LOCUS       sequence-463782-        8821 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  To Express a GFP human Tau P301L fusion protein from a CMV promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8821)
  TITLE     Ploski Lab Plasmids 2025
REFERENCE   2  (bases 1 to 8821)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pLenti-CMV-eGFP-hTau P301L-WPRE
FEATURES             Location/Qualifiers
     source          1..8821
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     743..760
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    877..898
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(913..918,919..936,937..943)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    951..967
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     956..978
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     975..991
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     975..991
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     1010..1030
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        1012..1030
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        1058..1284
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             1285..1465
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1512..1637
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    2130..2363
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2548..2592
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2859..2976
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        2999..3302
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3303..3506
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     3457..3477
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     3503..3527
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        3737..3755
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     3737..3754
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     3768..3787
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      3831..3840
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(3837..3839,3840..3842,3843..4553)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(3837..3839,3840..3842,3843..4553)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3882..3903)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4143..4162)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4490..4511
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    5729..6317
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5782..5802)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6200..6211)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6397..6630
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6702..6823
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6739..6758)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6793..6812
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6863..6998
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(7018..7037)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7019..7037)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7047..7064)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7047..7063)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7056..7078)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      7205..7660
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7292..7311)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7502..7523
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7686..7790
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7791..7859,7860..8651)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8009..8028)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
      781 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt
      841 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta
      901 ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg
      961 ataacaattt cacacaggaa acagctatga ccatgattac gccaagcgcg caattaaccc
     1021 tcactaaagg gaacaaaagc tggagctgca agcttaatgt agtcttatgc aatactcttg
     1081 tagtcttgca acatggtaac gatgagttag caacatgcct tacaaggaga gaaaaagcac
     1141 cgtgcatgcc gattggtgga agtaaggtgg tacgatcgtg ccttattagg aaggcaacag
     1201 acgggtctga catggattgg acgaaccact gaattgccgc attgcagaga tattgtattt
     1261 aagtgcctag ctcgatacat aaacgggtct ctctggttag accagatctg agcctgggag
     1321 ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt
     1381 caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt
     1441 tagtcagtgt ggaaaatctc tagcagtggc gcccgaacag ggacttgaaa gcgaaaggga
     1501 aaccagagga gctctctcga cgcaggactc ggcttgctga agcgcgcacg gcaagaggcg
     1561 aggggcggcg actggtgagt acgccaaaaa ttttgactag cggaggctag aaggagagag
     1621 atgggtgcga gagcgtcagt attaagcggg ggagaattag atcgcgatgg gaaaaaattc
     1681 ggttaaggcc agggggaaag aaaaaatata aattaaaaca tatagtatgg gcaagcaggg
     1741 agctagaacg attcgcagtt aatcctggcc tgttagaaac atcagaaggc tgtagacaaa
     1801 tactgggaca gctacaacca tcccttcaga caggatcaga agaacttaga tcattatata
     1861 atacagtagc aaccctctat tgtgtgcatc aaaggataga gataaaagac accaaggaag
     1921 ctttagacaa gatagaggaa gagcaaaaca aaagtaagac caccgcacag caagcggccg
     1981 ctgatcttca gacctggagg aggagatatg agggacaatt ggagaagtga attatataaa
     2041 tataaagtag taaaaattga accattagga gtagcaccca ccaaggcaaa gagaagagtg
     2101 gtgcagagag aaaaaagagc agtgggaata ggagctttgt tccttgggtt cttgggagca
     2161 gcaggaagca ctatgggcgc agcgtcaatg acgctgacgg tacaggccag acaattattg
     2221 tctggtatag tgcagcagca gaacaatttg ctgagggcta ttgaggcgca acagcatctg
     2281 ttgcaactca cagtctgggg catcaagcag ctccaggcaa gaatcctggc tgtggaaaga
     2341 tacctaaagg atcaacagct cctggggatt tggggttgct ctggaaaact catttgcacc
     2401 actgctgtgc cttggaatgc tagttggagt aataaatctc tggaacagat ttggaatcac
     2461 acgacctgga tggagtggga cagagaaatt aacaattaca caagcttaat acactcctta
     2521 attgaagaat cgcaaaacca gcaagaaaag aatgaacaag aattattgga attagataaa
     2581 tgggcaagtt tgtggaattg gtttaacata acaaattggc tgtggtatat aaaattattc
     2641 ataatgatag taggaggctt ggtaggttta agaatagttt ttgctgtact ttctatagtg
     2701 aatagagtta ggcagggata ttcaccatta tcgtttcaga cccacctccc aaccccgagg
     2761 ggacccgaca ggcccgaagg aatagaagaa gaaggtggag agagagacag agacagatcc
     2821 attcgattag tgaacggatc tcgacggtat cggttaactt ttaaaagaaa aggggggatt
     2881 ggggggtaca gtgcagggga aagaatagta gacataatag caacagacat acaaactaaa
     2941 gaattacaaa aacaaattac aaaaattcaa aattttatcg ataagcttgg gagttccgcg
     3001 ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga
     3061 cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat
     3121 gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa
     3181 gtacgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca
     3241 tgaccttatg ggactttcct acttggcagt acatctacgt attagtcatc gctattacca
     3301 tggtgatgcg gttttggcag tacatcaatg ggcgtggata gcggtttgac tcacggggat
     3361 ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg
     3421 actttccaaa atgtcgtaac aactccgccc cattgacgca aatgggcggt aggcgtgtac
     3481 ggtgggaggt ctatataagc agagctcgtt tagtgaaccg tcagatcgcc tggagacgcc
     3541 atccacgctg ttttgacctc catagaagac accgggaccg atccagcctc cgcggccggg
     3601 aacggtgcat tggaacgcgg attccccgtg ccaagagtga cgtaagtacc gcctatagag
     3661 tctataggcc cacccccttg gcttcgttag aacgcggcta caattaatac ataaccttat
     3721 gtatcataca catacgattt aggtgacact atagaataac atccactttg cctttctctc
     3781 cacaggtgtc cactcccagg tccaactgca cctcggttct atcgatggtc gccaccatgg
     3841 tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat cctggtcgag ctggacggcg
     3901 acgtaaacgg ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc acctacggca
     3961 agctgaccct gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg
     4021 tgaccaccct gacctacggc gtgcagtgct tcagccgcta ccccgaccac atgaagcagc
     4081 acgacttctt caagtccgcc atgcccgaag gctacgtcca ggagcgcacc atcttcttca
     4141 aggacgacgg caactacaag acccgcgccg aggtgaagtt cgagggcgac accctggtga
     4201 accgcatcga gctgaagggc atcgacttca aggaggacgg caacatcctg gggcacaagc
     4261 tggagtacaa ctacaacagc cacaacgtct atatcatggc cgacaagcag aagaacggca
     4321 tcaaggtgaa cttcaagatc cgccacaaca tcgaggacgg cagcgtgcag ctcgccgacc
     4381 actaccagca gaacaccccc atcggcgacg gccccgtgct gctgcccgac aaccactacc
     4441 tgagcaccca gtccgccctg agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc
     4501 tggagttcgt gaccgccgcc gggatcactc tcggcatgga cgagctgtac aagggatccg
     4561 ctgagccccg ccaggagttc gaagtgatgg aagatcacgc tgggacgtac gggttggggg
     4621 acaggaaaga tcaggggggc tacaccatgc accaagacca agagggtgac acggacgctg
     4681 gcctgaaagc tgaagaagca ggcattggag acacccccag cctggaagac gaagctgctg
     4741 gtcacgtgac ccaagctcgc atggtcagta aaagcaaaga cgggactgga agcgatgaca
     4801 aaaaagccaa gggggctgat ggtaaaacga agatcgccac accgcgggga gcagcccctc
     4861 caggccagaa gggccaggcc aacgccacca ggattccagc aaaaaccccg cccgctccaa
     4921 agacaccacc cagctctggt gaacctccaa aatcagggga tcgcagcggc tacagcagcc
     4981 ccggctcccc aggcactccc ggcagccgct cccgcacccc gtcccttcca accccaccca
     5041 cccgggagcc caagaaggtg gcagtggtcc gtactccacc caagtcgccg tcttccgcca
     5101 agagccgcct gcagacagcc cccgtgccca tgccagacct gaagaatgtc aagtccaaga
     5161 tcggctccac tgagaacctg aagcaccagc cgggaggcgg gaaggtgcag ataattaata
     5221 agaagctgga tcttagcaac gtccagtcca agtgtggctc aaaggataat atcaaacacg
     5281 tcctgggagg cggcagtgtg caaatagtct acaaaccagt tgacctgagc aaggtgacct
     5341 ccaagtgtgg ctcattaggc aacatccatc ataaaccagg aggtggccag gtggaagtaa
     5401 aatctgagaa gcttgacttc aaggacagag tccagtcgaa gattgggtcc ctggacaata
     5461 tcacccacgt ccctggcgga ggaaataaaa agattgaaac ccacaagctg accttccgcg
     5521 agaacgccaa agccaagaca gaccacgggg cggagatcgt gtacaagtcg ccagtggtgt
     5581 ctggggacac gtctccacgg catctcagca atgtctcctc caccggcagc atcgacatgg
     5641 tagactcgcc ccagctcgcc acgctagctg acgaggtgtc tgcctccctg gccaagcagg
     5701 gtttgtgaac cggttagtaa tgatcgacaa tcaacctctg gattacaaaa tttgtgaaag
     5761 attgactggt attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat
     5821 gcctttgtat catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc
     5881 ctggttgctg tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg
     5941 cactgtgttt gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct
     6001 ttccgggact ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct
     6061 tgcccgctgc tggacagggg ctcggctgtt gggcactgac aattccgtgg tgttgtcggg
     6121 gaagctgacg tcctttccat ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac
     6181 gtccttctgc tacgtccctt cggccctcaa tccagcggac cttccttccc gcggcctgct
     6241 gccggctctg cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc ggatctccct
     6301 ttgggccgcc tccccgcctg gcgatggtac ctttaagacc aatgacttac aaggcagctg
     6361 tagatcttag ccacttttta aaagaaaagg ggggactgga agggctaatt cactcccaac
     6421 gaagacaaga tctgcttttt gcttgtactg ggtctctctg gttagaccag atctgagcct
     6481 gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag
     6541 tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac
     6601 ccttttagtc agtgtggaaa atctctagca gtagtagttc atgtcatctt attattcagt
     6661 atttataact tgcaaagaaa tgaatatcag agagtgagag gaacttgttt attgcagctt
     6721 ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac
     6781 tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggctctagc
     6841 tatcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc
     6901 gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga
     6961 gctattccag aagtagtgag gaggcttttt tggaggccta gggacgtacc caattcgccc
     7021 tatagtgagt cgtattacgc gcgctcactg gccgtcgttt tacaacgtcg tgactgggaa
     7081 aaccctggcg ttacccaact taatcgcctt gcagcacatc cccctttcgc cagctggcgt
     7141 aatagcgaag aggcccgcac cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa
     7201 tgggacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg
     7261 accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc
     7321 gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga
     7381 tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt
     7441 gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat
     7501 agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat
     7561 ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa
     7621 tttaacgcga attttaacaa aatattaacg cttacaattt aggtggcact tttcggggaa
     7681 atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca
     7741 tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc
     7801 aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc
     7861 acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt
     7921 acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt
     7981 ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg
     8041 ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact
     8101 caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg
     8161 ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga
     8221 aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg
     8281 aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa
     8341 tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac
     8401 aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc
     8461 cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca
     8521 ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga
     8581 gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta
     8641 agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc
     8701 atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc
     8761 cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt
     8821 c
//
