LOCUS       sequence-463644-       10185 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  EIF3D overexpression vector with non-targeting sgRNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10185)
  AUTHORS   She R, Luo J, Weissman JS
  TITLE     Translational fidelity screens in mammalian cells reveal eIF3 and 
            eIF4G2 as regulators of start codon selectivity.
  JOURNAL   Nucleic Acids Res. 2023 Jul 7;51(12):6355-6369. doi: 
            10.1093/nar/gkad329.
  PUBMED    37144468
REFERENCE   2  (bases 1 to 10185)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nucleic Acids Res"; date: "2023-07-7- 7"; volume: "51"; issue: 
            "12"; pages: "6355-6369"
COMMENT     Sequence Label: pEIF3D-WT-sgNT
FEATURES             Location/Qualifiers
     source          1..10185
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(242..259)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    248..472
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     misc_feature    531..648
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(946..990)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1175..1408
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    1905..2030
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             2077..2257
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(2275..2473)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(2300..2320)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        2475..2854
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     3026..3045
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        3120..3224
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3225..3293,3294..4085)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3443..3462)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4256..4844
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4745..4764
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     LTR             4906..5086
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     5595..5611
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     5596..5612
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    5615..6203
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             5721..5732
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     6130..6150
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     protein_bind    6259..6292
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    complement(6506..6539)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     6543..6563
                     /label=mU6-F
                     /note="Mouse U6 promoter, forward primer"
     promoter        6887..7294
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     CDS             7306..7902
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(7306..7325)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     7802..7822
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     CDS             7912..7965
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             7978..8673
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="SELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKVV
                     EGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTAT
                     QDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALKL
                     VGGSHLIANIKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYCD
                     LPSKLGHKLN"
     CDS             8683..8739
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
ORIGIN
        1 gtggagaatg cctggggcat tttacgctgc gtcattgaca tctgcatgaa gctggaggag
       61 ggcaaatacc tcatcctcaa ggaccccaac aagcaggtca tccgtgtcta cagcctccct
      121 gatggcacct tcagctctga tgaagatgag gaggaagagg aggaggaaga agaggaagaa
      181 gaagaggaag aaacttaaga attcctaggg cctagagggc ccgtttaaac ccgctgatca
      241 gcctcgactg tgccttctag ttgccagcca tctgttgttt gcccctcccc cgtgccttcc
      301 ttgaccctgg aaggtgccac tcccactgtc ctttcctaat aaaatgagga aattgcatcg
      361 cattgtctga gtaggtgtca ttctattctg gggggtgggg tggggcagga cagcaagggg
      421 gaggattggg aagacaatag caggcatgct ggggatgcgg tgggctctat ggtctagagc
      481 gggcccggta ctaaccaaac tggatctctg ctgtccctgt aataaacccg aaaattttga
      541 atttttgtaa tttgtttttg taattcttta gtttgtatgt ctgttgctat tatgtctact
      601 attctttccc ctgcactgta ccccccaatc cccccttttc ttttaaaatt gtggatgaat
      661 actgccattt gtctgcagaa ttggcgcacg cagtgccgat ccgttcacta atcgaatgga
      721 tctgtctctg tctctctctc caccttcttc ttctattcct tcgggcctgt cgggtcccct
      781 cggggttggg aggtgggtct gaaacgataa tggtgaatat ccctgcctaa ctctattcac
      841 tatagaaagt acagcaaaaa ctattcttaa acctaccaag cctcctacta tcattatgaa
      901 taattttata taccacagcc aatttgttat gttaaaccaa ttccacaaac ttgcccattt
      961 atctaattcc aataattctt gttcattctt ttcttgctgg ttttgcgatt cttcaattaa
     1021 ggagtgtatt aagcttgtgt aattgttaat ttctctgtcc cactccatcc aggtcgtgtg
     1081 attccaaatc tgttccagag atttattact ccaactagca ttccaaggca cagcagtggt
     1141 gcaaatgagt tttccagagc aaccccaaat ccccaggagc tgttgatcct ttaggtatct
     1201 ttccacagcc aggattcttg cctggagctg cttgatgccc cagactgtga gttgcaacag
     1261 atgctgttgc gcctcaatag ccctcagcaa attgttctgc tgctgcacta taccagacaa
     1321 taattgtctg gcctgtaccg tcagcgtcat tgacgctgcg cccatagtgc ttcctgctgc
     1381 tcccaagaac ccaaggaaca aagctcctat tcccactgct cttttttctc tctgcaccac
     1441 tcttctcttt gccttggtgg gtgctactcc taatggttca atttttacta ctttatattt
     1501 atataattca cttctccaat tgtccctcat atctcctcct ccaggtctga agatcagcgg
     1561 ccggccgctt gctgtgcggt ggtcttactt ttgttttgct cttcctctat cttgtctaaa
     1621 gcttccttgg tgtcttttat ctctatcctt tgatgcacac aatagagggt tgctactgta
     1681 ttatataatg atctaagttc ttctgatcct gtctgaaggg atggttgtag ctgtcccagt
     1741 atttgtctac agccttctga tgtttctaac aggccaggat taactgcgaa tcgttctagc
     1801 tccctgcttg cccatactat atgttttaat ttatattttt tctttccccc tggccttaac
     1861 cgaatttttt cccatcgcga tctaattctc ccccgcttaa tactgacgct ctcgcaccca
     1921 tctctctcct tctagcctcc gctagtcaaa atttttggcg tactcaccag tcgccgcccc
     1981 tcgcctcttg ccgtgcgcgc ttcagcaagc cgagtcctgc gtcgagagag ctcctctggt
     2041 ttccctttcg ctttcaagtc cctgttcggg cgccactgct agagattttc cacactgact
     2101 aaaagggtct gagggatctc tagttaccag agtcacacaa cagacgggca cacactactt
     2161 gaagcactca aggcaagctt tattgaggct taagcagtgg gttccctagt tagccagaga
     2221 gctcccaggc tcagatctgg tctaaccaga gagacccagt acaggcaaaa cgcgctgctt
     2281 atatagacct cccaccgtac acgcctaccg cccatttgcg tcaatggggc ggagttgtta
     2341 cgacattttg gaaagtcccg ttgattttgg tgccaaaaca aactcccatt gacgtcaatg
     2401 gggtggagac ttggaaatcc ccgtgagtca aaccgctatc cacgcccatt gatgtactgc
     2461 caaaaccgca tcaccatggt aatagcgatg actaatacgt agatgtactg ccaagtagga
     2521 aagtcccata aggtcatgta ctgggcataa tgccaggcgg gccatttacc gtcattgacg
     2581 tcaatagggg gcgtacttgg catatgatac acttgatgta ctgccaagtg ggcagtttac
     2641 cgtaaatact ccacccattg acgtcaatgg aaagtcccta ttggcgttac tatgggaaca
     2701 tacgtcatta ttgacgtcaa tgggcggggg tcgttgggcg gtcagccagg cgggccattt
     2761 accgtaagtt atgtaacgcg gaactccata tatgggctat gaactaatga ccccgtaatt
     2821 gattactatt aataactagt caataatcaa tgtcaacgcg tatatctggc ccgtacatcg
     2881 cgaagcagcg caaaacgcct aaccctaagc agattcttca tgcaattgtc ggtcaagcct
     2941 tgccttgttg tagcttaaat tttgctcgcg cactactcag cgacctccaa cacacaagca
     3001 gggagcagat actggcttaa ctatgcggca tcagagcaga ttgtactgag agtgcaccat
     3061 aggggatcgg gagatctccc gatccgtcga cgtcaggtgg cacttttcgg ggaaatgtgc
     3121 gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac
     3181 aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt
     3241 tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag
     3301 aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg
     3361 aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa
     3421 tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc
     3481 aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag
     3541 tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa
     3601 ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc
     3661 taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg
     3721 agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa
     3781 caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa
     3841 tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg
     3901 gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag
     3961 cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg
     4021 caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt
     4081 ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt
     4141 aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac
     4201 gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag
     4261 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg
     4321 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca
     4381 gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga
     4441 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca
     4501 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc
     4561 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca
     4621 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa
     4681 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc
     4741 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc
     4801 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg
     4861 cctttttacg gttcctggcc ttttgctggc cttttgctca catgctgcta gagattttcc
     4921 acactgacta aaagggtctg agggatctct agttaccaga gtcacacaac agacgggcac
     4981 acactacttg aagcactcaa ggcaagcttt attgaggctt aagcagtggg ttccctagtt
     5041 agccagagag ctcccaggct cagatctggt ctaaccagag agacccagta cagtccggat
     5101 gcagctctcg ggccatgtga tgaaatgcta ggcggctgtc aaacctccac tctaatactt
     5161 ctctctccgg gtcatccatc ccatgcaggc tcacagggtg taacaagcgg gtgttctctc
     5221 cttcattggc ttcttctacc ttctcttgct caactggtac tagcttgtag caccatccaa
     5281 aggtcagtgg atatctgatc cctggccctg gtgtgtagtt ctgccaatca gggaagtagc
     5341 cttgtgtgtg gtagatccac agatcaagga tatcttgtct tcgttgggag tgaattagcc
     5401 cttccagtcc ccccttttct tttaaaaagt ggctaagatc tacagctgcc ttgtaagtca
     5461 ttggtcttaa aggtacctga ggtgtgactg gaaaacccac ctcctcctcc tcttgtgctt
     5521 ctagccaggc acaatcagca ttggtagctg ctgtattgct acttgtgatt gctccatgtt
     5581 tttctaggtc tcgatcgagg tcgacggtat cgatgcgggg aggcggccca aagggagatc
     5641 cgactcgtct gagggcgaag gcgaagacgc ggaagaggcc gcagagccgg cagcaggccg
     5701 cgggaaggaa ggtccgctgg attgagggcc gaagggacgt agcagaagga cgtcccgcgc
     5761 agaatccagg tggcaacaca ggcgagcagc caaggaaagg acgatgattt ccccgacaac
     5821 accacggaat tgtcagtgcc caacagccga gcccctgtcc agcagcgggc aaggcaggcg
     5881 gcgatgagtt ccgccgtggc aatagggagg gggaaagcga aagtcccgga aaggagctga
     5941 caggtggtgg caatgcccca accagtgggg gttgcgtcag caaacacagt gcacaccacg
     6001 ccacgttgcc tgacaacggg ccacaactcc tcataaagag acagcaacca ggatttatac
     6061 aaggaggaga aaatgaaagc catacgggaa gcaatagcat gatacaaagg cattaaagca
     6121 gcgtatccac atagcgtaaa aggagcaaca tagttaagaa taccagtcaa tctttcacaa
     6181 attttgtaat ccagaggttg attatcgata agcttgatat cgaattgtac ctagtggaac
     6241 cggaaccctt aaacatgtat aacttcgtat aatgtatgct atacgaagtt attaggtccc
     6301 tcgacgttaa cgatccgacg cgccatctct aggcccgcgc cggccccctc gcacggactt
     6361 gtgggagaag ctcggctact cccctgcccc ggttaatttg catataatat ttcctagtaa
     6421 ctatagaggc ttaatgtgcg ataaaagaca gataatctgt tctttttaat actagctaca
     6481 ttttacatga taggcttgga tttctataac ttcgtatagc atacattata cgaagttata
     6541 aacagcacaa aaggaaactc accctaactg taaagtaatt gtgtgttttg agactataag
     6601 tatcccttgg agaaccacct tgttggacca ggatgggcac cacccgttta agagctaagc
     6661 tggaaacagc atagcaagtt taaataaggc tagtccgtta tcaacttgaa aaagtggcac
     6721 cgagtcggtg ctttttttct cgagtactag gatccattag gcggccgcgt ggataaccgt
     6781 attaccgcca tgcattgcag ccccgataaa ataaaagatt ttatttagtc tccagaaaaa
     6841 ggggggaatg aaagacccca cctgtaggtt tggcaagcta gctgcagtaa cgccattttg
     6901 caaggcatgg aaaaatacca aaccaagaat agagaagttc agatcaaggg cgggtacatg
     6961 aaaatagcta acgttgggcc aaacaggata tctgcggtga gcagtttcag ccccggcccg
     7021 gggccaagaa cagatggtca ccgcagtttc ggccccggcc cgaggccaag aacagatggt
     7081 ccccagatat ggcccaaccc tcagcagttt cttaagaccc atcagatgtt tccaggctcc
     7141 cccaaggacc tgaaatgacc ctgcgcctta tttgaattaa ccaatcagcc tgcttctcgc
     7201 ttctgttcgc gcgcttctgc ttcccgagct ctataaaaga gctcacaacc cctcactcgg
     7261 cgcgccagtc ctccgacaga ctgagtcgcc cggggctagc ccaccatgac cgagtacaag
     7321 cccacggtgc gcctcgccac ccgcgacgac gtcccccggg ccgtacgcac cctcgccgcc
     7381 gcgttcgccg actaccccgc cacgcgccac accgtcgacc cggaccgcca catcgagcgg
     7441 gtcaccgagc tgcaagaact cttcctcacg cgcgtcgggc tcgacatcgg caaggtgtgg
     7501 gtcgcggacg acggcgccgc ggtggcggtc tggaccacgc cggagagcgt cgaagcgggg
     7561 gcggtgttcg ccgagatcgg cccgcgcatg gccgagttga gcggttcccg gctggccgcg
     7621 cagcaacaga tggaaggcct cctggcgccg caccggccca aggagcccgc gtggttcctg
     7681 gccaccgtcg gcgtctcgcc cgaccaccag ggcaagggtc tgggcagcgc cgtcgtgctc
     7741 cccggagtgg aggcggccga gcgcgccggg gtgcccgcct tcctggagac ctccgcgccc
     7801 cgcaacctcc ccttctacga gcggctcggc ttcaccgtca ccgccgacgt cgaggtgccc
     7861 gaaggaccgc gcacctggtg catgacccgc aagcccggtg ccggatcggg agagggcaga
     7921 ggaagtctgc taacatgcgg tgacgtcgag gagaatcctg gcccaccggt cgccaccagc
     7981 gagctgatta aggagaacat gcacatgaag ctgtacatgg agggcaccgt ggacaaccat
     8041 cacttcaagt gcacatccga gggcgaaggc aagccctacg agggcaccca gaccatgaga
     8101 atcaaggtgg tcgagggcgg ccctctcccc ttcgccttcg acatcctggc tactagcttc
     8161 ctctacggca gcaagacctt catcaaccac acccagggca tccccgactt cttcaagcag
     8221 tccttccctg agggcttcac atgggagaga gtcaccacat acgaagacgg gggcgtgctg
     8281 accgctaccc aggacaccag cctccaggac ggctgcctca tctacaacgt caagatcaga
     8341 ggggtgaact tcacatccaa cggccctgtg atgcagaaga aaacactcgg ctgggaggcc
     8401 ttcaccgaga cgctgtaccc cgctgacggc ggcctggaag gcagaaacga catggccctg
     8461 aagctcgtgg gcgggagcca tctgatcgca aacatcaaga ccacatatag atccaagaaa
     8521 cccgctaaga acctcaagat gcctggcgtc tactatgtgg actacagact ggaaagaatc
     8581 aaggaggcca acaacgagac ctacgtcgag cagcacgagg tggcagtggc cagatactgc
     8641 gacctcccta gcaaactggg gcacaagctt aatggtagtg gggctactaa cttcagcctg
     8701 ctgaagcagg ctggagacgt ggaggagaac cctggacctg caaagttcat gacacccgtg
     8761 atccaggaca acccctcagg ctggggtccc tgtgcggttc ccgagcagtt tcgggatatg
     8821 ccctaccagc cgttcagcaa aggagatcgg ctaggaaagg ttgcagactg gacaggagcc
     8881 acataccaag ataagaggta cacaaataag tactcctctc agtttggtgg tggaagtcaa
     8941 tatgcttatt tccatgagga ggatgaaagt agcttccagc tggtggatac agcgcgcaca
     9001 cagaagacgg cctaccagcg gaatcgaatg agatttgccc agaggaacct ccgcagagac
     9061 aaagatcgtc ggaacatgtt gcagttcaac ctgcagatcc tgcctaagag tgccaaacag
     9121 aaagagagag aacgcattcg actgcagaaa aagttccaga aacaatttgg ggttaggcag
     9181 aaatgggatc agaaatcaca gaaaccccga gactcttcag ttgaagttcg tagtgattgg
     9241 gaagtgaaag aggaaatgga ttttcctcag ttgatgaaga tgcgctactt ggaagtatca
     9301 gagccacagg acattgagtg ttgtggggcc ctagaatact acgacaaagc ctttgaccgc
     9361 atcaccacga ggagtgagaa gccactgcgg agcatcaagc gcatcttcca cactgtcacc
     9421 accacagacg accctgtcat ccgcaagctg gcaaaaactc aggggaatgt gtttgccact
     9481 gatgccatcc tggccacgct gatgagctgt acccgctcag tgtattcctg ggatattgtc
     9541 gtccagagag ttgggtccaa actcttcttt gacaagagag acaactctga ctttgacctc
     9601 ctgacagtga gtgagactgc caatgagccc cctcaagatg aaggtaattc cttcaattca
     9661 ccccgcaacc tggccatgga ggcaacctac atcaaccaca atttctccca gcagtgcttg
     9721 agaatgggga aggaaagata caacttcccc aacccaaacc cgtttgtgga ggacgacatg
     9781 gataagaatg aaatcgcctc tgttgcgtac cgttaccgca ggtggaagct tggagatgat
     9841 attgacctta ttgtccgttg tgagcacgat ggcgtcatga ctggagccaa cggggaagtg
     9901 tccttcatca acatcaagac actcaatgag tgggattcca ggcactgtaa tggcgttgac
     9961 tggcgtcaga agctggactc tcagcgaggg gctgtcattg ccacggagct gaagaacaac
    10021 agctacaagt tggcccggtg gacctgctgt gctttgctgg ctggatctga gtacctcaag
    10081 cttggttatg tgtctcggta ccacgtgaaa gactcctcac gccacgtcat cctaggcacc
    10141 cagcagttca agcctaatga gtttgccagc cagatcaacc tgagc
//
