LOCUS       sequence-46378-e         635 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 635)
  TITLE     Photo-activated Caspase-7
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 635)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: L57V
FEATURES             Location/Qualifiers
     source          1..635
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    1..17
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        37..146
                     /label=p10 promoter
                     /note="baculovirus promoter for expression in insect cells"
     CDS             173..601
                     /codon_start=1
                     /gene="NPH1 from Avena sativa"
                     /product="light, oxygen, or voltage domain from phototropin
                     1 of Avena sativa (Herrou and Crosson, 2011)"
                     /label=LOV2
                     /note="photoswitch that undergoes a light-inducible 
                     conformational change"
                     /translation="LATTLERIEKNFVITDPRLPDNPIIFASDSFLQLTEYSREEILGR
                     NCRFLQGPETDRATVRKIRDAIDNQTEVTVQLINYTKSGKKFWNLFHLQPMRDQKGDVQ
                     YFIGVQLDGTEHVRDAAEREGVMLIKKTAENIDEAAKEL"
ORIGIN
        1 ttgtgagcgg ataacaattc cccggagtta atccgggacc tttaattcaa cccaacacaa
       61 tatattatag ttaaataaga attattatca aatcatttgt atattaatta aaatactata
      121 ctgtaaatta cattttattt acaatcaaag gagatatacc atggggacta gtttggctac
      181 tacacttgaa cgtattgaga agaactttgt cattactgac ccaagattgc cagataatcc
      241 cattatattc gcgtccgata gtttcttgca gttgacagaa tatagccgtg aagaaatttt
      301 gggaagaaac tgcaggtttc tacaaggtcc tgaaactgat cgcgcgacag tgagaaaaat
      361 tagagatgcc atagataacc aaacagaggt cactgttcag ctgattaatt atacaaagag
      421 tggtaaaaag ttctggaacc tctttcactt gcagcctatg cgagatcaga agggagatgt
      481 ccagtacttt attggggttc agttggatgg aactgagcat gtccgagatg ctgccgagag
      541 agagggagtc atgctgatta agaaaactgc agaaaatatt gatgaggcgg caaaagaact
      601 tgctagtgga tccacatatc agtacaacat gaatt
//
