LOCUS       sequence-463756-       10901 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  FLEx switch with tTA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10901)
  AUTHORS   Tous C, Kinstlinger IS, Rice MEL, Deng J, Wong WW
  TITLE     Multiplexing light-inducible recombinases to control cell fate, 
            Boolean logic, and cell patterning in mammalian cells.
  JOURNAL   Sci Adv. 2025 May 9;11(19):eadt1971. doi: 10.1126/sciadv.adt1971. 
            Epub 2025 May 9.
  PUBMED    40344052
REFERENCE   2  (bases 1 to 10901)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Adv."; 
            date: "2025-05-9"; volume: "11(19):eadt1971. doi"; pages: " 
            10.1126/sciadv.adt1971. Epub 2025 May 9"
COMMENT     Sequence Label: CT754
FEATURES             Location/Qualifiers
     source          1..10901
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     136..155
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(374..478)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     546..564
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(602..624)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     724..743
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     957..976
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     promoter        1266..1284
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     1266..1283
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     LTR             1309..1542
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     1632..1648
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     1633..1649
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    1652..2240
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             1758..1769
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     2167..2187
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     protein_bind    2300..2333
                     /label=FRT3
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision with other FRT3 sites
                     (Bosch et al., 2015)"
     protein_bind    2334..2367
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     CDS             2413..3036
                     /codon_start=1
                     /gene="tetR"
                     /product="tetracycline repressor TetR"
                     /label=TetR
                     /note="TetR binds to the tetracycline operator tetO to 
                     inhibit transcription. This inhibition can be relieved by 
                     adding tetracycline or doxycycline."
                     /translation="MMSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWH
                     VKNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGT
                     RPTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPT
                     TDSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     CDS             join(2416..3036,3037..3420)
                     /codon_start=1
                     /product="tetracycline-controlled transactivator, 
                     comprising a fusion of the tetracycline repressor TetR with
                     the C-terminal activation domain of herpes simplex virus 
                     VP16"
                     /label=tTA
                     /note="In the Tet-Off(R) system, tTA binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the absence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGSAYSRARTKNNYGSTI
                     EGLLDLPDDDAPEEAGLAAPRLSFLPAGHTRRLSTAPPTDVSLGDELHLDGEDVAMAHA
                     DALDDFDLDMLGDGDSPGPGFTPHDSAPYGALDMADFEFEQMFTDALGIDEYG"
     primer_bind     complement(2489..2508)
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
     protein_bind    complement(3421..3454)
                     /label=FRT3
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision with other FRT3 sites
                     (Bosch et al., 2015)"
     CDS             complement(3455..4165)
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(3733..3752)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     4002..4020
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     regulatory      4162..4171
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    complement(4211..4244)
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     promoter        complement(4278..4685)
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     misc_feature    4838..4955
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(5240..5284)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    5469..5702
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    6199..6324
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             6371..7004
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     polyA_signal    7172..7306
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7196..7215)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     7250..7269
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(7731..7751)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     intron          7881..7946
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     promoter        complement(9080..9409)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      9094..9229
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     9394..9414
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     primer_bind     complement(9484..9501)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9655..10243)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9735..9754)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10414..10901,1..304,305..373))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg
       61 gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc
      121 cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg
      181 gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga
      241 tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg
      301 ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat
      361 gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc
      421 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca
      481 catttccccg aaaagtgcca cctgacgtct aagaaaccat tattatcatg acattaacct
      541 ataaaaatag gcgtatcacg aggccctttc gtctcgcgcg tttcggtgat gacggtgaaa
      601 acctctgaca catgcagctc ccggagacgg tcacagcttg tctgtaagcg gatgccggga
      661 gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc tggcttaact
      721 atgcggcatc agagcagatt gtactgagag tgcaccattc gacgctctcc cttatgcgac
      781 tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg
      841 aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata
      901 cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg
      961 atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg
     1021 cgtccggcgt agaggatctg gctagcgatg accctgctga ttggttcgct gaccatttcc
     1081 gggtgcggga cggcgttacc agaaactcag aaggttcgtc caaccaaacc gactctgacg
     1141 gcagtttacg agagagatga tagggtctgc ttcagtaagc cagatgctac acaattaggc
     1201 ttgtacatat tgtcgttaga acgcggctac aattaataca taaccttatg tatcatacac
     1261 atacgattta ggtgacacta tagaatacac ggaattaatt ctagatgctg ctagagattt
     1321 tccacactga ctaaaagggt ctgagggatc tctagttacc agagtcacac aacagacggg
     1381 cacacactac ttgaagcact caaggcaagc tttattgagg cttaagcagt gggttcccta
     1441 gttagccaga gagctcccag gctcagatct ggtctaacca gagagaccca gtacaagcaa
     1501 aaagcagatc ttgtcttcgt tgggagtgaa ttagcccttc cagtcccccc ttttctttta
     1561 aaaagtggct aagatctaca gctgccttgt aagtcattgg tcttaaaggt accgagctcg
     1621 aattaattcc ctcgaggtcg acggtatcga tgcggggagg cggcccaaag ggagatccga
     1681 ctcgtctgag ggcgaaggcg aagacgcgga agaggccgca gagccggcag caggccgcgg
     1741 gaaggaaggt ccgctggatt gagggccgaa gggacgtagc agaaggacgt cccgcgcaga
     1801 atccaggtgg caacacaggc gagcagccaa ggaaaggacg atgatttccc cgacaacacc
     1861 acggaattgt cagtgcccaa cagccgagcc cctgtccagc agcgggcaag gcaggcggcg
     1921 atgagttccg ccgtggcaat agggaggggg aaagcgaaag tcccggaaag gagctgacag
     1981 gtggtggcaa tgccccaacc agtgggggtt gcgtcagcaa acacagtgca caccacgcca
     2041 cgttgcctga caacgggcca caactcctca taaagagaca gcaaccagga tttatacaag
     2101 gaggagaaaa tgaaagccat acgggaagca atagcatgat acaaaggcat taaagcagcg
     2161 tatccacata gcgtaaaagg agcaacatag ttaagaatac cagtcaatct ttcacaaatt
     2221 ttgtaatcca gaggttgatt atcgataagc ttgatatcaa gcttgcatgc ctgcaggtcg
     2281 actctagagt cgcggccgcg aagttcctat tcttcaaata gtataggaac ttcgaagttc
     2341 ctattctcta gaaagtatag gaacttcaag gtgcggccga tcgttgttgc tattagtacg
     2401 cgtgccgcca ccatgatgtc tagattagat aaaagtaaag tgattaacag cgcattagag
     2461 ctgcttaatg aggtcggaat cgaaggttta acaacccgta aactcgccca gaagctaggt
     2521 gtagagcagc ctacattgta ttggcatgta aaaaataagc gggctttgct cgacgcctta
     2581 gccattgaga tgttagatag gcaccatact cacttttgcc ctttagaagg ggaaagctgg
     2641 caagattttt tacgtaataa cgctaaaagt tttagatgtg ctttactaag tcatcgcgat
     2701 ggagcaaaag tacatttagg tacacggcct acagaaaaac agtatgaaac tctcgaaaat
     2761 caattagcct ttttatgcca acaaggtttt tcactagaga atgcattata tgcactcagc
     2821 gctgtggggc attttacttt aggttgcgta ttggaagatc aagagcatca agtcgctaaa
     2881 gaagaaaggg aaacacctac tactgatagt atgccgccat tattacgaca agctatcgaa
     2941 ttatttgatc accaaggtgc agagccagcc ttcttattcg gccttgaatt gatcatatgc
     3001 ggattagaaa aacaacttaa atgtgaaagt gggtccgcgt acagccgcgc gcgtacgaaa
     3061 aacaattacg ggtctaccat cgagggcctg ctcgatctcc cggacgacga cgcccccgaa
     3121 gaggcggggc tggcggctcc gcgcctgtcc tttctccccg cgggacacac gcgcagactg
     3181 tcgacggccc ccccgaccga tgtcagcctg ggggacgagc tccacttaga cggcgaggac
     3241 gtggcgatgg cgcatgccga cgcgctagac gatttcgatc tggacatgtt gggggacggg
     3301 gattccccgg gtccgggatt taccccccac gactccgccc cctacggcgc tctggatatg
     3361 gccgacttcg agtttgagca gatgtttacc gatgcccttg gaattgacga gtacggttaa
     3421 gaagttccta tactatttga agaataggaa cttcttactt gtacagctcg tccatgccgc
     3481 cggtggagtg gcggccctcg gcgcgttcgt actgttccac gatggtgtag tcctcgttgt
     3541 gggaggtgat gtccaacttg atgttgacgt tgtaggcgcc gggcagctgc acgggcttct
     3601 tggccttgta ggtggtcttg acctcagcgt cgtagtggcc gccgtccttc agcttcagcc
     3661 tctgcttgat ctcgcccttc agggcgccgt cctcggggta catccgctcg gaggaggcct
     3721 cccagcccat ggtcttcttc tgcattacgg ggccgtcgga ggggaagttg gtgccgcgca
     3781 gcttcacctt gtagatgaac tcgccgtcct gcagggagga gtcctgggtc acggtcacca
     3841 cgccgccgtc ctcgaagttc atcacgcgct cccacttgaa gccctcgggg aaggacagct
     3901 tcaagtagtc ggggatgtcg gcggggtgct tcacgtaggc cttggagccg tacatgaact
     3961 gaggggacag gatgtcccag gcgaagggca gggggccacc cttggtcacc ttcagcttgg
     4021 cggtctgggt gccctcgtag gggcggccct cgccctcgcc ctcgatctcg aactcgtggc
     4081 cgttcacgga gccctccatg tgcaccttga agcgcatgaa ctccttgatg atggccatgt
     4141 tatcctcctc gcccttgctc accatggtgg cggcggatcc cgaaggtaag gtcgaccgta
     4201 gtaggtaaag gaagttccta tactttctag agaataggaa cttcgcgcgt gagaattctc
     4261 gagagctcca gatccccccc gggcgactca gtctgtcgga ggactggcgc gccgagtgag
     4321 gggttgtgag ctcttttata gagctcggga agcagaagcg cgcgaacaga agcgagaagc
     4381 aggctgattg gttaattcaa ataaggcgca gggtcatttc aggtccttgg gggagcctgg
     4441 aaacatctga tgggtcttaa gaaactgctg agggttgggc catatctggg gaccatctgt
     4501 tcttggcctc gggccggggc cgaaactgcg gtgaccatct gttcttggcc ccgggccggg
     4561 gccgaaactg ctcaccgcag atatcctgtt tggcccaacg ttagctattt tcatgtaccc
     4621 gcccttgatc tgaacttctc tattcttggt ttggtatttt tccatgcctt gcaaaatggc
     4681 gttactgcag ctagcttgcc aaacctacag gtggggtctt tcattccccc ctttttctgg
     4741 agactaaata aaatctttta ttttatcggg gctgcaggaa ttcgatatca agcttatcga
     4801 tccaaactgg atctctgctg tccctgtaat aaacccgaaa attttgaatt tttgtaattt
     4861 gtttttgtaa ttctttagtt tgtatgtctg ttgctattat gtctactatt ctttcccctg
     4921 cactgtaccc cccaatcccc ccttttcttt taaaattgtg gatgaatact gccatttggc
     4981 gataccgtcg agatccgttc actaatcgaa tggatctgtc tctgtctctc tctccacctt
     5041 cttcttctat tccttcgggc ctgtcgggtc ccctcggggt tgggaggtgg gtctgaaacg
     5101 ataatggtga atatccctgc ctaactctat tcactataga aagtacagca aaaactattc
     5161 ttaaacctac caagcctcct actatcatta tgaataattt tatataccac agccaatttg
     5221 ttatgttaaa ccaattccac aaacttgccc atttatctaa ttccaataat tcttgttcat
     5281 tcttttcttg ctggttttgc gattcttcaa ttaaggagtg tattaagctt gtgtaattgt
     5341 taatttctct gtcccactcc atccaggtcg tgtgattcca aatctgttcc agagatttat
     5401 tactccaact agcattccaa ggcacagcag tggtgcaaat gagttttcca gagcaacccc
     5461 aaatccccag gagctgttga tcctttaggt atctttccac agccaggatt cttgcctgga
     5521 gctgcttgat gccccagact gtgagttgca acagatgctg ttgcgcctca atagccctca
     5581 gcaaattgtt ctgctgctgc actataccag acaataattg tctggcctgt accgtcagcg
     5641 tcattgacgc tgcgcccata gtgcttcctg ctgctcccaa gaacccaagg aacaaagctc
     5701 ctattcccac tgctcttttt tctctctgca ccactcttct ctttgccttg gtgggtgcta
     5761 ctcctaatgg ttcaattttt actactttat atttatataa ttcacttctc caattgtccc
     5821 tcatatctcc tcctccaggt ctgaagatca gcggccggcc gcttgctgtg cggtggtctt
     5881 acttttgttt tgctcttcct ctatcttgtc taaagcttcc ttggtgtctt ttatctctat
     5941 cctttgatgc acacaataga gggttgctac tgtattatat aatgatctaa gttcttctga
     6001 tcctgtctga agggatggtt gtagctgtcc cagtatttgt ctacagcctt ctgatgtttc
     6061 taacaggcca ggattaactg cgaatcgttc tagctccctg cttgcccata ctatatgttt
     6121 taatttatat tttttctttc cccctggcct taaccgaatt ttttcccatc gcgatctaat
     6181 tctcccccgc ttaatactga cgctctcgca cccatctctc tccttctagc ctccgctagt
     6241 caaaattttt ggcgtactca ccagtcgccg cccctcgcct cttgccgtgc gcgcttcagc
     6301 aagccgagtc ctgcgtcgag agagctcctc tggtttccct ttcgctttca agtccctgtt
     6361 cgggcgccac tgctagagat tttccacact gactaaaagg gtctgaggga tctctagtta
     6421 ccagagtcac acaacagacg ggcacacact acttgaagca ctcaaggcaa gctttattga
     6481 ggcttaagca gtgggttccc tagttagcca gagagctccc aggctcagat ctggtctaac
     6541 cagagagacc cagtacaggc aaaaagcagc tgcttatatg caggatctga gggctcgcca
     6601 ctccccagtc ccgcccaggc cacgcctccc tggaaagtcc ccagcggaaa gtcccttgta
     6661 gcaagctcga tatcagcagt tcttgaagta ctccggatgc agctctcggg ccacgtgatg
     6721 aaatgctagg cggctgtcaa acctccactc taacacttct ctctccgggt catccatccc
     6781 atgcaggctc acagggtgta acaagctggt gttctctcct ttattggcct cttctacctt
     6841 atctggctca actggtacta gcttgtagca ccatccaaag gtcagtggat atctgacccc
     6901 tggccctggt gtgtagttct gctaatcagg gaagtagcct tgtgtgtggt agatccacag
     6961 atcaaggata tcttgtcttc tttgggagtg aattagccct tccaactact aagtttgtag
     7021 tacatattta acaaatacaa tttctttaaa atgaaaataa ttcagaggaa tcacaggttt
     7081 agagtaaatg aaaccacagg taattggcag tggtaatagg gtatggggtg ggaagtttgg
     7141 gatgattttg gttagcttga gttatccagt tgatccagac atgataagat acattgatga
     7201 gtttggacaa accacaacta gaatgcagtg aaaaaaatgc tttatttgtg aaatttgtga
     7261 tgctattgct ttatttgtaa ccattataag ctgcaataaa caagttaaca acaacaattg
     7321 cattcatttt atgtttcagg ttcaggggga ggtgtgggag gttttttaaa gcaagtaaaa
     7381 cctctacaaa tgtggtatgg ctgattatga tctctagtca aggcactata catcaaatat
     7441 tccttattaa cccctttaca aattaaaaag ctaaaggtac acaatttttg agcatagtta
     7501 ttaatagcag acactctatg cctgtgtgga gtaagaaaaa acagtatgtt atgattataa
     7561 ctgttatgcc tacttataaa ggttacagaa tatttttcca taattttctt gtatagcagt
     7621 gcagcttttt cctttgtggt gtaaatagca aagcaagcaa gagttctatt actaaacaca
     7681 gcatgactca aaaaacttag caattctgaa ggaaagtcct tggggtcttc tacctttctc
     7741 ttcttttttg gaggagtaga atgttgagag tcagcagtag cctcatcatc actagatggc
     7801 atttcttctg agcaaaacag gttttcctca ttaaaggcat tccaccactg ctcccattca
     7861 tcagttccat aggttggaat ctaaaataca caaacaatta gaatcagtag tttaacacat
     7921 tatacactta aaaattttat atttacctta gagctttaaa tctctgtagg tagtttgtcc
     7981 aattatgtca caccacagaa gtaaggttcc ttcacaaaga tccggggccc actcataaat
     8041 ccagttgccg ccacggtagc caatcaccgt atcgtataaa tcatcgtcgg tacgttcggc
     8101 atcgctcatc acaatacgtg cctggacgtc gaggatttcg cgtgggtcaa tgccgcgcca
     8161 gatccacatc agacggttaa tcatgcgata ccagtgaggg atggttttac catcaagggc
     8221 cgactgcaca ggcggttgtg cgccgtgatt aaagcggcgg actagcgtcg aggtttcagg
     8281 atgtttaaag cggggtttga acagggtttc gctcaggttt gcctgtgtca tggatgcagc
     8341 ctccagaata cttactggaa actattgtaa cccgcctgaa gttaaaaaga acaacgcccg
     8401 gcagtgccag gcgttgaaaa gattagcgac cggagattgg cgggacgaat acgacgccca
     8461 tatcccacgg ctgttcaatc caggtatctt gcgggatatc aacaacatag tcatcaacca
     8521 gcggacgacc agccggtttt gcgaagatgg tgacaaagtg cgcttttgga tacatttcac
     8581 gaatcgcaac cgcagtacca ccggtatcca ccaggtcatc aataacgatg aagccttcgc
     8641 catcgccttc tgcgcgtttc agcactttaa gctcgcgctg gttgtcgtga tcgtagctgg
     8701 aaatacaaac ggtatcgaca tgacgaatac ccagttcacg cgccagtaac gcacccggta
     8761 cagaccgcca cggcttacgg caataatgcc tttccattgt tcagaaggca tcagtcggct
     8821 tgcgagttta cgtgcatgga tctgcaacat gtcccaggtg acgatgtatt tttcgctcat
     8881 gtgaagtgtc ccagcctgtt tatctacggc ttaaaaagtg ttcgagggga aaataggttg
     8941 cgcgagatta tagagatctg gcgcactaaa aaccagtatt tcacatgagt ccgcgtcttt
     9001 ttacgcactg cctctccctg acgcgggata aagtggtatt ctcaaacata tctcgcaagc
     9061 ctgtcttgtg tccaagcttt ttgcaaaagc ctaggcctcc aaaaaagcct cctcactact
     9121 tctggaatag ctcagaggcc gaggcggcct cggcctctgc ataaataaaa aaaattagtc
     9181 agccatgggg cggagaatgg gcggaactgg gcggagttag gggcgggatg ggcggagtta
     9241 ggggcgggac tatggttgct gactaattga gatgcatgct ttgcatactt ctgcctgctg
     9301 gggagcctgg ggactttcca cacctggttg ctgactaatt gagatgcatg ctttgcatac
     9361 ttctgcctgc tggggagcct ggggactttc cacaccctaa ctgacacaca ttccacagct
     9421 gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc
     9481 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca
     9541 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg
     9601 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca
     9661 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
     9721 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
     9781 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
     9841 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
     9901 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
     9961 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
    10021 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
    10081 cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
    10141 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
    10201 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
    10261 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag
    10321 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat
    10381 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc
    10441 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat
    10501 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc
    10561 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag
    10621 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag
    10681 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt
    10741 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg
    10801 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt
    10861 tgtcagaagt aagttggccg cagtgttatc actcatggtt a
//
