LOCUS       sequence-46369-e         619 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 619)
  AUTHORS   Najdi R, Proffitt K, Sprowl S, Kaur S, Yu J, Covey TM, Virshup DM, 
            Waterman ML
  TITLE     A uniform human Wnt expression library reveals a shared secretory 
            pathway and unique signaling activities.
  JOURNAL   Differentiation. 2012 Sep;84(2):203-13. doi: 
            10.1016/j.diff.2012.06.004. Epub 2012 Jul 9.
  PUBMED    22784633
REFERENCE   2  (bases 1 to 619)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.diff.2012.06.004"; journalName: "Differentiation"; date: 
            "2012-09"; volume: "84"; issue: "2"; pages: "203-13"
COMMENT     Sequence Label: pcDNA-Wnt3A-V5
FEATURES             Location/Qualifiers
     source          1..619
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(9..50)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    76..100
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 ccggtacgcg tagaatcgag accgaggaga gggttaggga taggcttacc ttcgaaccgc
       61 gggccctcta gatcaaccac tttgtacaag aaagctgggt cggcgcgccc acccttcaga
      121 atgcacttgc aggtgtgcac gtcgtagacg cgcgtgcact cctggcagct gacgtagcag
      181 caccagtgga acacgcagcg gcacttctcc cggcgccgct ccgctcgcgc gttgtggccg
      241 cggccgcagc acagcaggtc gcagccgtcg atgccgtgcg agctgacgtt gcaggtgcgg
      301 tcgcgcgtgc cgaaggagcc cgtctcaggg ttgggctcgc agaagttggg cgaggcctcg
      361 tagtagacca ggtcgcgctc cgtgggcacc ttgaagtagg tgtagcgcgg ccgcagggtc
      421 tccacccagc cgcgggactc ccggtgcttc tccaccacca tctccgaggc gctgtcgtac
      481 ttgtccttga ggaagtcacc gatggcgcgg aagtcgggtt gcgaccacca gcatgtcttc
      541 acctcgcagc tgcccgacag cccgtggcac ttgcacttga ggtgcatgtg gctggcgatg
      601 gcctggcgcc cagcctcgt
//
