LOCUS       sequence-463797-       18628 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pCCI vector encoding YFV-17D lacking C, prM, E but with mScarlet.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 18628)
  AUTHORS   Li Y, Xu W
  TITLE     Xu Lab plasmids 2025
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 18628)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: pCCI-YFV-Delta-CME-mScarlet
FEATURES             Location/Qualifiers
     source          1..18628
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      496..715
                     /label=ori2
                     /note="secondary origin of replication for the bacterial F 
                     plasmid; also known as oriS"
     rep_origin      783..1314
                     /label=oriV
                     /note="incP origin of replication"
     rep_origin      791..1405
                     /label=oriV
                     /note="origin of replication for the bacterial F plasmid"
     promoter        2263..2365
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             2366..3025
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(2432..2451)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     protein_bind    3245..3266
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(3281..3286,3287..3304,3305..3311)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3319..3335
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     3324..3346
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     3343..3359
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3343..3359
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        3796..3814
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     3796..3813
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     CDS             4013..4705
                     /codon_start=1
                     /product="bright monomeric red fluorescent protein evolved 
                     from a synthetic template (Bindels et al., 2016)"
                     /label=mScarlet
                     /translation="VSKGEAVIKEFMRFKVHMEGSMNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFSWDILSPQFMYGSRAFTKHPADIPDYYKQSFPEGFKWERVMNFEDGGA
                     VTVTQDTSLEDGTLIYKVKLRGTNFPPDGPVMQKKTMGWEASTERLYPEDGVLKGDIKM
                     ALRLKDGGRYLADFKTTYKAKKPVQMPGAYNVDRKLDITSHNEDYTVVEQYERSEGRHS
                     TGGMDELYK"
     primer_bind     complement(4143..4161)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     4411..4430
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     13578..13597
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     primer_bind     complement(13961..13980)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(13962..13980)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(13987..14004)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(13987..14003)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(13996..14018)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(14211..14230)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     protein_bind    14298..14331
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     misc_feature    14349..14747
                     /label=cos
                     /note="lambda cos site; allows packaging into phage lambda 
                     particles"
     misc_feature    15007..15480
                     /gene="sopC"
                     /label=sopC
                     /note="centromere-like partitioning region of the bacterial
                     F plasmid"
     CDS             complement(15553..16524)
                     /codon_start=1
                     /gene="sopB"
                     /product="partitioning protein for the bacterial F plasmid"
                     /label=sopB
                     /translation="MKRAPVIPKHTLNTQPVEDTSLSTPAAPMVDSLIARVGVMARGNA
                     ITLPVCGRDVKFTLEVLRGDSVEKTSRVWSGNERDQELLTEDALDDLIPSFLLTGQQTP
                     AFGRRVSGVIEIADGSRRRKAAALTESDYRVLVGELDDEQMAALSRLGNDYRPTSAYER
                     GQRYASRLQNEFAGNISALADAENISRKIITRCINTAKLPKSVVALFSHPGELSARSGD
                     ALQKAFTDKEELLKQQASNLHEQKKAGVIFEAEEVITLLTSVLKTSSASRTSLSSRHQF
                     APGATVLYKGDKMVLNLDRSRVPTECIEKIEAILKELEKPAP"
     CDS             complement(16524..17699)
                     /codon_start=1
                     /gene="sopA"
                     /product="partitioning protein for the bacterial F plasmid"
                     /label=sopA
                     /translation="MFRMKLMETLNQCINAGHEMTKAIAIAQFNDDSPEARKITRRWRI
                     GEAADLVGVSSQAIRDAEKAGRLPHPDMEIRGRVEQRVGYTIEQINHMRDVFGTRLRRA
                     EDVFPPVIGVAAHKGGVYKTSVSVHLAQDLALKGLRVLLVEGNDPQGTASMYHGWVPDL
                     HIHAEDTLLPFYLGEKDDVTYAIKPTCWPGLDIIPSCLALHRIETELMGKFDEGKLPTD
                     PHLMLRLAIETVAHDYDVIVIDSAPNLGIGTINVVCAADVLIVPTPAELFDYTSALQFF
                     DMLRDLLKNVDLKGFEPDVRILLTKYSNSNGSQSPWMEEQIRDAWGSMVLKNVVRETDE
                     VGKGQIRMRTVFEQAIDQRSSTGAWRNALSIWEPVCNEIFDRLIKPRWEIR"
     misc_feature    18025..18275
                     /gene="incC"
                     /label=incC
                     /note="incompatibility region of the bacterial F plasmid"
     CDS             complement(join(18278..18628,1..405))
                     /codon_start=1
                     /gene="repE"
                     /product="replication initiation protein for the bacterial 
                     F plasmid"
                     /label=repE
                     /translation="MAETAVINHKKRKNSPRIVQSNDLTEAAYSLSRDQKRMLYLFVDQ
                     IRKSDGTLQEHDGICEIHVAKYAEIFGLTSAEASKDIRQALKSFAGKEVVFYRPEEDAG
                     DEKGYESFPWFIKRAHSPSRGLYSVHINPYLIPFFIGLQNRFTQFRLSETKEITNPYAM
                     RLYESLCQYRKPDGSGIVSLKIDWIIERYQLPQSYQRMPDFRRRFLQVCVNEINSRTPM
                     RLSYIEKKKGRQTTHIVFSFRDITSMTTG"
ORIGIN
        1 gagatatggg ttgatatgta cactgtaaag ccctctggat ggactgtgcg cacgtttgat
       61 aaaccaagga aaagattcat agcctttttc atcgccggca tcctcttcag ggcgataaaa
      121 aaccacttcc ttccccgcga aactcttcaa tgcctgccgt atatccttac tggcttccgc
      181 agaggtcaat ccgaatattt cagcatattt agcaacatgg atctcgcaga taccgtcatg
      241 ttcctgtagg gtgccatcag attttctgat ctggtcaacg aacagataca gcatacgttt
      301 ttgatcccgg gagagactat atgccgcctc agtgaggtcg tttgactgga cgattcgcgg
      361 gctattttta cgtttcttgt gattgataac cgctgtttcc gccatgacag atccatgtga
      421 agtgtgacaa gtttttagat tgtcacacta aataaaaaag agtcaataag cagggataac
      481 tttgtgaaaa aacagcttct tctgagggca atttgtcaca gggttaaggg caatttgtca
      541 cagacaggac tgtcatttga gggtgatttg tcacactgaa agggcaattt gtcacaacac
      601 cttctctaga accagcatgg ataaaggcct acaaggcgct ctaaaaaaga agatctaaaa
      661 actataaaaa aaataattat aaaaatatcc ccgtggataa gtggataacc ccaagggaag
      721 ttttttcagg catcgtgtgt aagcagaata tataagtgct gttccctggt gcttcctcgc
      781 tcactcgacc gggagggttc gagaaggggg ggcacccccc ttcggcgtgc gcggtcacgc
      841 gcacagggcg cagccctggt taaaaacaag gtttataaat attggtttaa aagcaggtta
      901 aaagacaggt tagcggtggc cgaaaaacgg gcggaaaccc ttgcaaatgc tggattttct
      961 gcctgtggac agcccctcaa atgtcaatag gtgcgcccct catctgtcag cactctgccc
     1021 ctcaagtgtc aaggatcgcg cccctcatct gtcagtagtc gcgcccctca agtgtcaata
     1081 ccgcagggca cttatcccca ggcttgtcca catcatctgt gggaaactcg cgtaaaatca
     1141 ggcgttttcg ccgatttgcg aggctggcca gctccacgtc gccggccgaa atcgagcctg
     1201 cccctcatct gtcaacgccg cgccgggtga gtcggcccct caagtgtcaa cgtccgcccc
     1261 tcatctgtca gtgagggcca agttttccgc gaggtatcca caacgccggc ggccggccgc
     1321 ggtgtctcgc acacggcttc gacggcgttt ctggcgcgtt tgcagggcca tagacggccg
     1381 ccagcccagc ggcgagggca accagccgag ggcttcgccc tgtcgctcga ctgcggcgag
     1441 cactactggc tgtaaaagga cagaccacat catggttctg tgttcattag gttgttctgt
     1501 ccattgctga cataatccgc tccacttcaa cgtaacaccg cacgaagatt tctattgttc
     1561 ctgaaggcat attcaaatcg ttttcgttac cgcttgcagg catcatgaca gaacactact
     1621 tcctataaac gctacacagg ctcctgagat taataatgcg gatctctacg ataatgggag
     1681 attttcccga ctgtttcgtt cgcttctcag tggataacag ccagcttctc tgtttaacag
     1741 acaaaaacag catatccact cagttccaca tttccatata aaggccaagg catttattct
     1801 caggataatt gtttcagcat cgcaaccgca tcagactccg gcatcgcaaa ctgcacccgg
     1861 tgccgggcag ccacatccag cgcaaaaacc ttcgtgtaga cttccgttga actgatggac
     1921 ttatgtccca tcaggctttg cagaactttc agcggtatac cggcatacag catgtgcatc
     1981 gcataggaat ggcggaacgt atgtggtgtg accggaacag agaacgtcac accgtcagca
     2041 gcagcggcgg caaccgcctc cccaatccag gtcctgaccg ttctgtccgt cacttcccag
     2101 atccgcgctt tctctgtcct tcctgtgcga cggttacgcc gctccatgag cttatcgcga
     2161 ataaatacct gtgacggaag atcacttcgc agaataaata aatcctggtg tccctgttga
     2221 taccgggaag ccctgggcca acttttggcg aaaatgagac gttgatcggc acgtaagagg
     2281 ttccaacttt caccataatg aaataagatc actaccgggc gtattttttg agttatcgag
     2341 attttcagga gctaaggaag ctaaaatgga gaaaaaaatc actggatata ccaccgttga
     2401 tatatcccaa tggcatcgta aagaacattt tgaggcattt cagtcagttg ctcaatgtac
     2461 ctataaccag accgttcagc tggatattac ggccttttta aagaccgtaa agaaaaataa
     2521 gcacaagttt tatccggcct ttattcacat tcttgcccgc ctgatgaatg ctcatccgga
     2581 atttcgtatg gcaatgaaag acggtgagct ggtgatatgg gatagtgttc acccttgtta
     2641 caccgttttc catgagcaaa ctgaaacgtt ttcatcgctc tggagtgaat accacgacga
     2701 tttccggcag tttctacaca tatattcgca agatgtggcg tgttacggtg aaaacctggc
     2761 ctatttccct aaagggttta ttgagaatat gtttttcgtc tcagccaatc cctgggtgag
     2821 tttcaccagt tttgatttaa acgtggccaa tatggacaac ttcttcgccc ccgttttcac
     2881 catgggcaaa tattatacgc aaggcgacaa ggtgctgatg ccgctggcga ttcaggttca
     2941 tcatgccgtt tgtgatggct tccatgtcgg cagaatgctt aatgaattac aacagtactg
     3001 cgatgagtgg cagggcgggg cgtaattttt ttaaggcagt tattggtgcc cttaaacgcc
     3061 tggttgctac gcctgaataa gtgataataa gcggatgaat ggcagaaatt cgatgataag
     3121 ctgtcaaaca tgagaattgg tcgacggccc gggcggccgc aaggagttcg cgttggccga
     3181 ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg
     3241 caattaatgt gagttagctc actcattagg caccccaggc tttacacttt atgcttccgg
     3301 ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca cacaggaaac agctatgacc
     3361 atgattacgc caagctattt aggtgagact atagaatact caagcttgca tgcctgcagg
     3421 tcgactctag aggatcccac attattgaag catttatcag ggttattgtc tcatgagcgg
     3481 atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg
     3541 aaaagtgcca cctgacgtgt cgacgcggcc gctagcgatg accctgctga ttggttcgct
     3601 gaccatttcc gggtgcggga cggcgttacc agaaactcag aaggttcgtc caaccaaacc
     3661 gactctgacg gcagtttacg agagagatga tagggtctgc ttcagtaagc cagatgctac
     3721 acaattaggc ttgtacatat tgtcgttaga acgcggctac aattaataca taaccttatg
     3781 tatcatacac atacgattta ggtgacacta tagagtaaat cctgtgtgct aattgaggtg
     3841 cattggtctg caaatcgagt tgctaggcaa taaacacatt tggattaatt ttaatcgttc
     3901 gttgagcgat tagcagagaa ctgaccagaa catgtctggt cgtaaagctc agggaaaaac
     3961 cctgggcgtc aatatggtac gacgaggagt tcgctccttg tcaaacgagc tcgtgagcaa
     4021 gggcgaggca gtgatcaagg agttcatgcg gttcaaggtg cacatggagg gctccatgaa
     4081 cggccacgag ttcgagatcg agggcgaggg cgagggccgc ccctacgagg gcacccagac
     4141 cgccaagctg aaggtgacca agggtggccc cctgcccttc tcctgggaca tcctgtcccc
     4201 tcagttcatg tacggctcca gggccttcac caagcacccc gccgacatcc ccgactacta
     4261 taagcagtcc ttccccgagg gcttcaagtg ggagcgcgtg atgaacttcg aggacggcgg
     4321 cgccgtgacc gtgacccagg acacctccct ggaggacggc accctgatct acaaggtgaa
     4381 gctccgcggc accaacttcc ctcctgacgg ccccgtaatg cagaagaaga caatgggctg
     4441 ggaagcgtcc accgagcggt tgtaccccga ggacggcgtg ctgaagggcg acattaagat
     4501 ggccctgcgc ctgaaggacg gcgggcgcta cctggcggac ttcaagacca cctacaaggc
     4561 caagaagccc gtgcagatgc ccggcgccta caacgtggac cgcaagttgg acatcacctc
     4621 ccacaacgag gactacaccg tggtggaaca gtacgaacgc tccgagggcc gccactccac
     4681 cggcggcatg gacgagctgt acaagaccgg taactttgac cttctcaagt tggccggcga
     4741 cgtcgagtcc aacccagggc ccctgcagca aattttcgtg aagaccctga cgggcaagac
     4801 catcactctt gaggtcgagc ccagtgacac catcgagaat gtcaaggcca agatccaaga
     4861 caaggaaggc atcccacctg accagcagag gctgatattc gcgggcaaac agctggagga
     4921 tggccgcacc ctgtccgact acaacatcca gaaagagtcc accttgcacc tggtgctgtg
     4981 tctccgcggt ggaatgtccg gtcgtaaagc ccagggcaaa accctaggcg taaatatggt
     5041 ccgacgcgga gtccgcaaca tgacaatgtc catgagcatg atcttggtag gagtgatcat
     5101 gatgtttttg tctctaggag ttggggcgga tcaaggatgc gccatcaact ttggcaagag
     5161 agagctcaag tgcggagatg gtatcttcat atttagagac tctgatgact ggctgaacaa
     5221 gtactcatac tatccagaag atcctgtgaa gcttgcatca atagtgaaag cctcttttga
     5281 agaagggaag tgtggcctaa attcagttga ctcccttgag catgagatgt ggagaagcag
     5341 ggcagatgag atcaatgcca tttttgagga aaacgaggtg gacatttctg ttgtcgtgca
     5401 ggatccaaag aatgtttacc agagaggaac tcatccattt tccagaattc gggatggtct
     5461 gcagtatggt tggaagactt ggggtaagaa ccttgtgttc tccccaggga ggaagaatgg
     5521 aagcttcatc atagatggaa agtccaggaa agaatgcccg ttttcaaacc gggtctggaa
     5581 ttctttccag atagaggagt ttgggacggg agtgttcacc acacgcgtgt acatggacgc
     5641 agtctttgaa tacaccatag actgcgatgg atctatcttg ggtgcagcgg tgaacggaaa
     5701 aaagagtgcc catggctctc caacattttg gatgggaagt catgaagtaa atgggacatg
     5761 gatgatccac accttggagg cattagatta caaggagtgt gagtggccac tgacacatac
     5821 gattggaaca tcagttgaag agagtgaaat gttcatgccg agatcaatcg gaggcccagt
     5881 tagctctcac aatcatatcc ctggatacaa ggttcagacg aacggacctt ggatgcaggt
     5941 accactagaa gtgaagagag aagcttgccc agggactagc gtgatcattg atggcaactg
     6001 tgatggacgg ggaaaatcaa ccagatccac cacggatagc gggaaagtta ttcctgaatg
     6061 gtgttgccgc tcctgcacaa tgccgcctgt gagcttccat ggtagtgatg ggtgttggta
     6121 tcccatggaa attaggccaa ggaaaacgca tgaaagccat ctggtgcgct cctgggttac
     6181 agctggagaa atacatgctg tcccttttgg tttggtgagc atgatgatag caatggaagt
     6241 ggtcctaagg aaaagacagg gaccaaagca aatgttggtt ggaggagtag tgctcttggg
     6301 agcaatgctg gtcgggcaag taactctcct tgatttgctg aaactcacag tggctgtggg
     6361 attgcatttc catgagatga acaatggagg agacgccatg tatatggcgt tgattgctgc
     6421 cttttcaatc agaccagggc tgctcatcgg ctttgggctc aggaccctat ggagccctcg
     6481 ggaacgcctt gtgctgaccc taggagcagc catggtggag attgccttgg gtggcgtgat
     6541 gggcggcctg tggaagtatc taaatgcagt ttctctctgc atcctgacaa taaatgctgt
     6601 tgcttctagg aaagcatcaa ataccatctt gcccctcatg gctctgttga cacctgtcac
     6661 tatggctgag gtgagacttg ccgcaatgtt cttttgtgcc atggttatca taggggtcct
     6721 tcaccagaat ttcaaggaca cctccatgca gaagactata cctctggtgg ccctcacact
     6781 cacatcttac ctgggcttga cacaaccttt tttgggcctg tgtgcatttc tggcaacccg
     6841 catatttggg cgaaggagta tcccagtgaa tgaggcactc gcagcagctg gtctagtggg
     6901 agtgctggca ggactggctt ttcaggagat ggagaacttc cttggtccga ttgcagttgg
     6961 aggactcctg atgatgctgg ttagcgtggc tgggagggtg gatgggctag agctcaagaa
     7021 gcttggtgaa gtttcatggg aagaggaggc ggagatcagc gggagttccg cccgctatga
     7081 tgtggcactc agtgaacaag gggagttcaa gctgctttct gaagagaaag tgccatggga
     7141 ccaggttgtg atgacctcgc tggccttggt tggggctgcc ctccatccat ttgctcttct
     7201 gctggtcctt gctgggtggc tgtttcatgt caggggagcg cgcagaagtg gtcaccggag
     7261 aagtggggat gtcttgtggg atattcccac tcctaagatc atcgaggaat gtgaacatct
     7321 ggaggatggg atttatggca tattccagtc aaccttcttg ggggcctccc agcgaggagt
     7381 gggagtggca cagggagggg tgttccacac aatgtggcat gtcacaagag gagctttcct
     7441 tgtcaggaat ggcaagaagt tgattccatc ttgggcttca gtaaaggaag accttgtcgc
     7501 ctatggtggc tcatggaagt tggaaggcag atgggatgga gaggaagagg tccagttgat
     7561 cgcggctgtt ccaggaaaga acgtggtcaa cgtccagaca aaaccgagct tgttcaaagt
     7621 gaggaatggg ggagaaatcg gggctgtcgc tcttgactat ccgagtggca cttcaggatc
     7681 tcctattgtt aacaggaacg gagaggtgat tgggctgtac ggcaatggca tccttgtcgg
     7741 tgacaactcc ttcgtgtccg ccatatccca gactgaggtg aaggaagaag gaaaggagga
     7801 gctccaagag atcccgacaa tgctaaagaa aggaatgaca actgtccttg attttcatcc
     7861 tggagctggg aagacaagac gtttcctccc acagatcttg gccgagtgcg cacggagacg
     7921 cttgcgcact cttgtgttgg cccccaccag ggttgttctt tctgaaatga aggaggcttt
     7981 tcacggcctg gacgtgaaat tccacacaca ggctttttcc gctcacggca gcgggagaga
     8041 agtcattgat gccatgtgcc atgccaccct aacttacagg atgttggaac caactagggt
     8101 tgttaactgg gaagtgatca ttatggatga agcccatttt ttggatccag ctagcatagc
     8161 cgctagaggt tgggcagcgc acagagctag ggcaaatgaa agtgcaacaa tcttgatgac
     8221 agccacaccg cctgggacta gtgatgaatt tccacattca aatggtgaaa tagaagatgt
     8281 tcaaacggac atacccagtg agccctggaa cacagggcat gactggatcc tggctgacaa
     8341 aaggcccacg gcatggttcc ttccatccat cagagctgca aatgtcatgg ctgcctcttt
     8401 gcgtaaggct ggaaagagtg tggtggtcct gaacaggaaa acctttgaga gagaataccc
     8461 cacgataaag cagaagaaac ctgactttat attggccact gacatagctg aaatgggagc
     8521 caacctttgc gtggagcgag tgctggattg caggacggct tttaagcctg tgcttgtgga
     8581 tgaagggagg aaggtggcaa taaaagggcc acttcgtatc tccgcatcct ctgctgctca
     8641 aaggaggggg cgcattggga gaaatcccaa cagagatgga gactcatact actattctga
     8701 gcctacaagt gaaaataatg cccaccacgt ctgctggttg gaggcctcaa tgctcttgga
     8761 caacatggag gtgaggggtg gaatggtcgc cccactctat ggcgttgaag gaactaaaac
     8821 accagtttcc cctggtgaaa tgagactgag ggatgaccag aggaaagtct tcagagaact
     8881 agtgaggaat tgtgacctgc ccgtttggct ttcgtggcaa gtggccaagg ctggtttgaa
     8941 gacgaatgat cgtaagtggt gttttgaagg ccctgaggaa catgagatct tgaatgacag
     9001 cggtgaaaca gtgaagtgca gggctcctgg aggagcaaag aagcctctgc gcccaaggtg
     9061 gtgtgatgaa agggtgtcat ctgaccagag tgcgctgtct gaatttatta agtttgctga
     9121 aggtaggagg ggagctgctg aagtgctagt tgtgctgagt gaactccctg atttcctggc
     9181 taaaaaaggt ggagaggcaa tggataccat cagtgtgttc ctccactctg aggaaggctc
     9241 tagggcttac cgcaatgcac tatcaatgat gcctgaggca atgacaatag tcatgctgtt
     9301 tatactggct ggactactga catcgggaat ggtcatcttt ttcatgtctc ccaaaggcat
     9361 cagtagaatg tctatggcga tgggcacaat ggccggctgt ggatatctca tgttccttgg
     9421 aggcgtcaaa cccactcaca tctcctatgt catgctcata ttctttgtcc tgatggtggt
     9481 tgtgatcccc gagccagggc aacaaaggtc catccaagac aaccaagtgg catacctcat
     9541 tattggcatc ctgacgctgg tttcagcggt ggcagccaac gagctaggca tgctggagaa
     9601 aaccaaagag gacctctttg ggaagaagaa cttaattcca tctagtgctt caccctggag
     9661 ttggccggat cttgacctga agccaggagc tgcctggaca gtgtacgttg gcattgttac
     9721 aatgctctct ccaatgttgc accactggat caaagtcgaa tatggcaacc tgtctctgtc
     9781 tggaatagcc cagtcagcct cagtcctttc tttcatggac aaggggatac cattcatgaa
     9841 gatgaatatc tcggtcataa tgctgctggt cagtggctgg aattcaataa cagtgatgcc
     9901 tctgctctgt ggcatagggt gcgccatgct ccactggtct ctcattttac ctggaatcaa
     9961 agcgcagcag tcaaagcttg cacagagaag ggtgttccat ggcgttgcca agaaccctgt
    10021 ggttgatggg aatccaacag ttgacattga ggaagctcct gaaatgcctg ccctttatga
    10081 gaagaaactg gctctatatc tccttcttgc tctcagccta gcttctgttg ccatgtgcag
    10141 aacgcccttt tcattggctg aaggcattgt cctagcatca gctgccttag ggccgctcat
    10201 agagggaaac accagccttc tttggaatgg acccatggct gtctccatga caggagtcat
    10261 gagggggaat cactatgctt ttgtgggagt catgtacaat ctatggaaga tgaaaactgg
    10321 acgccggggg agcgcgaatg gaaaaacttt gggtgaagtc tggaagaggg aactgaatct
    10381 gttggacaag cgacagtttg agttgtataa aaggaccgac attgtggagg tggatcgtga
    10441 tacggcacgc aggcatttgg ccgaagggaa ggtggacacc ggggtggcgg tctccagggg
    10501 gaccgcaaag ttaaggtggt tccatgagcg tggctatgtc aagctggaag gtagggtgat
    10561 tgacctgggg tgtggccgcg gaggctggtg ttactacgct gctgcgcaaa aggaagtgag
    10621 tggggtcaaa ggatttactc ttggaagaga cggccatgag aaacccatga atgtgcaaag
    10681 tctgggatgg aacatcatca ccttcaagga caaaactgat atccaccgcc tagaaccagt
    10741 gaaatgtgac acccttttgt gtgacattgg agagtcatca tcgtcatcgg tcacagaggg
    10801 ggaaaggacc gtgagagttc ttgatactgt agaaaaatgg ctggcttgtg gggttgacaa
    10861 cttctgtgtg aaggtgttag ctccatacat gccagatgtt cttgagaaac tggaattgct
    10921 ccaaaggagg tttggcggaa cagtgatcag gaaccctctc tccaggaatt ccactcatga
    10981 aatgtactac gtgtctggag cccgcagcaa tgtcacattt actgtgaacc aaacatcccg
    11041 cctcctgatg aggagaatga ggcgtccaac tggaaaagtg accctggagg ctgacgtcat
    11101 cctcccaatt gggacacgca gtgttgagac agacaaggga cccctggaca aagaggccat
    11161 agaagaaagg gttgagagga taaaatctga gtacatgacc tcttggtttt atgacaatga
    11221 caacccctac aggacctggc actactgtgg ctcctatgtc acaaaaacct caggaagtgc
    11281 ggcgagcatg gtaaatggtg ttattaaaat tctgacatat ccatgggaca ggatagagga
    11341 ggtcacaaga atggcaatga ctgacacaac cccttttgga cagcaaagag tgtttaaaga
    11401 aaaagttgac accagagcaa aggatccacc agcgggaact aggaagatca tgaaagttgt
    11461 caacaggtgg ctgttccgcc acctggccag agaaaagaac cccagactgt gcacaaagga
    11521 agaatttatt gcaaaagtcc gaagtcatgc agccattgga gcttacctgg aagaacaaga
    11581 acagtggaag actgccaatg aggctgtcca agacccaaag ttctgggaac tggtggatga
    11641 agaaaggaag ctgcaccaac aaggcaggtg tcggacttgt gtgtacaaca tgatggggaa
    11701 aagagagaag aagctgtcag agtttgggaa agcaaaggga agccgtgcca tatggtatat
    11761 gtggctggga gcgcggtatc ttgagtttga ggccctggga ttcctgaatg aggaccattg
    11821 ggcttccagg gaaaactcag gaggaggagt ggaaggcatt ggcttacaat acctaggata
    11881 tgtgatcaga gacctggctg caatggatgg tggtggattc tacgcggatg acaccgctgg
    11941 atgggacacg cgcatcacag aggcagacct tgatgatgaa caggagatct tgaactacat
    12001 gagcccacat cacaaaaaac tggcacaagc agtgatggaa atgacataca agaacaaagt
    12061 ggtgaaagtg ttgagaccag ccccaggagg gaaagcctac atggatgtca taagtcgacg
    12121 agaccagaga ggatccgggc aggtagtgac ttatgctctg aacaccatca ccaacttgaa
    12181 agtccaattg atcagaatgg cagaagcaga gatggtgata catcaccaac atgttcaaga
    12241 ttgtgatgaa tcagttctga ccaggctgga ggcatggctc actgagcacg gatgtgacag
    12301 actgaagagg atggcggtga gtggagacga ctgtgtggtc cggcccatcg atgacaggtt
    12361 cggcctggcc ctgtcccatc tcaacgccat gtccaaggtt agaaaggaca tatctgaatg
    12421 gcagccatca aaagggtgga atgattggga gaatgtgccc ttctgttccc accacttcca
    12481 tgaactacag ctgaaggatg gcaggaggat tgtggtgcct tgccgagaac aggacgagct
    12541 cattgggaga ggaagggtgt ctccaggaaa cggctggatg atcaaggaaa cagcttgcct
    12601 cagcaaagcc tatgccaaca tgtggtcact gatgtatttt cacaaaaggg acatgaggct
    12661 actgtcattg gctgtttcct cagctgttcc cacctcatgg gttccacaag gacgcacaac
    12721 atggtcgatt catgggaaag gggagtggat gaccacggaa gacatgcttg aggtgtggaa
    12781 cagagtatgg ataaccaaca acccacacat gcaggacaag acaatggtga aaaaatggag
    12841 agatgtccct tatctaacca agagacaaga caagctgtgc ggatcactga ttggaatgac
    12901 caatagggcc acctgggcct cccacatcca tttagtcatc catcgtatcc gaacgctgat
    12961 tggacaggag aaatacactg actacctaac agtcatggac aggtattctg tggatgctga
    13021 cctgcaactg ggtgagctta tctgaaacac catctaacag gaataaccgg gatacaaacc
    13081 acgggtggag aaccggactc cccacaacct gaaaccggga tataaaccac ggctggagaa
    13141 ccggactccg cacttaaaat gaaacagaaa ccgggataaa aactacggat ggagaaccgg
    13201 actccacaca ttgagacaga agaagttgtc agcccagaac cccacacgag ttttgccact
    13261 gctaagctgt gaggcagtgc aggctgggac agccgacctc caggttgcga aaaacctggt
    13321 ttctgggacc tcccacccca gagtaaaaag aacggagcct ccgctaccac cctcccacgt
    13381 ggtggtagaa agacggggtc tagaggttag aggagaccct ccagggaaca aatagtggga
    13441 ccatattgac gccagggaaa gaccggagtg gttctctgct tttcctccag aggtctgtga
    13501 gcacagtttg ctcaagaata agcagacctt tggatgacaa acacaaaacc actcgagcaa
    13561 gacgtttccc gttgaatatg gctcataaca ccccttgtat tactgtttat gtaagcagac
    13621 agttttattg ttcatgatga tatattttta tcttgtgcaa tgtaacatca gagattttga
    13681 gacacaacgt ggctttgttg aataaatcga acttttgctg agttgaagga tcagatcacg
    13741 catcttcccg acaacgcaga ccgttccgtg gcaaagcaaa agttcaaaat caccaactgg
    13801 tccacctaca acaaagctct catcaaccgt ggctccctca ctttctggct ggatgatggg
    13861 gcgattcagg cctggtatga gtcagcaaca ccttcttcac gaggcagacc tcagcgctag
    13921 cggagtgtag tgggatcccc gggtaccgag ctcgaattcg ccctatagtg agtcgtatta
    13981 caattcactg gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg ttacccaact
    14041 taatcgcctt gcagcacatc cccctttcgc cagctggcgt aatagcgaag aggcccgcac
    14101 cgatcgccct tcccaacagt tgcgcagctg aatggcgaat ggcgcctgat gcggtatttt
    14161 ctccttacgc atctgtgcgg tatttcacac cgcatatggt gcactctcag tacaatctgc
    14221 tctgatgccg catagttaag ccagccccga cacccgccaa cacccgctga cgcgaacccc
    14281 ttgcggccgc atcgaatata acttcgtata atgtatgcta tacgaagtta ttagcgatga
    14341 gctcggactt ccattgttca ttccacggac aaaaacagag aaaggaaacg acagaggcca
    14401 aaaagctcgc tttcagcacc tgtcgtttcc tttcttttca gagggtattt taaataaaaa
    14461 cattaagtta tgacgaagaa gaacggaaac gccttaaacc ggaaaatttt cataaatagc
    14521 gaaaacccgc gaggtcgccg ccccgtaacc tgtcggatca ccggaaagga cccgtaaagt
    14581 gataatgatt atcatctaca tatcacaacg tgcgtggagg ccatcaaacc acgtcaaata
    14641 atcaattatg acgcaggtat cgtattaatt gatctgcatc aacttaacgt aaaagcaact
    14701 tcagacaata caaatcagcg acactgaata cggggcaacc tcatgtccga gctcgcgagc
    14761 tcgtcgacag cgacacactt gcatcggatg cagcccggtt aacgtgccgg cacggcctgg
    14821 gtaaccaggt attttgtcca cataaccgtg cgcaaaatgt tgtggataag caggacacag
    14881 cagcaatcca cagcaggcat acaaccgcac accgaggtta ctccgttcta caggttacga
    14941 cgacatgtca atacttgccc ttgacaggca ttgatggaat cgtagtctca cgctgatagt
    15001 ctgatcgaca atacaagtgg gaccgtggtc ccagaccgat aatcagaccg acaacacgag
    15061 tgggatcgtg gtcccagact aataatcaga ccgacgatac gagtgggacc gtggtcccag
    15121 actaataatc agaccgacga tacgagtggg accgtggttc cagactaata atcagaccga
    15181 cgatacgagt gggaccgtgg tcccagacta ataatcagac cgacgatacg agtgggacca
    15241 tggtcccaga ctaataatca gaccgacgat acgagtggga ccgtggtccc agtctgatta
    15301 tcagaccgac gatacgagtg ggaccgtggt cccagactaa taatcagacc gacgatacga
    15361 gtgggaccgt ggtcccagac taataatcag accgacgata cgagtgggac cgtggtccca
    15421 gtctgattat cagaccgacg atacaagtgg aacagtgggc ccagagagaa tattcaggcc
    15481 agttatgctt tctggcctgt aacaaaggac attaagtaaa gacagataaa cgtagactaa
    15541 aacgtggtcg catcagggtg ctggcttttc aagttcctta agaatggcct caattttctc
    15601 tatacactca gttggaacac gagacctgtc caggttaagc accattttat cgcccttata
    15661 caatactgtc gctccaggag caaactgatg tcgtgagctt aaactagttc ttgatgcaga
    15721 tgacgtttta agcacagaag ttaaaagagt gataacttct tcagcttcaa atatcacccc
    15781 agcttttttc tgctcatgaa ggttagatgc ctgctgctta agtaattcct ctttatctgt
    15841 aaaggctttt tgaagtgcat cacctgaccg ggcagatagt tcaccggggt gagaaaaaag
    15901 agcaacaact gatttaggca atttggcggt gttgatacag cgggtaataa tcttacgtga
    15961 aatattttcc gcatcagcca gcgcagaaat atttccagca aattcattct gcaatcggct
    16021 tgcataacgc tgaccacgtt cataagcact tgttgggcga taatcgttac ccaatctgga
    16081 taatgcagcc atctgctcat catccagctc gccaaccaga acacgataat cactttcggt
    16141 aagtgcagca gctttacgac ggcgactccc atcggcaatt tctatgacac cagatactct
    16201 tcgaccgaac gccggtgtct gttgaccagt cagtagaaaa gaagggatga gatcatccag
    16261 tgcgtcctca gtaagcagct cctggtcacg ttcattacct gaccataccc gagaggtctt
    16321 ctcaacacta tcaccccgga gcacttcaag agtaaacttc acatcccgac cacatacagg
    16381 caaagtaatg gcattaccgc gagccattac tcctacgcgc gcaattaacg aatccaccat
    16441 cggggcagct ggtgtcgata acgaagtatc ttcaaccggt tgagtattga gcgtatgttt
    16501 tggaataaca ggcgcacgct tcattatcta atctcccagc gtggtttaat cagacgatcg
    16561 aaaatttcat tgcagacagg ttcccaaata gaaagagcat ttctccaggc accagttgaa
    16621 gagcgttgat caatggcctg ttcaaaaaca gttctcatcc ggatctgacc tttaccaact
    16681 tcatccgttt cacgtacaac attttttaga accatgcttc cccaggcatc ccgaatttgc
    16741 tcctccatcc acggggactg agagccatta ctattgctgt atttggtaag caaaatacgt
    16801 acatcaggct cgaacccttt aagatcaacg ttcttgagca gatcacgaag catatcgaaa
    16861 aactgcagtg cggaggtgta gtcaaacaac tcagcaggcg tgggaacaat cagcacatca
    16921 gcagcacata cgacattaat cgtgccgata cccaggttag gcgcgctgtc aataactatg
    16981 acatcatagt catgagcaac agtttcaatg gccagtcgga gcatcaggtg tggatcggtg
    17041 ggcagtttac cttcatcaaa tttgcccatt aactcagttt caatacggtg cagagccaga
    17101 caggaaggaa taatgtcaag ccccggccag caagtgggct ttattgcata agtgacatcg
    17161 tccttttccc caagatagaa aggcaggaga gtgtcttctg catgaatatg aagatctggt
    17221 acccatccgt gatacattga ggctgttccc tgggggtcgt taccttccac gagcaaaaca
    17281 cgtagcccct tcagagccag atcctgagca agatgaacag aaactgaggt tttgtaaacg
    17341 ccacctttat gggcagcaac cccgatcacc ggtggaaata cgtcttcagc acgtcgcaat
    17401 cgcgtaccaa acacatcacg catatgatta atttgttcaa ttgtataacc aacacgttgc
    17461 tcaacccgtc ctcgaatttc catatccggg tgcggtagtc gccctgcttt ctcggcatct
    17521 ctgatagcct gagaagaaac cccaactaaa tccgctgctt cacctattct ccagcgccgg
    17581 gttattttcc tcgcttccgg gctgtcatca ttaaactgtg caatggcgat agccttcgtc
    17641 atttcatgac cagcgtttat gcactggtta agtgtttcca tgagtttcat tctgaacatc
    17701 ctttaatcat tgctttgcgt ttttttatta aatcttgcaa tttactgcaa agcaacaaca
    17761 aaatcgcaaa gtcatcaaaa aaccgcaaag ttgtttaaaa taagagcaac actacaaaag
    17821 gagataagaa gagcacatac ctcagtcact tattatcact agcgctcgcc gcagccgtgt
    17881 aaccgagcat agcgagcgaa ctggcgagga agcaaagaag aactgttctg tcagatagct
    17941 cttacgctca gcgcaagaag aaatatccac cgtgggaaaa actccaggta gaggtacaca
    18001 cgcggatagc caattcagag taataaactg tgataatcaa ccctcatcaa tgatgacgaa
    18061 ctaacccccg atatcaagtc acatgacgaa gggaaagaga aggaaatcaa ctgtgacaaa
    18121 ctgccctcaa atttggcttc cttaaaaatt acagttcaaa aagtatgaga aaatccatgc
    18181 aggctgaagg aaacagcaaa actgtgacaa attaccctca gtaggtcaga acaaatgtga
    18241 cgaaccaccc tcaaatctgt gacagataac cctcagacta tcctgtcgtc atggaagtga
    18301 tatcgcggaa ggaaaatacg atatgagtcg tctggcggcc tttctttttc tcaatgtatg
    18361 agaggcgcat tggagttctg ctgttgatct cattaacaca gacctgcagg aagcggcggc
    18421 ggaagtcagg catacgctgg taactttgag gcagctggta acgctctatg atccagtcga
    18481 ttttcagaga gacgatgcct gagccatccg gcttacgata ctgacacagg gattcgtata
    18541 aacgcatggc atacggattg gtgatttctt ttgtttcact aagccgaaac tgcgtaaacc
    18601 ggttctgtaa cccgataaag aagggaat
//
