LOCUS       sequence-463613-       12440 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Protein expression in insect cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12440)
  AUTHORS   Lopez Martinez D, Todorovski I, Noe Gonzalez M, Rusimbi C, Blears D,
            Khallou N, Han Z, Dirac-Svejstrup AB, Svejstrup JQ
  TITLE     PAF1C-mediated activation of CDK12/13 kinase activity is critical 
            for CTD phosphorylation and transcript elongation.
  JOURNAL   Mol Cell. 2025 May 15;85(10):1952-1967.e8. doi: 
            10.1016/j.molcel.2025.04.012. Epub 2025 May 1.
  PUBMED    40315851
REFERENCE   2  (bases 1 to 12440)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2025.04.012"; journalName: "Mol Cell"; date: 
            "2025-05-15- 15"; volume: "85"; issue: "10"; pages: "1952-1967.e8"
COMMENT     Sequence Label: pBIG1e-CDK12deltaRS/Cyclin K
FEATURES             Location/Qualifiers
     source          1..12440
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    2086..2220
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        2376..2467
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     polyA_signal    4382..4516
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     mobile_element  4831..4996
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     CDS             5520..6311
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      6352..6806
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6439..6458)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6648..6669
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        6832..6936
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6937..7005,7006..7797)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7155..7174)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7968..8556
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8457..8476
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8710..8727
                     /label=L4440
                     /note="L4440 vector, forward primer"
     mobile_element  8861..9085
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     CDS             complement(9152..9685)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(join(9874..9879,9880..9896,9897..9902))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     protein_bind    complement(10298..10331)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     complement(10377..10396)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    10421..10469
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     promoter        10564..10655
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     CDS             10701..10724
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             10731..10754
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
ORIGIN
        1 atgaaacagc taatggaagg attggaatac tgtcacaaaa agaatttcct gcatcgggat
       61 attaagtgtt ctaacatttt gctgaataac agtgggcaaa tcaaactagc agattttgga
      121 cttgctcggc tctataactc tgaagagagt cgcccttaca caaacaaagt cattactttg
      181 tggtaccgac ctccagaact actgctagga gaggaacgtt acacaccagc catagatgtt
      241 tggagctgtg gatgtattct tggggaacta ttcacaaaga agcctatttt tcaagccaat
      301 ctggaactgg ctcagctaga actgatcagc cgactttgtg gtagcccttg tccagctgtg
      361 tggcctgatg ttatcaaact gccctacttc aacaccatga aaccgaagaa gcaatatcga
      421 aggcgtctac gagaagaatt ctctttcatt ccttctgcag cacttgattt attggaccac
      481 atgctgacac tagatcctag taagcggtgc acagctgaac agaccctaca gagcgacttc
      541 cttaaagatg tcgaactcag caaaatggct cctccagacc tcccccactg gcaggattgc
      601 catgagttgt ggagtaagaa acggcgacgt cagcgacaaa gtggtgttgt agtcgaagag
      661 ccacctccat ccaaaacttc tcgaaaagaa actacctcag ggacaagtac tgagcctgtg
      721 aagaacagca gcccagcacc acctcagcct gctcctggca aggtggagtc tggggctggg
      781 gatgcaatag gccttgctga catcacacaa cagctgaatc aaagtgaatt ggcagtgtta
      841 ttaaacctgc tgcagagcca aaccgacctg agcatccctc aaatggcaca gctgcttaac
      901 atccactcca acccagagat gcagcagcag ctggaagccc tgaaccaatc catcagtgcc
      961 ctgacggaag ctacttccca gcagcaggac tcagagacca tggccccaga ggagtctttg
     1021 aaggaagcac cctctgcccc agtgatcctg ccttcagcag aacagacgac ccttgaagct
     1081 tcaagcacac cagctgacat gcagaatata ttggcagttc tcttgagtca gctgatgaaa
     1141 acccaagagc cagcaggcag tctggaggaa aacaacagtg acaagaacag tgggccacag
     1201 gggccccgaa gaactcccac aatgccacag gaggaggcag cagcatgtcc tcctcacatt
     1261 cttccaccag agaagaggcc ccctgagccc cccggacctc caccgccgcc acctccaccc
     1321 cctctggttg aaggcgatct ttccagcgcc ccccaggagt tgaacccagc cgtgacagcc
     1381 gccttgctgc aacttttatc ccagcctgaa gcagagcctc ctggccacct gccacatgag
     1441 caccaggcct tgagaccaat ggagtactcc acccgacccc gtccaaacag gacttatgga
     1501 aacactgatg ggcctgaaac agggttcagt gccattgaca ctgatgaacg aaactctggt
     1561 ccagccttga cagaatcctt ggtccagacc ctggtgaaga acaggacctt ctcaggctct
     1621 ctgagccacc ttggggagtc cagcagttac cagggcacag ggtcagtgca gtttccaggg
     1681 gaccaggacc tccgttttgc cagggtcccc ttagcgttac acccggtggt cgggcaacca
     1741 ttcctgaagg ctgagggaag cagcaattct gtggtacatg cagagaccaa attgcaaaac
     1801 tatggggagc tggggccagg aaccactggg gccagcagct caggagcagg ccttcactgg
     1861 gggggcccaa ctcagtcttc tgcttatgga aaactctatc gggggcctac aagagtccca
     1921 ccaagagggg gaagagggag aggagttcct tactaaaagc ttgtcgagaa gtactagagg
     1981 atcataatca gccataccac atttgtagag gttttacttg ctttaaaaaa cctcccacac
     2041 ctccccctga acctgaaaca taaaatgaat gcaattgttg ttgttaactt gtttattgca
     2101 gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt
     2161 tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggatc
     2221 tgatcactgc ttgagcctag aagatccggc tgctaacaaa gcccgaaagg aagctgagtt
     2281 ggctgctgcc accgctgagc aataactatc ataaccattt aaataaactg gatactattg
     2341 cacgtttaaa tcgacctact ccggaatatt aatagatcat ggagataatt aaaatgataa
     2401 ccatctcgca aataaataag tattttactg ttttcgtaac agttttgtaa taaaaaaacc
     2461 tataaatatt ccggattatt cataccgtcc caccatcggg cgcggatcca tgaaggagaa
     2521 taaagaaaat tcaagccctt cagtaacttc agcaaacctg gaccacacaa agccatgttg
     2581 gtactgggat aagaaagact tggctcatac accctcacaa cttgaaggac ttgatccagc
     2641 caccgaggcc cggtaccgcc gagagggcgc tcggttcatc tttgatgtgg gcacacgttt
     2701 ggggctacac tatgataccc tggcaactgg aataatttat tttcatcgct tctatatgtt
     2761 tcattccttc aagcaattcc caagatatgt gacaggagcc tgttgcctct ttctggctgg
     2821 gaaagtagaa gaaacaccaa aaaaatgtaa agatatcatc aaaacagctc gtagtttatt
     2881 aaatgatgta caatttggcc agtttggaga tgacccaaag gaggaagtaa tggttctgga
     2941 gagaatctta ctgcagacca tcaagtttga tttacaggta gaacatccat accagttcct
     3001 actaaaatat gcaaagcaac tcaaaggtga taaaaacaaa attcaaaagt tggttcaaat
     3061 ggcatggaca tttgtaaatg acagtctctg caccaccttg tcactgcagt gggaaccaga
     3121 gatcatagca gtagcagtga tgtatctcgc aggacgtttg tgcaaatttg aaatacaaga
     3181 atggacctcc aaacccatgt ataggagatg gtgggagcag tttgttcaag atgtcccggt
     3241 cgacgttttg gaagacatct gccaccaaat cctggatctt tactcacaag gaaaacaaca
     3301 gatgcctcat cacacccccc atcagctgca acagccccca tctcttcagc ctacaccaca
     3361 agtgccgcaa gtacagcagt cacagccgtc tcaaagctcc gaaccatccc agccccagca
     3421 gaaggacccc cagcaaccag cccagcagca gcagccagcc caacagccca agaaaccctc
     3481 tccgcagccc agttctcccc gacaggttaa gcgagccgtg gttgtttctc ccaaagaaga
     3541 gaacaaagca gcagaaccac caccacctaa aatccccaaa attgagacca ctcatccacc
     3601 gttgcctcca gcccacccac ctccagaccg gaagcctccc ctcgctgctg ccttaggtga
     3661 ggctgagccg ccgggccctg tggatgccac tgacctcccc aaagtccaga ttccccctcc
     3721 ggcccacccg gcccctgtgc accagccacc gccgctgcca caccggcccc cgcccccacc
     3781 cccctccagc tacatgaccg ggatgtccac caccagctcc tacatgtctg gagagggcta
     3841 ccagagcctg cagtccatga tgaagaccga gggaccctcc tacggtgccc tgccccccgc
     3901 ctacggccca cctgcacacc tgccctacca cccccatgtc tacccgccca acccgccccc
     3961 gccacctgtg cctcctcccc cagcctcctt ccccccacct gccatcccac cccctactcc
     4021 tggctacccc ccacccccac ccacctacaa ccccaacttc ccacccccac ccccacgcct
     4081 cccgcctacc cacgcagtcc cccctcatcc tcctccaggg ttgggcctgc cgccagccag
     4141 ctacccacct cctgccgtcc cccctggagg acagcctcct gtgcccccgc ccattccccc
     4201 acccggcatg cctccagttg gggggctggg gcgggcagcc tggatgagat aaaagcttgt
     4261 cgagaagtac tagaggatca taatcagcca taccacattt gtagaggttt tacttgcttt
     4321 aaaaaacctc ccacacctcc ccctgaacct gaaacataaa atgaatgcaa ttgttgttgt
     4381 taacttgttt attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac
     4441 aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc
     4501 ttatcatgtc tggatctgat cactgcttga gcctagaaga tccggctgct aacaaagccc
     4561 gaaaggaagc tgagttggct gctgccaccg ctgagcaata actatcataa ccatttaaat
     4621 aaatctatat ctcaatcggg gttacgtcga taccccaccg agaccccatt gggaccaata
     4681 cgcccgcgtt tcttcctttt ccccacccca acccccaagt tcgggtgaag gcccagggct
     4741 cgcagccaac gtcggggcgg caagccctgc catagccact acgggttaat taaaaacact
     4801 gacattgact tggtttaaac aggagatccg aaccagataa gtgaaatcta gttccaaact
     4861 attttgtcat ttttaatttt cgtattagct tacgacgcta cacccagttc ccatctattt
     4921 tgtcactctt ccctaaataa tccttaaaaa ctccatttcc acccctccca gttcccaact
     4981 attttgtccg cccacagcgg ggcatttttc ttcctgttat gtttttaatc aaacatcctg
     5041 ccaactccat gtgacaaacc gtcatcttcg gctacttttt ctctgtcaca gaatgaaaat
     5101 ttttctgtca tctcttcgtt attaatgttt gtaattgact gaatatcaac gcttatttgc
     5161 agcctgaact gggaggcaga ataaatgatc atatcgtcaa ttattacctc cacggggaga
     5221 gcctgagcaa actggcctca ggcatttgag aagcacacgg tcacactgct tccggtagtc
     5281 aataaaccgg taagtagcgt atgcgctcac gcaactggtc cagaaccttg accgaacgca
     5341 gcggtggtaa cggcgcagtg gcggttttca tggcttgtta tgactgtttt tttggggtac
     5401 agtctatgcc tcgggcatcc aagcagcaag cgcgttacgc cgtgggtcga tgtttgatgt
     5461 tatggagcag caacgatgtt acgcagcagg gcagtcgccc taaaacaaag ttaaacatca
     5521 tgagggaagc ggtgatcgcc gaagtatcga ctcaactatc agaggtagtt ggcgtcatcg
     5581 agcgccatct cgaaccgacg ttgctggccg tacatttgta cggctccgca gtggatggcg
     5641 gcctgaagcc acacagtgat attgatttgc tggttacggt gaccgtaagg cttgatgaaa
     5701 caacgcggcg agctttgatc aacgaccttt tggaaacttc ggcttcccct ggagagagcg
     5761 agattctccg cgctgtagaa gtcaccattg ttgtgcacga cgacatcatt ccgtggcgtt
     5821 atccagctaa gcgcgaactg caatttggag aatggcagcg caatgacatt cttgcaggta
     5881 tcttcgagcc agccacgatc gacattgatc tggctatctt gctgacaaaa gcaagagaac
     5941 atagcgttgc cttggtaggt ccagcggcgg aggaactctt tgatccggtt cctgaacagg
     6001 atctatttga ggcgctaaat gaaaccttaa cgctatggaa ctcgccgccc gactgggctg
     6061 gcgatgagcg aaatgtagtg cttacgttgt cccgcatttg gtacagcgca gtaaccggca
     6121 aaatcgcgcc gaaggatgtc gctgccgact gggcaatgga gcgcctgccg gcccagtatc
     6181 agcccgtcat acttgaagct agacaggctt atcttggaca agaagaagat cgcttggcct
     6241 cgcgcgcaga tcagttggaa gaatttgtcc actacgtgaa aggcgagatc accaaggtag
     6301 tcggcaaata atgtctaaca attcgttcaa gccgacggat tggcgaatgg gacgcgccct
     6361 gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg
     6421 ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg
     6481 gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac
     6541 ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct
     6601 gatagacggt ttttcgccct ttgacgttgg agtccacgtt cttaatagtg gactcttgtt
     6661 ccaaactgga acaacactca accctatctc ggtctattct tttgatttat aagggatttt
     6721 gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaattt
     6781 taacaaaata ttaacgctta caatttaggt ggcacttttc ggggaaatgt gcgcggaacc
     6841 cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc
     6901 tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc
     6961 gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg
     7021 gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat
     7081 ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc
     7141 acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa
     7201 ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa
     7261 aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt
     7321 gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct
     7381 tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat
     7441 gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg
     7501 cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg
     7561 atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt
     7621 attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg
     7681 ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg
     7741 gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg
     7801 tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa
     7861 aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt
     7921 tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt
     7981 tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt
     8041 ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag
     8101 ataccaaata ctattcttct agtgtagccg tagttaggcc accacttcaa gaactctgta
     8161 gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat
     8221 aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg
     8281 ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg
     8341 agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac
     8401 aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga
     8461 aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt
     8521 ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta
     8581 cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat
     8641 tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg
     8701 accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc
     8761 cttacgcatc tgtgcggtat ttcacaccgc atagaccagc cgcgtaacct ggcaaaatcg
     8821 gttacggttg agtaataaat ggatgccctg cgtaagcggg tgtgggcgga caataaagtc
     8881 ttaaactgaa caaaatagat ctaaactatg acaataaagt cttaaactag acagaatagt
     8941 tgtaaactga aatcagtcca gttatgctgt gaaaaagcat actggacttt tgttatggct
     9001 aaagcaaact cttcattttc tgaagtgcaa attgcccgtc gtattaaaga ggggcgtggc
     9061 caagggcatg gtaaagacta tattcgcggc gttgtgacaa tttaccgaac aactccgcgg
     9121 ccgggaagcc gatctcggct tgaacgaatt gttaggtggc ggtacttggg tcgatatcaa
     9181 agtgcatcac ttcttcccgt atgcccaact ttgtatagag agccactgcg ggatcgtcac
     9241 cgtaatctgc ttgcacgtag atcacataag caccaagcgc gttggcctca tgcttgagga
     9301 gattgatgag cgcggtggca atgccctgcc tccggtgctc gccggagact gcgagatcat
     9361 agatatagat ctcactacgc ggctgctcaa acttgggcag aacgtaagcc gcgagagcgc
     9421 caacaaccgc ttcttggtcg aaggcagcaa gcgcgatgaa tgtcttacta cggagcaagt
     9481 tcccgaggta atcggagtcc ggctgatgtt gggagtaggt ggctacgtct ccgaactcac
     9541 gaccgaaaag atcaagagca gcccgcatgg atttgacttg gtcagggccg agcctacatg
     9601 tgcgaatgat gcccatactt gagccaccta actttgtttt agggcgactg ccctgctgcg
     9661 taacatcgtt gctgctgcgt aacatcgttg ctgctccata acatcaaaca tcgacccacg
     9721 gcgtaacgcg cttgctgctt ggatgcccga ggcatagact gtacaaaaaa acagtcataa
     9781 caagccatga aaaccgccac tgcgccgtta ccaccgctgc gttcggtcaa ggttctggac
     9841 cagttgcgtg agcgcatacg ctacttgcat tacagtttac gaaccgaaca ggcttatgtc
     9901 aactgggttt gtgccttcat ccgtttccac ggtgtgcgtc acccggcaac cttgggcagc
     9961 agcgaagtcg aggcatttct gtcctggctg gcgaacgagc gcaaggtttc ggtctccacg
    10021 catcgtcagg cattggcggc cttgctgttc ttctacggca aggtgctgtg cacggatctg
    10081 cccttgcttc aggagatcgg tagacctcgg ccgtcgcggc gcttgccggt ggtgctgacc
    10141 ccggatgaag tggttcgcat cctcggtttt ctggaaggcg agcatcgttt gttcgcccag
    10201 gactctagct atagttctag tggttggcta cagctttgtt tgtactatca acaggttgaa
    10261 ctgctgatca acagatcctc tacgcggccg cggtaccata acttcgtata gcatacatta
    10321 tacgaagtta tctgggttta aacggttcac atagcttagt ttttatcgag gctaactgaa
    10381 acacggaagg agacaatacc ggaaggaacc cgcgctatga cggcaataaa aagacagaat
    10441 aaaacgcacg ggtgttgggt cgtttgttca taaacgcggg gttcggtccc agggctggca
    10501 ctctgtcaag tgaacgctct atggtctaaa gatttaaatc gacctactcc ggaatattaa
    10561 tagatcatgg agataattaa aatgataacc atctcgcaaa taaataagta ttttactgtt
    10621 ttcgtaacag ttttgtaata aaaaaaccta taaatattcc ggattattca taccgtccca
    10681 ccatcgggcg cggatccatg gactacaagg acgacgatga caagctcgat cttgaagtcc
    10741 tctttcaggg acccatgccc aattcagaga gacatggggg caagaaggac gggagtggag
    10801 gagcttctgg aactttgcag ccgtcatcgg gaggcggcag ctctaacagc agagagcgtc
    10861 accgcttggt atcgaagcac aagcggcata agtccaaaca ctccaaagac atggggttgg
    10921 tgacccccga agcagcatcc ctgggcacag ttatcaaacc tttggtggag tatgatgata
    10981 tcagctctga ttccgacacc ttctccgatg acatggcctt caaactagac cgaagggaga
    11041 acgacgaacg tcgtggatca gatcattcca gtatctcacc tgtcaggctt ccacttaatt
    11101 ccagtctggg agctgaactc agtaggaaaa agaaggaaag agcagctgct gctgctgcag
    11161 caaagatgga tggaaaggag tccaagggtt cacctgtatt tttgcctaga aaagagaaca
    11221 gttcagtaga ggctaaggat tcaggtttgg agtctaaaaa gttacccaga agtgtaaaat
    11281 tggaaaaatc tgccccagat actgaactgg tgaatgtaac acatctaaac acagaggtaa
    11341 aaaattcttc agatacaggg aaagtaaagt tggatgagaa ctccgagaag catcttgtta
    11401 aagatttgaa agcacaggga acaagagact ctaaacccat agcactgaaa gaggagattg
    11461 ttactccaaa ggagacagaa acatcagaaa aggagacccc tccacctctt cccacaattg
    11521 cttctccccc accccctcta ccaactacta cccctccacc tcagacaccc cctttgccac
    11581 ctttgcctcc aataccagct cttccacagc aaccacctct gcctccttct cagccagcat
    11641 ttagtcaggt tcctgcttcc agtacttcaa ctttgccccc ttctactcac tcaaagacat
    11701 ctgctgtgtc ctctcaggca aattctcagc cccctgtaca ggtttctgtg aagactcaag
    11761 tatctgtaac agctgctatt ccacacctga aaacttcaac gttgcctcct ttgcccctcc
    11821 cacccttatt acctggagat gatgacatgg atagtccaaa agaaactctt ccttcaaaac
    11881 ctgtgaagaa agagaaggaa cagaggacac gtcacttact cacagacctt cctctccctc
    11941 cagagctccc tggtggagat ctgtctcccc cagactctcc agaaccaaag gcaatcacac
    12001 cacctcagca accatataaa aagagaccaa aaatttgttg tcctcgttat ggagaaagaa
    12061 gacaaacaga aagcgactgg gggaaacgct gtgtggacaa gtttgacatt attgggatta
    12121 ttggagaagg aacctatggc caagtatata aagccaagga caaagacaca ggagaactag
    12181 tggctctgaa gaaggtgaga ctagacaatg agaaagaggg cttcccaatc acagccattc
    12241 gtgaaatcaa aatccttcgt cagttaatcc accgaagtgt tgttaacatg aaggaaattg
    12301 tcacagataa acaagatgca ctggatttca agaaggacaa aggtgccttt taccttgtat
    12361 ttgagtatat ggaccatgac ttaatgggac tgctagaatc tggtttggtg cacttttctg
    12421 aggaccatat caagtcgttc
//
