LOCUS       sequence-463780-        3332 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Vector part for BEVA; BEVA2.0 Position 3 pMB1 narrow broad host 
            range origin of replication with oriT, CmR..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3332)
  AUTHORS   Geddes BA, Williamson R, Schumacher J, Ardi A, Levin G, Cervenka E, 
            Huang R, diCenzo G
  TITLE     BEVA2.0: modular assembly of golden gate-compatible vectors with 
            expanded utility for genetic engineering.
  JOURNAL   Can J Microbiol. 2025 Jan 1;71:1-13. doi: 10.1139/cjm-2024-0246.
  PUBMED    39919271
REFERENCE   2  (bases 1 to 3332)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Can J 
            Microbiol."; date: "2025-01-1"; volume: "71:1-13. doi"; pages: " 
            10.1139/cjm-2024-0246"
COMMENT     Sequence Label: pNDGG007
FEATURES             Location/Qualifiers
     source          1..3332
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(392..410)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             complement(521..1180)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1095..1114
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(1181..1283)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     rep_origin      1384..1972
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2126..2143
                     /label=L4440
                     /note="L4440 vector, forward primer"
     promoter        2402..2420
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     2402..2419
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     promoter        2500..2518
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      2655..3243
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     oriT            join(3313..3332,1..89)
                     /note="incP origin of transfer"
ORIGIN
        1 gattcccgtt gagcaccgcc aggtgcgaat aagggacagt gaagaaggaa cacccgctcg
       61 cgggtgggcc tacttcacct atcctgcccg gctgacgccg ttggatacac caaggaaagt
      121 ctacacgaac cctttggcaa aatcctgtat atcgtgcgaa aaaggatgga tataccgaaa
      181 aaatcgctat aatgaccccg aagcagggtt atgcagcgga aaagtagtcg agacgcatct
      241 ttctagaaga tctcctacaa tattctcagc tgccatggaa aatcgatgtt cttcttttat
      301 tctctcaaga ttttcaggct gtatattaac cattcctgta gacttcttaa ttaagacgtc
      361 agaattctcg aggcggccgc atgtgagtct ccctatagtg agtcgtatta atttcgcggg
      421 cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagtag
      481 caccaggcgt ttaagggcac caataactgc cttaaaaaaa ttacgccccg ccctgccact
      541 catcgcagta ctgttgtaat tcattaagca ttctgccgac atggaagcca tcacaaacgg
      601 catgatgaac ctgaatcgcc agcggcatca gcaccttgtc gccttgcgta taatatttgc
      661 ccatggtgaa aacgggggcg aagaagttgt ccatattggc cacgtttaaa tcaaaactgg
      721 tgaaactcac ccagggattg gctgagacaa aaaacatatt ctcaataaac cctttaggga
      781 aataggccag gttttcaccg taacacgcca catcttgcga atatatgtgt agaaactgcc
      841 ggaaatcgtc gtggtattca ctccagagcg atgaaaacgt ttcagtttgc tcatggaaaa
      901 cggtgtaaca agggtgaaca ctatcccata tcaccagctc accgtctttc attgccatac
      961 gaaattccgg atgagcattc atcaggcggg caagaatgtg aataaaggcc ggataaaact
     1021 tgtgcttatt tttctttacg gtctttaaaa aggccgtaat atccagctga acggtctggt
     1081 tataggtaca ttgagcaact gactgaaatg cctcaaaatg ttctttacga tgccattggg
     1141 atatatcaac ggtggtatat ccagtgattt ttttctccat tttagcttcc ttagctcctg
     1201 aaaatctcga taactcaaaa aatacgcccg gtagtgatct tatttcatta tggtgaaagt
     1261 tggaacctct tacgtgccga tcaaagtctc attttcgcca aaagttgtca tgaccaaaat
     1321 cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc
     1381 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct
     1441 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg
     1501 cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca
     1561 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc
     1621 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga
     1681 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac
     1741 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga
     1801 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag
     1861 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg
     1921 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag
     1981 caatgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc
     2041 tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc
     2101 tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagccg aaaaatcaat
     2161 aatcagacaa caagatgtgc gaactcgata ttttacacga ctctctttac caattctgcc
     2221 ccgaattaca cttaaaacga ctcaacagct taacgttggc ttgccacgca ttacttgact
     2281 gtaaaactct cactcttacc gaacttggcc gtaacctgcc aaccaaagcg agaacaaaac
     2341 ataacatcaa acgaatcgac cgattgttag gtaatcgtca cctgcaggaa ggtttaaacg
     2401 catttaggtg acactataga agtgtgtatc gctcgaggga tccgaattcg aagtcttggt
     2461 acggagacac ttgtgcctga acaccatatc catccggcgt aatacgactc actataggga
     2521 gagcggccgc cagatcttcc ggatggctcg agtttttcag caagatgcgt ctcagtaatc
     2581 atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag
     2641 atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa
     2701 aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg
     2761 aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag
     2821 ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg
     2881 ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga
     2941 tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc
     3001 ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc
     3061 acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga
     3121 gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt
     3181 cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg
     3241 aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac
     3301 aagctagctt atcggccagc ctcgcagagc ag
//
