LOCUS       sequence-463641-       14154 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Protein expression in insect cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14154)
  AUTHORS   Lopez Martinez D, Todorovski I, Noe Gonzalez M, Rusimbi C, Blears D,
            Khallou N, Han Z, Dirac-Svejstrup AB, Svejstrup JQ
  TITLE     PAF1C-mediated activation of CDK12/13 kinase activity is critical 
            for CTD phosphorylation and transcript elongation.
  JOURNAL   Mol Cell. 2025 May 15;85(10):1952-1967.e8. doi: 
            10.1016/j.molcel.2025.04.012. Epub 2025 May 1.
  PUBMED    40315851
REFERENCE   2  (bases 1 to 14154)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2025.04.012"; journalName: "Mol Cell"; date: 
            "2025-05-15- 15"; volume: "85"; issue: "10"; pages: "1952-1967.e8"
COMMENT     Sequence Label: pBIG1e-CDK12deltaNTD /Cyclin K
FEATURES             Location/Qualifiers
     source          1..14154
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(60..514)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(197..218)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     408..427
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     CDS             complement(555..1346)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     mobile_element  1870..2035
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     polyA_signal    2350..2484
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        complement(4399..4490)
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     polyA_signal    4646..4780
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(7283..7306)
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             complement(7313..7336)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     promoter        complement(7382..7473)
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     polyA_signal    7629..7763
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(10266..10289)
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             complement(10296..10319)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     promoter        complement(10365..10456)
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     polyA_signal    10551..10599
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     primer_bind     10624..10643
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     protein_bind    10689..10722
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        join(11118..11123,11124..11140,11141..11146)
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     CDS             11335..11868
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     mobile_element  11935..12159
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     primer_bind     complement(12293..12310)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(12464..13052)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(12544..12563)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(13223..14014,14015..14083))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     13846..13865
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(join(14084..14154,1..34))
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccaccta
       61 aattgtaagc gttaatattt tgttaaaatt cgcgttaaat ttttgttaaa tcagctcatt
      121 ttttaaccaa taggccgaaa tcggcaaaat cccttataaa tcaaaagaat agaccgagat
      181 agggttgagt gttgttccag tttggaacaa gagtccacta ttaagaacgt ggactccaac
      241 gtcaaagggc gaaaaaccgt ctatcagggc gatggcccac tacgtgaacc atcaccctaa
      301 tcaagttttt tggggtcgag gtgccgtaaa gcactaaatc ggaaccctaa agggagcccc
      361 cgatttagag cttgacgggg aaagccggcg aacgtggcga gaaaggaagg gaagaaagcg
      421 aaaggagcgg gcgctagggc gctggcaagt gtagcggtca cgctgcgcgt aaccaccaca
      481 cccgccgcgc ttaatgcgcc gctacagggc gcgtcccatt cgccaatccg tcggcttgaa
      541 cgaattgtta gacattattt gccgactacc ttggtgatct cgcctttcac gtagtggaca
      601 aattcttcca actgatctgc gcgcgaggcc aagcgatctt cttcttgtcc aagataagcc
      661 tgtctagctt caagtatgac gggctgatac tgggccggca ggcgctccat tgcccagtcg
      721 gcagcgacat ccttcggcgc gattttgccg gttactgcgc tgtaccaaat gcgggacaac
      781 gtaagcacta catttcgctc atcgccagcc cagtcgggcg gcgagttcca tagcgttaag
      841 gtttcattta gcgcctcaaa tagatcctgt tcaggaaccg gatcaaagag ttcctccgcc
      901 gctggaccta ccaaggcaac gctatgttct cttgcttttg tcagcaagat agccagatca
      961 atgtcgatcg tggctggctc gaagatacct gcaagaatgt cattgcgctg ccattctcca
     1021 aattgcagtt cgcgcttagc tggataacgc cacggaatga tgtcgtcgtg cacaacaatg
     1081 gtgacttcta cagcgcggag aatctcgctc tctccagggg aagccgaagt ttccaaaagg
     1141 tcgttgatca aagctcgccg cgttgtttca tcaagcctta cggtcaccgt aaccagcaaa
     1201 tcaatatcac tgtgtggctt caggccgcca tccactgcgg agccgtacaa atgtacggcc
     1261 agcaacgtcg gttcgagatg gcgctcgatg acgccaacta cctctgatag ttgagtcgat
     1321 acttcggcga tcaccgcttc cctcatgatg tttaactttg ttttagggcg actgccctgc
     1381 tgcgtaacat cgttgctgct ccataacatc aaacatcgac ccacggcgta acgcgcttgc
     1441 tgcttggatg cccgaggcat agactgtacc ccaaaaaaac agtcataaca agccatgaaa
     1501 accgccactg cgccgttacc accgctgcgt tcggtcaagg ttctggacca gttgcgtgag
     1561 cgcatacgct acttaccggt ttattgacta ccggaagcag tgtgaccgtg tgcttctcaa
     1621 atgcctgagg ccagtttgct caggctctcc ccgtggaggt aataattgac gatatgatca
     1681 tttattctgc ctcccagttc aggctgcaaa taagcgttga tattcagtca attacaaaca
     1741 ttaataacga agagatgaca gaaaaatttt cattctgtga cagagaaaaa gtagccgaag
     1801 atgacggttt gtcacatgga gttggcagga tgtttgatta aaaacataac aggaagaaaa
     1861 atgccccgct gtgggcggac aaaatagttg ggaactggga ggggtggaaa tggagttttt
     1921 aaggattatt tagggaagag tgacaaaata gatgggaact gggtgtagcg tcgtaagcta
     1981 atacgaaaat taaaaatgac aaaatagttt ggaactagat ttcacttatc tggttcggat
     2041 ctcctgttta aaccaagtca atgtcagtgt ttttaattaa cccgtagtgg ctatggcagg
     2101 gcttgccgcc ccgacgttgg ctgcgagccc tgggccttca cccgaacttg ggggttgggg
     2161 tggggaaaag gaagaaacgc gggcgtattg gtcccaatgg ggtctcggtg gggtatcgac
     2221 gtaaccccga ttgagatata gatttattta aatggttatg atagttattg ctcagcggtg
     2281 gcagcagcca actcagcttc ctttcgggct ttgttagcag ccggatcttc taggctcaag
     2341 cagtgatcag atccagacat gataagatac attgatgagt ttggacaaac cacaactaga
     2401 atgcagtgaa aaaaatgctt tatttgtgaa atttgtgatg ctattgcttt atttgtaacc
     2461 attataagct gcaataaaca agttaacaac aacaattgca ttcattttat gtttcaggtt
     2521 cagggggagg tgtgggaggt tttttaaagc aagtaaaacc tctacaaatg tggtatggct
     2581 gattatgatc ctctagtact tctcgacaag cttttatctc atccaggctg cccgccccag
     2641 ccccccaact ggaggcatgc cgggtggggg aatgggcggg ggcacaggag gctgtcctcc
     2701 aggggggacg gcaggaggtg ggtagctggc tggcggcagg cccaaccctg gaggaggatg
     2761 aggggggact gcgtgggtag gcgggaggcg tgggggtggg ggtgggaagt tggggttgta
     2821 ggtgggtggg ggtggggggt agccaggagt agggggtggg atggcaggtg gggggaagga
     2881 ggctggggga ggaggcacag gtggcggggg cgggttgggc gggtagacat gggggtggta
     2941 gggcaggtgt gcaggtgggc cgtaggcggg gggcagggca ccgtaggagg gtccctcggt
     3001 cttcatcatg gactgcaggc tctggtagcc ctctccagac atgtaggagc tggtggtgga
     3061 catcccggtc atgtagctgg aggggggtgg gggcgggggc cggtgtggca gcggcggtgg
     3121 ctggtgcaca ggggccgggg gggccggagg gggaatctgg actttgggga ggtcagtggc
     3181 atccacaggg cccggcggct cagcctcacc taaggcagca gcgaggggag gcttccggtc
     3241 tggaggtggg tgggctggag gcaacggtgg atgagtggtc tcaattttgg ggattttagg
     3301 tggtggtggt tctgctgctt tgttctcttc tttgggagaa acaaccacgg ctcgcttaac
     3361 ctgtcgggga gaactgggct gcggagaggg tttcttgggc tgttgggctg gctgctgctg
     3421 ctgggctggt tgctgggggt ccttctgctg gggctgggat ggttcggagc tttgagacgg
     3481 ctgtgactgc tgtacttgcg gcacttgtgg tgtaggctga agagatgggg gctgttgcag
     3541 ctgatggggg gtgtgatgag gcatctgttg ttttccttgt gagtaaagat ccaggatttg
     3601 gtggcagatg tcttccaaaa cgtcgaccgg gacatcttga acaaactgct cccaccatct
     3661 cctatacatg ggtttggagg tccattcttg tatttcaaat ttgcacaaac gtcctgcgag
     3721 atacatcact gctactgcta tgatctctgg ttcccactgc agtgacaagg tggtgcagag
     3781 actgtcattt acaaatgtcc atgccatttg aaccaacttt tgaattttgt ttttatcacc
     3841 tttgagttgc tttgcatatt ttagtaggaa ctggtatgga tgttctacct gtaaatcaaa
     3901 cttgatggtc tgcagtaaga ttctctccag aaccattact tcctcctttg ggtcatctcc
     3961 aaactggcca aattgtacat catttaataa actacgagct gttttgatga tatctttaca
     4021 tttttttggt gtttcttcta ctttcccagc cagaaagagg caacaggctc ctgtcacata
     4081 tcttgggaat tgcttgaagg aatgaaacat atagaagcga tgaaaataaa ttattccagt
     4141 tgccagggta tcatagtgta gccccaaacg tgtgcccaca tcaaagatga accgagcgcc
     4201 ctctcggcgg taccgggcct cggtggctgg atcaagtcct tcaagttgtg agggtgtatg
     4261 agccaagtct ttcttatccc agtaccaaca tggctttgtg tggtccaggt ttgctgaagt
     4321 tactgaaggg cttgaatttt ctttattctc cttcatggat ccgcgcccga tggtgggacg
     4381 gtatgaataa tccggaatat ttataggttt ttttattaca aaactgttac gaaaacagta
     4441 aaatacttat ttatttgcga gatggttatc attttaatta tctccatgat ctattaatat
     4501 tccggagtag gtcgatttaa acgtgcaata gtatccagtt tatttaaatg gttatgatag
     4561 ttattgctca gcggtggcag cagccaactc agcttccttt cgggctttgt tagcagccgg
     4621 atcttctagg ctcaagcagt gatcagatcc agacatgata agatacattg atgagtttgg
     4681 acaaaccaca actagaatgc agtgaaaaaa atgctttatt tgtgaaattt gtgatgctat
     4741 tgctttattt gtaaccatta taagctgcaa taaacaagtt aacaacaaca attgcattca
     4801 ttttatgttt caggttcagg gggaggtgtg ggaggttttt taaagcaagt aaaacctcta
     4861 caaatgtggt atggctgatt atgatcctct agtacttctc gacaagcttt tagtaaggaa
     4921 ctcctctccc tcttccccct cttggtggga ctcttgtagg cccccgatag agttttccat
     4981 aagcagaaga ctgagttggg cccccccagt gaaggcctgc tcctgagctg ctggccccag
     5041 tggttcctgg ccccagctcc ccatagtttt gcaatttggt ctctgcatgt accacagaat
     5101 tgctgcttcc ctcagccttc aggaatggtt gcccgaccac cgggtgtaac gctaagggga
     5161 ccctggcaaa acggaggtcc tggtcccctg gaaactgcac tgaccctgtg ccctggtaac
     5221 tgctggactc cccaaggtgg ctcagagagc ctgagaaggt cctgttcttc accagggtct
     5281 ggaccaagga ttctgtcaag gctggaccag agtttcgttc atcagtgtca atggcactga
     5341 accctgtttc aggcccatca gtgtttccat aagtcctgtt tggacggggt cgggtggagt
     5401 actccattgg tctcaaggcc tggtgctcat gtggcaggtg gccaggaggc tctgcttcag
     5461 gctgggataa aagttgcagc aaggcggctg tcacggctgg gttcaactcc tggggggcgc
     5521 tggaaagatc gccttcaacc agagggggtg gaggtggcgg cggtggaggt ccggggggct
     5581 cagggggcct cttctctggt ggaagaatgt gaggaggaca tgctgctgcc tcctcctgtg
     5641 gcattgtggg agttcttcgg ggcccctgtg gcccactgtt cttgtcactg ttgttttcct
     5701 ccagactgcc tgctggctct tgggttttca tcagctgact caagagaact gccaatatat
     5761 tctgcatgtc agctggtgtg cttgaagctt caagggtcgt ctgttctgct gaaggcagga
     5821 tcactggggc agagggtgct tccttcaaag actcctctgg ggccatggtc tctgagtcct
     5881 gctgctggga agtagcttcc gtcagggcac tgatggattg gttcagggct tccagctgct
     5941 gctgcatctc tgggttggag tggatgttaa gcagctgtgc catttgaggg atgctcaggt
     6001 cggtttggct ctgcagcagg tttaataaca ctgccaattc actttgattc agctgttgtg
     6061 tgatgtcagc aaggcctatt gcatccccag ccccagactc caccttgcca ggagcaggct
     6121 gaggtggtgc tgggctgctg ttcttcacag gctcagtact tgtccctgag gtagtttctt
     6181 ttcgagaagt tttggatgga ggtggctctt cgactacaac accactttgt cgctgacgtc
     6241 gccgtttctt actccacaac tcatggcaat cctgccagtg ggggaggtct ggaggagcca
     6301 ttttgctgag ttcgacatct ttaaggaagt cgctctgtag ggtctgttca gctgtgcacc
     6361 gcttactagg atctagtgtc agcatgtggt ccaataaatc aagtgctgca gaaggaatga
     6421 aagagaattc ttctcgtaga cgccttcgat attgcttctt cggtttcatg gtgttgaagt
     6481 agggcagttt gataacatca ggccacacag ctggacaagg gctaccacaa agtcggctga
     6541 tcagttctag ctgagccagt tccagattgg cttgaaaaat aggcttcttt gtgaatagtt
     6601 ccccaagaat acatccacag ctccaaacat ctatggctgg tgtgtaacgt tcctctccta
     6661 gcagtagttc tggaggtcgg taccacaaag taatgacttt gtttgtgtaa gggcgactct
     6721 cttcagagtt atagagccga gcaagtccaa aatctgctag tttgatttgc ccactgttat
     6781 tcagcaaaat gttagaacac ttaatatccc gatgcaggaa attctttttg tgacagtatt
     6841 ccaatccttc cattagctgt ttcatgaacg acttgatatg gtcctcagaa aagtgcacca
     6901 aaccagattc tagcagtccc attaagtcat ggtccatata ctcaaataca aggtaaaagg
     6961 cacctttgtc cttcttgaaa tccagtgcat cttgtttatc tgtgacaatt tccttcatgt
     7021 taacaacact tcggtggatt aactgacgaa ggattttgat ttcacgaatg gctgtgattg
     7081 ggaagccctc tttctcattg tctagtctca ccttcttcag agccactagt tctcctgtgt
     7141 ctttgtcctt ggctttatat acttggccat aggttccttc tccaataatc ccaataatgt
     7201 caaacttgtc cacacagcgt ttcccccagt cgctttctgt ttgtcttctt tctccataac
     7261 gaggacaaca aatttttggc atgggtccct gaaagaggac ttcaagatcg agcttgtcat
     7321 cgtcgtcctt gtagtccatg gatccgcgcc cgatggtggg acggtatgaa taatccggaa
     7381 tatttatagg tttttttatt acaaaactgt tacgaaaaca gtaaaatact tatttatttg
     7441 cgagatggtt atcattttaa ttatctccat gatctattaa tattccggag taggtcgatt
     7501 taaacgtgca atagtatcca gtttatttaa atggttatga tagttattgc tcagcggtgg
     7561 cagcagccaa ctcagcttcc tttcgggctt tgttagcagc cggatcttct aggctcaagc
     7621 agtgatcaga tccagacatg ataagataca ttgatgagtt tggacaaacc acaactagaa
     7681 tgcagtgaaa aaaatgcttt atttgtgaaa tttgtgatgc tattgcttta tttgtaacca
     7741 ttataagctg caataaacaa gttaacaaca acaattgcat tcattttatg tttcaggttc
     7801 agggggaggt gtgggaggtt ttttaaagca agtaaaacct ctacaaatgt ggtatggctg
     7861 attatgatcc tctagtactt ctcgacaagc ttttagtaag gaactcctct ccctcttccc
     7921 cctcttggtg ggactcttgt aggcccccga tagagttttc cataagcaga agactgagtt
     7981 gggccccccc agtgaaggcc tgctcctgag ctgctggccc cagtggttcc tggccccagc
     8041 tccccatagt tttgcaattt ggtctctgca tgtaccacag aattgctgct tccctcagcc
     8101 ttcaggaatg gttgcccgac caccgggtgt aacgctaagg ggaccctggc aaaacggagg
     8161 tcctggtccc ctggaaactg cactgaccct gtgccctggt aactgctgga ctccccaagg
     8221 tggctcagag agcctgagaa ggtcctgttc ttcaccaggg tctggaccaa ggattctgtc
     8281 aaggctggac cagagtttcg ttcatcagtg tcaatggcac tgaaccctgt ttcaggccca
     8341 tcagtgtttc cataagtcct gtttggacgg ggtcgggtgg agtactccat tggtctcaag
     8401 gcctggtgct catgtggcag gtggccagga ggctctgctt caggctggga taaaagttgc
     8461 agcaaggcgg ctgtcacggc tgggttcaac tcctgggggg cgctggaaag atcgccttca
     8521 accagagggg gtggaggtgg cggcggtgga ggtccggggg gctcaggggg cctcttctct
     8581 ggtggaagaa tgtgaggagg acatgctgct gcctcctcct gtggcattgt gggagttctt
     8641 cggggcccct gtggcccact gttcttgtca ctgttgtttt cctccagact gcctgctggc
     8701 tcttgggttt tcatcagctg actcaagaga actgccaata tattctgcat gtcagctggt
     8761 gtgcttgaag cttcaagggt cgtctgttct gctgaaggca ggatcactgg ggcagagggt
     8821 gcttccttca aagactcctc tggggccatg gtctctgagt cctgctgctg ggaagtagct
     8881 tccgtcaggg cactgatgga ttggttcagg gcttccagct gctgctgcat ctctgggttg
     8941 gagtggatgt taagcagctg tgccatttga gggatgctca ggtcggtttg gctctgcagc
     9001 aggtttaata acactgccaa ttcactttga ttcagctgtt gtgtgatgtc agcaaggcct
     9061 attgcatccc cagccccaga ctccaccttg ccaggagcag gctgaggtgg tgctgggctg
     9121 ctgttcttca caggctcagt acttgtccct gaggtagttt cttttcgaga agttttggat
     9181 ggaggtggct cttcgactac aacaccactt tgtcgctgac gtcgccgttt cttactccac
     9241 aactcatggc aatcctgcca gtgggggagg tctggaggag ccattttgct gagttcgaca
     9301 tctttaagga agtcgctctg tagggtctgt tcagctgtgc accgcttact aggatctagt
     9361 gtcagcatgt ggtccaataa atcaagtgct gcagaaggaa tgaaagagaa ttcttctcgt
     9421 agacgccttc gatattgctt cttcggtttc atggtgttga agtagggcag tttgataaca
     9481 tcaggccaca cagctggaca agggctacca caaagtcggc tgatcagttc tagctgagcc
     9541 agttccagat tggcttgaaa aataggcttc tttgtgaata gttccccaag aatacatcca
     9601 cagctccaaa catctatggc tggtgtgtaa cgttcctctc ctagcagtag ttctggaggt
     9661 cggtaccaca aagtaatgac tttgtttgtg taagggcgac tctcttcaga gttatagagc
     9721 cgagcaagtc caaaatctgc tagtttgatt tgcccactgt tattcagcaa aatgttagaa
     9781 cacttaatat cccgatgcag gaaattcttt ttgtgacagt attccaatcc ttccattagc
     9841 tgtttcatga acgacttgat atggtcctca gaaaagtgca ccaaaccaga ttctagcagt
     9901 cccattaagt catggtccat atactcaaat acaaggtaaa aggcaccttt gtccttcttg
     9961 aaatccagtg catcttgttt atctgtgaca atttccttca tgttaacaac acttcggtgg
    10021 attaactgac gaaggatttt gatttcacga atggctgtga ttgggaagcc ctctttctca
    10081 ttgtctagtc tcaccttctt cagagccact agttctcctg tgtctttgtc cttggcttta
    10141 tatacttggc cataggttcc ttctccaata atcccaataa tgtcaaactt gtccacacag
    10201 cgtttccccc agtcgctttc tgtttgtctt ctttctccat aacgaggaca acaaattttt
    10261 ggcatgggtc cctgaaagag gacttcaaga tcgagcttgt catcgtcgtc cttgtagtcc
    10321 atggatccgc gcccgatggt gggacggtat gaataatccg gaatatttat aggttttttt
    10381 attacaaaac tgttacgaaa acagtaaaat acttatttat ttgcgagatg gttatcattt
    10441 taattatctc catgatctat taatattccg gagtaggtcg atttaaatct ttagaccata
    10501 gagcgttcac ttgacagagt gccagccctg ggaccgaacc ccgcgtttat gaacaaacga
    10561 cccaacaccc gtgcgtttta ttctgtcttt ttattgccgt catagcgcgg gttccttccg
    10621 gtattgtctc cttccgtgtt tcagttagcc tcgataaaaa ctaagctatg tgaaccgttt
    10681 aaacccagat aacttcgtat aatgtatgct atacgaagtt atggtaccgc ggccgcgtag
    10741 aggatctgtt gatcagcagt tcaacctgtt gatagtacaa acaaagctgt agccaaccac
    10801 tagaactata gctagagtcc tgggcgaaca aacgatgctc gccttccaga aaaccgagga
    10861 tgcgaaccac ttcatccggg gtcagcacca ccggcaagcg ccgcgacggc cgaggtctac
    10921 cgatctcctg aagcaagggc agatccgtgc acagcacctt gccgtagaag aacagcaagg
    10981 ccgccaatgc ctgacgatgc gtggagaccg aaaccttgcg ctcgttcgcc agccaggaca
    11041 gaaatgcctc gacttcgctg ctgcccaagg ttgccgggtg acgcacaccg tggaaacgga
    11101 tgaaggcaca aacccagttg acataagcct gttcggttcg taaactgtaa tgcaagtagc
    11161 gtatgcgctc acgcaactgg tccagaacct tgaccgaacg cagcggtggt aacggcgcag
    11221 tggcggtttt catggcttgt tatgactgtt tttttgtaca gtctatgcct cgggcatcca
    11281 agcagcaagc gcgttacgcc gtgggtcgat gtttgatgtt atggagcagc aacgatgtta
    11341 cgcagcagca acgatgttac gcagcagggc agtcgcccta aaacaaagtt aggtggctca
    11401 agtatgggca tcattcgcac atgtaggctc ggccctgacc aagtcaaatc catgcgggct
    11461 gctcttgatc ttttcggtcg tgagttcgga gacgtagcca cctactccca acatcagccg
    11521 gactccgatt acctcgggaa cttgctccgt agtaagacat tcatcgcgct tgctgccttc
    11581 gaccaagaag cggttgttgg cgctctcgcg gcttacgttc tgcccaagtt tgagcagccg
    11641 cgtagtgaga tctatatcta tgatctcgca gtctccggcg agcaccggag gcagggcatt
    11701 gccaccgcgc tcatcaatct cctcaagcat gaggccaacg cgcttggtgc ttatgtgatc
    11761 tacgtgcaag cagattacgg tgacgatccc gcagtggctc tctatacaaa gttgggcata
    11821 cgggaagaag tgatgcactt tgatatcgac ccaagtaccg ccacctaaca attcgttcaa
    11881 gccgagatcg gcttcccggc cgcggagttg ttcggtaaat tgtcacaacg ccgcgaatat
    11941 agtctttacc atgcccttgg ccacgcccct ctttaatacg acgggcaatt tgcacttcag
    12001 aaaatgaaga gtttgcttta gccataacaa aagtccagta tgctttttca cagcataact
    12061 ggactgattt cagtttacaa ctattctgtc tagtttaaga ctttattgtc atagtttaga
    12121 tctattttgt tcagtttaag actttattgt ccgcccacac ccgcttacgc agggcatcca
    12181 tttattactc aaccgtaacc gattttgcca ggttacgcgg ctggtctatg cggtgtgaaa
    12241 taccgcacag atgcgtaagg agaaaatacc gcatcaggcg ctcttccgct tcctcgctca
    12301 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg
    12361 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc
    12421 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc
    12481 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac
    12541 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc
    12601 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata
    12661 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc
    12721 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
    12781 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag
    12841 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta
    12901 gaagaatagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg
    12961 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc
    13021 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt
    13081 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa
    13141 ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat
    13201 atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga
    13261 tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac
    13321 gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg
    13381 ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg
    13441 caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt
    13501 cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct
    13561 cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat
    13621 cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta
    13681 agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca
    13741 tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat
    13801 agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac
    13861 atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa
    13921 ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt
    13981 cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg
    14041 caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat
    14101 attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaat
//
