LOCUS       sequence-463802-        5971 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  AAV vector plasmid expressing human atlastin GTPase 1 (ATL1) fused 
            to myc-tag under the human synapsin (SYN) promoter. It also contains
            an optimised and truncated version of WPRE as a regulatory element..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5971)
  TITLE     Adeno-associated viral vector-mediated delivery of ATL1 in neurons
REFERENCE   2  (bases 1 to 5971)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: AAV-SYN-Myc-ATL1-oPRE
FEATURES             Location/Qualifiers
     source          1..5971
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..130
                     /label=AAV2 ITR
     protein_bind    137..157
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     promoter        179..626
                     /label=hSyn promoter
                     /note="human synapsin I promoter; confers neuron-specific 
                     expression (Kugler et al., 2003)"
     protein_bind    626..650
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      651..660
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             660..689
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     protein_bind    complement(2367..2391)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(3002..3019)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    3008..3215
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     repeat_region   3223..3363
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   3223..3352
                     /label=AAV2 ITR
     rep_origin      3438..3893
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3525..3544)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3735..3756
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(3910..3929)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4029..4051
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(4089..4107)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4175..4279
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4280..4348,4349..5140)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4498..4517)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5311..5899
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5800..5819
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     repeat_region   join(5961..5971,1..130)
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
ORIGIN
        1 ctgcgcgctc gctcgctcac tgaggccgcc cgggcaaagc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct tctagacaac tttgtataga aaagttgctg cagagggccc tgcgtatgag
      181 tgcaagtggg ttttaggacc aggatgaggc ggggtggggg tgcctacctg acgaccgacc
      241 ccgacccact ggacaagcac ccaaccccca ttccccaaat tgcgcatccc ctatcagaga
      301 gggggagggg aaacaggatg cggcgaggcg cgtgcgcact gccagcttca gcaccgcgga
      361 cagtgccttc gcccccgcct ggcggcgcgc gccaccgccg cctcagcact gaaggcgcgc
      421 tgacgtcact cgccggtccc ccgcaaactc cccttcccgg ccaccttggt cgcgtccgcg
      481 ccgccgccgg cccagccgga ccgcaccacg cgaggcgcga gatagggggg cacgggcgcg
      541 accatctgcg ctgcggcgcc ggcgactcag cgctgcctca gtctgcggtg ggcagcggag
      601 gagtcgtgtc gtgcctgaga gcgcagcaag tttgtacaaa aaagcaggct gccaccatgg
      661 aacaaaaact catctcagaa gaggatctga tggccaagaa ccgcagggac agaaacagtt
      721 ggggtggatt ttcggaaaag acatatgaat ggagctcaga agaggaggag ccagtgaaaa
      781 aggcaggacc agtccaagtc ctcattgtca aagatgacca ttcctttgag ttagatgaaa
      841 ctgcattaaa tcggatcctt ctctcggagg ctgtcagaga caaggaggtt gttgctgtat
      901 ctgttgctgg agcatttaga aaaggaaaat cattcctgat ggacttcatg ttgagataca
      961 tgtacaacca ggaatcagtt gattgggttg gagactacaa tgaaccattg actggttttt
     1021 catggagagg tggatctgag cgagagacca caggaattca gatatggagt gaaatcttcc
     1081 ttatcaataa acctgatggt aaaaaggttg cagtgttatt gatggatact cagggaacct
     1141 ttgatagtca gtcaactttg agagattcag ccacagtatt tgcccttagc acaatgatca
     1201 gctcaataca ggtatataac ttatcccaaa atgtccagga ggatgatctt cagcacctcc
     1261 agcttttcac tgagtatggc agactggcaa tggaggaaac attcctgaag ccatttcaga
     1321 gtctgatatt tcttgttcga gactggagtt tcccatacga attttcatat ggagccgatg
     1381 gtggtgccaa attcttggaa aaacgcctca aggtctcagg gaaccagcat gaagaactac
     1441 agaacgtcag aaaacacatc cattcctgtt tcaccaacat ttcctgtttt ctgctacctc
     1501 atcctggctt aaaagtagct accaatccaa actttgatgg aaaattgaaa gaaatagatg
     1561 atgaattcat caaaaacttg aaaatactga ttccttggct acttagtccc gagagcctag
     1621 atattaaaga gatcaatggg aataaaatca cctgccgggg tctggtggag tacttcaagg
     1681 cttatataaa gatctatcaa ggtgaagaat taccacatcc caaatccatg ttacaggcca
     1741 cagcagaagc taacaattta gcagccgtgg caactgccaa ggacacatac aacaaaaaaa
     1801 tggaagagat ttgtggtggt gacaaaccat ttctggcccc aaatgacttg cagaccaaac
     1861 acctgcaact taaggaagaa tctgtgaagc tattccgagg ggtgaagaag atgggtgggg
     1921 aagaatttag ccggcgttac ctgcagcagt tggagagtga aatagatgaa ctttacatcc
     1981 aatatatcaa gcacaatgat agcaaaaata tcttccatgc agctcgtacc ccagccacac
     2041 tgtttgtagt catctttatc acatatgtga ttgctggtgt gactggattc attggtttgg
     2101 acatcatagc tagcctatgc aatatgataa tgggactgac ccttatcacc ctgtgcactt
     2161 gggcatatat ccggtactct ggagaatacc gagagctggg agctgtaata gaccaggtgg
     2221 ctgcagctct gtgggaccag ggaagtacaa atgaggcttt gtacaagctt tacagtgcag
     2281 cagcaaccca cagacatctg tatcatcaag ctttccctac accaaagtcg gaatctactg
     2341 aacaatcaga aaagaaaaaa atgtaaaccc agctttcttg tacaaagtgg gaattcgagc
     2401 atcttaccgc catttatacc catatttgtt ctgtttttct tgatttgggt atacatttaa
     2461 atgttaataa aacaaaatgg tggggcaatc atttacattt ttagggatat gtaattacta
     2521 gttcaggtgt attgccacaa gacaaacatg ttaagaaact ttcccgttat ttacgctctg
     2581 ttcctgttaa tcaacctctg gattacaaaa tttgtgaaag attgactgat attcttaact
     2641 atgttgctcc ttttacgctg tgtggatatg ctgctttata gcctctgtat ctagctattg
     2701 cttcccgtac ggctttcgtt ttctcctcct tgtataaatc ctggttgctg tctcttttag
     2761 aggagttgtg gcccgttgtc cgtcaacgtg gcgtggtgtg ctctgtgttt gctgacgcaa
     2821 cccccactgg ctggggcatt gccaccacct gtcaactcct ttctgggact ttcgctttcc
     2881 ccctcccgat cgccacggca gaactcatcg ccgcctgcct tgcccgctgc tggacagggg
     2941 ctaggttgct gggcactgat aattccgtgg tgttgtcgaa ttcctagagc tcgctgatca
     3001 gcctcgactg tgccttctag ttgccagcca tctgttgttt gcccctcccc cgtgccttcc
     3061 ttgaccctgg aaggtgccac tcccactgtc ctttcctaat aaaatgagga aattgcatcg
     3121 cattgtctga gtaggtgtca ttctattctg gggggtgggg tggggcagga cagcaagggg
     3181 gaggattggg aagagaatag caggcatgct ggggagggcc gcaggaaccc ctagtgatgg
     3241 agttggccac tccctctctg cgcgctcgct cgctcactga ggccgggcga ccaaaggtcg
     3301 cccgacgccc gggctttgcc cgggcggcct cagtgagcga gcgagcgcgc agctgcctgc
     3361 aggggcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatac
     3421 gtcaaagcaa ccatagtacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt
     3481 tacgcgcagc gtgaccgcta cacttgccag cgccttagcg cccgctcctt tcgctttctt
     3541 cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc
     3601 tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg atttgggtga
     3661 tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc
     3721 cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaact ctatctcggg
     3781 ctattctttt gatttataag ggattttgcc gatttcggtc tattggttaa aaaatgagct
     3841 gatttaacaa aaatttaacg cgaattttaa caaaatatta acgtttacaa ttttatggtg
     3901 cactctcagt acaatctgct ctgatgccgc atagttaagc cagccccgac acccgccaac
     3961 acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt
     4021 gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag
     4081 acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc
     4141 ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt
     4201 ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata
     4261 atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt
     4321 tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc
     4381 tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat
     4441 ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct
     4501 atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca
     4561 ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg
     4621 catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa
     4681 cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg
     4741 ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga
     4801 cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg
     4861 cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt
     4921 tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg
     4981 agccggtgag cgtggaagcc gcggtatcat tgcagcactg gggccagatg gtaagccctc
     5041 ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca
     5101 gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc
     5161 atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat
     5221 cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc
     5281 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg
     5341 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct
     5401 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct
     5461 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct
     5521 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg
     5581 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
     5641 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
     5701 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
     5761 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
     5821 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
     5881 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
     5941 ctggcctttt gctcacatgt cctgcaggca g
//
