagtg gctatggcag ggcttgccgc
    10861 cccgacgttg gctgcgagcc ctgggccttc acccgaactt gggggttggg gtggggaaaa
    10921 ggaagaaacg cgggcgtatt ggtcccaatg gggtctcggt ggggtatcga cgtaaccccg
    10981 attgagatat agatttattt aaatggttat gatagttatt gctcagcggt ggcagcagcc
    11041 aactcagctt cctttcgggc tttgttagca gccggatctt ctaggctcaa gcagtgatca
    11101 gatccagaca tgataagata cattgatgag tttggacaaa ccacaactag aatgcagtga
    11161 aaaaaatgct ttatttgtga aatttgtgat gctattgctt tatttgtaac cattataagc
    11221 tgcaataaac aagttaacaa caacaattgc attcatttta tgtttcaggt tcagggggag
    11281 gtgtgggagg ttttttaaag caagtaaaac ctctacaaat gtggtatggc tgattatgat
    11341 cctctagtac ttctcgacaa gcttttatct catccaggct gcccgcccca gccccccaac
    11401 tggaggcatg ccgggtgggg gaatgggcgg gggcacagga ggctgtcctc caggggggac
    11461 ggcaggaggt gggtagctgg ctggcggcag gcccaaccct ggaggaggat gaggggggac
    11521 tgcgtgggta ggcgggaggc gtgggggtgg gggtgggaag ttggggttgt aggtgggtgg
    11581 gggtgggggg tagccaggag tagggggtgg gatggcaggt ggggggaagg aggctggggg
    11641 aggaggcaca ggtggcgggg gcgggttggg cgggtagaca tgggggtggt agggcaggtg
    11701 tgcaggtggg ccgtaggcgg ggggcagggc accgtaggag ggtccctcgg tcttcatcat
    11761 ggactgcagg ctctggtagc cctctccaga catgtaggag ctggtggtgg acatcccggt
    11821 catgtagctg gaggggggtg ggggcggggg ccggtgtggc agcggcggtg gctggggcac
    11881 aggggccggg gggggcgggg gggggatctg ggctttgggg ggggcagtgg catccacagg
    11941 ggccggcggc tcagcctcac ctaaggcagc agcgagggga ggcttccggt ctgggggtgg
    12001 gtgggctgga ggcaacggtg gatgagtggt ctcaattttg gggattt
//
