LOCUS       sequence-463632-       16929 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Protein expression in insect cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 16929)
  AUTHORS   Lopez Martinez D, Todorovski I, Noe Gonzalez M, Rusimbi C, Blears D,
            Khallou N, Han Z, Dirac-Svejstrup AB, Svejstrup JQ
  TITLE     PAF1C-mediated activation of CDK12/13 kinase activity is critical 
            for CTD phosphorylation and transcript elongation.
  JOURNAL   Mol Cell. 2025 May 15;85(10):1952-1967.e8. doi: 
            10.1016/j.molcel.2025.04.012. Epub 2025 May 1.
  PUBMED    40315851
REFERENCE   2  (bases 1 to 16929)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2025.04.012"; journalName: "Mol Cell"; date: 
            "2025-05-15- 15"; volume: "85"; issue: "10"; pages: "1952-1967.e8"
COMMENT     Sequence Label: pBIG1a-PAF1CdeltaWDR61
FEATURES             Location/Qualifiers
     source          1..16929
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    1498..1632
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     mobile_element  1947..2112
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     CDS             2636..3427
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      3468..3922
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3555..3574)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3764..3785
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        3948..4052
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4053..4121,4122..4913)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4271..4290)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5084..5672
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5573..5592
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5826..5843
                     /label=L4440
                     /note="L4440 vector, forward primer"
     mobile_element  5977..6201
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     CDS             complement(6268..6801)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(join(6990..6995,6996..7012,7013..7018))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     protein_bind    complement(7414..7447)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     complement(7493..7512)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    7537..7585
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     promoter        7679..7770
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     CDS             11338..11361
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             11362..11385
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     polyA_signal    11518..11652
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        11808..11899
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     CDS             11945..11968
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             11975..11998
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     polyA_signal    13724..13858
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        14014..14105
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     polyA_signal    16278..16412
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        16568..16659
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
ORIGIN
        1 gagccagacc tgggggtcac catcgatctc atcaatcctg acacctaccg catcgacccc
       61 aatgttcttc tagatccagc tgatgagaaa cttttggaag aggagattca ggcccccacc
      121 agctccaaga gatcccagca gcacgcgaag gtggtgccat ggatgcgaaa gacagagtac
      181 atctccactg agttcaaccg ttatggcatc tccaatgaga agcctgaggt caagattggg
      241 gtttctgtga agcagcagtt taccgaggaa gaaatataca aagacaggga tagccagatc
      301 acagccattg agaagacttt tgaggatgcc cagaaatcaa tctcacagca ttacagcaaa
      361 ccccgagtca caccggtgga ggtcatgcct gtcttcccag actttaagat gtggatcaat
      421 ccatgtgctc aggtgatctt tgactcagac ccagccccca aggacacgag tggtgcagct
      481 gcgttggaga tgatgtctca ggccatgatt aggggcatga tggatgagga agggaaccag
      541 tttgtggcct atttcctgcc tgtagaagag acgttgaaga aacgaaagcg ggaccaggag
      601 gaggagatgg actatgcacc agatgatgtg tatgactaca aaattgctcg ggagtacaac
      661 tggaacgtga agaacaaagc tagcaagggc tatgaggaaa actacttctt catcttccga
      721 gagggtgacg gggtttacta caatgagttg gaaaccaggg tccgccttag taagcgccgg
      781 gccaaggctg gggttcagtc aggcaccaac gccctgcttg tggtcaaaca tcgggacatg
      841 aatgagaagg aactggaagc tcaggaggca cggaaggccc agctagaaaa ccacgaaccg
      901 gaggaggaag aggaagagga gatggagaca gaagagaaag aagctggggg ctcagatgag
      961 gagcaggaga agggcagcag cagtgagaag gagggcagtg aagatgagca ctcgggcagc
     1021 gagagtgaac gggaggaagg tgacagggac gaggccagtg acaagagtgg cagtggtgag
     1081 gacgagagca gcgaggatga ggcccgggct gcccgtgaca aagaggagat ctttggcagt
     1141 gatgctgatt ctgaggacga tgccgactct gatgatgagg acagaggaca ggcccaaggt
     1201 ggcagtgaca atgattcaga cagcggcagc aatgggggtg gccagcggag ccggagccac
     1261 agccgcagcg ccagtccctt ccccagtggc agcgagcact cggcccagga ggatggcagt
     1321 gaagctgcag cttctgattc cagtgaagct gatagtgaca gtgactgaaa gcttgtcgag
     1381 aagtactaga ggatcataat cagccatacc acatttgtag aggttttact tgctttaaaa
     1441 aacctcccac acctccccct gaacctgaaa cataaaatga atgcaattgt tgttgttaac
     1501 ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat
     1561 aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat
     1621 catgtctgga tctgatcact gcttgagcct agaagatccg gctgctaaca aagcccgaaa
     1681 ggaagctgag ttggctgctg ccaccgctga gcaataacta tcataaccat ttaaataaat
     1741 ctatatctca atcggggtta cgtcgatacc ccaccgagac cccattggga ccaatacgcc
     1801 cgcgtttctt ccttttcccc accccaaccc ccaagttcgg gtgaaggccc agggctcgca
     1861 gccaacgtcg gggcggcaag ccctgccata gccactacgg gttaattaaa aactatatct
     1921 caatcggggt ttaaacagga gatccgaacc agataagtga aatctagttc caaactattt
     1981 tgtcattttt aattttcgta ttagcttacg acgctacacc cagttcccat ctattttgtc
     2041 actcttccct aaataatcct taaaaactcc atttccaccc ctcccagttc ccaactattt
     2101 tgtccgccca cagcggggca tttttcttcc tgttatgttt ttaatcaaac atcctgccaa
     2161 ctccatgtga caaaccgtca tcttcggcta ctttttctct gtcacagaat gaaaattttt
     2221 ctgtcatctc ttcgttatta atgtttgtaa ttgactgaat atcaacgctt atttgcagcc
     2281 tgaactggga ggcagaataa atgatcatat cgtcaattat tacctccacg gggagagcct
     2341 gagcaaactg gcctcaggca tttgagaagc acacggtcac actgcttccg gtagtcaata
     2401 aaccggtaag tagcgtatgc gctcacgcaa ctggtccaga accttgaccg aacgcagcgg
     2461 tggtaacggc gcagtggcgg ttttcatggc ttgttatgac tgtttttttg gggtacagtc
     2521 tatgcctcgg gcatccaagc agcaagcgcg ttacgccgtg ggtcgatgtt tgatgttatg
     2581 gagcagcaac gatgttacgc agcagggcag tcgccctaaa acaaagttaa acatcatgag
     2641 ggaagcggtg atcgccgaag tatcgactca actatcagag gtagttggcg tcatcgagcg
     2701 ccatctcgaa ccgacgttgc tggccgtaca tttgtacggc tccgcagtgg atggcggcct
     2761 gaagccacac agtgatattg atttgctggt tacggtgacc gtaaggcttg atgaaacaac
     2821 gcggcgagct ttgatcaacg accttttgga aacttcggct tcccctggag agagcgagat
     2881 tctccgcgct gtagaagtca ccattgttgt gcacgacgac atcattccgt ggcgttatcc
     2941 agctaagcgc gaactgcaat ttggagaatg gcagcgcaat gacattcttg caggtatctt
     3001 cgagccagcc acgatcgaca ttgatctggc tatcttgctg acaaaagcaa gagaacatag
     3061 cgttgccttg gtaggtccag cggcggagga actctttgat ccggttcctg aacaggatct
     3121 atttgaggcg ctaaatgaaa ccttaacgct atggaactcg ccgcccgact gggctggcga
     3181 tgagcgaaat gtagtgctta cgttgtcccg catttggtac agcgcagtaa ccggcaaaat
     3241 cgcgccgaag gatgtcgctg ccgactgggc aatggagcgc ctgccggccc agtatcagcc
     3301 cgtcatactt gaagctagac aggcttatct tggacaagaa gaagatcgct tggcctcgcg
     3361 cgcagatcag ttggaagaat ttgtccacta cgtgaaaggc gagatcacca aggtagtcgg
     3421 caaataatgt ctaacaattc gttcaagccg acggattggc gaatgggacg cgccctgtag
     3481 cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag
     3541 cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt
     3601 tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca
     3661 cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata
     3721 gacggttttt cgccctttga cgttggagtc cacgttctta atagtggact cttgttccaa
     3781 actggaacaa cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg
     3841 atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattttaac
     3901 aaaatattaa cgcttacaat ttaggtggca cttttcgggg aaatgtgcgc ggaaccccta
     3961 tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat
     4021 aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc
     4081 ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga
     4141 aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca
     4201 acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt
     4261 ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg
     4321 gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc
     4381 atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata
     4441 acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt
     4501 tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag
     4561 ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca
     4621 aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg
     4681 aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg
     4741 ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag
     4801 atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg
     4861 aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag
     4921 accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga
     4981 tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt
     5041 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc
     5101 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc
     5161 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac
     5221 caaatactat tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac
     5281 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt
     5341 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct
     5401 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat
     5461 acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt
     5521 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg
     5581 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt
     5641 gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt
     5701 tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg
     5761 tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg
     5821 agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta
     5881 cgcatctgtg cggtatttca caccgcatag accagccgcg taacctggca aaatcggtta
     5941 cggttgagta ataaatggat gccctgcgta agcgggtgtg ggcggacaat aaagtcttaa
     6001 actgaacaaa atagatctaa actatgacaa taaagtctta aactagacag aatagttgta
     6061 aactgaaatc agtccagtta tgctgtgaaa aagcatactg gacttttgtt atggctaaag
     6121 caaactcttc attttctgaa gtgcaaattg cccgtcgtat taaagagggg cgtggccaag
     6181 ggcatggtaa agactatatt cgcggcgttg tgacaattta ccgaacaact ccgcggccgg
     6241 gaagccgatc tcggcttgaa cgaattgtta ggtggcggta cttgggtcga tatcaaagtg
     6301 catcacttct tcccgtatgc ccaactttgt atagagagcc actgcgggat cgtcaccgta
     6361 atctgcttgc acgtagatca cataagcacc aagcgcgttg gcctcatgct tgaggagatt
     6421 gatgagcgcg gtggcaatgc cctgcctccg gtgctcgccg gagactgcga gatcatagat
     6481 atagatctca ctacgcggct gctcaaactt gggcagaacg taagccgcga gagcgccaac
     6541 aaccgcttct tggtcgaagg cagcaagcgc gatgaatgtc ttactacgga gcaagttccc
     6601 gaggtaatcg gagtccggct gatgttggga gtaggtggct acgtctccga actcacgacc
     6661 gaaaagatca agagcagccc gcatggattt gacttggtca gggccgagcc tacatgtgcg
     6721 aatgatgccc atacttgagc cacctaactt tgttttaggg cgactgccct gctgcgtaac
     6781 atcgttgctg ctgcgtaaca tcgttgctgc tccataacat caaacatcga cccacggcgt
     6841 aacgcgcttg ctgcttggat gcccgaggca tagactgtac aaaaaaacag tcataacaag
     6901 ccatgaaaac cgccactgcg ccgttaccac cgctgcgttc ggtcaaggtt ctggaccagt
     6961 tgcgtgagcg catacgctac ttgcattaca gtttacgaac cgaacaggct tatgtcaact
     7021 gggtttgtgc cttcatccgt ttccacggtg tgcgtcaccc ggcaaccttg ggcagcagcg
     7081 aagtcgaggc atttctgtcc tggctggcga acgagcgcaa ggtttcggtc tccacgcatc
     7141 gtcaggcatt ggcggccttg ctgttcttct acggcaaggt gctgtgcacg gatctgccct
     7201 tgcttcagga gatcggtaga cctcggccgt cgcggcgctt gccggtggtg ctgaccccgg
     7261 atgaagtggt tcgcatcctc ggttttctgg aaggcgagca tcgtttgttc gcccaggact
     7321 ctagctatag ttctagtggt tggctacagc tttgtttgta ctatcaacag gttgaactgc
     7381 tgatcaacag atcctctacg cggccgcggt accataactt cgtatagcat acattatacg
     7441 aagttatctg ggtttaaacg ctctatggtc taaagttttt atcgaggcta actgaaacac
     7501 ggaaggagac aataccggaa ggaacccgcg ctatgacggc aataaaaaga cagaataaaa
     7561 cgcacgggtg ttgggtcgtt tgttcataaa cgcggggttc ggtcccaggg ctggcactct
     7621 gtcaagtgaa cgctctatgg tctaaagatt taaatgacct actccggaat attaatagat
     7681 catggagata attaaaatga taaccatctc gcaaataaat aagtatttta ctgttttcgt
     7741 aacagttttg taataaaaaa acctataaat attccggatt attcataccg tcccaccatc
     7801 gggcgcggat ccatgtcgcg gggctccatc gagattcccc tccgggacac tgacgaggtc
     7861 attgaacttg acttcgatca gttaccggag ggagatgaag ttatcagtat tctgaaacag
     7921 gaacacacac aactgcacat atggattgct ttggcgctgg aatactacaa gcaaggaaaa
     7981 acagaagagt ttgtaaaatt gttggaagca gcacgtatag atggcaattt ggactataga
     8041 gaccatgaaa aagaccagat gacttgcttg gatacattgg cagcgtatta tgtacaacag
     8101 gctcggaaag aaaagaataa ggacaataaa aaggatctta ttacacaggc caccttgttg
     8161 tatacaatgg ccgataaaat tattatgtat gatcagaacc atttgttggg aagagcctgc
     8221 ttctgcctac ttgagggtga caaaatggat caagctgatg cacagtttca ttttgtactc
     8281 aatcagtctc caaataatat tccagccctt cttggtaaag cttgcatttc cttcaacaag
     8341 aaggattaca gaggagctct tgcttactat aagaaagcat tgcgtactaa cccaggatgt
     8401 ccagcggaag ttcgtttagg aatgggtcat tgctttgtga aacttaacaa actggaaaaa
     8461 gctcgtctgg cattcagcag agccctggaa ctcaattcca aatgcgtggg agcattggtt
     8521 ggactggctg ttctagaact caacaataaa gaggctgatt ccattaaaaa tggtgtccag
     8581 cttctttcca gagcctatac tattgatcct agcaacccta tggtattgaa ccatttggca
     8641 aatcactttt tcttcaaaaa ggattatagt aaagtccagc atctggccct ccatgcattc
     8701 cataatacag aagtggaagc tatgcaagca gagagctgct atcagctagc tagatcattc
     8761 catgttcagg aagattatga ccaagctttt cagtactatt atcaagccac acagtttgcc
     8821 tcatcctctt ttgtgctccc attttttggt ttgggacaaa tgtatattta tcgaggtgac
     8881 aaagaaaatg catctcagtg ctttgagaag gttttgaaag cttatcctaa taattacgaa
     8941 actatgaaaa ttctcggctc tctctatgct gcctcagaag atcaagaaaa acgagatatt
     9001 gccaagggcc atttgaagaa ggtcacagaa cagtatcccg atgatgttga agcttggatt
     9061 gaattggcac aaatcttaga acagactgat atacagggtg ccctttcagc ctatggaaca
     9121 gcaacacgaa tccttcagga gaaagtgcag gccgatgttc ctccagagat tctcaataat
     9181 gtgggtgccc tccattttag acttggaaac ctaggggagg ctaagaaata ttttttggcg
     9241 tcattggacc gtgcaaaagc agaagcggaa cacgatgagc attactataa cgccatttcc
     9301 gttactacgt catataatct cgccaggcta tatgaggcga tgtgtgaatt ccatgaagca
     9361 gaaaaactgt ataaaaacat cttacgcgaa catcctaatt atgttgactg ctatttgcgc
     9421 ctaggagcca tggctagaga taagggaaac ttttatgagg cttcagattg gtttaaggaa
     9481 gctcttcaga ttaatcagga tcatccagat gcttggtctt tgattggcaa tcttcatttg
     9541 gcaaaacaag aatggggtcc tgggcagaag aagtttgaga ggatattaaa acagccatcc
     9601 acacagagtg atacctattc tatgctagcc cttggcaacg tgtggctcca aactttacat
     9661 cagcccaccc gagatcgaga aaaggaaaag cgtcatcaag atcgtgctct ggccatctac
     9721 aaacaagtac tcagaaatga tgcaaagaat ctgtatgctg ccaatggcat aggagctgtt
     9781 ttggcccaca aaggatattt tcgtgaagct cgtgatgtat ttgcccaagt aagagaagca
     9841 acagcagata ttagtgatgt gtggctgaac ttagcacaca tctatgtgga gcaaaagcag
     9901 tacatcagcg ccgttcagat gtatgaaaac tgcctccgaa agttctataa gcaccaaaac
     9961 actgaagttg tactctattt ggcccgggcc ctcttcaagt gtggcaagtt acaggaatgc
    10021 aaacagactt tgctgaaggc tagacatgtg gcacccagtg atacagttct tatgtttaat
    10081 gtggccttgg tcctgcaaag attagctacc tctgtcctga aagatgaaaa aagtaatctg
    10141 aaggaagtac ttaatgctgt gaaagaactg gagcttgcac atagatactt cagttatttg
    10201 agtaaagtgg gagataaaat gagatttgat ttggcccttg ctgctacaga agccaggcag
    10261 tgttctgact tactgagcca ggcccagtac catgtggccc gggcacgcaa acaagatgaa
    10321 gaagagcggg agctgcgggc caagcaagag caagaaaagg agctgttaag gcagaaactt
    10381 cttaaagaac aggaagagaa acgtctcaga gaaaaggaag agcaaaagaa acttttggaa
    10441 cagcgggccc agtatgtgga gaagaccaaa aatattctta tgtttactgg tgagactgaa
    10501 gcaacaaaag agaagaaaag aggtggtggt ggtggacggc gttctaagaa gggaggagag
    10561 tttgatgaat ttgtcaatga tgacactgat gatgacctac ctatatccaa aaagaagaag
    10621 agaagaaagg gtagtggcag tgaacaagaa ggtgaagatg aggagggtgg tgagagaaag
    10681 aagaaaaaga ggagaagaca tccaaaggga gaagaaggat ctgatgatga tgaaacagaa
    10741 aatggcccca aaccaaaaaa acgacgtcca ccaaaagcag agaagaaaaa ggctcccaag
    10801 ccagaacgtc tgcctccatc aatgaaggga aaaataaaat ccaaagccat aatttcatca
    10861 agtgatgact cttcggatga ggataaactt aaaattgctg atgaaggaca tcccaggaac
    10921 agcaacagca acagtgactc agacgaggac gaacaacgaa agaaatgtgc ctcatcagag
    10981 agtgattccg atgagaacca gaacaagtct ggcagcgagg ccggcagtcc ccggaggcca
    11041 cgaagacagc ggtcagatca ggactcagac agtgaccagc catccagaaa gagaaggccc
    11101 tccggttctg agcagtctga caatgaatct gtgcagtcag ggagaagcca ctcaggagtt
    11161 tctgagaacg actctcgccc agcttctcca agtgccgaat cagatcacga atcggagaga
    11221 ggatctgata atgagggttc tggccaaggc tctggaaatg aatcggaacc agagggatcc
    11281 aacaatgagg cctcagatag aggctcagaa catgggtcag atgatagtga cggtgcactt
    11341 gaagtcctct ttcagggacc ctggagccac ccgcagttcg aaaagtgaaa gcttgtcgag
    11401 aagtactaga ggatcataat cagccatacc acatttgtag aggttttact tgctttaaaa
    11461 aacctcccac acctccccct gaacctgaaa cataaaatga atgcaattgt tgttgttaac
    11521 ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat
    11581 aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat
    11641 catgtctgga tctgatcact gcttgagcct agaagatccg gctgctaaca aagcccgaaa
    11701 ggaagctgag ttggctgctg ccaccgctga gcaataacta tcataaccat ttaaataaac
    11761 tggatactat tgcacgttta aatcgaccta ctccggaata ttaatagatc atggagataa
    11821 ttaaaatgat aaccatctcg caaataaata agtattttac tgttttcgta acagttttgt
    11881 aataaaaaaa cctataaata ttccggatta ttcataccgt cccaccatcg ggcgcggatc
    11941 catggactac aaggacgacg atgacaagct cgatcttgaa gtcctctttc agggacccat
    12001 ggcggacgtg cttagcgtcc tgcgacagta caacatccag aagaaggaga ttgtggtgaa
    12061 gggagacgaa gtgatcttcg gggagttctc ctggcccaag aatgtgaaga ccaactatgt
    12121 tgtttggggg actggaaagg aaggccaacc cagagagtac tacacattgg attccatttt
    12181 atttctactt aataacgtgc acctttctca tcctgtttat gtccgacgtg cagctactga
    12241 aaatattcct gtggttagaa gacctgatcg aaaagatcta cttggatatc tcaatggtga
    12301 agcgtcaaca tcggcaagta tagacagaag cgctccctta gaaataggtc ttcagcgatc
    12361 tactcaagtc aaacgagctg cagatgaagt tttagcagaa gcaaagaaac cacgaattga
    12421 ggatgaagag tgtgtgcgcc ttgataaaga gagattggct gcccgtttgg agggtcacaa
    12481 agaagggatt gtacagactg aacagattag gtctttgtct gaagctatgt cagtggaaaa
    12541 aattgctgca atcaaagcca aaattatggc taagaaaaga tctactatca agactgatct
    12601 agatgatgac ataactgccc ttaaacagag gagttttgtg gatgctgagg tagatgtgac
    12661 ccgagatatt gtcagcagag agagagtatg gaggacacga acaactatct tacaaagcac
    12721 aggaaagaat ttttccaaga acatttttgc aattcttcaa tctgtaaaag ccagagaaga
    12781 agggcgtgca cctgaacagc gacctgcccc aaatgcagca cctgtggatc ccactttgcg
    12841 caccaaacag cctatcccag ctgcctataa cagatacgat caggaaagat tcaaaggaaa
    12901 agaagaaacg gaaggcttca aaattgacac tatgggaacc taccatggta tgacactgaa
    12961 atctgtaacg gagggtgcat ctgcccggaa gactcagact cctgcagccc agccagtacc
    13021 aagaccagtt tctcaagcaa gacctccccc aaatcagaag aaaggatctc gaacacccat
    13081 tatcataatt cctgcagcta ccacctcttt aataaccatg cttaatgcaa aagaccttct
    13141 acaggacctg aaatttgtcc catcagatga aaagaagaaa caaggttgtc aacgagaaaa
    13201 tgaaactcta atacaaagaa gaaaagacca gatgcaacca gggggcactg caattagtgt
    13261 tacagtacct tatagagtag tagaccagcc ccttaaactt atgcctcaag actgggaccg
    13321 cgttgtagcc gtttttgtgc agggtcctgc atggcagttc aaaggttggc catggctttt
    13381 gcctgatgga tcaccagttg atatatttgc taaaattaaa gccttccatc tgaagtatga
    13441 tgaagttcgt ctggatccaa atgttcagaa atgggatgta acagtattag aactcagcta
    13501 tcacaaacgt catttggata gaccagtgtt cttacggttt tgggaaacat tggacaggta
    13561 catggtaaag cataaatcgc acttgagatt ctgaaagctt gtcgagaagt actagaggat
    13621 cataatcagc cataccacat ttgtagaggt tttacttgct ttaaaaaacc tcccacacct
    13681 ccccctgaac ctgaaacata aaatgaatgc aattgttgtt gttaacttgt ttattgcagc
    13741 ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc
    13801 actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggatctg
    13861 atcactgctt gagcctagaa gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg
    13921 ctgctgccac cgctgagcaa taactatcat aaccatttaa ataaacctaa tgatgcctga
    13981 tgtttaaatc gacctactcc ggaatattaa tagatcatgg agataattaa aatgataacc
    14041 atctcgcaaa taaataagta ttttactgtt ttcgtaacag ttttgtaata aaaaaaccta
    14101 taaatattcc ggattattca taccgtccca ccatcgggcg cggatccatg gcggatatgg
    14161 aggatctctt cgggagcgac gccgacagcg aagctgagcg taaagattct gattctggat
    14221 ctgactcaga ttctgatcaa gagaatgctg cctctggcag taatgcctct ggaagtgaaa
    14281 gtgatcagga tgaaagaggt gattcaggac aaccaagtaa taaggaactg tttggagatg
    14341 acagtgagga cgagggagct tcacatcata gtggtagtga taatcactct gaaagatcag
    14401 acaatagatc agaagcttct gagcgttctg accatgagga caatgacccc tcagatgtag
    14461 atcagcacag tggatcagaa gcccctaatg atgatgaaga cgaaggtcat agatcggatg
    14521 gagggagcca tcattcagaa gcagaaggtt ctgaaaaagc acattcagat gatgaaaaat
    14581 ggggcagaga agataaaagt gaccagtcag atgatgaaaa gatacaaaat tctgatgatg
    14641 aggagagggc acaaggatct gatgaagata agctgcagaa ttctgacgat gatgagaaaa
    14701 tgcagaacac agatgatgag gagaggcctc agctttccga tgatgagaga caacagctat
    14761 ctgaggagga aaaggctaat tctgatgatg aacggccggt agcttctgat aatgatgatg
    14821 agaaacagaa ttctgatgat gaagaacaac cacagctgtc tgatgaagag aaaatgcaaa
    14881 attctgatga tgaaaggcca caggcctcag atgaagaaca caggcattca gatgatgaag
    14941 aggaacagga tcataaatca gaatctgcaa gaggcagtga tagtgaagat gaagttttac
    15001 gaatgaaacg caagaatgcg attgcatctg attcagaagc ggatagtgac actgaggtgc
    15061 caaaagataa tagtggaacc atggatttat ttggaggtgc agatgatatc tcttcaggga
    15121 gtgatggaga agacaaacca cctactccag gacagcctgt tgatgaaaat ggattgcctc
    15181 aggatcaaca ggaagaggag ccaattcctg agaccagaat agaagtagaa atacccaaag
    15241 taaacactga tttaggaaac gacttatatt ttgttaaact gcccaacttt ctcagtgtag
    15301 agcccagacc ttttgatcct cagtattatg aagatgaatt tgaagatgaa gaaatgctgg
    15361 atgaagaagg tagaaccagg ttaaaattaa aggtagaaaa tactataaga tggaggatac
    15421 gccgagatga agaaggaaat gaaattaaag aaagcaatgc tcggatagtc aagtggtcag
    15481 atggaagcat gtccctgcat ttaggcaatg aagtgtttga tgtgtacaaa gccccactgc
    15541 agggcgacca caatcatctt tttataagac aaggtactgg tctacaggga caagcagtct
    15601 ttaaaacgaa actcaccttc agacctcact ctacggacag tgccacacat agaaagatga
    15661 ctctgtcact tgcagatagg tgttcaaaga cacagaagat tagaatcttg ccaatggctg
    15721 gtcgtgatcc tgaatgccaa cgcacagaaa tgattaagaa agaagaagaa cgtttgaggg
    15781 cttccatacg tagggaatct cagcagcgcc gaatgagaga gaaacagcac cagcgggggc
    15841 tgagcgccag ttacctggaa cctgatcgat acgatgagga ggaggaaggc gaggagtcca
    15901 tcagcttggc tgccattaaa aaccgatata aagggggcat tcgagaggaa cgagccagaa
    15961 tctattcatc agacagtgat gagggatcag aagaagataa agctcaaaga ttactcaaag
    16021 caaagaaact taccagtgat gaggaaggtg aaccttccgg aaagagaaaa gcagaagatg
    16081 atgataaagc aaataaaaag cataagaagt atgtgatcag cgatgaagag gaagaagatg
    16141 atgattgaaa gcttgtcgag aagtactaga ggatcataat cagccatacc acatttgtag
    16201 aggttttact tgctttaaaa aacctcccac acctccccct gaacctgaaa cataaaatga
    16261 atgcaattgt tgttgttaac ttgtttattg cagcttataa tggttacaaa taaagcaata
    16321 gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca
    16381 aactcatcaa tgtatcttat catgtctgga tctgatcact gcttgagcct agaagatccg
    16441 gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta
    16501 tcataaccat ttaaataaac ggttcacata gcttagttta aatcgaccta ctccggaata
    16561 ttaatagatc atggagataa ttaaaatgat aaccatctcg caaataaata agtattttac
    16621 tgttttcgta acagttttgt aataaaaaaa cctataaata ttccggatta ttcataccgt
    16681 cccaccatcg ggcgcggatc catggcgccc accatccaga cccaggccca gcgggaggat
    16741 ggccacaggc ccaattccca ccggactctg cctgagaggt ctggagtggt ctgccgagtc
    16801 aagtactgca atagcctccc tgatatcccc ttcgacccca agttcatcac ctaccccttc
    16861 gaccagaaca ggttcgtcca gtacaaagcc acttccttgg agaaacagca caaacatgac
    16921 ctcctgact
//
