LOCUS       sequence-463792-        3092 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pMOLC360_VR_I7 (Chloramphenicol R) containing the insert I7 to be 
            assembled in POC1519 (pMOBK360_VM) using the site-selective 
            methylation protection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3092)
  AUTHORS   Flores-Fernandez CN, Lin D, Robins K, O'Callaghan CA
  TITLE     UniClo: scarless hierarchical DNA assembly without sequence 
            constraint.
  JOURNAL   Nucleic Acids Res. 2025 Jun 20;53(12):gkaf548. doi: 
            10.1093/nar/gkaf548.
  PUBMED    40548934
REFERENCE   2  (bases 1 to 3092)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "2025-06-20"; volume: "53(12):gkaf548. doi"; 
            pages: " 10.1093/nar/gkaf548"
COMMENT     Sequence Label: POC1541
FEATURES             Location/Qualifiers
     source          1..3092
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1479..1581
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             1582..2241
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(1648..1667)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      2267..2812
                     /direction=RIGHT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     2713..2732
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2929..2946
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 cactctgtgg tgcagtacct ctcacgacta ggtctcacca ttacatatgc caagtaccca
       61 gaaagatgtc tggaacatgg caacacccac cacagaagta gtagctgcaa ttattgcctc
      121 cttaaaacag tggagatgtc acccatcggg gctagagaga atttgggaaa tcctgatctg
      181 cttctggttt caggtgggga atcctgtagg accagcagga acacggctgc ctcagtgcag
      241 ctggcacagg gcactgccag cccagagcaa ctcagagctc agaacactct ggggtccgaa
      301 gtcacaatgt cacagtagcc cccatgacca agagagacgg ttccagaagt gcagggcaac
      361 tccatatgac atgccggaag tattagctca aatcaacctt gtcctgtcta accacttact
      421 ctactgatcg gatgaggccc atccacatta ttgatggtaa ttttcttttt gtaaagtcag
      481 ctaattcagc atggggaagg gggtcatggt agacatgggg gagggctggt ctctccacct
      541 cctcacacta ggctaacagg gacacagaca cattcagatg cctttgcaga ctgaatcatc
      601 ctcgcatagc caggatcttc cttttgtgtt taattgggtc gtggtaggaa actgaagttc
      661 aaataaatag gttgttgtga atgttaataa aatacatcct attgatacat ggaaatactg
      721 aaatcttaaa accaaataac actgaatatc ttttagttaa tttagaataa ataaaaatta
      781 aagtgtagta attcattctc tcttttgaag cgctattgtc tattgttgta taataaataa
      841 catgaagttt gacaacccaa aacaacaaat acttatcatc tcccacagtt tccagtggtc
      901 aggaatctgg gagagatttc cttgagtgct tctggctcag ggcctctcac aaggtttatt
      961 gggggacaca cctgtcagag aatatgggga gggagccaga gaaccctggg aaaagtggca
     1021 catccagagg caaggccgac tccagtcctg gacaaaagga aagaagggtt gttggacgca
     1081 tcctagacca caggcaatct gaggagagtt gagcaaggcc atggaggagt cctccagcca
     1141 cagatggcca acagaggagt ctcctgttgc ccaggaatgg tctgtcttag tgtccctgct
     1201 gttacgtgtc agtggctggg aacagcccat gggaagcagg gcctctgcac caatgctgct
     1261 gagaatgaca gagcacggga gggaggcctt gggaaatttc ctggaaatga gactgagacc
     1321 tagtcgtgag aggtactgca ccacgaagtg tgcggccgcc ttcagcggcc gcacgaataa
     1381 atacctgtga cggaagatca cttcgcagaa taaataaatc ctggtgtccc tgttgatacc
     1441 gggaagccct gggccaactt ttggcgaaaa tgagacgttg atcggcacgt aagaggttcc
     1501 aactttcacc ataatgaaat aagatcacta ccgggcgtat tttttgagtt atcgagattt
     1561 tcaggagcta aggaagctaa aatggagaaa aaaatcactg gatataccac cgttgatata
     1621 tcccaatggc atcgtaaaga acattttgag gcatttcagt cagttgctca atgtacctat
     1681 aaccagaccg ttcagctgga tattacggcc tttttaaaga ccgtaaagaa aaataagcac
     1741 aagttttatc cggcctttat tcacattctt gcccgcctga tgaatgctca tccggagttc
     1801 cgtatggcaa tgaaagacgg tgagctggtg atatgggata gtgttcaccc ttgttacacc
     1861 gttttccatg agcaaactga aacgttttca tcgctctgga gtgaatacca cgacgatttc
     1921 cggcagtttc tacacatata ttcgcaagat gtggcgtgtt acggtgaaaa cctggcctat
     1981 ttccctaaag ggtttattga gaatatgttt ttcgtctcag ccaatccctg ggtgagtttc
     2041 accagttttg atttaaacgt ggccaatatg gacaacttct tcgcccccgt tttcaccatg
     2101 ggcaaatatt atacgcaagg cgacaaggtg ctgatgccgc tggcgattca ggttcatcat
     2161 gccgtttgtg atggcttcca tgtcggcaga atgcttaatg aattacaaca gtactgcgat
     2221 gagtggcagg gcggggcgta agagcctgtt taataagatg atcttcttga gatcgttttg
     2281 gtctgcgcgt aatctcttgc tctgaaaacg aaaaaaccgc cttgcagggc ggtttttcga
     2341 aggttctctg agctaccaac tctttgaacc gaggtaactg gcttggagga gcgcagtcac
     2401 caaaacttgt cctttcagtt tagccttaac cggcgcatga cttcaagact aactcctcta
     2461 aatcaattac cagtggctgc tgccagtggt gcttttgcat gtctttccgg gttggactca
     2521 agacgatagt taccggataa ggcgcagcgg tcggactgaa cggggggttc gtgcatacag
     2581 tccagcttgg agcgaactgc ctacccggaa ctgagtgtca ggcgtggaat gagacaaacg
     2641 cggccataac agcggaatga caccggtaaa ccgaaaggca ggaacaggag agcgcacgag
     2701 ggagccgcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccaccactg
     2761 atttgagcgt cagatttcgt gatgcttgtc aggggggcgg agcctatgga aaaacggctt
     2821 tgccgcggcc ctctcacttc cctgttaagt atcttcctgg catcttccag gaaatctccg
     2881 ccccgttcgt aagccatttc cgctcgccgc agtcgaacga ccgagcgtag cgagtcagtg
     2941 agcgaggaag cggaatatat cctgtatcac atattctgct gacgcaccgg tgcagccttt
     3001 tttctcctgc cacatgaagc acttcactga caccctcatc agtgccaaca tagtaagcca
     3061 gtatacactc cgctagcgcg caagcggccg ca
//
