LOCUS       sequence-463616-       13271 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Protein expression in insect cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13271)
  AUTHORS   Lopez Martinez D, Todorovski I, Noe Gonzalez M, Rusimbi C, Blears D,
            Khallou N, Han Z, Dirac-Svejstrup AB, Svejstrup JQ
  TITLE     PAF1C-mediated activation of CDK12/13 kinase activity is critical 
            for CTD phosphorylation and transcript elongation.
  JOURNAL   Mol Cell. 2025 May 15;85(10):1952-1967.e8. doi: 
            10.1016/j.molcel.2025.04.012. Epub 2025 May 1.
  PUBMED    40315851
REFERENCE   2  (bases 1 to 13271)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2025.04.012"; journalName: "Mol Cell"; date: 
            "2025-05-15- 15"; volume: "85"; issue: "10"; pages: "1952-1967.e8"
COMMENT     Sequence Label: pBIG1e-CDK12/Cyclin K
FEATURES             Location/Qualifiers
     source          1..13271
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(join(673..678,679..695,696..701))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     protein_bind    complement(1097..1130)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     complement(1176..1195)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    1220..1268
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     promoter        1363..1454
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     CDS             1500..1523
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             1530..1553
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     polyA_signal    6156..6290
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        6446..6537
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     polyA_signal    8452..8586
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     mobile_element  8901..9066
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     CDS             9590..10381
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      10422..10876
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(10509..10528)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     10718..10739
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        10902..11006
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(11007..11075,11076..11867)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(11225..11244)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      12038..12626
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     12527..12546
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     12780..12797
                     /label=L4440
                     /note="L4440 vector, forward primer"
     mobile_element  12931..13155
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     CDS             complement(join(13222..13271,1..484))
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
ORIGIN
        1 tgcccaactt tgtatagaga gccactgcgg gatcgtcacc gtaatctgct tgcacgtaga
       61 tcacataagc accaagcgcg ttggcctcat gcttgaggag attgatgagc gcggtggcaa
      121 tgccctgcct ccggtgctcg ccggagactg cgagatcata gatatagatc tcactacgcg
      181 gctgctcaaa cttgggcaga acgtaagccg cgagagcgcc aacaaccgct tcttggtcga
      241 aggcagcaag cgcgatgaat gtcttactac ggagcaagtt cccgaggtaa tcggagtccg
      301 gctgatgttg ggagtaggtg gctacgtctc cgaactcacg accgaaaaga tcaagagcag
      361 cccgcatgga tttgacttgg tcagggccga gcctacatgt gcgaatgatg cccatacttg
      421 agccacctaa ctttgtttta gggcgactgc cctgctgcgt aacatcgttg ctgctgcgta
      481 acatcgttgc tgctccataa catcaaacat cgacccacgg cgtaacgcgc ttgctgcttg
      541 gatgcccgag gcatagactg tacaaaaaaa cagtcataac aagccatgaa aaccgccact
      601 gcgccgttac caccgctgcg ttcggtcaag gttctggacc agttgcgtga gcgcatacgc
      661 tacttgcatt acagtttacg aaccgaacag gcttatgtca actgggtttg tgccttcatc
      721 cgtttccacg gtgtgcgtca cccggcaacc ttgggcagca gcgaagtcga ggcatttctg
      781 tcctggctgg cgaacgagcg caaggtttcg gtctccacgc atcgtcaggc attggcggcc
      841 ttgctgttct tctacggcaa ggtgctgtgc acggatctgc ccttgcttca ggagatcggt
      901 agacctcggc cgtcgcggcg cttgccggtg gtgctgaccc cggatgaagt ggttcgcatc
      961 ctcggttttc tggaaggcga gcatcgtttg ttcgcccagg actctagcta tagttctagt
     1021 ggttggctac agctttgttt gtactatcaa caggttgaac tgctgatcaa cagatcctct
     1081 acgcggccgc ggtaccataa cttcgtatag catacattat acgaagttat ctgggtttaa
     1141 acggttcaca tagcttagtt tttatcgagg ctaactgaaa cacggaagga gacaataccg
     1201 gaaggaaccc gcgctatgac ggcaataaaa agacagaata aaacgcacgg gtgttgggtc
     1261 gtttgttcat aaacgcgggg ttcggtccca gggctggcac tctgtcaagt gaacgctcta
     1321 tggtctaaag atttaaatcg acctactccg gaatattaat agatcatgga gataattaaa
     1381 atgataacca tctcgcaaat aaataagtat tttactgttt tcgtaacagt tttgtaataa
     1441 aaaaacctat aaatattccg gattattcat accgtcccac catcgggcgc ggatccatgg
     1501 actacaagga cgacgatgac aagctcgatc ttgaagtcct ctttcaggga cccatgccca
     1561 attcagagag acatgggggc aagaaggacg ggagtggagg agcttctgga actttgcagc
     1621 cgtcatcggg aggcggcagc tctaacagca gagagcgtca ccgcttggta tcgaagcaca
     1681 agcggcataa gtccaaacac tccaaagaca tggggttggt gacccccgaa gcagcatccc
     1741 tgggcacagt tatcaaacct ttggtggagt atgatgatat cagctctgat tccgacacct
     1801 tctccgatga catggccttc aaactagacc gaagggagaa cgacgaacgt cgtggatcag
     1861 atcggagcga ccgcctgcac aaacatcgtc accaccagca caggcgttcc cgggacttac
     1921 taaaagctaa acagaccgaa aaagaaaaaa gccaagaagt ctccagcaag tcgggatcga
     1981 tgaaggaccg gatatcggga agttcaaagc gttcgaatga ggagactgat gactatggga
     2041 aggcgcaggt agccaaaagc agcagcaagg aatccaggtc atccaagctc cacaaggaga
     2101 agaccaggaa agaacgggag ctgaagtctg ggcacaaaga ccggagtaaa agtcatcgaa
     2161 aaagggaaac acccaaaagt tacaaaacag tggacagccc aaaacggaga tccaggagcc
     2221 cccacaggaa gtggtctgac agctccaaac aagatgatag cccctcggga gcttcttatg
     2281 gccaagatta tgaccttagt ccctcacgat ctcatacctc gagcaattat gactcctaca
     2341 agaaaagtcc tggaagtacc tcgagaaggc agtcggtcag tcccccttac aaggagcctt
     2401 cggcctacca gtccagcacc cggtcaccga gcccctacag taggcgacag agatctgtca
     2461 gtccctatag caggagacgg tcgtccagct acgaaagaag tggctcttac agcgggcgat
     2521 cgcccagtcc ctatggtcga aggcggtcca gcagcccttt cctgagcaag cggtctctga
     2581 gtcggagtcc actccccagt aggaaatcca tgaagtccag aagtagaagt cctgcatatt
     2641 caagacattc atcttctcat agtaaaaaga agagatccag ttcacgcagt cgtcattcca
     2701 gtatctcacc tgtcaggctt ccacttaatt ccagtctggg agctgaactc agtaggaaaa
     2761 agaaggaaag agcagctgct gctgctgcag caaagatgga tggaaaggag tccaagggtt
     2821 cacctgtatt tttgcctaga aaagagaaca gttcagtaga ggctaaggat tcaggtttgg
     2881 agtctaaaaa gttacccaga agtgtaaaat tggaaaaatc tgccccagat actgaacttg
     2941 tgaatgtaac acatctaaac acagaggtaa aaaattcttc agatacaggg aaagtaaagt
     3001 tggatgagaa ctccgagaag catcttgtta aagatttgaa agcacaggga acaagagact
     3061 ctaaacccat agcactgaaa gaggagattg ttactccaaa ggagacagaa acatcagaaa
     3121 aggagacccc tccacctctt cccacaattg cttctccccc accccctcta ccaactacta
     3181 cccctccacc tcagacaccc cctttgccac ctttgcctcc aataccagct cttccacagc
     3241 aaccacctct gcctccttct cagccagcat ttagtcaggt tcctgcttcc agtacttcaa
     3301 ctttgccccc ttctactcac tcaaagacat ctgctgtgtc ctctcaggca aattctcagc
     3361 cccctgtaca ggtttctgtg aagactcaag tatctgtaac agctgctatt ccacacctga
     3421 aaacttcaac gttgcctcct ttgcccctcc cacccttatt acctggagat gatgacatgg
     3481 atagtccaaa agaaactctt ccttcaaaac ctgtgaagaa agagaaggaa cagaggacac
     3541 gtcacttact cacagacctt cctctccctc cagagctccc tggtggagat ctgtctcccc
     3601 cagactctcc agaaccaaag gcaatcacac cacctcagca accatataaa aagagaccaa
     3661 aaatttgttg tcctcgttat ggagaaagaa gacaaacaga aagcgactgg gggaaacgct
     3721 gtgtggacaa gtttgacatt attgggatta ttggagaagg aacctatggc caagtatata
     3781 aagccaagga caaagacaca ggagaactag tggctctgaa gaaggtgaga ctagacaatg
     3841 agaaagaggg cttcccaatc acagccattc gtgaaatcaa aatccttcgt cagttaatcc
     3901 accgaagtgt tgttaacatg aaggaaattg tcacagataa acaagatgca ctggatttca
     3961 agaaggacaa aggtgccttt taccttgtat ttgagtatat ggaccatgac ttaatgggac
     4021 tgctagaatc tggtttggtg cacttttctg aggaccatat caagtcgttc atgaaacagc
     4081 taatggaagg attggaatac tgtcacaaaa agaatttcct gcatcgggat attaagtgtt
     4141 ctaacatttt gctgaataac agtgggcaaa tcaaactagc agattttgga cttgctcggc
     4201 tctataactc tgaagagagt cgcccttaca caaacaaagt cattactttg tggtaccgac
     4261 ctccagaact actgctagga gaggaacgtt acacaccagc catagatgtt tggagctgtg
     4321 gatgtattct tggggaacta ttcacaaaga agcctatttt tcaagccaat ctggaactgg
     4381 ctcagctaga actgatcagc cgactttgtg gtagcccttg tccagctgtg tggcctgatg
     4441 ttatcaaact gccctacttc aacaccatga aaccgaagaa gcaatatcga aggcgtctac
     4501 gagaagaatt ctctttcatt ccttctgcag cacttgattt attggaccac atgctgacac
     4561 tagatcctag taagcggtgc acagctgaac agaccctaca gagcgacttc cttaaagatg
     4621 tcgaactcag caaaatggct cctccagacc tcccccactg gcaggattgc catgagttgt
     4681 ggagtaagaa acggcgacgt cagcgacaaa gtggtgttgt agtcgaagag ccacctccat
     4741 ccaaaacttc tcgaaaagaa actacctcag ggacaagtac tgagcctgtg aagaacagca
     4801 gcccagcacc acctcagcct gctcctggca aggtggagtc tggggctggg gatgcaatag
     4861 gccttgctga catcacacaa cagctgaatc aaagtgaatt ggcagtgtta ttaaacctgc
     4921 tgcagagcca aaccgacctg agcatccctc aaatggcaca gctgcttaac atccactcca
     4981 acccagagat gcagcagcag ctggaagccc tgaaccaatc catcagtgcc ctgacggaag
     5041 ctacttccca gcagcaggac tcagagacca tggccccaga ggagtctttg aaggaagcac
     5101 cctctgcccc agtgatcctg ccttcagcag aacagacgac ccttgaagct tcaagcacac
     5161 cagctgacat gcagaatata ttggcagttc tcttgagtca gctgatgaaa acccaagagc
     5221 cagcaggcag tctggaggaa aacaacagtg acaagaacag tgggccacag gggccccgaa
     5281 gaactcccac aatgccacag gaggaggcag cagcatgtcc tcctcacatt cttccaccag
     5341 agaagaggcc ccctgagccc cccggacctc caccgccgcc acctccaccc cctctggttg
     5401 aaggcgatct ttccagcgcc ccccaggagt tgaacccagc cgtgacagcc gccttgctgc
     5461 aacttttatc ccagcctgaa gcagagcctc ctggccacct gccacatgag caccaggcct
     5521 tgagaccaat ggagtactcc acccgacccc gtccaaacag gacttatgga aacactgatg
     5581 ggcctgaaac agggttcagt gccattgaca ctgatgaacg aaactctggt ccagccttga
     5641 cagaatcctt ggtccagacc ctggtgaaga acaggacctt ctcaggctct ctgagccacc
     5701 ttggggagtc cagcagttac cagggcacag ggtcagtgca gtttccaggg gaccaggacc
     5761 tccgttttgc cagggtcccc ttagcgttac acccggtggt cgggcaacca ttcctgaagg
     5821 ctgagggaag cagcaattct gtggtacatg cagagaccaa attgcaaaac tatggggagc
     5881 tggggccagg aaccactggg gccagcagct caggagcagg ccttcactgg gggggcccaa
     5941 ctcagtcttc tgcttatgga aaactctatc gggggcctac aagagtccca ccaagagggg
     6001 gaagagggag aggagttcct tactaaaagc ttgtcgagaa gtactagagg atcataatca
     6061 gccataccac atttgtagag gttttacttg ctttaaaaaa cctcccacac ctccccctga
     6121 acctgaaaca taaaatgaat gcaattgttg ttgttaactt gtttattgca gcttataatg
     6181 gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt
     6241 ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggatc tgatcactgc
     6301 ttgagcctag aagatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc
     6361 accgctgagc aataactatc ataaccattt aaataaactg gatactattg cacgtttaaa
     6421 tcgacctact ccggaatatt aatagatcat ggagataatt aaaatgataa ccatctcgca
     6481 aataaataag tattttactg ttttcgtaac agttttgtaa taaaaaaacc tataaatatt
     6541 ccggattatt cataccgtcc caccatcggg cgcggatcca tgaaggagaa taaagaaaat
     6601 tcaagccctt cagtaacttc agcaaacctg gaccacacaa agccatgttg gtactgggat
     6661 aagaaagact tggctcatac accctcacaa cttgaaggac ttgatccagc caccgaggcc
     6721 cggtaccgcc gagagggcgc tcggttcatc tttgatgtgg gcacacgttt ggggctacac
     6781 tatgataccc tggcaactgg aataatttat tttcatcgct tctatatgtt tcattccttc
     6841 aagcaattcc caagatatgt gacaggagcc tgttgcctct ttctggctgg gaaagtagaa
     6901 gaaacaccaa aaaaatgtaa agatatcatc aaaacagctc gtagtttatt aaatgatgta
     6961 caatttggcc agtttggaga tgacccaaag gaggaagtaa tggttctgga gagaatctta
     7021 ctgcagacca tcaagtttga tttacaggta gaacatccat accagttcct actaaaatat
     7081 gcaaagcaac tcaaaggtga taaaaacaaa attcaaaagt tggttcaaat ggcatggaca
     7141 tttgtaaatg acagtctctg caccaccttg tcactgcagt gggaaccaga gatcatagca
     7201 gtagcagtga tgtatctcgc aggacgtttg tgcaaatttg aaatacaaga atggacctcc
     7261 aaacccatgt ataggagatg gtgggagcag tttgttcaag atgtcccggt cgacgttttg
     7321 gaagacatct gccaccaaat cctggatctt tactcacaag gaaaacaaca gatgcctcat
     7381 cacacccccc atcagctgca acagccccca tctcttcagc ctacaccaca agtgccgcaa
     7441 gtacagcagt cacagccgtc tcaaagctcc gaaccatccc agccccagca gaaggacccc
     7501 cagcaaccag cccagcagca gcagccagcc caacagccca agaaaccctc tccgcagccc
     7561 agttctcccc gacaggttaa gcgagccgtg gttgtttctc ccaaagaaga gaacaaagca
     7621 gcagaaccac caccacctaa aatccccaaa attgagacca ctcatccacc gttgcctcca
     7681 gcccacccac ctccagaccg gaagcctccc ctcgctgctg ccttaggtga ggctgagccg
     7741 ccgggccctg tggatgccac tgacctcccc aaagtccaga ttccccctcc ggcccacccg
     7801 gcccctgtgc accagccacc gccgctgcca caccggcccc cgcccccacc cccctccagc
     7861 tacatgaccg ggatgtccac caccagctcc tacatgtctg gagagggcta ccagagcctg
     7921 cagtccatga tgaagaccga gggaccctcc tacggtgccc tgccccccgc ctacggccca
     7981 cctgcacacc tgccctacca cccccatgtc tacccgccca acccgccccc gccacctgtg
     8041 cctcctcccc cagcctcctt ccccccacct gccatcccac cccctactcc tggctacccc
     8101 ccacccccac ccacctacaa ccccaacttc ccacccccac ccccacgcct cccgcctacc
     8161 cacgcagtcc cccctcatcc tcctccaggg ttgggcctgc cgccagccag ctacccacct
     8221 cctgccgtcc cccctggagg acagcctcct gtgcccccgc ccattccccc acccggcatg
     8281 cctccagttg gggggctggg gcgggcagcc tggatgagat aaaagcttgt cgagaagtac
     8341 tagaggatca taatcagcca taccacattt gtagaggttt tacttgcttt aaaaaacctc
     8401 ccacacctcc ccctgaacct gaaacataaa atgaatgcaa ttgttgttgt taacttgttt
     8461 attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca
     8521 tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc
     8581 tggatctgat cactgcttga gcctagaaga tccggctgct aacaaagccc gaaaggaagc
     8641 tgagttggct gctgccaccg ctgagcaata actatcataa ccatttaaat aaatctatat
     8701 ctcaatcggg gttacgtcga taccccaccg agaccccatt gggaccaata cgcccgcgtt
     8761 tcttcctttt ccccacccca acccccaagt tcgggtgaag gcccagggct cgcagccaac
     8821 gtcggggcgg caagccctgc catagccact acgggttaat taaaaacact gacattgact
     8881 tggtttaaac aggagatccg aaccagataa gtgaaatcta gttccaaact attttgtcat
     8941 ttttaatttt cgtattagct tacgacgcta cacccagttc ccatctattt tgtcactctt
     9001 ccctaaataa tccttaaaaa ctccatttcc acccctccca gttcccaact attttgtccg
     9061 cccacagcgg ggcatttttc ttcctgttat gtttttaatc aaacatcctg ccaactccat
     9121 gtgacaaacc gtcatcttcg gctacttttt ctctgtcaca gaatgaaaat ttttctgtca
     9181 tctcttcgtt attaatgttt gtaattgact gaatatcaac gcttatttgc agcctgaact
     9241 gggaggcaga ataaatgatc atatcgtcaa ttattacctc cacggggaga gcctgagcaa
     9301 actggcctca ggcatttgag aagcacacgg tcacactgct tccggtagtc aataaaccgg
     9361 taagtagcgt atgcgctcac gcaactggtc cagaaccttg accgaacgca gcggtggtaa
     9421 cggcgcagtg gcggttttca tggcttgtta tgactgtttt tttggggtac agtctatgcc
     9481 tcgggcatcc aagcagcaag cgcgttacgc cgtgggtcga tgtttgatgt tatggagcag
     9541 caacgatgtt acgcagcagg gcagtcgccc taaaacaaag ttaaacatca tgagggaagc
     9601 ggtgatcgcc gaagtatcga ctcaactatc agaggtagtt ggcgtcatcg agcgccatct
     9661 cgaaccgacg ttgctggccg tacatttgta cggctccgca gtggatggcg gcctgaagcc
     9721 acacagtgat attgatttgc tggttacggt gaccgtaagg cttgatgaaa caacgcggcg
     9781 agctttgatc aacgaccttt tggaaacttc ggcttcccct ggagagagcg agattctccg
     9841 cgctgtagaa gtcaccattg ttgtgcacga cgacatcatt ccgtggcgtt atccagctaa
     9901 gcgcgaactg caatttggag aatggcagcg caatgacatt cttgcaggta tcttcgagcc
     9961 agccacgatc gacattgatc tggctatctt gctgacaaaa gcaagagaac atagcgttgc
    10021 cttggtaggt ccagcggcgg aggaactctt tgatccggtt cctgaacagg atctatttga
    10081 ggcgctaaat gaaaccttaa cgctatggaa ctcgccgccc gactgggctg gcgatgagcg
    10141 aaatgtagtg cttacgttgt cccgcatttg gtacagcgca gtaaccggca aaatcgcgcc
    10201 gaaggatgtc gctgccgact gggcaatgga gcgcctgccg gcccagtatc agcccgtcat
    10261 acttgaagct agacaggctt atcttggaca agaagaagat cgcttggcct cgcgcgcaga
    10321 tcagttggaa gaatttgtcc actacgtgaa aggcgagatc accaaggtag tcggcaaata
    10381 atgtctaaca attcgttcaa gccgacggat tggcgaatgg gacgcgccct gtagcggcgc
    10441 attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct
    10501 agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg
    10561 tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga
    10621 ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt
    10681 ttttcgccct ttgacgttgg agtccacgtt cttaatagtg gactcttgtt ccaaactgga
    10741 acaacactca accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg
    10801 gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata
    10861 ttaacgctta caatttaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt
    10921 tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc
    10981 ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc
    11041 ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa
    11101 aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg
    11161 gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag
    11221 ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc
    11281 gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta
    11341 cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg
    11401 cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca
    11461 acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac
    11521 caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat
    11581 taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg
    11641 ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata
    11701 aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta
    11761 agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa
    11821 atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag
    11881 tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg
    11941 tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact
    12001 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
    12061 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc
    12121 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata
    12181 ctattcttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta
    12241 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc
    12301 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg
    12361 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac
    12421 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg
    12481 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt
    12541 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct
    12601 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg
    12661 ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata
    12721 accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca
    12781 gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc
    12841 tgtgcggtat ttcacaccgc atagaccagc cgcgtaacct ggcaaaatcg gttacggttg
    12901 agtaataaat ggatgccctg cgtaagcggg tgtgggcgga caataaagtc ttaaactgaa
    12961 caaaatagat ctaaactatg acaataaagt cttaaactag acagaatagt tgtaaactga
    13021 aatcagtcca gttatgctgt gaaaaagcat actggacttt tgttatggct aaagcaaact
    13081 cttcattttc tgaagtgcaa attgcccgtc gtattaaaga ggggcgtggc caagggcatg
    13141 gtaaagacta tattcgcggc gttgtgacaa tttaccgaac aactccgcgg ccgggaagcc
    13201 gatctcggct tgaacgaatt gttaggtggc ggtacttggg tcgatatcaa agtgcatcac
    13261 ttcttcccgt a
//
