LOCUS       sequence-463768-       10318 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  To Express HA tagged hfCas13d the CMV promoter. Also encodes a 
            mCherry gene outside of the viral genome.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10318)
  AUTHORS   Back F, Sandoval A, Vu LM, Hong VM, Bhaskara A, Rodriguez SR, 
            O'Brien JT, Kolber BJ, Kroener S, Ploski JE
  TITLE     Adeno-associated viral vector resource for the RNA-targeting Cas13d:
            A comparison of high-fidelity variants, DjCas13d and hfCas13d.
  JOURNAL   Mol Ther Methods Clin Dev. 2025 Aug 20;33(4):101565. doi: 
            10.1016/j.omtm.2025.101565. eCollection 2025 Dec 11.
  PUBMED    41020266
REFERENCE   2  (bases 1 to 10318)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Ther 
            Methods Clin Dev."; date: "2025-08-20"; volume: "33(4):101565. doi";
            pages: " 10.1016/j.omtm.2025.101565. eCollection 2025 Dec 11"
COMMENT     Sequence Label: pAAV-CMV-intron-hfCas13d-pA/EF1a-mCherry-WPRE-pa
FEATURES             Location/Qualifiers
     source          1..10318
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     repeat_region   651..780
                     /label=AAV2 ITR (alternate)
                     /note="Functional equivalent of wild-type AAV2 ITR"
     enhancer        856..1159
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1160..1363
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     1314..1334
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     1360..1384
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          1512..1890
                     /label=chimeric intron
                     /note="fusion between human cytomegalovirus intron A and 
                     human beta-globin intron 2"
     CDS             1973..1993
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             2003..4900
                     /codon_start=1
                     /product="RNA-targeting Type VI CRISPR protein from 
                     Ruminococcus flavefaciens strain XPD3002 (Konermann et al.,
                     2018)"
                     /label=RfxCas13d
                     /translation="IEKKKSFAKGMGVKSTLVSGSKVYMTTFAEGSDARLEKIVEGDSI
                     RSVNEGEAFSAEMADKNAGYKIGNAKFSHPKGYAVVANNPLYTGPVQQDMLGLKETLEK
                     RYFGESADGNDNICIQVIHNILDIEKILVEYITNVVYVVNNISGLDKDIIGFGKFSTVY
                     TYDEFKDPEHHRAAFNNNDKLINAIKAQYDEFDNFLDNPRLGYFGQAFFSKEGRNYIIN
                     YGNECYDILALLSGLRHWVVHNNEEESRISRTWLYNLDKNLDNEYISTLNYLYDRITNE
                     LTNSFSKNSAANVNYIAETLGINPAEFAEQYFRFSIMKEQKNLGFNITKLREVMLDRKD
                     MSEIRKNHKVFDSIRTKVYTMMDFVIYRYYIEEDAKVAAANKSLPDNEKSLSEKDIFVI
                     NLRGSFNDDQKDALYYDEANRIWRKLENIMHNIKEFRGNKTREYKKKDAPRLPRILPAG
                     RDVSAFSKLMYALTMFLDGKEINDLLTTLINKFDNIQSFLKVMPLIGVNAKFVEEYAFF
                     KDSAKIADELRLIKSFARMGEPIADARRAMYIDAIRILGTNLSYDELKALADTFSLDEN
                     GNKLKKGKHGMRNFIINNVISNKRFHYLIRYGDPAHLHEIAKNEAVVKFVLGRIADIQK
                     KQGQNGKNQIDRYYETCIGKDKGKSVSEKVDALTKIITGMNYDQFDKKRSVIEDTGREN
                     AEREKFKKIISLYLTVIYHILKNIVNINARYVIGFHCVERDAQLYKEKGYDINLKKLEE
                     KGFSSVTKLCAGIDETAPDKRKDVEKEMAERAKESIDSLESANPKLYANYIKYSDEKKA
                     EEFTRQINREKAKTALNAYLRNTKWNVIIREDLLRIDNKTCTLFRNKAVHLEVARYVHA
                     YINDIAEVNSYFQLYHYIMQRIIMNERYEKSSGKVSEYFDAVNDEKKYNDRLLKLLCVP
                     FGYCIPRFKNLSIEALFDRNEAAKFDKEKKKVSGNS"
     CDS             4910..4930
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4940..4966
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     polyA_signal    4981..5029
                     /note="synthetic polyadenylation signal"
     repeat_region   5046..5186
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   5046..5175
                     /label=AAV2 ITR
     promoter        5199..6377
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          5430..6368
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     6325..6345
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             6390..7097
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="VSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEGT
                     QTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFE
                     DGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKG
                     EIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERAE
                     GRHSTGGMDELYK"
     primer_bind     complement(6532..6550)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     6800..6819
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     misc_feature    7269..7857
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7322..7342)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7740..7751)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    7889..8365
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(8088..8107)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     rep_origin      8446..8901
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8533..8552)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8743..8764
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(8918..8937)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     9037..9059
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(9097..9115)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        9183..9287
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9288..9356,9357..10148)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9506..9525)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt cctgcaggca
      661 gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt ggtcgcccgg
      721 cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact aggggttcct
      781 gcggccgcac gcgtctagtt attaatagta atcaattacg gggtcattag ttcatagccc
      841 atatatggag ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa
      901 cgacccccgc ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac
      961 tttccattga cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca
     1021 agtgtatcat atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg
     1081 gcattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt
     1141 agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg
     1201 gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg
     1261 gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat
     1321 gggcggtagg cgtgtacggt gggaggtcta tataagcaga gctcgtttag tgaaccgtca
     1381 gatcgcctgg agacgccatc cacgctgttt tgacctccat agaagacacc gggaccgatc
     1441 cagcctccgc ggattcgaat cccggccggg aacggtgcat tggaacgcgg attccccgtg
     1501 ccaagagtga cgtaagtacc gcctatagag tctataggcc cacaaaaaat gctttcttct
     1561 tttaatatac ttttttgttt atcttatttc taatactttc cctaatctct ttctttcagg
     1621 gcaataatga tacaatgtat catgcctctt tgcaccattc taaagaataa cagtgataat
     1681 ttctgggtta aggcaatagc aatatttctg catataaata tttctgcata taaattgtaa
     1741 ctgatgtaag aggtttcata ttgctaatag cagctacaat ccagctacca ttctgctttt
     1801 attttatggt tgggataagg ctggattatt ctgagtccaa gctaggccct tttgctaatc
     1861 atgttcatac ctcttatctt cctcccacag ctcctgggca acgtgctggt ctgtgtgctg
     1921 gcccatcact ttggcaaaga attgggattc gaacatcgat gccaccatga gccccaagaa
     1981 gaagagaaag gtggaggcca gcatcgaaaa aaaaaagtcc ttcgccaagg gcatgggcgt
     2041 gaagtccaca ctcgtgtccg gctccaaagt gtacatgaca accttcgccg aaggcagcga
     2101 cgccaggctg gaaaagatcg tggagggcga cagcatcagg agcgtgaatg agggcgaggc
     2161 cttcagcgct gaaatggccg ataaaaacgc cggctataag atcggcaacg ccaaattcag
     2221 ccatcctaag ggctacgccg tggtggctaa caaccctctg tatacaggac ccgtccagca
     2281 ggatatgctc ggcctgaagg aaactctgga aaagaggtac ttcggcgaga gcgctgatgg
     2341 caatgacaat atttgtatcc aggtgatcca taacatcctg gacattgaaa aaatcctcgt
     2401 ggaatacatt accaacgtgg tgtacgtggt caacaatatc tccggcctgg ataaggacat
     2461 tattggattc ggcaagttct ccacagtgta tacctacgac gaattcaaag accccgagca
     2521 ccatagggcc gctttcaaca ataacgataa gctcatcaac gccatcaagg cccagtatga
     2581 cgagttcgac aacttcctcg ataaccccag actcggctat ttcggccagg cctttttcag
     2641 caaggagggc agaaattaca tcatcaatta cggcaacgaa tgctatgaca ttctggccct
     2701 cctgagcgga ctgaggcact gggtggtcca taacaacgaa gaagagtcca ggatctccag
     2761 gacctggctc tacaacctcg ataagaacct cgacaacgaa tacatctcca ccctcaacta
     2821 cctctacgac aggatcacca atgagctgac caactccttc tccaagaact ccgccgccaa
     2881 cgtgaactat attgccgaaa ctctgggaat caaccctgcc gaattcgccg aacaatattt
     2941 cagattcagc attatgaaag agcagaaaaa cctcggattc aatatcacca agctcaggga
     3001 agtgatgctg gacaggaagg atatgtccga gatcaggaaa aatcataagg tgttcgactc
     3061 catcaggacc aaggtctaca ccatgatgga ctttgtgatt tataggtatt acatcgaaga
     3121 ggatgccaag gtggctgccg ccaataagtc cctccccgat aatgagaagt ccctgagcga
     3181 gaaggatatc tttgtgatta acctgagggg ctccttcaac gacgaccaga aggatgccct
     3241 ctactacgat gaagctaata gaatttggag aaagctcgaa aatatcatgc acaacatcaa
     3301 ggaatttagg ggaaacaaga caagagagta taagaagaag gacgccccta gactgcccag
     3361 aatcctgccc gctggccgtg atgtttccgc cttcagcaaa ctcatgtatg ccctgaccat
     3421 gttcctggat ggcaaggaga tcaacgacct cctgaccacc ctgattaata aattcgataa
     3481 catccagagc ttcctgaagg tgatgcctct catcggagtc aacgctaagt tcgtggagga
     3541 atacgccttt ttcaaagact ccgccaagat cgccgatgag ctgaggctga tcaagtcctt
     3601 cgctagaatg ggagaaccta ttgccgatgc caggagggcc atgtatatcg acgccatccg
     3661 tattttagga accaacctgt cctatgatga gctcaaggcc ctcgccgaca ccttttccct
     3721 ggacgagaac ggaaacaagc tcaagaaagg caagcacggc atgagaaatt tcattattaa
     3781 taacgtgatc agcaataaaa ggttccacta cctgatcaga tacggtgatc ctgcccacct
     3841 ccatgagatc gccaaaaacg aggccgtggt gaagttcgtg ctcggcagga tcgctgacat
     3901 ccagaaaaaa cagggccaga acggcaagaa ccagatcgac aggtactacg aaacttgtat
     3961 cggaaaggat aagggcaaga gcgtgagcga aaaggtggac gctctcacaa agatcatcac
     4021 cggaatgaac tacgaccaat tcgacaagaa aaggagcgtc attgaggaca ccggcaggga
     4081 aaacgccgag agggagaagt ttaaaaagat catcagcctg tacctcaccg tgatctacca
     4141 catcctcaag aatattgtca atatcaacgc caggtacgtc atcggattcc attgcgtcga
     4201 gcgtgatgct caactgtaca aggagaaagg ctacgacatc aatctcaaga aactggaaga
     4261 gaagggattc agctccgtca ccaagctctg cgctggcatt gatgaaactg cccccgataa
     4321 gagaaaggac gtggaaaagg agatggctga aagagccaag gagagcattg acagcctcga
     4381 gagcgccaac cccaagctgt atgccaatta catcaaatac agcgacgaga agaaagccga
     4441 ggagttcacc aggcagatta acagggagaa ggccaaaacc gccctgaacg cctacctgag
     4501 gaacaccaag tggaatgtga tcatcaggga ggacctcctg agaattgaca acaagacatg
     4561 taccctgttc agaaacaagg ccgtccacct ggaagtggcc aggtatgtcc acgcctatat
     4621 caacgacatt gccgaggtca attcctactt ccaactgtac cattacatca tgcagagaat
     4681 tatcatgaat gagaggtacg agaaaagcag cggaaaggtg tccgagtact tcgacgctgt
     4741 gaatgacgag aagaagtaca acgataggct cctgaaactg ctgtgtgtgc ctttcggcta
     4801 ctgtatcccc aggtttaaga acctgagcat cgaggccctg ttcgatagga acgaggccgc
     4861 caagttcgac aaggagaaaa agaaggtgtc cggcaattcc ggatccggac ctaagaaaaa
     4921 gaggaaggtg gcggccgctt acccatacga tgttccagat tacgcttagg ctaggaattc
     4981 aataaaagat ctttattttc attagatctg tgtgttggtt ttttgtgtgc ggaccgagcg
     5041 gccgcaggaa cccctagtga tggagttggc cactccctct ctgcgcgctc gctcgctcac
     5101 tgaggccggg cgaccaaagg tcgcccgacg cccgggcttt gcccgggcgg cctcagtgag
     5161 cgagcgagcg cgcagctgcc tgcaggggcg ccactagtgg ctccggtgcc cgtcagtggg
     5221 cagagcgcac atcgcccaca gtccccgaga agttgggggg aggggtcggc aattgaaccg
     5281 gtgcctagag aaggtggcgc ggggtaaact gggaaagtga tgtcgtgtac tggctccgcc
     5341 tttttcccga gggtggggga gaaccgtata taagtgcagt agtcgccgtg aacgttcttt
     5401 ttcgcaacgg gtttgccgcc agaacacagg taagtgccgt gtgtggttcc cgcgggcctg
     5461 gcctctttac gggttatggc ccttgcgtgc cttgaattac ttccacctgg ctgcagtacg
     5521 tgattcttga tcccgagctt cgggttggaa gtgggtggga gagttcgagg ccttgcgctt
     5581 aaggagcccc ttcgcctcgt gcttgagttg aggcctggcc tgggcgctgg ggccgccgcg
     5641 tgcgaatctg gtggcacctt cgcgcctgtc tcgctgcttt cgataagtct ctagccattt
     5701 aaaatttttg atgacctgct gcgacgcttt ttttctggca agatagtctt gtaaatgcgg
     5761 gccaagatct gcacactggt atttcggttt ttggggccgc gggcggcgac ggggcccgtg
     5821 cgtcccagcg cacatgttcg gcgaggcggg gcctgcgagc gcggccaccg agaatcggac
     5881 gggggtagtc tcaagctggc cggcctgctc tggtgcctgg cctcgcgccg ccgtgtatcg
     5941 ccccgccctg ggcggcaagg ctggcccggt cggcaccagt tgcgtgagcg gaaagatggc
     6001 cgcttcccgg ccctgctgca gggagctcaa aatggaggac gcggcgctcg ggagagcggg
     6061 cgggtgagtc acccacacaa aggaaaaggg cctttccgtc ctcagccgtc gcttcatgtg
     6121 actccacgga gtaccgggcg ccgtccaggc acctcgatta gttctcgagc ttttggagta
     6181 cgtcgtcttt aggttggggg gaggggtttt atgcgatgga gtttccccac actgagtggg
     6241 tggagactga agttaggcca gcttggcact tgatgtaatt ctccttggaa tttgcccttt
     6301 ttgagtttgg atcttggttc attctcaagc ctcagacagt ggttcaaagt ttttttcttc
     6361 catttcaggt gtcgtgaggt accggatccg tgagcaaggg cgaggaggat aacatggcca
     6421 tcatcaagga gttcatgcgc ttcaaggtgc acatggaggg ctccgtgaac ggccacgagt
     6481 tcgagatcga gggcgagggc gagggccgcc cctacgaggg cacccagacc gccaagctga
     6541 aggtgaccaa gggtggcccc ctgcccttcg cctgggacat cctgtcccct cagttcatgt
     6601 acggctccaa ggcctacgtg aagcaccccg ccgacatccc cgactacttg aagctgtcct
     6661 tccccgaggg cttcaagtgg gagcgcgtga tgaacttcga ggacggcggc gtggtgaccg
     6721 tgacccagga ctcctccctg caggacggcg agttcatcta caaggtgaag ctgcgcggca
     6781 ccaacttccc ctccgacggc cccgtaatgc agaagaagac catgggctgg gaggcctcct
     6841 ccgagcggat gtaccccgag gacggcgccc tgaagggcga gatcaagcag aggctgaagc
     6901 tgaaggacgg cggccactac gacgctgagg tcaagaccac ctacaaggcc aagaagcccg
     6961 tgcagctgcc cggcgcctac aacgtcaaca tcaagttgga catcacctcc cacaacgagg
     7021 actacaccat cgtggaacag tacgaacgcg ccgagggccg ccactccacc ggcggcatgg
     7081 acgagctgta caagtaaggc gcgccccccc ctaacgttac tggccgaagc cgcttggaat
     7141 aaggccggtg tgcgtttgtc tatatgttat tttccaccat attgccgtct tttggcaatg
     7201 tgagggcccg gaaacctggc cctgtcttct tgacgagcat tcctaaattc gatatcaagc
     7261 ttatcgataa tcaacctctg gattacaaaa tttgtgaaag attgactggt attcttaact
     7321 atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat catgctattg
     7381 cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg tctctttatg
     7441 aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa
     7501 cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact ttcgctttcc
     7561 ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc tggacagggg
     7621 ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaaatcatcg tcctttcctt
     7681 ggctgctcgc ctatgttgcc acctggattc tgcgcgggac gtccttctgc tacgtccctt
     7741 cggccctcaa tccagcggac cttccttccc gcggcctgct gccggctctg cggcctcttc
     7801 cgcgtcttcg ccttcgccct cagacgagtc ggatctccct ttgggccgcc tccccgcatc
     7861 gataccgagc gctgctcgag agatctacgg gtggcatccc tgtgacccct ccccagtgcc
     7921 tctcctggcc ctggaagttg ccactccagt gcccaccagc cttgtcctaa taaaattaag
     7981 ttgcatcatt ttgtctgact aggtgtcctt ctataatatt atggggtgga ggggggtggt
     8041 atggagcaag gggcaagttg ggaagacaac ctgtagggcc tgcggggtct attgggaacc
     8101 aagctggagt gcagtggcac aatcttggct cactgcaatc tccgcctcct gggttcaagc
     8161 gattctcctg cctcagcctc ccgagttgtt gggattccag gcatgcatga ccaggctcag
     8221 ctaatttttg tttttttggt agagacgggg tttcaccata ttggccaggc tggtctccaa
     8281 ctcctaatct caggtgatct acccaccttg gcctcccaaa ttgctgggat tacaggcgtg
     8341 aaccactgct cccttccctg tccttactag tggcgcctga tgcggtattt tctccttacg
     8401 catctgtgcg gtatttcaca ccgcatacgt caaagcaacc atagtacgcg ccctgtagcg
     8461 gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg
     8521 ccttagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc
     8581 cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc
     8641 tcgaccccaa aaaacttgat ttgggtgatg gttcacgtag tgggccatcg ccctgataga
     8701 cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa
     8761 ctggaacaac actcaactct atctcgggct attcttttga tttataaggg attttgccga
     8821 tttcggtcta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattttaaca
     8881 aaatattaac gtttacaatt ttatggtgca ctctcagtac aatctgctct gatgccgcat
     8941 agttaagcca gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc
     9001 tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt
     9061 tttcaccgtc atcaccgaaa cgcgcgagac gaaagggcct cgtgatacgc ctatttttat
     9121 aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg
     9181 tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga
     9241 gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac
     9301 atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc
     9361 cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca
     9421 tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc
     9481 caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg
     9541 ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac
     9601 cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca
     9661 taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg
     9721 agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac
     9781 cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg
     9841 caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat
     9901 taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg
     9961 ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg
    10021 cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc
    10081 aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc
    10141 attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt
    10201 tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt
    10261 aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttc
//
