LOCUS       sequence-463755-       13575 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Split Flp on same plasmid; PhyA-Flp-397-Flp-396-FHY1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13575)
  AUTHORS   Tous C, Kinstlinger IS, Rice MEL, Deng J, Wong WW
  TITLE     Multiplexing light-inducible recombinases to control cell fate, 
            Boolean logic, and cell patterning in mammalian cells.
  JOURNAL   Sci Adv. 2025 May 9;11(19):eadt1971. doi: 10.1126/sciadv.adt1971. 
            Epub 2025 May 9.
  PUBMED    40344052
REFERENCE   2  (bases 1 to 13575)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Adv."; 
            date: "2025-05-9"; volume: "11(19):eadt1971. doi"; pages: " 
            10.1126/sciadv.adt1971. Epub 2025 May 9"
COMMENT     Sequence Label: CT769
FEATURES             Location/Qualifiers
     source          1..13575
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    272..389
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(674..718)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    903..1136
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    1633..1758
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             1805..2438
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     polyA_signal    2606..2740
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2630..2649)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     2684..2703
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(3165..3185)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     intron          3315..3380
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     promoter        complement(4514..4843)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4528..4663
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     4828..4848
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     primer_bind     complement(4918..4935)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5089..5677)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5169..5188)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5848..6639,6640..6708))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6471..6490
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6709..6813)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     6881..6899
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(6937..6959)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     7059..7078
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7292..7311
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     promoter        7601..7619
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     7601..7618
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     LTR             7644..7877
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     7967..7983
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     7968..7984
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    7987..8575
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             8093..8104
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     8502..8522
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8638..8658)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(10492..10548)
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             complement(10558..10578)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     regulatory      12549..12558
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(join(12595..13575,1..198))
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          12604..13542
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     complement(12627..12647)
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
ORIGIN
        1 aacgttcacg gcgactactg cacttatata cggttctccc ccaccctcgg gaaaaaggcg
       61 gagccagtac acgacatcac tttcccagtt taccccgcgc caccttctct aggcaccggt
      121 tcaattgccg acccctcccc ccaacttctc ggggactgtg ggcgatgtgc gctctgccca
      181 ctgacgggca ccggagcctc acgggctgca ggaattcgat atcaagctta tcgatccaaa
      241 ctggatctct gctgtccctg taataaaccc gaaaattttg aatttttgta atttgttttt
      301 gtaattcttt agtttgtatg tctgttgcta ttatgtctac tattctttcc cctgcactgt
      361 accccccaat cccccctttt cttttaaaat tgtggatgaa tactgccatt tggcgatacc
      421 gtcgagatcc gttcactaat cgaatggatc tgtctctgtc tctctctcca ccttcttctt
      481 ctattccttc gggcctgtcg ggtcccctcg gggttgggag gtgggtctga aacgataatg
      541 gtgaatatcc ctgcctaact ctattcacta tagaaagtac agcaaaaact attcttaaac
      601 ctaccaagcc tcctactatc attatgaata attttatata ccacagccaa tttgttatgt
      661 taaaccaatt ccacaaactt gcccatttat ctaattccaa taattcttgt tcattctttt
      721 cttgctggtt ttgcgattct tcaattaagg agtgtattaa gcttgtgtaa ttgttaattt
      781 ctctgtccca ctccatccag gtcgtgtgat tccaaatctg ttccagagat ttattactcc
      841 aactagcatt ccaaggcaca gcagtggtgc aaatgagttt tccagagcaa ccccaaatcc
      901 ccaggagctg ttgatccttt aggtatcttt ccacagccag gattcttgcc tggagctgct
      961 tgatgcccca gactgtgagt tgcaacagat gctgttgcgc ctcaatagcc ctcagcaaat
     1021 tgttctgctg ctgcactata ccagacaata attgtctggc ctgtaccgtc agcgtcattg
     1081 acgctgcgcc catagtgctt cctgctgctc ccaagaaccc aaggaacaaa gctcctattc
     1141 ccactgctct tttttctctc tgcaccactc ttctctttgc cttggtgggt gctactccta
     1201 atggttcaat ttttactact ttatatttat ataattcact tctccaattg tccctcatat
     1261 ctcctcctcc aggtctgaag atcagcggcc ggccgcttgc tgtgcggtgg tcttactttt
     1321 gttttgctct tcctctatct tgtctaaagc ttccttggtg tcttttatct ctatcctttg
     1381 atgcacacaa tagagggttg ctactgtatt atataatgat ctaagttctt ctgatcctgt
     1441 ctgaagggat ggttgtagct gtcccagtat ttgtctacag ccttctgatg tttctaacag
     1501 gccaggatta actgcgaatc gttctagctc cctgcttgcc catactatat gttttaattt
     1561 atattttttc tttccccctg gccttaaccg aattttttcc catcgcgatc taattctccc
     1621 ccgcttaata ctgacgctct cgcacccatc tctctccttc tagcctccgc tagtcaaaat
     1681 ttttggcgta ctcaccagtc gccgcccctc gcctcttgcc gtgcgcgctt cagcaagccg
     1741 agtcctgcgt cgagagagct cctctggttt ccctttcgct ttcaagtccc tgttcgggcg
     1801 ccactgctag agattttcca cactgactaa aagggtctga gggatctcta gttaccagag
     1861 tcacacaaca gacgggcaca cactacttga agcactcaag gcaagcttta ttgaggctta
     1921 agcagtgggt tccctagtta gccagagagc tcccaggctc agatctggtc taaccagaga
     1981 gacccagtac aggcaaaaag cagctgctta tatgcaggat ctgagggctc gccactcccc
     2041 agtcccgccc aggccacgcc tccctggaaa gtccccagcg gaaagtccct tgtagcaagc
     2101 tcgatatcag cagttcttga agtactccgg atgcagctct cgggccacgt gatgaaatgc
     2161 taggcggctg tcaaacctcc actctaacac ttctctctcc gggtcatcca tcccatgcag
     2221 gctcacaggg tgtaacaagc tggtgttctc tcctttattg gcctcttcta ccttatctgg
     2281 ctcaactggt actagcttgt agcaccatcc aaaggtcagt ggatatctga cccctggccc
     2341 tggtgtgtag ttctgctaat cagggaagta gccttgtgtg tggtagatcc acagatcaag
     2401 gatatcttgt cttctttggg agtgaattag cccttccaac tactaagttt gtagtacata
     2461 tttaacaaat acaatttctt taaaatgaaa ataattcaga ggaatcacag gtttagagta
     2521 aatgaaacca caggtaattg gcagtggtaa tagggtatgg ggtgggaagt ttgggatgat
     2581 tttggttagc ttgagttatc cagttgatcc agacatgata agatacattg atgagtttgg
     2641 acaaaccaca actagaatgc agtgaaaaaa atgctttatt tgtgaaattt gtgatgctat
     2701 tgctttattt gtaaccatta taagctgcaa taaacaagtt aacaacaaca attgcattca
     2761 ttttatgttt caggttcagg gggaggtgtg ggaggttttt taaagcaagt aaaacctcta
     2821 caaatgtggt atggctgatt atgatctcta gtcaaggcac tatacatcaa atattcctta
     2881 ttaacccctt tacaaattaa aaagctaaag gtacacaatt tttgagcata gttattaata
     2941 gcagacactc tatgcctgtg tggagtaaga aaaaacagta tgttatgatt ataactgtta
     3001 tgcctactta taaaggttac agaatatttt tccataattt tcttgtatag cagtgcagct
     3061 ttttcctttg tggtgtaaat agcaaagcaa gcaagagttc tattactaaa cacagcatga
     3121 ctcaaaaaac ttagcaattc tgaaggaaag tccttggggt cttctacctt tctcttcttt
     3181 tttggaggag tagaatgttg agagtcagca gtagcctcat catcactaga tggcatttct
     3241 tctgagcaaa acaggttttc ctcattaaag gcattccacc actgctccca ttcatcagtt
     3301 ccataggttg gaatctaaaa tacacaaaca attagaatca gtagtttaac acattataca
     3361 cttaaaaatt ttatatttac cttagagctt taaatctctg taggtagttt gtccaattat
     3421 gtcacaccac agaagtaagg ttccttcaca aagatccggg gcccactcat aaatccagtt
     3481 gccgccacgg tagccaatca ccgtatcgta taaatcatcg tcggtacgtt cggcatcgct
     3541 catcacaata cgtgcctgga cgtcgaggat ttcgcgtggg tcaatgccgc gccagatcca
     3601 catcagacgg ttaatcatgc gataccagtg agggatggtt ttaccatcaa gggccgactg
     3661 cacaggcggt tgtgcgccgt gattaaagcg gcggactagc gtcgaggttt caggatgttt
     3721 aaagcggggt ttgaacaggg tttcgctcag gtttgcctgt gtcatggatg cagcctccag
     3781 aatacttact ggaaactatt gtaacccgcc tgaagttaaa aagaacaacg cccggcagtg
     3841 ccaggcgttg aaaagattag cgaccggaga ttggcgggac gaatacgacg cccatatccc
     3901 acggctgttc aatccaggta tcttgcggga tatcaacaac atagtcatca accagcggac
     3961 gaccagccgg ttttgcgaag atggtgacaa agtgcgcttt tggatacatt tcacgaatcg
     4021 caaccgcagt accaccggta tccaccaggt catcaataac gatgaagcct tcgccatcgc
     4081 cttctgcgcg tttcagcact ttaagctcgc gctggttgtc gtgatcgtag ctggaaatac
     4141 aaacggtatc gacatgacga atacccagtt cacgcgccag taacgcaccc ggtacagacc
     4201 gccacggctt acggcaataa tgcctttcca ttgttcagaa ggcatcagtc ggcttgcgag
     4261 tttacgtgca tggatctgca acatgtccca ggtgacgatg tatttttcgc tcatgtgaag
     4321 tgtcccagcc tgtttatcta cggcttaaaa agtgttcgag gggaaaatag gttgcgcgag
     4381 attatagaga tctggcgcac taaaaaccag tatttcacat gagtccgcgt ctttttacgc
     4441 actgcctctc cctgacgcgg gataaagtgg tattctcaaa catatctcgc aagcctgtct
     4501 tgtgtccaag ctttttgcaa aagcctaggc ctccaaaaaa gcctcctcac tacttctgga
     4561 atagctcaga ggccgaggcg gcctcggcct ctgcataaat aaaaaaaatt agtcagccat
     4621 ggggcggaga atgggcggaa ctgggcggag ttaggggcgg gatgggcgga gttaggggcg
     4681 ggactatggt tgctgactaa ttgagatgca tgctttgcat acttctgcct gctggggagc
     4741 ctggggactt tccacacctg gttgctgact aattgagatg catgctttgc atacttctgc
     4801 ctgctgggga gcctggggac tttccacacc ctaactgaca cacattccac agctgcatta
     4861 atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc
     4921 gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa
     4981 ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa
     5041 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct
     5101 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac
     5161 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc
     5221 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc
     5281 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg
     5341 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga
     5401 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag
     5461 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta
     5521 cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag
     5581 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg
     5641 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac
     5701 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc
     5761 aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag
     5821 tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc
     5881 agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac
     5941 gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc
     6001 accggctcca gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg
     6061 tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag
     6121 tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc
     6181 acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac
     6241 atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag
     6301 aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac
     6361 tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg
     6421 agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc
     6481 gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact
     6541 ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg
     6601 atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa
     6661 tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt
     6721 tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg
     6781 tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga
     6841 cgtctaagaa accattatta tcatgacatt aacctataaa aataggcgta tcacgaggcc
     6901 ctttcgtctc gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc agctcccgga
     6961 gacggtcaca gcttgtctgt aagcggatgc cgggagcaga caagcccgtc agggcgcgtc
     7021 agcgggtgtt ggcgggtgtc ggggctggct taactatgcg gcatcagagc agattgtact
     7081 gagagtgcac cattcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta
     7141 gtaggttgag gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc
     7201 ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga
     7261 gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa
     7321 ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atctggctag
     7381 cgatgaccct gctgattggt tcgctgacca tttccgggtg cgggacggcg ttaccagaaa
     7441 ctcagaaggt tcgtccaacc aaaccgactc tgacggcagt ttacgagaga gatgataggg
     7501 tctgcttcag taagccagat gctacacaat taggcttgta catattgtcg ttagaacgcg
     7561 gctacaatta atacataacc ttatgtatca tacacatacg atttaggtga cactatagaa
     7621 tacacggaat taattctaga tgctgctaga gattttccac actgactaaa agggtctgag
     7681 ggatctctag ttaccagagt cacacaacag acgggcacac actacttgaa gcactcaagg
     7741 caagctttat tgaggcttaa gcagtgggtt ccctagttag ccagagagct cccaggctca
     7801 gatctggtct aaccagagag acccagtaca agcaaaaagc agatcttgtc ttcgttggga
     7861 gtgaattagc ccttccagtc cccccttttc ttttaaaaag tggctaagat ctacagctgc
     7921 cttgtaagtc attggtctta aaggtaccga gctcgaatta attccctcga ggtcgacggt
     7981 atcgatgcgg ggaggcggcc caaagggaga tccgactcgt ctgagggcga aggcgaagac
     8041 gcggaagagg ccgcagagcc ggcagcaggc cgcgggaagg aaggtccgct ggattgaggg
     8101 ccgaagggac gtagcagaag gacgtcccgc gcagaatcca ggtggcaaca caggcgagca
     8161 gccaaggaaa ggacgatgat ttccccgaca acaccacgga attgtcagtg cccaacagcc
     8221 gagcccctgt ccagcagcgg gcaaggcagg cggcgatgag ttccgccgtg gcaataggga
     8281 gggggaaagc gaaagtcccg gaaaggagct gacaggtggt ggcaatgccc caaccagtgg
     8341 gggttgcgtc agcaaacaca gtgcacacca cgccacgttg cctgacaacg ggccacaact
     8401 cctcataaag agacagcaac caggatttat acaaggagga gaaaatgaaa gccatacggg
     8461 aagcaatagc atgatacaaa ggcattaaag cagcgtatcc acatagcgta aaaggagcaa
     8521 catagttaag aataccagtc aatctttcac aaattttgta atccagaggt tgattatcga
     8581 taagcttgat atcaagcttg catgcctgca ggtcgactct agagtcgcgg ccgcttacac
     8641 cttccgcttt ttcttgggca gcattaaagt gctaaagtac tgctcgaatt cttggtcgat
     8701 ggtgggcttc ttggtttcgt gtaaagtgct atcatcttgg ttcacggtcc aacgaccaga
     8761 ggacagcacg aacttggcag cgttgctgct acagcccttg gcgtcctcct cctcctcttc
     8821 ttgctcgtcg aggtacagaa tgttctcgat caccttctcg gagaagtcct ccagattctg
     8881 ggtgccgtag atatagtcta aatgggactc gaagctgatc atcttgtcct ctccgcagct
     8941 gccgctggat tcttccttca tctcgatgtc gttgttgcac tccatggagg tggtggagtt
     9001 cacgtagctg gacatgctgt actcggtatc gatctcgctg ttgccgttgg tgttctcgct
     9061 acaagccacg ttggcaaagc atgtgtgttt ggataaaggt aataagctca gctcgtcgct
     9121 ctggtcggtc tggaacttcc tcttcttgga cacctccacc tcctccatct tcagcacgtt
     9181 cttgctgctg ctgataatca tgtggtggaa gctgttgatc tcgctgggct tctcgttgtt
     9241 gttgtcgacc tccacctcgg gggtaccgct gccaccactt gatccggagc cagaccctcc
     9301 ggagccctcg gcgctgccct tcagctgctc gatgtgctgc cactcctcga tggggttggt
     9361 ctcgtccttc agggcgatca tctccttgct gatggggtcg taggcgtagt acctggacac
     9421 cagggcgaag tagtggtcgg ggatggcggt gatctggtgg gtgtaggtgg tcctggccac
     9481 ggcggaggcc ctcttgtcgc tccagttgcc caccacgttt gtcagctcgg tcaggccctt
     9541 catgctcaga aagctggtca tcaggtgcct gccgatgtgg ctcttagggc cgttcttgat
     9601 agcgaagatg gggtaggggg cgttcttctt cagggccttg ttgtagctgc gcaccaggtt
     9661 gtccttcagc agctggtact cctgcttgtt gctgctgctg ttgccggtcc tgttcactct
     9721 cttcagcacg ggctcgctgt tcctcaggaa ctcgtccagg tacaccaggg ggtcgatcct
     9781 gcctctggcg ctgaaaaagt agatgtgcct ggacacgctt gtcttggtct cggtcaccag
     9841 gcactgaatg atcacgccca ggtacttgtt ctgcaccagc ttgaagctct tggggtccac
     9901 gttcttgatg tcgctgaacc tgccgcagtt gatgaatgtg gccaggaaca ggaactggta
     9961 cagggtcttg gtcttggtga acctgctggt gtactcgaag ctgttcagga tcttctcggt
    10021 gatctcccag atgctctcgc cctcggacag cagggccttc agcatcttct tgctgtggct
    10081 gttgcccttg tcggcctcct cgctgctctc gaactgcagc tgcaggctgg acacgatgtc
    10141 ggtgatgtcg ctctggtgct tctggccgtt gtaagggatg atggtgaact cccaggcggg
    10201 gatcagcttc ttcaggctgg cctccaggat ggtggccttc tgggtcttgt acttgaactg
    10261 caggctcttg ttcacgatgt cgaagctcag gctgttgctg atgatggtgt tgtagctcat
    10321 gaaggtggcc ctcttgatgg cggtgccgtt gtgggtgatc atccagcaca ggtaggtcag
    10381 ctcggcggca cagctggcga tcttctcgcc gctgggcctc tcgaatctct ccacgaactg
    10441 ccgcaccagc accttggggg gggtcttgca caggatgtcg aactggctca taggtccagg
    10501 gttctcctcc acgtctccag cctgcttcag caggctgaag ttagtagctc cgcttcccac
    10561 cttccgcttt ttcttgggga tccgcctgtt gatgtagctg ctcaggtagt ccagcacctc
    10621 ctggctgatg atgccgttcc aggcggggta tctgatgctg gtaccgctgc caccacttga
    10681 tccggagcca gaccctccgg attggattcc atcaatcttg agatcgttga gcttgctata
    10741 gatcaccttt gtgttcacgt cggtggtctc gctgtcctta aaggcgttac gtaagatgag
    10801 ctgtaaagag tggatggcat ccatttcgta gtccttccaa ggtaagcttc tggtcttcac
    10861 cacctccaga aaagccttga aggaggatct ggggtgcatc cttctggcat cgtccctatc
    10921 atcggggtcg tgtttggcgc cgccccatct cacttcgccg gcggtatggg aacgaaacca
    10981 gaaaatcata tctttgctgc tgatacgaac ggcagccatt ccgcagacgc tgtcgcctaa
    11041 agataaagcc ctaggaaagc cagcgtcgtg taaagaatcg gtgcttaaac cggtggaatc
    11101 catgtggtac tcacacagcc aagaagcgat ttcttgtaaa tggaactcgc taggggtggt
    11161 gcccagcttc cagatcttgt ccttgtataa taaggcggcg ccgtcgcact tgactaaatc
    11221 cataatattg ggggactggc tcacgatgcc gaggggagcg tccctcatga gcatgtcgca
    11281 gaggagtgtc tgggtacgta aaatgttctt ctccaccatc tggttgtcca gctcgacctc
    11341 cttgttgacg tggatggcaa acacttgggc taaaaattca caagcataac gtaagggaaa
    11401 agggacaaat ctgggggtgg tgttgtggca gaccactaaa ccccacagcc tctttctctt
    11461 ctgaggctgt gtggtggcat cgggagcatc gccctctcca tcctcctcat tcaccaccac
    11521 ggccatcact aaagaagcga tgctgtccat attggccatg tattgcagat gacaagagtg
    11581 gggagctctt aaagtgctgc cgcagagggt taaatcgaag ctcagcttct catcttgtaa
    11641 aaccctagcg tgcttggcgt tgcagtccac gatcattctc accttgttct tcataaataa
    11701 aaatctggca gcttggggga tgtctgtggc gggatagtgt aaacctaaat agggttctaa
    11761 accgggcttg gtcacttcgc tcaccacctc gccgtggtcg tcctcgtgga acttgtaggc
    11821 catgacacga tcgtaaccag tcagctcgaa gacttcttgc accatggtgt cgcacagcct
    11881 ctccatgctt ccgctgggta aagactggag tctggtgatg gccttggcgg ccagcttata
    11941 gctctgtaaa gctccggcgg ctgtcatggg cacttcatag ggcttaacgg gttcgaagtc
    12001 gatgatgatg gatccggtca ccctatggat aatggcgtag aagggcttgg cggatgtacg
    12061 acaatggacg aggatagggt tcagtaaaga cacgtcgccg aagccgagag ccttctgtaa
    12121 agcggaagcg ctgggagcgg taaataaaga tctgatatcg gtgccgatgc ccagaacggg
    12181 gtgctctccc acgctaggca cggcgtggga agccattgtg agcagttcgg aggcgttctc
    12241 ggagtaggcg atgaccttga aggtcttttc gtctaaagcc agtaaacatc caaagggctg
    12301 gatcagcttg cccttctgga tatgatggag gtaggttgtg gtgaccttgt cgctcctagg
    12361 aggctggttc tcgaccacgg gaccagtcac cctcacgctg gtggagtaat caaaggagga
    12421 gccgctctcc tcgaaatcgg cgtgcagttt ggcgtccacg gtggtctgag cgatgatacg
    12481 agcggagtga cgagatctac gagatccctc gctggactgt gtgggacgag atccgctcat
    12541 cttcttctcc atggtggcgg cacgcgtgag aattctcgag agctccagat cccctcacga
    12601 cacctgaaat ggaagaaaaa aactttgaac cactgtctga ggcttgagaa tgaaccaaga
    12661 tccaaactca aaaagggcaa attccaagga gaattacatc aagtgccaag ctggcctaac
    12721 ttcagtctcc acccactcag tgtggggaaa ctccatcgca taaaacccct ccccccaacc
    12781 taaagacgac gtactccaaa agctcgagaa ctaatcgagg tgcctggacg gcgcccggta
    12841 ctccgtggag tcacatgaag cgacggctga ggacggaaag gcccttttcc tttgtgtggg
    12901 tgactcaccc gcccgctctc ccgagcgccg cgtcctccat tttgagctcc ctgcagcagg
    12961 gccgggaagc ggccatcttt ccgctcacgc aactggtgcc gaccgggcca gccttgccgc
    13021 ccagggcggg gcgatacacg gcggcgcgag gccaggcacc agagcaggcc ggccagcttg
    13081 agactacccc cgtccgattc tcggtggccg cgctcgcagg ccccgcctcg ccgaacatgt
    13141 gcgctgggac gcacgggccc cgtcgccgcc cgcggcccca aaaaccgaaa taccagtgtg
    13201 cagatcttgg cccgcattta caagactatc ttgccagaaa aaaagcgtcg cagcaggtca
    13261 tcaaaaattt taaatggcta gagacttatc gaaagcagcg agacaggcgc gaaggtgcca
    13321 ccagattcgc acgcggcggc cccagcgccc aggccaggcc tcaactcaag cacgaggcga
    13381 aggggctcct taagcgcaag gcctcgaact ctcccaccca cttccaaccc gaagctcggg
    13441 atcaagaatc acgtactgca gccaggtgga agtaattcaa ggcacgcaag ggccataacc
    13501 cgtaaagagg ccaggcccgc gggaaccaca cacggcactt acctgtgttc tggcggcaaa
    13561 cccgttgcga aaaag
//
