LOCUS       sequence-463757-       11197 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Dox-inducible VP64-MyoD.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11197)
  AUTHORS   Tous C, Kinstlinger IS, Rice MEL, Deng J, Wong WW
  TITLE     Multiplexing light-inducible recombinases to control cell fate, 
            Boolean logic, and cell patterning in mammalian cells.
  JOURNAL   Sci Adv. 2025 May 9;11(19):eadt1971. doi: 10.1126/sciadv.adt1971. 
            Epub 2025 May 9.
  PUBMED    40344052
REFERENCE   2  (bases 1 to 11197)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Adv."; 
            date: "2025-05-9"; volume: "11(19):eadt1971. doi"; pages: " 
            10.1126/sciadv.adt1971. Epub 2025 May 9"
COMMENT     Sequence Label: CT751
FEATURES             Location/Qualifiers
     source          1..11197
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     intron          487..552
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             682..702
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    1127..1261
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1164..1183)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1218..1237
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             1429..2062
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    2109..2234
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    2731..2964
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             3149..3193
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    3478..3595
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        3688..4066
                     /label=TRE3G promoter
                     /note="3rd-generation Tet-responsive promoter that can be 
                     activated by binding of Tet-On(R) 3G"
     protein_bind    3696..3714
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3732..3750
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3768..3786
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3804..3822
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3840..3858
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3876..3894
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3912..3930
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     3975..3999
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             4112..4132
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4142..4291
                     /codon_start=1
                     /product="tetrameric repeat of the minimal activation 
                     domain of herpes simplex virus VP16 (Beerli et al., 1998)"
                     /label=VP64
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDML"
     CDS             4298..4324
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             5315..5368
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             5375..6325
                     /codon_start=1
                     /product="near-infrared fluorescent protein with an 
                     emission peak at 720 nm, engineered from a bacterial 
                     phytochrome (Shcherbakova and Verkhusha, 2013)"
                     /label=iRFP720
                     /note="derived from Rhodopseudomonas palustris RpBphP2"
                     /translation="MAEGSVARQPDLLTCDDEPIHIPGAIQPHGLLLALAADMTIVAGS
                     DNLPELTGLAIGALIGRSAADVFDSETHNRLTIALAEPGAAVGAPITVGFTMRKDAGFI
                     GSWHRHDQLIFLELEPPQRDVAEPQAFFRRTNSAIRRLQAAETLESACAAAAQEVRKIT
                     GFDRVMIYRFASDFSGSVIAEDRCAEVESKLGLHYPASFIPAQARRLYTINPVRIIPDI
                     NYRPVPVTPDLNPVTGRPIDLSFAILRSVSPNHLEFMRNIGMHGTMSISILRGERLWGL
                     IVCHHRTPYYVDLDGRQACELVAQVLAWQIGVMEE"
     protein_bind    complement(6362..6395)
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     protein_bind    complement(6396..6429)
                     /label=FRT3
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision with other FRT3 sites
                     (Bosch et al., 2015)"
     misc_feature    6489..7077
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6542..6562)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6960..6971)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(7080..7096)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7081..7097)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             7187..7420
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(7445..7463)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(7446..7463)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(7753..7772)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(7986..8005)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8105..8127
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(8165..8183)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        8251..8355
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8356..8424,8425..9216)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8574..8593)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9387..9975
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9876..9895
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10129..10146
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(10216..10236)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        10221..10550
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      10401..10536
                     /label=SV40 ori
                     /note="SV40 origin of replication"
ORIGIN
        1 cggtcgctaa tcttttcaac gcctggcact gccgggcgtt gttcttttta acttcaggcg
       61 ggttacaata gtttccagta agtattctgg aggctgcatc catgacacag gcaaacctga
      121 gcgaaaccct gttcaaaccc cgctttaaac atcctgaaac ctcgacgcta gtccgccgct
      181 ttaatcacgg cgcacaaccg cctgtgcagt cggcccttga tggtaaaacc atccctcact
      241 ggtatcgcat gattaaccgt ctgatgtgga tctggcgcgg cattgaccca cgcgaaatcc
      301 tcgacgtcca ggcacgtatt gtgatgagcg atgccgaacg taccgacgat gatttatacg
      361 atacggtgat tggctaccgt ggcggcaact ggatttatga gtgggccccg gatctttgtg
      421 aaggaacctt acttctgtgg tgtgacataa ttggacaaac tacctacaga gatttaaagc
      481 tctaaggtaa atataaaatt tttaagtgta taatgtgtta aactactgat tctaattgtt
      541 tgtgtatttt agattccaac ctatggaact gatgaatggg agcagtggtg gaatgccttt
      601 aatgaggaaa acctgttttg ctcagaagaa atgccatcta gtgatgatga ggctactgct
      661 gactctcaac attctactcc tccaaaaaag aagagaaagg tagaagaccc caaggacttt
      721 ccttcagaat tgctaagttt tttgagtcat gctgtgttta gtaatagaac tcttgcttgc
      781 tttgctattt acaccacaaa ggaaaaagct gcactgctat acaagaaaat tatggaaaaa
      841 tattctgtaa cctttataag taggcataac agttataatc ataacatact gttttttctt
      901 actccacaca ggcatagagt gtctgctatt aataactatg ctcaaaaatt gtgtaccttt
      961 agctttttaa tttgtaaagg ggttaataag gaatatttga tgtatagtgc cttgactaga
     1021 gatcataatc agccatacca catttgtaga ggttttactt gctttaaaaa acctcccaca
     1081 cctccccctg aacctgaaac ataaaatgaa tgcaattgtt gttgttaact tgtttattgc
     1141 agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt
     1201 ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctggat
     1261 caactggata actcaagcta accaaaatca tcccaaactt cccaccccat accctattac
     1321 cactgccaat tacctgtggt ttcatttact ctaaacctgt gattcctctg aattattttc
     1381 attttaaaga aattgtattt gttaaatatg tactacaaac ttagtagttg gaagggctaa
     1441 ttcactccca aagaagacaa gatatccttg atctgtggat ctaccacaca caaggctact
     1501 tccctgatta gcagaactac acaccagggc caggggtcag atatccactg acctttggat
     1561 ggtgctacaa gctagtacca gttgagccag ataaggtaga agaggccaat aaaggagaga
     1621 acaccagctt gttacaccct gtgagcctgc atgggatgga tgacccggag agagaagtgt
     1681 tagagtggag gtttgacagc cgcctagcat ttcatcacgt ggcccgagag ctgcatccgg
     1741 agtacttcaa gaactgctga tatcgagctt gctacaaggg actttccgct ggggactttc
     1801 cagggaggcg tggcctgggc gggactgggg agtggcgagc cctcagatcc tgcatataag
     1861 cagctgcttt ttgcctgtac tgggtctctc tggttagacc agatctgagc ctgggagctc
     1921 tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa
     1981 gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag
     2041 tcagtgtgga aaatctctag cagtggcgcc cgaacaggga cttgaaagcg aaagggaaac
     2101 cagaggagct ctctcgacgc aggactcggc ttgctgaagc gcgcacggca agaggcgagg
     2161 ggcggcgact ggtgagtacg ccaaaaattt tgactagcgg aggctagaag gagagagatg
     2221 ggtgcgagag cgtcagtatt aagcggggga gaattagatc gcgatgggaa aaaattcggt
     2281 taaggccagg gggaaagaaa aaatataaat taaaacatat agtatgggca agcagggagc
     2341 tagaacgatt cgcagttaat cctggcctgt tagaaacatc agaaggctgt agacaaatac
     2401 tgggacagct acaaccatcc cttcagacag gatcagaaga acttagatca ttatataata
     2461 cagtagcaac cctctattgt gtgcatcaaa ggatagagat aaaagacacc aaggaagctt
     2521 tagacaagat agaggaagag caaaacaaaa gtaagaccac cgcacagcaa gcggccggcc
     2581 gctgatcttc agacctggag gaggagatat gagggacaat tggagaagtg aattatataa
     2641 atataaagta gtaaaaattg aaccattagg agtagcaccc accaaggcaa agagaagagt
     2701 ggtgcagaga gaaaaaagag cagtgggaat aggagctttg ttccttgggt tcttgggagc
     2761 agcaggaagc actatgggcg cagcgtcaat gacgctgacg gtacaggcca gacaattatt
     2821 gtctggtata gtgcagcagc agaacaattt gctgagggct attgaggcgc aacagcatct
     2881 gttgcaactc acagtctggg gcatcaagca gctccaggca agaatcctgg ctgtggaaag
     2941 atacctaaag gatcaacagc tcctggggat ttggggttgc tctggaaaac tcatttgcac
     3001 cactgctgtg ccttggaatg ctagttggag taataaatct ctggaacaga tttggaatca
     3061 cacgacctgg atggagtggg acagagaaat taacaattac acaagcttaa tacactcctt
     3121 aattgaagaa tcgcaaaacc agcaagaaaa gaatgaacaa gaattattgg aattagataa
     3181 atgggcaagt ttgtggaatt ggtttaacat aacaaattgg ctgtggtata taaaattatt
     3241 cataatgata gtaggaggct tggtaggttt aagaatagtt tttgctgtac tttctatagt
     3301 gaatagagtt aggcagggat attcaccatt atcgtttcag acccacctcc caaccccgag
     3361 gggacccgac aggcccgaag gaatagaaga agaaggtgga gagagagaca gagacagatc
     3421 cattcgatta gtgaacggat ctcgacggta tcgccaaatg gcagtattca tccacaattt
     3481 taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc
     3541 aacagacata caaactaaag aattacaaaa acaaattaca aaaattcaaa attttcgggt
     3601 ttattacagg gacagcagag atccagtttg gatcgataag cttgatatcg aattcctgca
     3661 gccccctttc gtcttcaaga attcctcgag tttactccct atcagtgata gagaacgtat
     3721 gaagagttta ctccctatca gtgatagaga acgtatgcag actttactcc ctatcagtga
     3781 tagagaacgt ataaggagtt tactccctat cagtgataga gaacgtatga ccagtttact
     3841 ccctatcagt gatagagaac gtatctacag tttactccct atcagtgata gagaacgtat
     3901 atccagttta ctccctatca gtgatagaga acgtataagc tttaggcgtg tacggtgggc
     3961 gcctataaaa gcagagctcg tttagtgaac cgtcagatcg cctggagcaa ttccacaaca
     4021 cttttgtctt ataccaactt tccgtaccac ttcctaccct cgtaaagggg ccgccaccat
     4081 gaaaactagt ggacaggccg gccaggctag cccgaaaaag aaacgcaaag ttgggcgcgc
     4141 cgacgcgctg gacgatttcg atctcgacat gctgggttct gatgccctcg atgactttga
     4201 cctggatatg ttgggaagcg acgcattgga tgactttgat ctggacatgc tcggctccga
     4261 tgctctggac gatttcgatc tcgatatgtt aattaactac ccgtacgacg ttccggacta
     4321 cgcttctgga ggcagtgggg gcggatcaat ggagcttcta tcgccgccac tccgggacat
     4381 agacttgaca ggccccgacg gctctctctg ctcctttgag acagcagacg acttctatga
     4441 tgatccgtgt ttcgactcac cagacctgcg cttttttgag gacctggacc cgcgcctggt
     4501 gcacgtggga gccctcctga aaccggagga gcacgcacac ttctctactg cggtgcaccc
     4561 aggcccaggc gctcgtgagg atgagcatgt gcgcgcgccc agcgggcacc accaggcggg
     4621 tcgctgcttg ctgtgggcct gcaaggcgtg caagcgcaag accaccaacg ctgatcgccg
     4681 caaggccgcc accatgcgcg agcgccgccg cctgagcaaa gtgaatgagg ccttcgagac
     4741 gctcaagcgc tgcacgtcca gcaacccgaa ccagcggcta cccaaggtgg agatcctgcg
     4801 caacgccatc cgctacatcg aaggtctgca ggctctgctg cgcgaccagg acgccgcgcc
     4861 ccctggcgcc gctgccttct acgcacctgg accgctgccc ccaggccgtg gcagcgagca
     4921 ctacagtggc gactcagacg cgtccagccc gcgctccaac tgctctgatg gcatgatgga
     4981 ttacagcggc cccccaagcg gcccccggcg gcagaatggc tacgacaccg cctactacag
     5041 tgaggcggtg cgcgagtcca ggccagggaa gagtgcggct gtgtcgagcc tcgactgcct
     5101 gtccagcata gtggagcgca tctccacaga cagccccgct gcgcctgcgc tgcttttggc
     5161 agatgcacca ccagagtcgc ctccgggtcc gccagagggg gcatccctaa gcgacacaga
     5221 acagggaacc cagaccccgt ctcccgacgc cgcccctcag tgtcctgcag gctcaaaccc
     5281 caatgcgatt tatcaggtgc ttatgcattc cggagagggc aggggaagtc ttctaacatg
     5341 cggggacgtg gaggaaaatc ccggcccagg taccatggcg gaaggatccg tcgccaggca
     5401 gcctgacctc ttgacctgcg acgatgagcc gatccatatc cccggtgcca tccaaccgca
     5461 tggactgctg ctcgccctcg ccgccgacat gacgatcgtt gccggcagcg acaaccttcc
     5521 cgaactcacc ggactggcga tcggcgccct gatcggccgc tctgcggccg atgtcttcga
     5581 ctcggagacg cacaaccgtc tgacgatcgc cttggccgag cccggggcgg ccgtcggagc
     5641 accgatcact gtcggcttca cgatgcgaaa ggacgcaggc ttcatcggct cctggcatcg
     5701 ccatgatcag ctcatcttcc tcgagctcga gcctccccag cgggacgtcg ccgagccgca
     5761 ggcgttcttc cgccgcacca acagcgccat ccgccgcctg caggccgccg aaaccttgga
     5821 aagcgcctgc gccgccgcgg cgcaagaggt gcggaagatt accggcttcg atcgggtgat
     5881 gatctatcgc ttcgcctccg acttcagcgg gtccgtgatc gcagaggatc ggtgcgccga
     5941 ggtcgagtca aaactaggcc tgcactatcc tgcctcattc atcccggcgc aggcccgtcg
     6001 gctctatacc atcaacccgg tacggatcat tcccgatatc aattatcggc cggtgccggt
     6061 caccccagac ctcaatccgg tcaccgggcg gccgattgat cttagcttcg ccatcctgcg
     6121 cagcgtctcg cccaaccatc tggagttcat gcgcaacata ggcatgcacg gcacgatgtc
     6181 gatctcgatt ttgcgcggcg agcgactgtg gggattgatc gtttgccatc accgaacgcc
     6241 gtactacgtc gatctcgatg gccgccaagc ctgcgagcta gtcgcccagg ttctggcctg
     6301 gcagatcggc gtgatggaag agtgaacgcg tactaatagc aacaacgatc ggccgcacct
     6361 tgaagttcct atactttcta gagaatagga acttcgaagt tcctatacta tttgaagaat
     6421 aggaacttcg cggccgcgac tctagagtcg acctgcaggc atgcaagctt gatatcaagc
     6481 ttatcgataa tcaacctctg gattacaaaa tttgtgaaag attgactggt attcttaact
     6541 atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat catgctattg
     6601 cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg tctctttatg
     6661 aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa
     6721 cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact ttcgctttcc
     6781 ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc tggacagggg
     6841 ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaaatcatcg tcctttcctt
     6901 ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac gtccttctgc tacgtccctt
     6961 cggccctcaa tccagcggac cttccttccc gcggcctgct gccggctctg cggcctcttc
     7021 cgcgtcttcg ccttcgccct cagacgagtc ggatctccct ttgggccgcc tccccgcatc
     7081 gataccgtcg acctcgaggg aattaattcg agctcggtac ctttaagacc aatgacttac
     7141 aaggcagctg tagatcttag ccacttttta aaagaaaagg ggggactgga agggctaatt
     7201 cactcccaac gaagacaaga tctgcttttt gcttgtactg ggtctctctg gttagaccag
     7261 atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc
     7321 ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga
     7381 tccctcagac ccttttagtc agtgtggaaa atctctagca gcatctagaa ttaattccgt
     7441 gtattctata gtgtcaccta aatcgtatgt gtatgataca taaggttatg tattaattgt
     7501 agccgcgttc taacgacaat atgtacaagc ctaattgtgt agcatctggc ttactgaagc
     7561 agaccctatc atctctctcg taaactgccg tcagagtcgg tttggttgga cgaaccttct
     7621 gagtttctgg taacgccgtc ccgcacccgg aaatggtcag cgaaccaatc agcagggtca
     7681 tcgctagcca gatcctctac gccggacgca tcgtggccgg catcaccggc gccacaggtg
     7741 cggttgctgg cgcctatatc gccgacatca ccgatgggga agatcgggct cgccacttcg
     7801 ggctcatgag cgcttgtttc ggcgtgggta tggtggcagg ccccgtggcc gggggactgt
     7861 tgggcgccat ctccttgcat gcaccattcc ttgcggcggc ggtgctcaac ggcctcaacc
     7921 tactactggg ctgcttccta atgcaggagt cgcataaggg agagcgtcga atggtgcact
     7981 ctcagtacaa tctgctctga tgccgcatag ttaagccagc cccgacaccc gccaacaccc
     8041 gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc
     8101 gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgagacga
     8161 aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag
     8221 acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa
     8281 atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat
     8341 tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg
     8401 gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa
     8461 gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt
     8521 gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt
     8581 ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat
     8641 tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg
     8701 acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta
     8761 cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat
     8821 catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag
     8881 cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa
     8941 ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca
     9001 ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc
     9061 ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt
     9121 atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc
     9181 gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat
     9241 atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt
     9301 tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac
     9361 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc
     9421 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca
     9481 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta
     9541 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct
     9601 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg
     9661 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc
     9721 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta
     9781 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg
     9841 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt
     9901 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg
     9961 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg
    10021 ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc
    10081 gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg
    10141 agcgaggaag cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt
    10201 cattaatgca gctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag
    10261 caggcagaag tatgcaaagc atgcatctca attagtcagc aaccaggtgt ggaaagtccc
    10321 caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccatag
    10381 tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc
    10441 cccatggctg actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc
    10501 tattccagaa gtagtgagga ggcttttttg gaggcctagg cttttgcaaa aagcttggac
    10561 acaagacagg cttgcgagat atgtttgaga ataccacttt atcccgcgtc agggagaggc
    10621 agtgcgtaaa aagacgcgga ctcatgtgaa atactggttt ttagtgcgcc agatctctat
    10681 aatctcgcgc aacctatttt cccctcgaac actttttaag ccgtagataa acaggctggg
    10741 acacttcaca tgagcgaaaa atacatcgtc acctgggaca tgttgcagat ccatgcacgt
    10801 aaactcgcaa gccgactgat gccttctgaa caatggaaag gcattattgc cgtaagccgt
    10861 ggcggtctgt accgggtgcg ttactggcgc gtgaactggg tattcgtcat gtcgataccg
    10921 tttgtatttc cagctacgat cacgacaacc agcgcgagct taaagtgctg aaacgcgcag
    10981 aaggcgatgg cgaaggcttc atcgttattg atgacctggt ggataccggt ggtactgcgg
    11041 ttgcgattcg tgaaatgtat ccaaaagcgc actttgtcac catcttcgca aaaccggctg
    11101 gtcgtccgct ggttgatgac tatgttgttg atatcccgca agatacctgg attgaacagc
    11161 cgtgggatat gggcgtcgta ttcgtcccgc caatctc
//
