LOCUS       sequence-46380-e         934 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 934)
  AUTHORS   Lian X, Hsiao C, Wilson G, Zhu K, Hazeltine LB, Azarin SM, Raval KK,
            Zhang J, Kamp TJ, Palecek SP
  TITLE     Robust cardiomyocyte differentiation from human pluripotent stem 
            cells via temporal modulation of canonical Wnt signaling.
  JOURNAL   Proc Natl Acad Sci U S A. 2012 May 29.
  PUBMED    22645348
REFERENCE   2  (bases 1 to 934)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2012 May 29."
COMMENT     Sequence Label: pXL002-ishRNA-beta-catenin-1
FEATURES             Location/Qualifiers
     source          1..934
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(363..407)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    592..825
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
ORIGIN
        1 cttataaggt tccctatcag gtgatagaga aaaagcataa cctttcccat catttggatc
       61 caaatgatgg gaaaggttat gctttttttt actagtgtta accgataccg tcgagatccg
      121 ttcactaatc gaatggatct gtctctgtct ctctctccac cttcttcttc tattccttcg
      181 ggcctgtcgg gtcccctcgg ggttgggagg tgggtctgaa acgataatgg tgaatatccc
      241 tgcctaactc tattcactat agaaagtaca gcaaaaacta ttcttaaacc taccaagcct
      301 cctactatca ttatgaataa ttttatatac cacagccaat ttgttatgtt aaaccaattc
      361 cacaaacttg cccatttatc taattccaat aattcttgtt cattcttttc ttgctggttt
      421 tgcgattctt caattaagga gtgtattaag cttgtgtaat tgttaatttc tctgtcccac
      481 tccatccagg tcgtgtgatt ccaaatctgt tccagagatt tattactcca actagcattc
      541 caaggcacag cagtggtgca aatgagtttt ccagagcaac cccaaatccc caggagctgt
      601 tgatccttta ggtatctttc cacagccagg attcttgcct ggagctgctt gatgccccag
      661 actgtgagtt gcaacagatg ctgttgcgcc tcaatagccc tcagcaaatt gttctgctgc
      721 tgcactatac cagacaataa ttgtctggcc tgtaccgtca gcgtcattga cgctgcgccc
      781 atagtgcttc ctgctgctcc caagaaccca aggaacaaag ctcctattcc cactgctctt
      841 ttttctctct gcaccactct tctctttgcc ttggtgggtg ctactcctaa tggttcaatt
      901 tttactactt tatattatat aattcacttc tcca
//
