LOCUS       sequence-463758-        6663 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Red light-inducible Cre-N; Cre-N251-PhyA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6663)
  AUTHORS   Tous C, Kinstlinger IS, Rice MEL, Deng J, Wong WW
  TITLE     Multiplexing light-inducible recombinases to control cell fate, 
            Boolean logic, and cell patterning in mammalian cells.
  JOURNAL   Sci Adv. 2025 May 9;11(19):eadt1971. doi: 10.1126/sciadv.adt1971. 
            Epub 2025 May 9.
  PUBMED    40344052
REFERENCE   2  (bases 1 to 6663)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Adv."; 
            date: "2025-05-9"; volume: "11(19):eadt1971. doi"; pages: " 
            10.1126/sciadv.adt1971. Epub 2025 May 9"
COMMENT     Sequence Label: CT368
FEATURES             Location/Qualifiers
     source          1..6663
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(104..123)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      917..1505
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1406..1425
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1659..1676
                     /label=L4440
                     /note="L4440 vector, forward primer"
     polyA_signal    1744..1878
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1768..1787)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     1822..1841
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      1898..2032
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     protein_bind    2117..2138
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2153..2158,2159..2176,2177..2183)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2191..2207
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2196..2218
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2215..2231
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2215..2231
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     2574..2593
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     polyA_signal    2592..2647
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     2693..2712
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    2952..3176
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     CDS             complement(3186..3206)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(4712..4731)
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     intron          4739..5755
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     complement(4793..4815)
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     promoter        complement(5756..6032)
                     /label=chicken beta-actin promoter
     enhancer        6034..6413
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        6444..6548
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6549..6617,6618..6663,1..746)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc
       61 gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat
      121 tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg
      181 acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag
      241 aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa
      301 cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc
      361 gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca
      421 cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc
      481 tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc
      541 tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg
      601 ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta
      661 tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag
      721 gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga
      781 ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc
      841 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa
      901 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
      961 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
     1021 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt
     1081 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
     1141 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
     1201 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
     1261 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
     1321 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
     1381 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
     1441 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
     1501 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
     1561 acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt
     1621 gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag
     1681 cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca
     1741 gcggatccag acatgataag atacattgat gagtttggac aaaccacaac tagaatgcag
     1801 tgaaaaaaat gctttatttg tgaaatttgt gatgctattg ctttatttgt aaccattata
     1861 agctgcaata aacaagttag ctttttgcaa aagcctaggc ctccaaaaaa gcctcctcac
     1921 tacttctgga atagctcaga ggccgaggcg gcctcggcct ctgcataaat aaaaaaaatt
     1981 agtcagccat ggggcggaga atgggcggaa ctgggcggag ttaggggcgg gactatggtt
     2041 gctgactaat tgagatgcgg atccgctggc acgacaggtt tcccgactgg aaagcgggca
     2101 gtgagcgcaa cgcaattaat gtgagttagc tcactcatta ggcaccccag gctttacact
     2161 ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa
     2221 acagctatga ccatgattac gccaagcttg ggctgcaggt cgagggatct ccataagaga
     2281 agagggacag ctatgactgg gagtagtcag gagaggagga aaaatctggc tagtaaaaca
     2341 tgtaaggaaa attttaggga tgttaaagaa aaaaataaca caaaacaaaa tataaaaaaa
     2401 atctaacctc aagtcaaggc ttttctatgg aataaggaat ggacagcagg gggctgtttc
     2461 atatactgat gacctcttta tagccaacct ttgttcatgg cagccagcat atgggcatat
     2521 gttgccaaac tctaaaccaa atactcattc tgatgtttta aatgatttgc cctcccatat
     2581 gtccttccga gtgagagaca caaaaaattc caacacacta ttgcaatgaa aataaatttc
     2641 ctttattagc cagaagtcag atgctcaagg ggcttcatga tgtccccata atttttggca
     2701 gagggaaaaa gatctcagtg gtatttgtga gccagggcat tggccacacc agccaccacc
     2761 ttctgatagg cagcctgcac ctgaggagtg cgctagcggt ggaagggctc ggagttgtgg
     2821 taatctatgt atcctgggcg gccgcgcttg gattctgcgt ttgtttccgt ctacgaactc
     2881 ccagcggtac acgctacagg gcgcgtgggg atacccccta gagccccagc tggttctttc
     2941 cgcctcagaa gccatagagc ccaccgcatc cccagcatgc ctgctattgt cttcccaatc
     3001 ctcccccttg ctgtcctgcc ccaccccacc ccccagaata gaatgacacc tactcagaca
     3061 atgcgatgca atttcctcat tttattagga aaggacagtg ggagtggcac cttccagggt
     3121 caaggaaggc acgggggagg ggcaaacaac agatggctgg caactagaag gcacaggaat
     3181 tctcacacct tccgcttttt cttgggcagc attaaagtgc taaagtactg ctcgaattct
     3241 tggtcgatgg tgggcttctt ggtttcgtgt aaagtgctat catcttggtt cacggtccaa
     3301 cgaccagagg acagcacgaa cttggcagcg ttgctgctac agcccttggc gtcctcctcc
     3361 tcctcttctt gctcgtcgag gtacagaatg ttctcgatca ccttctcgga gaagtcctcc
     3421 agattctggg tgccgtagat atagtctaaa tgggactcga agctgatcat cttgtcctct
     3481 ccgcagctgc cgctggattc ttccttcatc tcgatgtcgt tgttgcactc catggaggtg
     3541 gtggagttca cgtagctgga catgctgtac tcggtatcga tctcgctgtt gccgttggtg
     3601 ttctcgctac aagccacgtt ggcaaagcat gtgtgtttgg ataaaggtaa taagctcagc
     3661 tcgtcgctct ggtcggtctg gaacttcctc ttcttggaca cctccacctc ctccatcttc
     3721 agcacgttct tgctgctgct gataatcatg tggtggaagc tgttgatctc gctgggcttc
     3781 tcgttgttgt tgtcgacctc cacctcgggg gtaccgctgc caccacttga tccggagcca
     3841 gaccctccgg aagaaggggc agccacacca ttctttctga cccggcagaa caggtagttg
     3901 ttggggtcat cagccacacc agacacagag atccatctct ccaccagctt ggtaaccccc
     3961 agggacaggg ccttctccac accagctgtg gacaccaggg tcttggtcct gccaatgtgg
     4021 atcagcattc tcccaccatc ggtgcgggag atgtccttca ctctgattct ggcaatttcg
     4081 gcaatgcgca gcagggtgtt gtaggcaatg cccaggaagg ccaggttcct gatgtcctgg
     4141 catctgtcag agttctccat cagggatctg acttggtcaa agtcagtgcg ttcaaaggcc
     4201 agggcctgct tggctctctc cccagcatcc acattctcct ttctgattct cctcatcacc
     4261 agggacacag cattggagtc agaagggcga ggcaggccag atctcctgtg cagcatgttg
     4321 agctggccca ggtgctgttg gatggtcttc acagccaggc ctctggcttg caggtacagg
     4381 aggtagtccc tcacatcctc aggttcagca gggaaccatt tcctgttgtt cagcttgcac
     4441 caggcagccc aggatctgca cacagacagg agcatcttcc aggtgtgttc agagaaggcc
     4501 tgcctgtccc tgaacatgtc catcaggttc ttcctgactt catcagaggt ggcatccaca
     4561 gggagggcag gcaggttttg gtgcacagtc agcaggttgg acatggtggc ggcacgcgtg
     4621 cgagacgaga cgagacagcc tgagaatgga tgcgagtaat gggcgcgcct tcgctcgaat
     4681 taatcaattc tttgccaaaa tgatgagaca gcacaataac cagcacgttg cccaggagct
     4741 gtaggaaaaa gaagaaggca tgaacatggt tagcagaggc tctagagccg ccggtcacac
     4801 gccagaagcc gaaccccgcc ctgccccgtc ccccccgaag gcagccgtcc ccccgcggac
     4861 agccccgagg ctggagaggg agaaggggac ggcggcgcgg cgacgcacga aggccctccc
     4921 cgcccatttc cttcctgccg gcgccgcacc gcttcgcccc gcgcccgcta gagggggtgc
     4981 ggcggcgcct cccagatttc ggctccgcac agatttggga caaaggaagt ccctgcgccc
     5041 tctcgcacga ttaccataaa aggcaatggc tgcggctcgc cgcgcctcga cagccgccgg
     5101 cgctccgggg gccgccgcgc ccctcccccg agccctcccc ggcccgaggc ggccccgccc
     5161 cgcccggcac ccccacctgc cgccaccccc cgcccggcac ggcgagcccc gcgccacgcc
     5221 ccgtacggag ccccgcaccc gaagccgggc cgtgctcagc aactcgggga ggggggtgca
     5281 gggggggttg cagcccgacc gacgcgccca caccccctgc tcaccccccc acgcacacac
     5341 cccgcacgca gcctttgttc ccctcgcagc cccccccgca ccgcggggca ccgcccccgg
     5401 ccgcgctccc ctcgcgcaca ctgcggagcg cacaaagccc cgcgccgcgc ccgcagcgct
     5461 cacagccgcc gggcagcgcg gagccgcacg cggcgctccc cacgcacaca cacacgcacg
     5521 caccccccga gccgctcccc ccgcacaaag ggccctcccg gagcccctca aggctttcac
     5581 gcagccacag aaaagaaaca agccgtcatt aaaccaagcg ctaattacag cccggaggag
     5641 aagggccgtc ccgcccgctc acctgtggga gtaacgcggt cagtcagagc cggggcgggc
     5701 ggcgcgaggc ggcggcggag cggggcacgg ggcgaaggca gcgcgcagcg actcccgccc
     5761 gccgcgcgct tcgcttttta tagggccgcc gccgccgccg cctcgccata aaaggaaact
     5821 ttcggagcgc gccgctctga ttggctgccg ccgcacctct ccgcctcgcc ccgccccgcc
     5881 cctcgccccg ccccgccccg cctggcgcgc gccccccccc ccccccgccc ccatcgctgc
     5941 acaaaataat taaaaaataa ataaatacaa aattgggggt ggggaggggg gggagatggg
     6001 gagagtgaag cagaacgtgg ggctcacctc gaccatggta atagcgatga ctaatacgta
     6061 gatgtactgc caagtaggaa agtcccataa ggtcatgtac tgggcataat gccaggcggg
     6121 ccatttaccg tcattgacgt caataggggg cgtacttggc atatgataca cttgatgtac
     6181 tgccaagtgg gcagtttacc gtaaatactc cacccattga cgtcaatgga aagtccctat
     6241 tggcgttact atgggaacat acgtcattat tgacgtcaat gggcgggggt cgttgggcgg
     6301 tcagccaggc gggccattta ccgtaagtta tgtaacgcgg aactccatat atgggctatg
     6361 aactaatgac cccgtaattg attactatta ataactagtc aataatcaat gtcgacccag
     6421 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt
     6481 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa
     6541 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt
     6601 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt
     6661 tgg
//
