LOCUS       sequence-46362-e         784 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 784)
  AUTHORS   Scholl FA, Dumesic PA, Khavari PA
  TITLE     Mek1 alters epidermal growth and differentiation
  JOURNAL   Cancer Res. 2004 Sep 1;64(17):6035-40
  PUBMED    15342384
REFERENCE   2  (bases 1 to 784)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cancer 
            Res."; date: "2004-09-1"; volume: "64(17)"; pages: "6035-4"
COMMENT     Sequence Label: LZRS-Rfa
FEATURES             Location/Qualifiers
     source          1..784
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(join(89..95,96..113,114..119))
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     protein_bind    complement(144..268)
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     misc_feature    298..672
                     /label=pol region
                     /note="Moloney murine leukemia virus (MMLV) pol region 
                     containing the splice acceptor site"
     primer_bind     complement(344..360)
                     /label=pBMN 5'
                     /note="MMLV sequence, for inserts in pBMN retroviral 
                     vector"
     primer_bind     complement(698..714)
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     primer_bind     complement(730..752)
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
ORIGIN
        1 tcaacggtgg tatatccagt gatttttttc tccattttag cttccttagc tcctgaaaat
       61 ctcgacggat cctaactcaa aatccacaca ttatacgagc cggaagcata aagtgtaaag
      121 cctggggtgc ctaatgcggc cgccatagtg actggatatg ttgtgtttta cagtattatg
      181 tagtctgttt tttatgcaaa atctaattta atatattgat atttatatca ttttacgttt
      241 ctcgttcagc ttttttgtac aaacttgtga ttcccgtacc accacactgg gatccggcag
      301 tctagaggat ggtccacccc cggggtcggc agccttcacg tgggcggcgt gtatccaagc
      361 tgcgatgccg tctactttga gggcggtggg ggtggtcagc aggactgtgt aaggtccttt
      421 ccagcgaggt tctaggttct tagtctggtg tcggcggacc cacactgtgt cgccgactcg
      481 gtaagggtga ggtaccaccg gtcggtccag ttgttcttgg taggctgccg ccagaggtct
      541 ccagacttcg tgctggacta agtagagagc ctgtaagtga gcttggagag aggggctgtt
      601 agtaactctt gtcatgtcag ggtcagggaa gtttacaagg ggcgggggtg ccccatataa
      661 gatctcatat ggccatatgg gggcgcctag agaaggagtg agggctggat aaagggagga
      721 tcgaggcggg gtcgaacgag gaggttcaag gggggagaga cggggcggat ggaggaagag
      781 gagg
//
