LOCUS       sequence-463628-       18296 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Protein expression in insect cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 18296)
  AUTHORS   Lopez Martinez D, Todorovski I, Noe Gonzalez M, Rusimbi C, Blears D,
            Khallou N, Han Z, Dirac-Svejstrup AB, Svejstrup JQ
  TITLE     PAF1C-mediated activation of CDK12/13 kinase activity is critical 
            for CTD phosphorylation and transcript elongation.
  JOURNAL   Mol Cell. 2025 May 15;85(10):1952-1967.e8. doi: 
            10.1016/j.molcel.2025.04.012. Epub 2025 May 1.
  PUBMED    40315851
REFERENCE   2  (bases 1 to 18296)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2025.04.012"; journalName: "Mol Cell"; date: 
            "2025-05-15- 15"; volume: "85"; issue: "10"; pages: "1952-1967.e8"
COMMENT     Sequence Label: pBIG1a-PAF1C-CDC73deltaKIM
FEATURES             Location/Qualifiers
     source          1..18296
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(25..613)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(105..124)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(784..1575,1576..1644))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1407..1426
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1645..1749)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(1775..2229)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1912..1933)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     2123..2142
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     CDS             complement(2270..3061)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     mobile_element  3585..3750
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     polyA_signal    4065..4199
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        complement(5289..5380)
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     polyA_signal    5536..5670
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        complement(7438..7529)
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     polyA_signal    7685..7819
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        complement(9992..10083)
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     polyA_signal    10239..10373
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(11994..12017)
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             complement(12024..12047)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     promoter        complement(12093..12184)
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     polyA_signal    12340..12474
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(12607..12630)
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             complement(12631..12654)
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     promoter        complement(16222..16313)
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     polyA_signal    16408..16456
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     primer_bind     16481..16500
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     protein_bind    16546..16579
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        join(16975..16980,16981..16997,16998..17003)
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     CDS             17192..17725
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     mobile_element  17792..18016
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     primer_bind     complement(18150..18167)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa
       61 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt
      121 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct
      181 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct
      241 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc
      301 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt
      361 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc
      421 tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaatag tatttggtat
      481 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa
      541 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa
      601 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga
      661 aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct
      721 tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga
      781 cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc
      841 catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg
      901 ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat
      961 aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat
     1021 ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg
     1081 caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc
     1141 attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa
     1201 agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc
     1261 actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt
     1321 ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag
     1381 ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt
     1441 gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag
     1501 atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac
     1561 cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc
     1621 gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca
     1681 gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg
     1741 ggttccgcgc acatttcccc gaaaagtgcc acctaaattg taagcgttaa tattttgtta
     1801 aaattcgcgt taaatttttg ttaaatcagc tcatttttta accaataggc cgaaatcggc
     1861 aaaatccctt ataaatcaaa agaatagacc gagatagggt tgagtgttgt tccagtttgg
     1921 aacaagagtc cactattaag aacgtggact ccaacgtcaa agggcgaaaa accgtctatc
     1981 agggcgatgg cccactacgt gaaccatcac cctaatcaag ttttttgggg tcgaggtgcc
     2041 gtaaagcact aaatcggaac cctaaaggga gcccccgatt tagagcttga cggggaaagc
     2101 cggcgaacgt ggcgagaaag gaagggaaga aagcgaaagg agcgggcgct agggcgctgg
     2161 caagtgtagc ggtcacgctg cgcgtaacca ccacacccgc cgcgcttaat gcgccgctac
     2221 agggcgcgtc ccattcgcca atccgtcggc ttgaacgaat tgttagacat tatttgccga
     2281 ctaccttggt gatctcgcct ttcacgtagt ggacaaattc ttccaactga tctgcgcgcg
     2341 aggccaagcg atcttcttct tgtccaagat aagcctgtct agcttcaagt atgacgggct
     2401 gatactgggc cggcaggcgc tccattgccc agtcggcagc gacatccttc ggcgcgattt
     2461 tgccggttac tgcgctgtac caaatgcggg acaacgtaag cactacattt cgctcatcgc
     2521 cagcccagtc gggcggcgag ttccatagcg ttaaggtttc atttagcgcc tcaaatagat
     2581 cctgttcagg aaccggatca aagagttcct ccgccgctgg acctaccaag gcaacgctat
     2641 gttctcttgc ttttgtcagc aagatagcca gatcaatgtc gatcgtggct ggctcgaaga
     2701 tacctgcaag aatgtcattg cgctgccatt ctccaaattg cagttcgcgc ttagctggat
     2761 aacgccacgg aatgatgtcg tcgtgcacaa caatggtgac ttctacagcg cggagaatct
     2821 cgctctctcc aggggaagcc gaagtttcca aaaggtcgtt gatcaaagct cgccgcgttg
     2881 tttcatcaag ccttacggtc accgtaacca gcaaatcaat atcactgtgt ggcttcaggc
     2941 cgccatccac tgcggagccg tacaaatgta cggccagcaa cgtcggttcg agatggcgct
     3001 cgatgacgcc aactacctct gatagttgag tcgatacttc ggcgatcacc gcttccctca
     3061 tgatgtttaa ctttgtttta gggcgactgc cctgctgcgt aacatcgttg ctgctccata
     3121 acatcaaaca tcgacccacg gcgtaacgcg cttgctgctt ggatgcccga ggcatagact
     3181 gtaccccaaa aaaacagtca taacaagcca tgaaaaccgc cactgcgccg ttaccaccgc
     3241 tgcgttcggt caaggttctg gaccagttgc gtgagcgcat acgctactta ccggtttatt
     3301 gactaccgga agcagtgtga ccgtgtgctt ctcaaatgcc tgaggccagt ttgctcaggc
     3361 tctccccgtg gaggtaataa ttgacgatat gatcatttat tctgcctccc agttcaggct
     3421 gcaaataagc gttgatattc agtcaattac aaacattaat aacgaagaga tgacagaaaa
     3481 attttcattc tgtgacagag aaaaagtagc cgaagatgac ggtttgtcac atggagttgg
     3541 caggatgttt gattaaaaac ataacaggaa gaaaaatgcc ccgctgtggg cggacaaaat
     3601 agttgggaac tgggaggggt ggaaatggag tttttaagga ttatttaggg aagagtgaca
     3661 aaatagatgg gaactgggtg tagcgtcgta agctaatacg aaaattaaaa atgacaaaat
     3721 agtttggaac tagatttcac ttatctggtt cggatctcct gtttaaaccc cgattgagat
     3781 atagttttta attaacccgt agtggctatg gcagggcttg ccgccccgac gttggctgcg
     3841 agccctgggc cttcacccga acttgggggt tggggtgggg aaaaggaaga aacgcgggcg
     3901 tattggtccc aatggggtct cggtggggta tcgacgtaac cccgattgag atatagattt
     3961 atttaaatgg ttatgatagt tattgctcag cggtggcagc agccaactca gcttcctttc
     4021 gggctttgtt agcagccgga tcttctaggc tcaagcagtg atcagatcca gacatgataa
     4081 gatacattga tgagtttgga caaaccacaa ctagaatgca gtgaaaaaaa tgctttattt
     4141 gtgaaatttg tgatgctatt gctttatttg taaccattat aagctgcaat aaacaagtta
     4201 acaacaacaa ttgcattcat tttatgtttc aggttcaggg ggaggtgtgg gaggtttttt
     4261 aaagcaagta aaacctctac aaatgtggta tggctgatta tgatcctcta gtacttctcg
     4321 acaagctttt aaattggaca atcatagatg tgaatttcct ggtcatctcc aacagacaca
     4381 atttttgaac catttccatt gtattttact ccccagacct gatcctggtg atcaaagaag
     4441 gtgtgaacac aagtcctcgt tccaacatcc caaactttta cacttttgtc agacgaactg
     4501 gaaacaaagt gagtgtcatc aggacagaat gcaacgttca gcacccagga ggcatggccg
     4561 ctcagcgtgc cagccaaatt ggcatgttgt acatcataga tcttgatgta gccatcatct
     4621 gaagcagtga caaggagctg ggagtccggg gaaaaggtca aggagcgaat gggcatggca
     4681 tggccttcca gggtatgcag aagttttcca gttgcaatat caaaaatatt gatgattcca
     4741 tctatggctc cactggctag gtatttccca tcaggactat atgcaatact aagaatgaat
     4801 tttcctctcg tgtccaaaga atattccttt ttcccacttt ccacaccaaa aatgttcact
     4861 ttcccgacat gagttcctgt ggccagatac tgggaatcag gagaaaaggc caaagtccag
     4921 gcatccacag gtcctgcatc tatggacttt atctgtttgc cattttccaa gtcccaaaga
     4981 cgaatatgag catcaagaga gctggatgca gcaatgggca gggtgtggct gatgtccaca
     5041 gacaccactc ccagctgatg tccctccaga ctccactgta ggtccagcct ctcatcacgc
     5101 catttccaga ccttcaccag gtcatctagg gagcctgtga ccactgtctc agagttttcc
     5161 ttcttgtttg tcccccaagc aactgaccaa atggcatcat catgggcttg ctcttgtttg
     5221 aagagaatac cgtactggtt ggtcatggat ccgcgcccga tggtgggacg gtatgaataa
     5281 tccggaatat ttataggttt ttttattaca aaactgttac gaaaacagta aaatacttat
     5341 ttatttgcga gatggttatc attttaatta tctccatgat ctattaatat tccggagtag
     5401 gtcgatttaa accaagtcaa tgtcagtgtt tatttaaatg gttatgatag ttattgctca
     5461 gcggtggcag cagccaactc agcttccttt cgggctttgt tagcagccgg atcttctagg
     5521 ctcaagcagt gatcagatcc agacatgata agatacattg atgagtttgg acaaaccaca
     5581 actagaatgc agtgaaaaaa atgctttatt tgtgaaattt gtgatgctat tgctttattt
     5641 gtaaccatta taagctgcaa taaacaagtt aacaacaaca attgcattca ttttatgttt
     5701 caggttcagg gggaggtgtg ggaggttttt taaagcaagt aaaacctcta caaatgtggt
     5761 atggctgatt atgatcctct agtacttctc gacaagcttt cagtcactgt cactatcagc
     5821 ttcactggaa tcagaagctg cagcttcact gccatcctcc tgggccgagt gctcgctgcc
     5881 actggggaag ggactggcgc tgcggctgtg gctccggctc cgctggccac ccccattgct
     5941 gccgctgtct gaatcattgt cactgccacc ttgggcctgt cctctgtcct catcatcaga
     6001 gtcggcatcg tcctcagaat cagcatcact gccaaagatc tcctctttgt cacgggcagc
     6061 ccgggcctca tcctcgctgc tctcgtcctc accactgcca ctcttgtcac tggcctcgtc
     6121 cctgtcacct tcctcccgtt cactctcgct gcccgagtgc tcatcttcac tgccctcctt
     6181 ctcactgctg ctgcccttct cctgctcctc atctgagccc ccagcttctt tctcttctgt
     6241 ctccatctcc tcttcctctt cctcctccgg ttcgtggttt tctagctggg ccttccgtgc
     6301 ctcctgagct tccagttcct tctcattcat gtcccgatgt ttgaccacaa gcagggcgtt
     6361 ggtgcctgac tgaaccccag ccttggcccg gcgcttacta aggcggaccc tggtttccaa
     6421 ctcattgtag taaaccccgt caccctctcg gaagatgaag aagtagtttt cctcatagcc
     6481 cttgctagct ttgttcttca cgttccagtt gtactcccga gcaattttgt agtcatacac
     6541 atcatctggt gcatagtcca tctcctcctc ctggtcccgc tttcgtttct tcaacgtctc
     6601 ttctacaggc aggaaatagg ccacaaactg gttcccttcc tcatccatca tgcccctaat
     6661 catggcctga gacatcatct ccaacgcagc tgcaccactc gtgtccttgg gggctgggtc
     6721 tgagtcaaag atcacctgag cacatggatt gatccacatc ttaaagtctg ggaagacagg
     6781 catgacctcc accggtgtga ctcggggttt gctgtaatgc tgtgagattg atttctgggc
     6841 atcctcaaaa gtcttctcaa tggctgtgat ctggctatcc ctgtctttgt atatttcttc
     6901 ctcggtaaac tgctgcttca cagaaacccc aatcttgacc tcaggcttct cattggagat
     6961 gccataacgg ttgaactcag tggagatgta ctctgtcttt cgcatccatg gcaccacctt
     7021 cgcgtgctgc tgggatctct tggagctggt gggggcctga atctcctctt ccaaaagttt
     7081 ctcatcagct ggatctagaa gaacattggg gtcgatgcgg taggtgtcag gattgatgag
     7141 atcgatggtg acccccaggt ctggctcagt caggaggtca tgtttgtgct gtttctccaa
     7201 ggaagtggct ttgtactgga cgaacctgtt ctggtcgaag gggtaggtga tgaacttggg
     7261 gtcgaagggg atatcaggga ggctattgca gtacttgact cggcagacca ctccagacct
     7321 ctcaggcaga gtccggtggg aattgggcct gtggccatcc tcccgctggg cctgggtctg
     7381 gatggtgggc gccatggatc cgcgcccgat ggtgggacgg tatgaataat ccggaatatt
     7441 tataggtttt tttattacaa aactgttacg aaaacagtaa aatacttatt tatttgcgag
     7501 atggttatca ttttaattat ctccatgatc tattaatatt ccggagtagg tcgatttaaa
     7561 ctaagctatg tgaaccgttt atttaaatgg ttatgatagt tattgctcag cggtggcagc
     7621 agccaactca gcttcctttc gggctttgtt agcagccgga tcttctaggc tcaagcagtg
     7681 atcagatcca gacatgataa gatacattga tgagtttgga caaaccacaa ctagaatgca
     7741 gtgaaaaaaa tgctttattt gtgaaatttg tgatgctatt gctttatttg taaccattat
     7801 aagctgcaat aaacaagtta acaacaacaa ttgcattcat tttatgtttc aggttcaggg
     7861 ggaggtgtgg gaggtttttt aaagcaagta aaacctctac aaatgtggta tggctgatta
     7921 tgatcctcta gtacttctcg acaagctttc aatcatcatc ttcttcctct tcatcgctga
     7981 tcacatactt cttatgcttt ttatttgctt tatcatcatc ttctgctttt ctctttccgg
     8041 aaggttcacc ttcctcatca ctggtaagtt tctttgcttt gagtaatctt tgagctttat
     8101 cttcttctga tccctcatca ctgtctgatg aatagattct ggctcgttcc tctcgaatgc
     8161 cccctttata tcggttttta atggcagcca agctgatgga ctcctcgcct tcctcctcct
     8221 catcgtatcg atcaggttcc aggtaactgg cgctcagccc ccgctggtgc tgtttctctc
     8281 tcattcggcg ctgctgagat tccctacgta tggaagccct caaacgttct tcttctttct
     8341 taatcatttc tgtgcgttgg cattcaggat cacgaccagc cattggcaag attctaatct
     8401 tctgtgtctt tgaacaccta tctgcaagtg acagagtcat ctttctatgt gtggcactgt
     8461 ccgtagagtg aggtctgaag gtgagtttcg ttttaaagac tgcttgtccc tgtagaccag
     8521 taccttgtct tataaaaaga tgattgtggt cgccctgcag tggggctttg tacacatcaa
     8581 acacttcatt gcctaaatgc agggacatgc ttccatctga ccacttgact atccgagcat
     8641 tgctttcttt aatttcattt ccttcttcat ctcggcgtat cctccatctt atagtatttt
     8701 ctacctttaa ttttaacctg gttctacctt cttcatccag catttcttca tcttcaaatt
     8761 catcttcata atactgagga tcaaaaggtc tgggctctac actgagaaag ttgggcagtt
     8821 taacaaaata taagtcgttt cctaaatcag tgtttacttt gggtatttct acttctattc
     8881 tggtctcagg aattggctcc tcttcctgtt gatcctgagg caatccattt tcatcaacag
     8941 gctgtcctgg agtaggtggt ttgtcttctc catcactccc tgaagagata tcatctgcac
     9001 ctccaaataa atccatggtt ccactattat cttttggcac ctcagtgtca ctatccgctt
     9061 ctgaatcaga tgcaatcgca ttcttgcgtt tcattcgtaa aacttcatct tcactatcac
     9121 tgcctcttgc agattctgat ttatgatcct gttcctcttc atcatctgaa tgcctgtgtt
     9181 cttcatctga ggcctgtggc ctttcatcat cagaattttg cattttctct tcatcagaca
     9241 gctgtggttg ttcttcatca tcagaattct gtttctcatc atcattatca gaagctaccg
     9301 gccgttcatc atcagaatta gccttttcct cctcagatag ctgttgtctc tcatcatcgg
     9361 aaagctgagg cctctcctca tcatctgtgt tctgcatttt ctcatcatcg tcagaattct
     9421 gcagcttatc ttcatcagat ccttgtgccc tctcctcatc atcagaattt tgtatctttt
     9481 catcatctga ctggtcactt ttatcttctc tgccccattt ttcatcatct gaatgtgctt
     9541 tttcagaacc ttctgcttct gaatgatggc tccctccatc cgatctatga ccttcgtctt
     9601 catcatcatt aggggcttct gatccactgt gctgatctac atctgagggg tcattgtcct
     9661 catggtcaga acgctcagaa gcttctgatc tattgtctga tctttcagag tgattatcac
     9721 taccactatg atgtgaagct ccctcgtcct cactgtcatc tccaaacagt tccttattac
     9781 ttggttgtcc tgaatcacct ctttcatcct gatcactttc acttccagag gcattactgc
     9841 cagaggcagc attctcttga tcagaatctg agtcagatcc agaatcagaa tctttacgct
     9901 cagcttcgct gtcggcgtcg ctcccgaaga gatcctccat atccgccatg gatccgcgcc
     9961 cgatggtggg acggtatgaa taatccggaa tatttatagg tttttttatt acaaaactgt
    10021 tacgaaaaca gtaaaatact tatttatttg cgagatggtt atcattttaa ttatctccat
    10081 gatctattaa tattccggag taggtcgatt taaacatcag gcatcattag gtttatttaa
    10141 atggttatga tagttattgc tcagcggtgg cagcagccaa ctcagcttcc tttcgggctt
    10201 tgttagcagc cggatcttct aggctcaagc agtgatcaga tccagacatg ataagataca
    10261 ttgatgagtt tggacaaacc acaactagaa tgcagtgaaa aaaatgcttt atttgtgaaa
    10321 tttgtgatgc tattgcttta tttgtaacca ttataagctg caataaacaa gttaacaaca
    10381 acaattgcat tcattttatg tttcaggttc agggggaggt gtgggaggtt ttttaaagca
    10441 agtaaaacct ctacaaatgt ggtatggctg attatgatcc tctagtactt ctcgacaagc
    10501 tttcagaatc tcaagtgcga tttatgcttt accatgtacc tgtccaatgt ttcccaaaac
    10561 cgtaagaaca ctggtctatc caaatgacgt ttgtgatagc tgagttctaa tactgttaca
    10621 tcccatttct gaacatttgg atccagacga acttcatcat acttcagatg gaaggcttta
    10681 attttagcaa atatatcaac tggtgatcca tcaggcaaaa gccatggcca acctttgaac
    10741 tgccatgcag gaccctgcac aaaaacggct acaacgcggt cccagtcttg aggcataagt
    10801 ttaaggggct ggtctactac tctataaggt actgtaacac taattgcagt gccccctggt
    10861 tgcatctggt cttttcttct ttgtattaga gtttcatttt ctcgttgaca accttgtttc
    10921 ttcttttcat ctgatgggac aaatttcagg tcctgtagaa ggtcttttgc attaagcatg
    10981 gttattaaag aggtggtagc tgcaggaatt atgataatgg gtgttcgaga tcctttcttc
    11041 tgatttgggg gaggtcttgc ttgagaaact ggtcttggta ctggctgggc tgcaggagtc
    11101 tgagtcttcc gggcagatgc accctcggca gctgggatag gctgtttggt gcgcaaagtg
    11161 ggatccacag gtgctgcatt tggggcaggt cgctgttcag gtgcacgccc ttcttctctg
    11221 gcttttacag attgaagaat tgcaaaaatg ttcttggaaa aattctttcc tgtgctttgt
    11281 aagatagttg ttcgtgtcct ccatactctc tctctgctga caatatctcg ggtcacatct
    11341 acctcagcat ccacaaaact cctctgttta agggcagtta tgtcatcatc tagatcagtc
    11401 ttgatagtag atcttttctt agccataatt ttggctttga ttgcagcaat tttttccact
    11461 gacatagctt cagacaaaga cctaatctgt tcagtctgta caatcccttc tttgtgaccc
    11521 tccaaacggg cagccaatct ctctttatca aggcgcacac actcttcatc ctcaattcgt
    11581 ggtttctttg cttctgctaa aacttcatct gcagctcgtt tgacttgagt agatcgctga
    11641 agacctattt ctaagggagc gcttctgtct atacttgccg atgttgacgc ttcaccattg
    11701 agatatccaa gtagatcttt tcgatcaggt cttctaacca caggaatatt ttcagtagct
    11761 gcacgtcgga cataaacagg atgagaaagg tgcacgttat taagtagaaa taaaatggaa
    11821 tccaatgtgt agtactctct gggttggcct tcctttccag tcccccaaac aacatagttg
    11881 gtcttcacat tcttgggcca ggagaactcc ccgaagatca cttcgtctcc cttcaccaca
    11941 atctccttct tctggatgtt gtactgtcgc aggacgctaa gcacgtccgc catgggtccc
    12001 tgaaagagga cttcaagatc gagcttgtca tcgtcgtcct tgtagtccat ggatccgcgc
    12061 ccgatggtgg gacggtatga ataatccgga atatttatag gtttttttat tacaaaactg
    12121 ttacgaaaac agtaaaatac ttatttattt gcgagatggt tatcatttta attatctcca
    12181 tgatctatta atattccgga gtaggtcgat ttaaacgtgc aatagtatcc agtttattta
    12241 aatggttatg atagttattg ctcagcggtg gcagcagcca actcagcttc ctttcgggct
    12301 ttgttagcag ccggatcttc taggctcaag cagtgatcag atccagacat gataagatac
    12361 attgatgagt ttggacaaac cacaactaga atgcagtgaa aaaaatgctt tatttgtgaa
    12421 atttgtgatg ctattgcttt atttgtaacc attataagct gcaataaaca agttaacaac
    12481 aacaattgca ttcattttat gtttcaggtt cagggggagg tgtgggaggt tttttaaagc
    12541 aagtaaaacc tctacaaatg tggtatggct gattatgatc ctctagtact tctcgacaag
    12601 ctttcacttt tcgaactgcg ggtggctcca gggtccctga aagaggactt caagtgcacc
    12661 gtcactatca tctgacccat gttctgagcc tctatctgag gcctcattgt tggatccctc
    12721 tggttccgat tcatttccag agccttggcc agaaccctca ttatcagatc ctctctccga
    12781 ttcgtgatct gattcggcac ttggagaagc tgggcgagag tcgttctcag aaactcctga
    12841 gtggcttctc cctgactgca cagattcatt gtcagactgc tcagaaccgg agggccttct
    12901 ctttctggat ggctggtcac tgtctgagtc ctgatctgac cgctgtcttc gtggcctccg
    12961 gggactgccg gcctcgctgc cagacttgtt ctggttctca tcggaatcac tctctgatga
    13021 ggcacatttc tttcgttgtt cgtcctcgtc tgagtcactg ttgctgttgc tgttcctggg
    13081 atgtccttca tcagcaattt taagtttatc ctcatccgaa gagtcatcac ttgatgaaat
    13141 tatggctttg gattttattt ttcccttcat tgatggaggc agacgttctg gcttgggagc
    13201 ctttttcttc tctgcttttg gtggacgtcg tttttttggt ttggggccat tttctgtttc
    13261 atcatcatca gatccttctt ctccctttgg atgtcttctc ctctttttct tctttctctc
    13321 accaccctcc tcatcttcac cttcttgttc actgccacta ccctttcttc tcttcttctt
    13381 tttggatata ggtaggtcat catcagtgtc atcattgaca aattcatcaa actctcctcc
    13441 cttcttagaa cgccgtccac caccaccacc tcttttcttc tcttttgttg cttcagtctc
    13501 accagtaaac ataagaatat ttttggtctt ctccacatac tgggcccgct gttccaaaag
    13561 tttcttttgc tcttcctttt ctctgagacg tttctcttcc tgttctttaa gaagtttctg
    13621 ccttaacagc tccttttctt gctcttgctt ggcccgcagc tcccgctctt cttcatcttg
    13681 tttgcgtgcc cgggccacat ggtactgggc ctggctcagt aagtcagaac actgcctggc
    13741 ttctgtagca gcaagggcca aatcaaatct cattttatct cccactttac tcaaataact
    13801 gaagtatcta tgtgcaagct ccagttcttt cacagcatta agtacttcct tcagattact
    13861 tttttcatct ttcaggacag aggtagctaa tctttgcagg accaaggcca cattaaacat
    13921 aagaactgta tcactgggtg ccacatgtct agccttcagc aaagtctgtt tgcattcctg
    13981 taacttgcca cacttgaaga gggcccgggc caaatagagt acaacttcag tgttttggtg
    14041 cttatagaac tttcggaggc agttttcata catctgaacg gcgctgatgt actgcttttg
    14101 ctccacatag atgtgtgcta agttcagcca cacatcacta atatctgctg ttgcttctct
    14161 tacttgggca aatacatcac gagcttcacg aaaatatcct ttgtgggcca aaacagctcc
    14221 tatgccattg gcagcataca gattctttgc atcatttctg agtacttgtt tgtagatggc
    14281 cagagcacga tcttgatgac gcttttcctt ttctcgatct cgggtgggct gatgtaaagt
    14341 ttggagccac acgttgccaa gggctagcat agaataggta tcactctgtg tggatggctg
    14401 ttttaatatc ctctcaaact tcttctgccc aggaccccat tcttgttttg ccaaatgaag
    14461 attgccaatc aaagaccaag catctggatg atcctgatta atctgaagag cttccttaaa
    14521 ccaatctgaa gcctcataaa agtttccctt atctctagcc atggctccta ggcgcaaata
    14581 gcagtcaaca taattaggat gttcgcgtaa gatgttttta tacagttttt ctgcttcatg
    14641 gaattcacac atcgcctcat atagcctggc gagattatat gacgtagtaa cggaaatggc
    14701 gttatagtaa tgctcatcgt gttccgcttc tgcttttgca cggtccaatg acgccaaaaa
    14761 atatttctta gcctccccta ggtttccaag tctaaaatgg agggcaccca cattattgag
    14821 aatctctgga ggaacatcgg cctgcacttt ctcctgaagg attcgtgttg ctgttccata
    14881 ggctgaaagg gcaccctgta tatcagtctg ttctaagatt tgtgccaatt caatccaagc
    14941 ttcaacatca tcgggatact gttctgtgac cttcttcaaa tggcccttgg caatatctcg
    15001 tttttcttga tcttctgagg cagcatagag agagccgaga attttcatag tttcgtaatt
    15061 attaggataa gctttcaaaa ccttctcaaa gcactgagat gcattttctt tgtcacctcg
    15121 ataaatatac atttgtccca aaccaaaaaa tgggagcaca aaagaggatg aggcaaactg
    15181 tgtggcttga taatagtact gaaaagcttg gtcataatct tcctgaacat ggaatgatct
    15241 agctagctga tagcagctct ctgcttgcat agcttccact tctgtattat ggaatgcatg
    15301 gagggccaga tgctggactt tactataatc ctttttgaag aaaaagtgat ttgccaaatg
    15361 gttcaatacc atagggttgc taggatcaat agtataggct ctggaaagaa gctggacacc
    15421 atttttaatg gaatcagcct ctttattgtt gagttctaga acagccagtc caaccaatgc
    15481 tcccacgcat ttggaattga gttccagggc tctgctgaat gccagacgag ctttttccag
    15541 tttgttaagt ttcacaaagc aatgacccat tcctaaacga acttccgctg gacatcctgg
    15601 gttagtacgc aatgctttct tatagtaagc aagagctcct ctgtaatcct tcttgttgaa
    15661 ggaaatgcaa gctttaccaa gaagggctgg aatattattt ggagactgat tgagtacaaa
    15721 atgaaactgt gcatcagctt gatccatttt gtcaccctca agtaggcaga agcaggctct
    15781 tcccaacaaa tggttctgat catacataat aattttatcg gccattgtat acaacaaggt
    15841 ggcctgtgta ataagatcct ttttattgtc cttattcttt tctttccgag cctgttgtac
    15901 ataatacgct gccaatgtat ccaagcaagt catctggtct ttttcatggt ctctatagtc
    15961 caaattgcca tctatacgtg ctgcttccaa caattttaca aactcttctg tttttccttg
    16021 cttgtagtat tccagcgcca aagcaatcca tatgtgcagt tgtgtgtgtt cctgtttcag
    16081 aatactgata acttcatctc cctccggtaa ctgatcgaag tcaagttcaa tgacctcgtc
    16141 agtgtcccgg aggggaatct cgatggagcc ccgcgacatg gatccgcgcc cgatggtggg
    16201 acggtatgaa taatccggaa tatttatagg tttttttatt acaaaactgt tacgaaaaca
    16261 gtaaaatact tatttatttg cgagatggtt atcattttaa ttatctccat gatctattaa
    16321 tattccggag taggtcgatt taaatcttta gaccatagag cgttcacttg acagagtgcc
    16381 agccctggga ccgaaccccg cgtttatgaa caaacgaccc aacacccgtg cgttttattc
    16441 tgtcttttta ttgccgtcat agcgcgggtt ccttccggta ttgtctcctt ccgtgtttca
    16501 gttagcctcg ataaaaactt tagaccatag agcgtttaaa cccagataac ttcgtataat
    16561 gtatgctata cgaagttatg gtaccgcggc cgcgtagagg atctgttgat cagcagttca
    16621 acctgttgat agtacaaaca aagctgtagc caaccactag aactatagct agagtcctgg
    16681 gcgaacaaac gatgctcgcc ttccagaaaa ccgaggatgc gaaccacttc atccggggtc
    16741 agcaccaccg gcaagcgccg cgacggccga ggtctaccga tctcctgaag caagggcaga
    16801 tccgtgcaca gcaccttgcc gtagaagaac agcaaggccg ccaatgcctg acgatgcgtg
    16861 gagaccgaaa ccttgcgctc gttcgccagc caggacagaa atgcctcgac ttcgctgctg
    16921 cccaaggttg ccgggtgacg cacaccgtgg aaacggatga aggcacaaac ccagttgaca
    16981 taagcctgtt cggttcgtaa actgtaatgc aagtagcgta tgcgctcacg caactggtcc
    17041 agaaccttga ccgaacgcag cggtggtaac ggcgcagtgg cggttttcat ggcttgttat
    17101 gactgttttt ttgtacagtc tatgcctcgg gcatccaagc agcaagcgcg ttacgccgtg
    17161 ggtcgatgtt tgatgttatg gagcagcaac gatgttacgc agcagcaacg atgttacgca
    17221 gcagggcagt cgccctaaaa caaagttagg tggctcaagt atgggcatca ttcgcacatg
    17281 taggctcggc cctgaccaag tcaaatccat gcgggctgct cttgatcttt tcggtcgtga
    17341 gttcggagac gtagccacct actcccaaca tcagccggac tccgattacc tcgggaactt
    17401 gctccgtagt aagacattca tcgcgcttgc tgccttcgac caagaagcgg ttgttggcgc
    17461 tctcgcggct tacgttctgc ccaagtttga gcagccgcgt agtgagatct atatctatga
    17521 tctcgcagtc tccggcgagc accggaggca gggcattgcc accgcgctca tcaatctcct
    17581 caagcatgag gccaacgcgc ttggtgctta tgtgatctac gtgcaagcag attacggtga
    17641 cgatcccgca gtggctctct atacaaagtt gggcatacgg gaagaagtga tgcactttga
    17701 tatcgaccca agtaccgcca cctaacaatt cgttcaagcc gagatcggct tcccggccgc
    17761 ggagttgttc ggtaaattgt cacaacgccg cgaatatagt ctttaccatg cccttggcca
    17821 cgcccctctt taatacgacg ggcaatttgc acttcagaaa atgaagagtt tgctttagcc
    17881 ataacaaaag tccagtatgc tttttcacag cataactgga ctgatttcag tttacaacta
    17941 ttctgtctag tttaagactt tattgtcata gtttagatct attttgttca gtttaagact
    18001 ttattgtccg cccacacccg cttacgcagg gcatccattt attactcaac cgtaaccgat
    18061 tttgccaggt tacgcggctg gtctatgcgg tgtgaaatac cgcacagatg cgtaaggaga
    18121 aaataccgca tcaggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt
    18181 cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca
    18241 ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccg
//
