LOCUS       sequence-463618-       18401 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Protein expression in insect cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 18401)
  AUTHORS   Lopez Martinez D, Todorovski I, Noe Gonzalez M, Rusimbi C, Blears D,
            Khallou N, Han Z, Dirac-Svejstrup AB, Svejstrup JQ
  TITLE     PAF1C-mediated activation of CDK12/13 kinase activity is critical 
            for CTD phosphorylation and transcript elongation.
  JOURNAL   Mol Cell. 2025 May 15;85(10):1952-1967.e8. doi: 
            10.1016/j.molcel.2025.04.012. Epub 2025 May 1.
  PUBMED    40315851
REFERENCE   2  (bases 1 to 18401)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2025.04.012"; journalName: "Mol Cell"; date: 
            "2025-05-15- 15"; volume: "85"; issue: "10"; pages: "1952-1967.e8"
COMMENT     Sequence Label: pBIG1a-PAF1C(CTR9/CDC73/PAF1/LEO1/WDR61)
FEATURES             Location/Qualifiers
     source          1..18401
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             442..1233
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      1274..1728
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1361..1380)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1570..1591
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        1754..1858
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1859..1927,1928..2719)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2077..2096)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2890..3478
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3379..3398
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3632..3649
                     /label=L4440
                     /note="L4440 vector, forward primer"
     mobile_element  3783..4007
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     CDS             complement(4074..4607)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(join(4796..4801,4802..4818,4819..4824))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     protein_bind    complement(5220..5253)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     complement(5299..5318)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    5343..5391
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     promoter        5486..5577
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     CDS             9145..9168
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             9169..9192
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     polyA_signal    9325..9459
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        9615..9706
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     CDS             9752..9775
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             9782..9805
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     polyA_signal    11531..11665
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        11821..11912
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     polyA_signal    14085..14219
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        14375..14466
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     polyA_signal    16234..16368
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        16524..16615
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     polyA_signal    17705..17839
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     mobile_element  18154..18319
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
ORIGIN
        1 ttctctgtca cagaatgaaa atttttctgt catctcttcg ttattaatgt ttgtaattga
       61 ctgaatatca acgcttattt gcagcctgaa ctgggaggca gaataaatga tcatatcgtc
      121 aattattacc tccacgggga gagcctgagc aaactggcct caggcatttg agaagcacac
      181 ggtcacactg cttccggtag tcaataaacc ggtaagtagc gtatgcgctc acgcaactgg
      241 tccagaacct tgaccgaacg cagcggtggt aacggcgcag tggcggtttt catggcttgt
      301 tatgactgtt tttttggggt acagtctatg cctcgggcat ccaagcagca agcgcgttac
      361 gccgtgggtc gatgtttgat gttatggagc agcaacgatg ttacgcagca gggcagtcgc
      421 cctaaaacaa agttaaacat catgagggaa gcggtgatcg ccgaagtatc gactcaacta
      481 tcagaggtag ttggcgtcat cgagcgccat ctcgaaccga cgttgctggc cgtacatttg
      541 tacggctccg cagtggatgg cggcctgaag ccacacagtg atattgattt gctggttacg
      601 gtgaccgtaa ggcttgatga aacaacgcgg cgagctttga tcaacgacct tttggaaact
      661 tcggcttccc ctggagagag cgagattctc cgcgctgtag aagtcaccat tgttgtgcac
      721 gacgacatca ttccgtggcg ttatccagct aagcgcgaac tgcaatttgg agaatggcag
      781 cgcaatgaca ttcttgcagg tatcttcgag ccagccacga tcgacattga tctggctatc
      841 ttgctgacaa aagcaagaga acatagcgtt gccttggtag gtccagcggc ggaggaactc
      901 tttgatccgg ttcctgaaca ggatctattt gaggcgctaa atgaaacctt aacgctatgg
      961 aactcgccgc ccgactgggc tggcgatgag cgaaatgtag tgcttacgtt gtcccgcatt
     1021 tggtacagcg cagtaaccgg caaaatcgcg ccgaaggatg tcgctgccga ctgggcaatg
     1081 gagcgcctgc cggcccagta tcagcccgtc atacttgaag ctagacaggc ttatcttgga
     1141 caagaagaag atcgcttggc ctcgcgcgca gatcagttgg aagaatttgt ccactacgtg
     1201 aaaggcgaga tcaccaaggt agtcggcaaa taatgtctaa caattcgttc aagccgacgg
     1261 attggcgaat gggacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg
     1321 cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct
     1381 tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta
     1441 gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt
     1501 tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg
     1561 ttcttaatag tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt
     1621 cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat gagctgattt
     1681 aacaaaaatt taacgcgaat tttaacaaaa tattaacgct tacaatttag gtggcacttt
     1741 tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta
     1801 tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat
     1861 gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt
     1921 ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg
     1981 agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga
     2041 agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg
     2101 tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt
     2161 tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg
     2221 cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg
     2281 aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga
     2341 tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc
     2401 tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc
     2461 ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc
     2521 ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg
     2581 cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac
     2641 gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc
     2701 actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt
     2761 aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac
     2821 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa
     2881 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
     2941 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
     3001 aactggcttc agcagagcgc agataccaaa tactattctt ctagtgtagc cgtagttagg
     3061 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
     3121 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
     3181 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
     3241 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct
     3301 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
     3361 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
     3421 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
     3481 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt
     3541 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
     3601 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
     3661 gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatagacca
     3721 gccgcgtaac ctggcaaaat cggttacggt tgagtaataa atggatgccc tgcgtaagcg
     3781 ggtgtgggcg gacaataaag tcttaaactg aacaaaatag atctaaacta tgacaataaa
     3841 gtcttaaact agacagaata gttgtaaact gaaatcagtc cagttatgct gtgaaaaagc
     3901 atactggact tttgttatgg ctaaagcaaa ctcttcattt tctgaagtgc aaattgcccg
     3961 tcgtattaaa gaggggcgtg gccaagggca tggtaaagac tatattcgcg gcgttgtgac
     4021 aatttaccga acaactccgc ggccgggaag ccgatctcgg cttgaacgaa ttgttaggtg
     4081 gcggtacttg ggtcgatatc aaagtgcatc acttcttccc gtatgcccaa ctttgtatag
     4141 agagccactg cgggatcgtc accgtaatct gcttgcacgt agatcacata agcaccaagc
     4201 gcgttggcct catgcttgag gagattgatg agcgcggtgg caatgccctg cctccggtgc
     4261 tcgccggaga ctgcgagatc atagatatag atctcactac gcggctgctc aaacttgggc
     4321 agaacgtaag ccgcgagagc gccaacaacc gcttcttggt cgaaggcagc aagcgcgatg
     4381 aatgtcttac tacggagcaa gttcccgagg taatcggagt ccggctgatg ttgggagtag
     4441 gtggctacgt ctccgaactc acgaccgaaa agatcaagag cagcccgcat ggatttgact
     4501 tggtcagggc cgagcctaca tgtgcgaatg atgcccatac ttgagccacc taactttgtt
     4561 ttagggcgac tgccctgctg cgtaacatcg ttgctgctgc gtaacatcgt tgctgctcca
     4621 taacatcaaa catcgaccca cggcgtaacg cgcttgctgc ttggatgccc gaggcataga
     4681 ctgtacaaaa aaacagtcat aacaagccat gaaaaccgcc actgcgccgt taccaccgct
     4741 gcgttcggtc aaggttctgg accagttgcg tgagcgcata cgctacttgc attacagttt
     4801 acgaaccgaa caggcttatg tcaactgggt ttgtgccttc atccgtttcc acggtgtgcg
     4861 tcacccggca accttgggca gcagcgaagt cgaggcattt ctgtcctggc tggcgaacga
     4921 gcgcaaggtt tcggtctcca cgcatcgtca ggcattggcg gccttgctgt tcttctacgg
     4981 caaggtgctg tgcacggatc tgcccttgct tcaggagatc ggtagacctc ggccgtcgcg
     5041 gcgcttgccg gtggtgctga ccccggatga agtggttcgc atcctcggtt ttctggaagg
     5101 cgagcatcgt ttgttcgccc aggactctag ctatagttct agtggttggc tacagctttg
     5161 tttgtactat caacaggttg aactgctgat caacagatcc tctacgcggc cgcggtacca
     5221 taacttcgta tagcatacat tatacgaagt tatctgggtt taaacgctct atggtctaaa
     5281 gtttttatcg aggctaactg aaacacggaa ggagacaata ccggaaggaa cccgcgctat
     5341 gacggcaata aaaagacaga ataaaacgca cgggtgttgg gtcgtttgtt cataaacgcg
     5401 gggttcggtc ccagggctgg cactctgtca agtgaacgct ctatggtcta aagatttaaa
     5461 tcgacctact ccggaatatt aatagatcat ggagataatt aaaatgataa ccatctcgca
     5521 aataaataag tattttactg ttttcgtaac agttttgtaa taaaaaaacc tataaatatt
     5581 ccggattatt cataccgtcc caccatcggg cgcggatcca tgtcgcgggg ctccatcgag
     5641 attcccctcc gggacactga cgaggtcatt gaacttgact tcgatcagtt accggaggga
     5701 gatgaagtta tcagtattct gaaacaggaa cacacacaac tgcacatatg gattgctttg
     5761 gcgctggaat actacaagca aggaaaaaca gaagagtttg taaaattgtt ggaagcagca
     5821 cgtatagatg gcaatttgga ctatagagac catgaaaaag accagatgac ttgcttggat
     5881 acattggcag cgtattatgt acaacaggct cggaaagaaa agaataagga caataaaaag
     5941 gatcttatta cacaggccac cttgttgtat acaatggccg ataaaattat tatgtatgat
     6001 cagaaccatt tgttgggaag agcctgcttc tgcctacttg agggtgacaa aatggatcaa
     6061 gctgatgcac agtttcattt tgtactcaat cagtctccaa ataatattcc agcccttctt
     6121 ggtaaagctt gcatttcctt caacaagaag gattacagag gagctcttgc ttactataag
     6181 aaagcattgc gtactaaccc aggatgtcca gcggaagttc gtttaggaat gggtcattgc
     6241 tttgtgaaac ttaacaaact ggaaaaagct cgtctggcat tcagcagagc cctggaactc
     6301 aattccaaat gcgtgggagc attggttgga ctggctgttc tagaactcaa caataaagag
     6361 gctgattcca ttaaaaatgg tgtccagctt ctttccagag cctatactat tgatcctagc
     6421 aaccctatgg tattgaacca tttggcaaat cactttttct tcaaaaagga ttatagtaaa
     6481 gtccagcatc tggccctcca tgcattccat aatacagaag tggaagctat gcaagcagag
     6541 agctgctatc agctagctag atcattccat gttcaggaag attatgacca agcttttcag
     6601 tactattatc aagccacaca gtttgcctca tcctcttttg tgctcccatt ttttggtttg
     6661 ggacaaatgt atatttatcg aggtgacaaa gaaaatgcat ctcagtgctt tgagaaggtt
     6721 ttgaaagctt atcctaataa ttacgaaact atgaaaattc tcggctctct ctatgctgcc
     6781 tcagaagatc aagaaaaacg agatattgcc aagggccatt tgaagaaggt cacagaacag
     6841 tatcccgatg atgttgaagc ttggattgaa ttggcacaaa tcttagaaca gactgatata
     6901 cagggtgccc tttcagccta tggaacagca acacgaatcc ttcaggagaa agtgcaggcc
     6961 gatgttcctc cagagattct caataatgtg ggtgccctcc attttagact tggaaaccta
     7021 ggggaggcta agaaatattt tttggcgtca ttggaccgtg caaaagcaga agcggaacac
     7081 gatgagcatt actataacgc catttccgtt actacgtcat ataatctcgc caggctatat
     7141 gaggcgatgt gtgaattcca tgaagcagaa aaactgtata aaaacatctt acgcgaacat
     7201 cctaattatg ttgactgcta tttgcgccta ggagccatgg ctagagataa gggaaacttt
     7261 tatgaggctt cagattggtt taaggaagct cttcagatta atcaggatca tccagatgct
     7321 tggtctttga ttggcaatct tcatttggca aaacaagaat ggggtcctgg gcagaagaag
     7381 tttgagagga tattaaaaca gccatccaca cagagtgata cctattctat gctagccctt
     7441 ggcaacgtgt ggctccaaac tttacatcag cccacccgag atcgagaaaa ggaaaagcgt
     7501 catcaagatc gtgctctggc catctacaaa caagtactca gaaatgatgc aaagaatctg
     7561 tatgctgcca atggcatagg agctgttttg gcccacaaag gatattttcg tgaagctcgt
     7621 gatgtatttg cccaagtaag agaagcaaca gcagatatta gtgatgtgtg gctgaactta
     7681 gcacacatct atgtggagca aaagcagtac atcagcgccg ttcagatgta tgaaaactgc
     7741 ctccgaaagt tctataagca ccaaaacact gaagttgtac tctatttggc ccgggccctc
     7801 ttcaagtgtg gcaagttaca ggaatgcaaa cagactttgc tgaaggctag acatgtggca
     7861 cccagtgata cagttcttat gtttaatgtg gccttggtcc tgcaaagatt agctacctct
     7921 gtcctgaaag atgaaaaaag taatctgaag gaagtactta atgctgtgaa agaactggag
     7981 cttgcacata gatacttcag ttatttgagt aaagtgggag ataaaatgag atttgatttg
     8041 gcccttgctg ctacagaagc caggcagtgt tctgacttac tgagccaggc ccagtaccat
     8101 gtggcccggg cacgcaaaca agatgaagaa gagcgggagc tgcgggccaa gcaagagcaa
     8161 gaaaaggagc tgttaaggca gaaacttctt aaagaacagg aagagaaacg tctcagagaa
     8221 aaggaagagc aaaagaaact tttggaacag cgggcccagt atgtggagaa gaccaaaaat
     8281 attcttatgt ttactggtga gactgaagca acaaaagaga agaaaagagg tggtggtggt
     8341 ggacggcgtt ctaagaaggg aggagagttt gatgaatttg tcaatgatga cactgatgat
     8401 gacctaccta tatccaaaaa gaagaagaga agaaagggta gtggcagtga acaagaaggt
     8461 gaagatgagg agggtggtga gagaaagaag aaaaagagga gaagacatcc aaagggagaa
     8521 gaaggatctg atgatgatga aacagaaaat ggccccaaac caaaaaaacg acgtccacca
     8581 aaagcagaga agaaaaaggc tcccaagcca gaacgtctgc ctccatcaat gaagggaaaa
     8641 ataaaatcca aagccataat ttcatcaagt gatgactctt cggatgagga taaacttaaa
     8701 attgctgatg aaggacatcc caggaacagc aacagcaaca gtgactcaga cgaggacgaa
     8761 caacgaaaga aatgtgcctc atcagagagt gattccgatg agaaccagaa caagtctggc
     8821 agcgaggccg gcagtccccg gaggccacga agacagcggt cagatcagga ctcagacagt
     8881 gaccagccat ccagaaagag aaggccctcc ggttctgagc agtctgacaa tgaatctgtg
     8941 cagtcaggga gaagccactc aggagtttct gagaacgact ctcgcccagc ttctccaagt
     9001 gccgaatcag atcacgaatc ggagagagga tctgataatg agggttctgg ccaaggctct
     9061 ggaaatgaat cggaaccaga gggatccaac aatgaggcct cagatagagg ctcagaacat
     9121 gggtcagatg atagtgacgg tgcacttgaa gtcctctttc agggaccctg gagccacccg
     9181 cagttcgaaa agtgaaagct tgtcgagaag tactagagga tcataatcag ccataccaca
     9241 tttgtagagg ttttacttgc tttaaaaaac ctcccacacc tccccctgaa cctgaaacat
     9301 aaaatgaatg caattgttgt tgttaacttg tttattgcag cttataatgg ttacaaataa
     9361 agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt
     9421 ttgtccaaac tcatcaatgt atcttatcat gtctggatct gatcactgct tgagcctaga
     9481 agatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca
     9541 ataactatca taaccattta aataaactgg atactattgc acgtttaaat cgacctactc
     9601 cggaatatta atagatcatg gagataatta aaatgataac catctcgcaa ataaataagt
     9661 attttactgt tttcgtaaca gttttgtaat aaaaaaacct ataaatattc cggattattc
     9721 ataccgtccc accatcgggc gcggatccat ggactacaag gacgacgatg acaagctcga
     9781 tcttgaagtc ctctttcagg gacccatggc ggacgtgctt agcgtcctgc gacagtacaa
     9841 catccagaag aaggagattg tggtgaaggg agacgaagtg atcttcgggg agttctcctg
     9901 gcccaagaat gtgaagacca actatgttgt ttgggggact ggaaaggaag gccaacccag
     9961 agagtactac acattggatt ccattttatt tctacttaat aacgtgcacc tttctcatcc
    10021 tgtttatgtc cgacgtgcag ctactgaaaa tattcctgtg gttagaagac ctgatcgaaa
    10081 agatctactt ggatatctca atggtgaagc gtcaacatcg gcaagtatag acagaagcgc
    10141 tcccttagaa ataggtcttc agcgatctac tcaagtcaaa cgagctgcag atgaagtttt
    10201 agcagaagca aagaaaccac gaattgagga tgaagagtgt gtgcgccttg ataaagagag
    10261 attggctgcc cgtttggagg gtcacaaaga agggattgta cagactgaac agattaggtc
    10321 tttgtctgaa gctatgtcag tggaaaaaat tgctgcaatc aaagccaaaa ttatggctaa
    10381 gaaaagatct actatcaaga ctgatctaga tgatgacata actgccctta aacagaggag
    10441 ttttgtggat gctgaggtag atgtgacccg agatattgtc agcagagaga gagtatggag
    10501 gacacgaaca actatcttac aaagcacagg aaagaatttt tccaagaaca tttttgcaat
    10561 tcttcaatct gtaaaagcca gagaagaagg gcgtgcacct gaacagcgac ctgccccaaa
    10621 tgcagcacct gtggatccca ctttgcgcac caaacagcct atcccagctg cctataacag
    10681 atacgatcag gaaagattca aaggaaaaga agaaacggaa ggcttcaaaa ttgacactat
    10741 gggaacctac catggtatga cactgaaatc tgtaacggag ggtgcatctg cccggaagac
    10801 tcagactcct gcagcccagc cagtaccaag accagtttct caagcaagac ctcccccaaa
    10861 tcagaagaaa ggatctcgaa cacccattat cataattcct gcagctacca cctctttaat
    10921 aaccatgctt aatgcaaaag accttctaca ggacctgaaa tttgtcccat cagatgaaaa
    10981 gaagaaacaa ggttgtcaac gagaaaatga aactctaata caaagaagaa aagaccagat
    11041 gcaaccaggg ggcactgcaa ttagtgttac agtaccttat agagtagtag accagcccct
    11101 taaacttatg cctcaagact gggaccgcgt tgtagccgtt tttgtgcagg gtcctgcatg
    11161 gcagttcaaa ggttggccat ggcttttgcc tgatggatca ccagttgata tatttgctaa
    11221 aattaaagcc ttccatctga agtatgatga agttcgtctg gatccaaatg ttcagaaatg
    11281 ggatgtaaca gtattagaac tcagctatca caaacgtcat ttggatagac cagtgttctt
    11341 acggttttgg gaaacattgg acaggtacat ggtaaagcat aaatcgcact tgagattctg
    11401 aaagcttgtc gagaagtact agaggatcat aatcagccat accacatttg tagaggtttt
    11461 acttgcttta aaaaacctcc cacacctccc cctgaacctg aaacataaaa tgaatgcaat
    11521 tgttgttgtt aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac
    11581 aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat
    11641 caatgtatct tatcatgtct ggatctgatc actgcttgag cctagaagat ccggctgcta
    11701 acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctatcataac
    11761 catttaaata aacctaatga tgcctgatgt ttaaatcgac ctactccgga atattaatag
    11821 atcatggaga taattaaaat gataaccatc tcgcaaataa ataagtattt tactgttttc
    11881 gtaacagttt tgtaataaaa aaacctataa atattccgga ttattcatac cgtcccacca
    11941 tcgggcgcgg atccatggcg gatatggagg atctcttcgg gagcgacgcc gacagcgaag
    12001 ctgagcgtaa agattctgat tctggatctg actcagattc tgatcaagag aatgctgcct
    12061 ctggcagtaa tgcctctgga agtgaaagtg atcaggatga aagaggtgat tcaggacaac
    12121 caagtaataa ggaactgttt ggagatgaca gtgaggacga gggagcttca catcatagtg
    12181 gtagtgataa tcactctgaa agatcagaca atagatcaga agcttctgag cgttctgacc
    12241 atgaggacaa tgacccctca gatgtagatc agcacagtgg atcagaagcc cctaatgatg
    12301 atgaagacga aggtcataga tcggatggag ggagccatca ttcagaagca gaaggttctg
    12361 aaaaagcaca ttcagatgat gaaaaatggg gcagagaaga taaaagtgac cagtcagatg
    12421 atgaaaagat acaaaattct gatgatgagg agagggcaca aggatctgat gaagataagc
    12481 tgcagaattc tgacgatgat gagaaaatgc agaacacaga tgatgaggag aggcctcagc
    12541 tttccgatga tgagagacaa cagctatctg aggaggaaaa ggctaattct gatgatgaac
    12601 ggccggtagc ttctgataat gatgatgaga aacagaattc tgatgatgaa gaacaaccac
    12661 agctgtctga tgaagagaaa atgcaaaatt ctgatgatga aaggccacag gcctcagatg
    12721 aagaacacag gcattcagat gatgaagagg aacaggatca taaatcagaa tctgcaagag
    12781 gcagtgatag tgaagatgaa gttttacgaa tgaaacgcaa gaatgcgatt gcatctgatt
    12841 cagaagcgga tagtgacact gaggtgccaa aagataatag tggaaccatg gatttatttg
    12901 gaggtgcaga tgatatctct tcagggagtg atggagaaga caaaccacct actccaggac
    12961 agcctgttga tgaaaatgga ttgcctcagg atcaacagga agaggagcca attcctgaga
    13021 ccagaataga agtagaaata cccaaagtaa acactgattt aggaaacgac ttatattttg
    13081 ttaaactgcc caactttctc agtgtagagc ccagaccttt tgatcctcag tattatgaag
    13141 atgaatttga agatgaagaa atgctggatg aagaaggtag aaccaggtta aaattaaagg
    13201 tagaaaatac tataagatgg aggatacgcc gagatgaaga aggaaatgaa attaaagaaa
    13261 gcaatgctcg gatagtcaag tggtcagatg gaagcatgtc cctgcattta ggcaatgaag
    13321 tgtttgatgt gtacaaagcc ccactgcagg gcgaccacaa tcatcttttt ataagacaag
    13381 gtactggtct acagggacaa gcagtcttta aaacgaaact caccttcaga cctcactcta
    13441 cggacagtgc cacacataga aagatgactc tgtcacttgc agataggtgt tcaaagacac
    13501 agaagattag aatcttgcca atggctggtc gtgatcctga atgccaacgc acagaaatga
    13561 ttaagaaaga agaagaacgt ttgagggctt ccatacgtag ggaatctcag cagcgccgaa
    13621 tgagagagaa acagcaccag cgggggctga gcgccagtta cctggaacct gatcgatacg
    13681 atgaggagga ggaaggcgag gagtccatca gcttggctgc cattaaaaac cgatataaag
    13741 ggggcattcg agaggaacga gccagaatct attcatcaga cagtgatgag ggatcagaag
    13801 aagataaagc tcaaagatta ctcaaagcaa agaaacttac cagtgatgag gaaggtgaac
    13861 cttccggaaa gagaaaagca gaagatgatg ataaagcaaa taaaaagcat aagaagtatg
    13921 tgatcagcga tgaagaggaa gaagatgatg attgaaagct tgtcgagaag tactagagga
    13981 tcataatcag ccataccaca tttgtagagg ttttacttgc tttaaaaaac ctcccacacc
    14041 tccccctgaa cctgaaacat aaaatgaatg caattgttgt tgttaacttg tttattgcag
    14101 cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt
    14161 cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggatct
    14221 gatcactgct tgagcctaga agatccggct gctaacaaag cccgaaagga agctgagttg
    14281 gctgctgcca ccgctgagca ataactatca taaccattta aataaacggt tcacatagct
    14341 tagtttaaat cgacctactc cggaatatta atagatcatg gagataatta aaatgataac
    14401 catctcgcaa ataaataagt attttactgt tttcgtaaca gttttgtaat aaaaaaacct
    14461 ataaatattc cggattattc ataccgtccc accatcgggc gcggatccat ggcgcccacc
    14521 atccagaccc aggcccagcg ggaggatggc cacaggccca attcccaccg gactctgcct
    14581 gagaggtctg gagtggtctg ccgagtcaag tactgcaata gcctccctga tatccccttc
    14641 gaccccaagt tcatcaccta ccccttcgac cagaacaggt tcgtccagta caaagccact
    14701 tccttggaga aacagcacaa acatgacctc ctgactgagc cagacctggg ggtcaccatc
    14761 gatctcatca atcctgacac ctaccgcatc gaccccaatg ttcttctaga tccagctgat
    14821 gagaaacttt tggaagagga gattcaggcc cccaccagct ccaagagatc ccagcagcac
    14881 gcgaaggtgg tgccatggat gcgaaagaca gagtacatct ccactgagtt caaccgttat
    14941 ggcatctcca atgagaagcc tgaggtcaag attggggttt ctgtgaagca gcagtttacc
    15001 gaggaagaaa tatacaaaga cagggatagc cagatcacag ccattgagaa gacttttgag
    15061 gatgcccaga aatcaatctc acagcattac agcaaacccc gagtcacacc ggtggaggtc
    15121 atgcctgtct tcccagactt taagatgtgg atcaatccat gtgctcaggt gatctttgac
    15181 tcagacccag cccccaagga cacgagtggt gcagctgcgt tggagatgat gtctcaggcc
    15241 atgattaggg gcatgatgga tgaggaaggg aaccagtttg tggcctattt cctgcctgta
    15301 gaagagacgt tgaagaaacg aaagcgggac caggaggagg agatggacta tgcaccagat
    15361 gatgtgtatg actacaaaat tgctcgggag tacaactgga acgtgaagaa caaagctagc
    15421 aagggctatg aggaaaacta cttcttcatc ttccgagagg gtgacggggt ttactacaat
    15481 gagttggaaa ccagggtccg ccttagtaag cgccgggcca aggctggggt tcagtcaggc
    15541 accaacgccc tgcttgtggt caaacatcgg gacatgaatg agaaggaact ggaagctcag
    15601 gaggcacgga aggcccagct agaaaaccac gaaccggagg aggaagagga agaggagatg
    15661 gagacagaag agaaagaagc tgggggctca gatgaggagc aggagaaggg cagcagcagt
    15721 gagaaggagg gcagtgaaga tgagcactcg ggcagcgaga gtgaacggga ggaaggtgac
    15781 agggacgagg ccagtgacaa gagtggcagt ggtgaggacg agagcagcga ggatgaggcc
    15841 cgggctgccc gtgacaaaga ggagatcttt ggcagtgatg ctgattctga ggacgatgcc
    15901 gactctgatg atgaggacag aggacaggcc caaggtggca gtgacaatga ttcagacagc
    15961 ggcagcaatg ggggtggcca gcggagccgg agccacagcc gcagcgccag tcccttcccc
    16021 agtggcagcg agcactcggc ccaggaggat ggcagtgaag ctgcagcttc tgattccagt
    16081 gaagctgata gtgacagtga ctgaaagctt gtcgagaagt actagaggat cataatcagc
    16141 cataccacat ttgtagaggt tttacttgct ttaaaaaacc tcccacacct ccccctgaac
    16201 ctgaaacata aaatgaatgc aattgttgtt gttaacttgt ttattgcagc ttataatggt
    16261 tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct
    16321 agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggatctg atcactgctt
    16381 gagcctagaa gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac
    16441 cgctgagcaa taactatcat aaccatttaa ataaacactg acattgactt ggtttaaatc
    16501 gacctactcc ggaatattaa tagatcatgg agataattaa aatgataacc atctcgcaaa
    16561 taaataagta ttttactgtt ttcgtaacag ttttgtaata aaaaaaccta taaatattcc
    16621 ggattattca taccgtccca ccatcgggcg cggatccatg accaaccagt acggtattct
    16681 cttcaaacaa gagcaagccc atgatgatgc catttggtca gttgcttggg ggacaaacaa
    16741 gaaggaaaac tctgagacag tggtcacagg ctccctagat gacctggtga aggtctggaa
    16801 atggcgtgat gagaggctgg acctacagtg gagtctggag ggacatcagc tgggagtggt
    16861 gtctgtggac atcagccaca ccctgcccat tgctgcatcc agctctcttg atgctcatat
    16921 tcgtctttgg gacttggaaa atggcaaaca gataaagtcc atagatgcag gacctgtgga
    16981 tgcctggact ttggcctttt ctcctgattc ccagtatctg gccacaggaa ctcatgtcgg
    17041 gaaagtgaac atttttggtg tggaaagtgg gaaaaaggaa tattctttgg acacgagagg
    17101 aaaattcatt cttagtattg catatagtcc tgatgggaaa tacctagcca gtggagccat
    17161 agatggaatc atcaatattt ttgatattgc aactggaaaa cttctgcata ccctggaagg
    17221 ccatgccatg cccattcgct ccttgacctt ttccccggac tcccagctcc ttgtcactgc
    17281 ttcagatgat ggctacatca agatctatga tgtacaacat gccaatttgg ctggcacgct
    17341 gagcggccat gcctcctggg tgctgaacgt tgcattctgt cctgatgaca ctcactttgt
    17401 ttccagttcg tctgacaaaa gtgtaaaagt ttgggatgtt ggaacgagga cttgtgttca
    17461 caccttcttt gatcaccagg atcaggtctg gggagtaaaa tacaatggaa atggttcaaa
    17521 aattgtgtct gttggagatg accaggaaat tcacatctat gattgtccaa tttaaaagct
    17581 tgtcgagaag tactagagga tcataatcag ccataccaca tttgtagagg ttttacttgc
    17641 tttaaaaaac ctcccacacc tccccctgaa cctgaaacat aaaatgaatg caattgttgt
    17701 tgttaacttg tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt
    17761 cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt
    17821 atcttatcat gtctggatct gatcactgct tgagcctaga agatccggct gctaacaaag
    17881 cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactatca taaccattta
    17941 aataaatcta tatctcaatc ggggttacgt cgatacccca ccgagacccc attgggacca
    18001 atacgcccgc gtttcttcct tttccccacc ccaaccccca agttcgggtg aaggcccagg
    18061 gctcgcagcc aacgtcgggg cggcaagccc tgccatagcc actacgggtt aattaaaaac
    18121 tatatctcaa tcggggttta aacaggagat ccgaaccaga taagtgaaat ctagttccaa
    18181 actattttgt catttttaat tttcgtatta gcttacgacg ctacacccag ttcccatcta
    18241 ttttgtcact cttccctaaa taatccttaa aaactccatt tccacccctc ccagttccca
    18301 actattttgt ccgcccacag cggggcattt ttcttcctgt tatgttttta atcaaacatc
    18361 ctgccaactc catgtgacaa accgtcatct tcggctactt t
//
