LOCUS       sequence-463620-       15847 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Protein expression in insect cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15847)
  AUTHORS   Lopez Martinez D, Todorovski I, Noe Gonzalez M, Rusimbi C, Blears D,
            Khallou N, Han Z, Dirac-Svejstrup AB, Svejstrup JQ
  TITLE     PAF1C-mediated activation of CDK12/13 kinase activity is critical 
            for CTD phosphorylation and transcript elongation.
  JOURNAL   Mol Cell. 2025 May 15;85(10):1952-1967.e8. doi: 
            10.1016/j.molcel.2025.04.012. Epub 2025 May 1.
  PUBMED    40315851
REFERENCE   2  (bases 1 to 15847)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2025.04.012"; journalName: "Mol Cell"; date: 
            "2025-05-15- 15"; volume: "85"; issue: "10"; pages: "1952-1967.e8"
COMMENT     Sequence Label: pBIG1a-PAF1CdeltaLEO1
FEATURES             Location/Qualifiers
     source          1..15847
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(506..597)
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     polyA_signal    753..887
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(2613..2636)
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             complement(2643..2666)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     promoter        complement(2712..2803)
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     polyA_signal    2959..3093
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(3226..3249)
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             complement(3250..3273)
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     promoter        complement(6841..6932)
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     polyA_signal    7027..7075
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     primer_bind     7100..7119
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     protein_bind    7165..7198
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        join(7594..7599,7600..7616,7617..7622)
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     CDS             7811..8344
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     mobile_element  8411..8635
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     primer_bind     complement(8769..8786)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8940..9528)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9020..9039)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9699..10490,10491..10559))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10322..10341
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10560..10664)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(10690..11144)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(10827..10848)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     11038..11057
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     CDS             complement(11185..11976)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     mobile_element  12500..12665
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     polyA_signal    12980..13114
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        complement(14204..14295)
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     polyA_signal    14451..14585
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
ORIGIN
        1 tcttgacctc aggcttctca ttggagatgc cataacggtt gaactcagtg gagatgtact
       61 ctgtctttcg catccatggc accaccttcg cgtgctgctg ggatctcttg gagctggtgg
      121 gggcctgaat ctcctcttcc aaaagtttct catcagctgg atctagaaga acattggggt
      181 cgatgcggta ggtgtcagga ttgatgagat cgatggtgac ccccaggtct ggctcagtca
      241 ggaggtcatg tttgtgctgt ttctccaagg aagtggcttt gtactggacg aacctgttct
      301 ggtcgaaggg gtaggtgatg aacttggggt cgaaggggat atcagggagg ctattgcagt
      361 acttgactcg gcagaccact ccagacctct caggcagagt ccggtgggaa ttgggcctgt
      421 ggccatcctc ccgctgggcc tgggtctgga tggtgggcgc catggatccg cgcccgatgg
      481 tgggacggta tgaataatcc ggaatattta taggtttttt tattacaaaa ctgttacgaa
      541 aacagtaaaa tacttattta tttgcgagat ggttatcatt ttaattatct ccatgatcta
      601 ttaatattcc ggagtaggtc gatttaaaca tcaggcatca ttaggtttat ttaaatggtt
      661 atgatagtta ttgctcagcg gtggcagcag ccaactcagc ttcctttcgg gctttgttag
      721 cagccggatc ttctaggctc aagcagtgat cagatccaga catgataaga tacattgatg
      781 agtttggaca aaccacaact agaatgcagt gaaaaaaatg ctttatttgt gaaatttgtg
      841 atgctattgc tttatttgta accattataa gctgcaataa acaagttaac aacaacaatt
      901 gcattcattt tatgtttcag gttcaggggg aggtgtggga ggttttttaa agcaagtaaa
      961 acctctacaa atgtggtatg gctgattatg atcctctagt acttctcgac aagctttcag
     1021 aatctcaagt gcgatttatg ctttaccatg tacctgtcca atgtttccca aaaccgtaag
     1081 aacactggtc tatccaaatg acgtttgtga tagctgagtt ctaatactgt tacatcccat
     1141 ttctgaacat ttggatccag acgaacttca tcatacttca gatggaaggc tttaatttta
     1201 gcaaatatat caactggtga tccatcaggc aaaagccatg gccaaccttt gaactgccat
     1261 gcaggaccct gcacaaaaac ggctacaacg cggtcccagt cttgaggcat aagtttaagg
     1321 ggctggtcta ctactctata aggtactgta acactaattg cagtgccccc tggttgcatc
     1381 tggtcttttc ttctttgtat tagagtttca ttttctcgtt gacaaccttg tttcttcttt
     1441 tcatctgatg ggacaaattt caggtcctgt agaaggtctt ttgcattaag catggttatt
     1501 aaagaggtgg tagctgcagg aattatgata atgggtgttc gagatccttt cttctgattt
     1561 gggggaggtc ttgcttgaga aactggtctt ggtactggct gggctgcagg agtctgagtc
     1621 ttccgggcag atgcaccctc cgttacagat ttcagtgtca taccatggta ggttcccata
     1681 gtgtcaattt tgaagccttc cgtttcttct tttcctttga atctttcctg atcgtatctg
     1741 ttataggcag ctgggatagg ctgtttggtg cgcaaagtgg gatccacagg tgctgcattt
     1801 ggggcaggtc gctgttcagg tgcacgccct tcttctctgg cttttacaga ttgaagaatt
     1861 gcaaaaatgt tcttggaaaa attctttcct gtgctttgta agatagttgt tcgtgtcctc
     1921 catactctct ctctgctgac aatatctcgg gtcacatcta cctcagcatc cacaaaactc
     1981 ctctgtttaa gggcagttat gtcatcatct agatcagtct tgatagtaga tcttttctta
     2041 gccataattt tggctttgat tgcagcaatt ttttccactg acatagcttc agacaaagac
     2101 ctaatctgtt cagtctgtac aatcccttct ttgtgaccct ccaaacgggc agccaatctc
     2161 tctttatcaa ggcgcacaca ctcttcatcc tcaattcgtg gtttctttgc ttctgctaaa
     2221 acttcatctg cagctcgttt gacttgagta gatcgctgaa gacctatttc taagggagcg
     2281 cttctgtcta tacttgccga tgttgacgct tcaccattga gatatccaag tagatctttt
     2341 cgatcaggtc ttctaaccac aggaatattt tcagtagctg cacgtcggac ataaacagga
     2401 tgagaaaggt gcacgttatt aagtagaaat aaaatggaat ccaatgtgta gtactctctg
     2461 ggttggcctt cctttccagt cccccaaaca acatagttgg tcttcacatt cttgggccag
     2521 gagaactccc cgaagatcac ttcgtctccc ttcaccacaa tctccttctt ctggatgttg
     2581 tactgtcgca ggacgctaag cacgtccgcc atgggtccct gaaagaggac ttcaagatcg
     2641 agcttgtcat cgtcgtcctt gtagtccatg gatccgcgcc cgatggtggg acggtatgaa
     2701 taatccggaa tatttatagg tttttttatt acaaaactgt tacgaaaaca gtaaaatact
     2761 tatttatttg cgagatggtt atcattttaa ttatctccat gatctattaa tattccggag
     2821 taggtcgatt taaacgtgca atagtatcca gtttatttaa atggttatga tagttattgc
     2881 tcagcggtgg cagcagccaa ctcagcttcc tttcgggctt tgttagcagc cggatcttct
     2941 aggctcaagc agtgatcaga tccagacatg ataagataca ttgatgagtt tggacaaacc
     3001 acaactagaa tgcagtgaaa aaaatgcttt atttgtgaaa tttgtgatgc tattgcttta
     3061 tttgtaacca ttataagctg caataaacaa gttaacaaca acaattgcat tcattttatg
     3121 tttcaggttc agggggaggt gtgggaggtt ttttaaagca agtaaaacct ctacaaatgt
     3181 ggtatggctg attatgatcc tctagtactt ctcgacaagc tttcactttt cgaactgcgg
     3241 gtggctccag ggtccctgaa agaggacttc aagtgcaccg tcactatcat ctgacccatg
     3301 ttctgagcct ctatctgagg cctcattgtt ggatccctct ggttccgatt catttccaga
     3361 gccttggcca gaaccctcat tatcagatcc tctctccgat tcgtgatctg attcggcact
     3421 tggagaagct gggcgagagt cgttctcaga aactcctgag tggcttctcc ctgactgcac
     3481 agattcattg tcagactgct cagaaccgga gggccttctc tttctggatg gctggtcact
     3541 gtctgagtcc tgatctgacc gctgtcttcg tggcctccgg ggactgccgg cctcgctgcc
     3601 agacttgttc tggttctcat cggaatcact ctctgatgag gcacatttct ttcgttgttc
     3661 gtcctcgtct gagtcactgt tgctgttgct gttcctggga tgtccttcat cagcaatttt
     3721 aagtttatcc tcatccgaag agtcatcact tgatgaaatt atggctttgg attttatttt
     3781 tcccttcatt gatggaggca gacgttctgg cttgggagcc tttttcttct ctgcttttgg
     3841 tggacgtcgt ttttttggtt tggggccatt ttctgtttca tcatcatcag atccttcttc
     3901 tccctttgga tgtcttctcc tctttttctt ctttctctca ccaccctcct catcttcacc
     3961 ttcttgttca ctgccactac cctttcttct cttcttcttt ttggatatag gtaggtcatc
     4021 atcagtgtca tcattgacaa attcatcaaa ctctcctccc ttcttagaac gccgtccacc
     4081 accaccacct cttttcttct cttttgttgc ttcagtctca ccagtaaaca taagaatatt
     4141 tttggtcttc tccacatact gggcccgctg ttccaaaagt ttcttttgct cttccttttc
     4201 tctgagacgt ttctcttcct gttctttaag aagtttctgc cttaacagct ccttttcttg
     4261 ctcttgcttg gcccgcagct cccgctcttc ttcatcttgt ttgcgtgccc gggccacatg
     4321 gtactgggcc tggctcagta agtcagaaca ctgcctggct tctgtagcag caagggccaa
     4381 atcaaatctc attttatctc ccactttact caaataactg aagtatctat gtgcaagctc
     4441 cagttctttc acagcattaa gtacttcctt cagattactt ttttcatctt tcaggacaga
     4501 ggtagctaat ctttgcagga ccaaggccac attaaacata agaactgtat cactgggtgc
     4561 cacatgtcta gccttcagca aagtctgttt gcattcctgt aacttgccac acttgaagag
     4621 ggcccgggcc aaatagagta caacttcagt gttttggtgc ttatagaact ttcggaggca
     4681 gttttcatac atctgaacgg cgctgatgta ctgcttttgc tccacataga tgtgtgctaa
     4741 gttcagccac acatcactaa tatctgctgt tgcttctctt acttgggcaa atacatcacg
     4801 agcttcacga aaatatcctt tgtgggccaa aacagctcct atgccattgg cagcatacag
     4861 attctttgca tcatttctga gtacttgttt gtagatggcc agagcacgat cttgatgacg
     4921 cttttccttt tctcgatctc gggtgggctg atgtaaagtt tggagccaca cgttgccaag
     4981 ggctagcata gaataggtat cactctgtgt ggatggctgt tttaatatcc tctcaaactt
     5041 cttctgccca ggaccccatt cttgttttgc caaatgaaga ttgccaatca aagaccaagc
     5101 atctggatga tcctgattaa tctgaagagc ttccttaaac caatctgaag cctcataaaa
     5161 gtttccctta tctctagcca tggctcctag gcgcaaatag cagtcaacat aattaggatg
     5221 ttcgcgtaag atgtttttat acagtttttc tgcttcatgg aattcacaca tcgcctcata
     5281 tagcctggcg agattatatg acgtagtaac ggaaatggcg ttatagtaat gctcatcgtg
     5341 ttccgcttct gcttttgcac ggtccaatga cgccaaaaaa tatttcttag cctcccctag
     5401 gtttccaagt ctaaaatgga gggcacccac attattgaga atctctggag gaacatcggc
     5461 ctgcactttc tcctgaagga ttcgtgttgc tgttccatag gctgaaaggg caccctgtat
     5521 atcagtctgt tctaagattt gtgccaattc aatccaagct tcaacatcat cgggatactg
     5581 ttctgtgacc ttcttcaaat ggcccttggc aatatctcgt ttttcttgat cttctgaggc
     5641 agcatagaga gagccgagaa ttttcatagt ttcgtaatta ttaggataag ctttcaaaac
     5701 cttctcaaag cactgagatg cattttcttt gtcacctcga taaatataca tttgtcccaa
     5761 accaaaaaat gggagcacaa aagaggatga ggcaaactgt gtggcttgat aatagtactg
     5821 aaaagcttgg tcataatctt cctgaacatg gaatgatcta gctagctgat agcagctctc
     5881 tgcttgcata gcttccactt ctgtattatg gaatgcatgg agggccagat gctggacttt
     5941 actataatcc tttttgaaga aaaagtgatt tgccaaatgg ttcaatacca tagggttgct
     6001 aggatcaata gtataggctc tggaaagaag ctggacacca tttttaatgg aatcagcctc
     6061 tttattgttg agttctagaa cagccagtcc aaccaatgct cccacgcatt tggaattgag
     6121 ttccagggct ctgctgaatg ccagacgagc tttttccagt ttgttaagtt tcacaaagca
     6181 atgacccatt cctaaacgaa cttccgctgg acatcctggg ttagtacgca atgctttctt
     6241 atagtaagca agagctcctc tgtaatcctt cttgttgaag gaaatgcaag ctttaccaag
     6301 aagggctgga atattatttg gagactgatt gagtacaaaa tgaaactgtg catcagcttg
     6361 atccattttg tcaccctcaa gtaggcagaa gcaggctctt cccaacaaat ggttctgatc
     6421 atacataata attttatcgg ccattgtata caacaaggtg gcctgtgtaa taagatcctt
     6481 tttattgtcc ttattctttt ctttccgagc ctgttgtaca taatacgctg ccaatgtatc
     6541 caagcaagtc atctggtctt tttcatggtc tctatagtcc aaattgccat ctatacgtgc
     6601 tgcttccaac aattttacaa actcttctgt ttttccttgc ttgtagtatt ccagcgccaa
     6661 agcaatccat atgtgcagtt gtgtgtgttc ctgtttcaga atactgataa cttcatctcc
     6721 ctccggtaac tgatcgaagt caagttcaat gacctcgtca gtgtcccgga ggggaatctc
     6781 gatggagccc cgcgacatgg atccgcgccc gatggtggga cggtatgaat aatccggaat
     6841 atttataggt ttttttatta caaaactgtt acgaaaacag taaaatactt atttatttgc
     6901 gagatggtta tcattttaat tatctccatg atctattaat attccggagt aggtcgattt
     6961 aaatctttag accatagagc gttcacttga cagagtgcca gccctgggac cgaaccccgc
     7021 gtttatgaac aaacgaccca acacccgtgc gttttattct gtctttttat tgccgtcata
     7081 gcgcgggttc cttccggtat tgtctccttc cgtgtttcag ttagcctcga taaaaacttt
     7141 agaccataga gcgtttaaac ccagataact tcgtataatg tatgctatac gaagttatgg
     7201 taccgcggcc gcgtagagga tctgttgatc agcagttcaa cctgttgata gtacaaacaa
     7261 agctgtagcc aaccactaga actatagcta gagtcctggg cgaacaaacg atgctcgcct
     7321 tccagaaaac cgaggatgcg aaccacttca tccggggtca gcaccaccgg caagcgccgc
     7381 gacggccgag gtctaccgat ctcctgaagc aagggcagat ccgtgcacag caccttgccg
     7441 tagaagaaca gcaaggccgc caatgcctga cgatgcgtgg agaccgaaac cttgcgctcg
     7501 ttcgccagcc aggacagaaa tgcctcgact tcgctgctgc ccaaggttgc cgggtgacgc
     7561 acaccgtgga aacggatgaa ggcacaaacc cagttgacat aagcctgttc ggttcgtaaa
     7621 ctgtaatgca agtagcgtat gcgctcacgc aactggtcca gaaccttgac cgaacgcagc
     7681 ggtggtaacg gcgcagtggc ggttttcatg gcttgttatg actgtttttt tgtacagtct
     7741 atgcctcggg catccaagca gcaagcgcgt tacgccgtgg gtcgatgttt gatgttatgg
     7801 agcagcaacg atgttacgca gcagcaacga tgttacgcag cagggcagtc gccctaaaac
     7861 aaagttaggt ggctcaagta tgggcatcat tcgcacatgt aggctcggcc ctgaccaagt
     7921 caaatccatg cgggctgctc ttgatctttt cggtcgtgag ttcggagacg tagccaccta
     7981 ctcccaacat cagccggact ccgattacct cgggaacttg ctccgtagta agacattcat
     8041 cgcgcttgct gccttcgacc aagaagcggt tgttggcgct ctcgcggctt acgttctgcc
     8101 caagtttgag cagccgcgta gtgagatcta tatctatgat ctcgcagtct ccggcgagca
     8161 ccggaggcag ggcattgcca ccgcgctcat caatctcctc aagcatgagg ccaacgcgct
     8221 tggtgcttat gtgatctacg tgcaagcaga ttacggtgac gatcccgcag tggctctcta
     8281 tacaaagttg ggcatacggg aagaagtgat gcactttgat atcgacccaa gtaccgccac
     8341 ctaacaattc gttcaagccg agatcggctt cccggccgcg gagttgttcg gtaaattgtc
     8401 acaacgccgc gaatatagtc tttaccatgc ccttggccac gcccctcttt aatacgacgg
     8461 gcaatttgca cttcagaaaa tgaagagttt gctttagcca taacaaaagt ccagtatgct
     8521 ttttcacagc ataactggac tgatttcagt ttacaactat tctgtctagt ttaagacttt
     8581 attgtcatag tttagatcta ttttgttcag tttaagactt tattgtccgc ccacacccgc
     8641 ttacgcaggg catccattta ttactcaacc gtaaccgatt ttgccaggtt acgcggctgg
     8701 tctatgcggt gtgaaatacc gcacagatgc gtaaggagaa aataccgcat caggcgctct
     8761 tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca
     8821 gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac
     8881 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt
     8941 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg
     9001 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc
     9061 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc
     9121 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc
     9181 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac
     9241 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt
     9301 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct
     9361 aactacggct acactagaag aatagtattt ggtatctgcg ctctgctgaa gccagttacc
     9421 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt
     9481 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg
     9541 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc
     9601 atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa
     9661 tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag
     9721 gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg
     9781 tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga
     9841 gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag
     9901 cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa
     9961 gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc
    10021 atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca
    10081 aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg
    10141 atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat
    10201 aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc
    10261 aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg
    10321 gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg
    10381 gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt
    10441 gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca
    10501 ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata
    10561 ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac
    10621 atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa
    10681 gtgccaccta aattgtaagc gttaatattt tgttaaaatt cgcgttaaat ttttgttaaa
    10741 tcagctcatt ttttaaccaa taggccgaaa tcggcaaaat cccttataaa tcaaaagaat
    10801 agaccgagat agggttgagt gttgttccag tttggaacaa gagtccacta ttaagaacgt
    10861 ggactccaac gtcaaagggc gaaaaaccgt ctatcagggc gatggcccac tacgtgaacc
    10921 atcaccctaa tcaagttttt tggggtcgag gtgccgtaaa gcactaaatc ggaaccctaa
    10981 agggagcccc cgatttagag cttgacgggg aaagccggcg aacgtggcga gaaaggaagg
    11041 gaagaaagcg aaaggagcgg gcgctagggc gctggcaagt gtagcggtca cgctgcgcgt
    11101 aaccaccaca cccgccgcgc ttaatgcgcc gctacagggc gcgtcccatt cgccaatccg
    11161 tcggcttgaa cgaattgtta gacattattt gccgactacc ttggtgatct cgcctttcac
    11221 gtagtggaca aattcttcca actgatctgc gcgcgaggcc aagcgatctt cttcttgtcc
    11281 aagataagcc tgtctagctt caagtatgac gggctgatac tgggccggca ggcgctccat
    11341 tgcccagtcg gcagcgacat ccttcggcgc gattttgccg gttactgcgc tgtaccaaat
    11401 gcgggacaac gtaagcacta catttcgctc atcgccagcc cagtcgggcg gcgagttcca
    11461 tagcgttaag gtttcattta gcgcctcaaa tagatcctgt tcaggaaccg gatcaaagag
    11521 ttcctccgcc gctggaccta ccaaggcaac gctatgttct cttgcttttg tcagcaagat
    11581 agccagatca atgtcgatcg tggctggctc gaagatacct gcaagaatgt cattgcgctg
    11641 ccattctcca aattgcagtt cgcgcttagc tggataacgc cacggaatga tgtcgtcgtg
    11701 cacaacaatg gtgacttcta cagcgcggag aatctcgctc tctccagggg aagccgaagt
    11761 ttccaaaagg tcgttgatca aagctcgccg cgttgtttca tcaagcctta cggtcaccgt
    11821 aaccagcaaa tcaatatcac tgtgtggctt caggccgcca tccactgcgg agccgtacaa
    11881 atgtacggcc agcaacgtcg gttcgagatg gcgctcgatg acgccaacta cctctgatag
    11941 ttgagtcgat acttcggcga tcaccgcttc cctcatgatg tttaactttg ttttagggcg
    12001 actgccctgc tgcgtaacat cgttgctgct ccataacatc aaacatcgac ccacggcgta
    12061 acgcgcttgc tgcttggatg cccgaggcat agactgtacc ccaaaaaaac agtcataaca
    12121 agccatgaaa accgccactg cgccgttacc accgctgcgt tcggtcaagg ttctggacca
    12181 gttgcgtgag cgcatacgct acttaccggt ttattgacta ccggaagcag tgtgaccgtg
    12241 tgcttctcaa atgcctgagg ccagtttgct caggctctcc ccgtggaggt aataattgac
    12301 gatatgatca tttattctgc ctcccagttc aggctgcaaa taagcgttga tattcagtca
    12361 attacaaaca ttaataacga agagatgaca gaaaaatttt cattctgtga cagagaaaaa
    12421 gtagccgaag atgacggttt gtcacatgga gttggcagga tgtttgatta aaaacataac
    12481 aggaagaaaa atgccccgct gtgggcggac aaaatagttg ggaactggga ggggtggaaa
    12541 tggagttttt aaggattatt tagggaagag tgacaaaata gatgggaact gggtgtagcg
    12601 tcgtaagcta atacgaaaat taaaaatgac aaaatagttt ggaactagat ttcacttatc
    12661 tggttcggat ctcctgttta aaccccgatt gagatatagt ttttaattaa cccgtagtgg
    12721 ctatggcagg gcttgccgcc ccgacgttgg ctgcgagccc tgggccttca cccgaacttg
    12781 ggggttgggg tggggaaaag gaagaaacgc gggcgtattg gtcccaatgg ggtctcggtg
    12841 gggtatcgac gtaaccccga ttgagatata gatttattta aatggttatg atagttattg
    12901 ctcagcggtg gcagcagcca actcagcttc ctttcgggct ttgttagcag ccggatcttc
    12961 taggctcaag cagtgatcag atccagacat gataagatac attgatgagt ttggacaaac
    13021 cacaactaga atgcagtgaa aaaaatgctt tatttgtgaa atttgtgatg ctattgcttt
    13081 atttgtaacc attataagct gcaataaaca agttaacaac aacaattgca ttcattttat
    13141 gtttcaggtt cagggggagg tgtgggaggt tttttaaagc aagtaaaacc tctacaaatg
    13201 tggtatggct gattatgatc ctctagtact tctcgacaag cttttaaatt ggacaatcat
    13261 agatgtgaat ttcctggtca tctccaacag acacaatttt tgaaccattt ccattgtatt
    13321 ttactcccca gacctgatcc tggtgatcaa agaaggtgtg aacacaagtc ctcgttccaa
    13381 catcccaaac ttttacactt ttgtcagacg aactggaaac aaagtgagtg tcatcaggac
    13441 agaatgcaac gttcagcacc caggaggcat ggccgctcag cgtgccagcc aaattggcat
    13501 gttgtacatc atagatcttg atgtagccat catctgaagc agtgacaagg agctgggagt
    13561 ccggggaaaa ggtcaaggag cgaatgggca tggcatggcc ttccagggta tgcagaagtt
    13621 ttccagttgc aatatcaaaa atattgatga ttccatctat ggctccactg gctaggtatt
    13681 tcccatcagg actatatgca atactaagaa tgaattttcc tctcgtgtcc aaagaatatt
    13741 cctttttccc actttccaca ccaaaaatgt tcactttccc gacatgagtt cctgtggcca
    13801 gatactggga atcaggagaa aaggccaaag tccaggcatc cacaggtcct gcatctatgg
    13861 actttatctg tttgccattt tccaagtccc aaagacgaat atgagcatca agagagctgg
    13921 atgcagcaat gggcagggtg tggctgatgt ccacagacac cactcccagc tgatgtccct
    13981 ccagactcca ctgtaggtcc agcctctcat cacgccattt ccagaccttc accaggtcat
    14041 ctagggagcc tgtgaccact gtctcagagt tttccttctt gtttgtcccc caagcaactg
    14101 accaaatggc atcatcatgg gcttgctctt gtttgaagag aataccgtac tggttggtca
    14161 tggatccgcg cccgatggtg ggacggtatg aataatccgg aatatttata ggttttttta
    14221 ttacaaaact gttacgaaaa cagtaaaata cttatttatt tgcgagatgg ttatcatttt
    14281 aattatctcc atgatctatt aatattccgg agtaggtcga tttaaactaa gctatgtgaa
    14341 ccgtttattt aaatggttat gatagttatt gctcagcggt ggcagcagcc aactcagctt
    14401 cctttcgggc tttgttagca gccggatctt ctaggctcaa gcagtgatca gatccagaca
    14461 tgataagata cattgatgag tttggacaaa ccacaactag aatgcagtga aaaaaatgct
    14521 ttatttgtga aatttgtgat gctattgctt tatttgtaac cattataagc tgcaataaac
    14581 aagttaacaa caacaattgc attcatttta tgtttcaggt tcagggggag gtgtgggagg
    14641 ttttttaaag caagtaaaac ctctacaaat gtggtatggc tgattatgat cctctagtac
    14701 ttctcgacaa gctttcagtc actgtcacta tcagcttcac tggaatcaga agctgcagct
    14761 tcactgccat cctcctgggc cgagtgctcg ctgccactgg ggaagggact ggcgctgcgg
    14821 ctgtggctcc ggctccgctg gccaccccca ttgctgccgc tgtctgaatc attgtcactg
    14881 ccaccttggg cctgtcctct gtcctcatca tcagagtcgg catcgtcctc agaatcagca
    14941 tcactgccaa agatctcctc tttgtcacgg gcagcccggg cctcatcctc gctgctctcg
    15001 tcctcaccac tgccactctt gtcactggcc tcgtccctgt caccttcctc ccgttcactc
    15061 tcgctgcccg agtgctcatc ttcactgccc tccttctcac tgctgctgcc cttctcctgc
    15121 tcctcatctg agcccccagc ttctttctct tctgtctcca tctcctcttc ctcttcctcc
    15181 tccggttcgt ggttttctag ctgggccttc cgtgcctcct gagcttccag ttccttctca
    15241 ttcatgtccc gatgtttgac cacaagcagg gcgttggtgc ctgactgaac cccagccttg
    15301 gcccggcgct tactaaggcg gaccctggtt tccaactcat tgtagtaaac cccgtcaccc
    15361 tctcggaaga tgaagaagta gttttcctca tagcccttgc tagctttgtt cttcacgttc
    15421 cagttgtact cccgagcaat tttgtagtca tacacatcat ctggtgcata gtccatctcc
    15481 tcctcctggt cccgctttcg tttcttcaac gtctcttcta caggcaggaa ataggccaca
    15541 aactggttcc cttcctcatc catcatgccc ctaatcatgg cctgagacat catctccaac
    15601 gcagctgcac cactcgtgtc cttgggggct gggtctgagt caaagatcac ctgagcacat
    15661 ggattgatcc acatcttaaa gtctgggaag acaggcatga cctccaccgg tgtgactcgg
    15721 ggtttgctgt aatgctgtga gattgatttc tgggcatcct caaaagtctt ctcaatggct
    15781 gtgatctggc tatccctgtc tttgtatatt tcttcctcgg taaactgctg cttcacagaa
    15841 accccaa
//
