LOCUS       sequence-463804-       20395 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses Eggerthella lenta A2 Dadh (Delta-Elen_0481) in 
            Gordonibacter urolithinfaciens DSM 27213.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 20395)
  AUTHORS   Dong X, Bae M, Le C, Aguilar Ramos MA, Balskus EP
  TITLE     Enantiocomplementary Gut Bacterial Enzymes Metabolize Dietary 
            Polyphenols.
  JOURNAL   J Am Chem Soc. 2025 Mar 5;147(9):7231-7244. doi: 
            10.1021/jacs.4c09892. Epub 2025 Feb 24.
  PUBMED    39993729
REFERENCE   2  (bases 1 to 20395)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/jacs.4c09892"; journalName: "J Am Chem Soc"; date: 
            "2025-03-5- 5"; volume: "147"; issue: "9"; pages: "7231-7244"
COMMENT     Sequence Label: pXD80ENSF7.9-Dadh-Delta-Elen0481(ElA2)
FEATURES             Location/Qualifiers
     source          1..20395
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(19..813)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     primer_bind     1300..1318
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     misc_feature    1475..1494
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that start with ""ATG"""
     CDS             complement(3005..3028)
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
     terminator      14019..14046
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     terminator      14178..14205
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     CDS             complement(14637..15488)
                     /codon_start=1
                     /gene="repC"
                     /product="replication protein C of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepC
                     /translation="MVKPKNKHSLSHVRHDPAHCLAPGLFRALKRGERKRSKLDVTYDY
                     GDGKRIEFSGPEPLGADDLRILQGLVAMAGPNGLVLGPEPKTEGGRQLRLFLEPKWEAV
                     TADAMVVKGSYRALAKEIGAEVDSGGALKHIQDCIERLWKVSIIAQNGRKRQGFRLLSE
                     YASDEADGRLYVALNPLIAQAVMGGGQHVRISMDEVRALDSETARLLHQRLCGWIDPGK
                     TGKASIDTLCGYVWPSEASGSTMRKRRQRVREALPELVALGWTVTEFAAGKYDITRPKA
                     AG"
     CDS             complement(15475..16314)
                     /codon_start=1
                     /gene="repA"
                     /product="replication protein A of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepA
                     /translation="MATHKPINILEAFAAAPPPLDYVLPNMVAGTVGALVSPGGAGKSM
                     LALQLAAQIAGGPDLLEVGELPTGPVIYLPAEDPPTAIHHRLHALGAHLSAEERQAVAD
                     GLLIQPLIGSLPNIMAPEWFDGLKRAAEGRRLMVLDTLRRFHIEEENASGPMAQVIGRM
                     EAIAADTGCSIVFLHHASKGAAMMGAGDQQQASRGSSVLVDNIRWQSYLSSMTSAEAEE
                     WGVDDDQRRFFVRFGVSKANYGAPFADRWFRRHDGGVLKPAVLERQRKSKGVPRGEA"
     CDS             complement(16825..17796)
                     /codon_start=1
                     /gene="repB"
                     /product="replication protein B of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepB
                     /translation="MKNDRTLQAIGRQLKAMGCERFDIGVRDATTGQMMNREWSAAEVL
                     QNTPWLKRMNAQGNDVYIRPAEQERHGLVLVDDLSEFDLDDMKAEGREPALVVETSPKN
                     YQAWVKVADAAGGELRGQIARTLASEYDADPASADSRHYGRLAGFTNRKDKHTTRAGYQ
                     PWVLLRESKGKTATAGPALVQQAGQQIEQAQRQQEKARRLASLELSERQLSRHRRTALD
                     EYRSEMAGLVKRFGDDLSKCDFIAAQKLASRGRSAEEIGKAMAEASPALAERKPGHEAD
                     YIERTVSKVMGLPSVQLARAELARAPAPRQRGMDRGGPDFSM"
     oriT            19035..19122
                     /label=RSF1010 oriT
                     /note="origin of transfer of the broad-host-range plasmid 
                     RSF1010 (Scholz et al., 1989)"
     rep_origin      19464..19858
                     /label=RSF1010 oriV
                     /note="replication origin of the broad-host-range plasmid 
                     RSF1010; requires the RSF1010 RepA/B/C proteins for 
                     replication (Scholz et al., 1989)"
     terminator      complement(20021..20064)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     oriT            20160..20269
                     /note="incP origin of transfer"
ORIGIN
        1 ggcgggctgg ccggccttct aaaacaattc atccagtaaa atataatatt ttattttctc
       61 ccaatcaggc ttgatcccca gtaagtcaaa aaatagctcg acatactgtt cttccccgat
      121 atcctccctg atcgaccgga cgcagaaggc aatgtcatac cacttgtccg ccctgccgct
      181 tctcccaaga tcaataaagc cacttacttt gccatctttc acaaagatgt tgctgtctcc
      241 caggtcgccg tgggaaaaga caagttcctc ttcgggcttt tccgtcttta aaaaatcata
      301 cagctcgcgc ggatctttaa atggagtgtc ttcttcccag ttttcgcaat ccacatcggc
      361 cagatcgtta ttcagtaagt aatccaattc ggctaagcgg ctgtctaagc tattcgtata
      421 gggacaatcc gatatgtcga tggagtgaaa gagcctgatg cactccgcat acagctcgat
      481 aatcttttca gggctttgtt catcttcata ctcttccgag caaaggacgc catcggcctc
      541 actcatgagc agattgctcc agccatcatg ccgttcaaag tgcaggacct ttggaacagg
      601 cagctttcct tccagccata gcatcatgtc cttttcccgt tccacatcat aggtggtccc
      661 tttataccgg ctgtccgtca tttttaaata taggttttca ttttctccca ccagcttata
      721 taccttagca ggagacattc cttccgtatc ttttacgcag cggtattttt cgatcagttt
      781 tttcaattcc ggtgatattc tcattttagc catgatgttt aactttgttt tagggcgact
      841 gccctgctgc gtaacatcgt tgctgctcca taacatcaaa catcgaccca cggcgtaacg
      901 cgcttgctgc ttggatgccc gaggcataga ctgtacccca aaaaaacagt cataacaagc
      961 catgaaaacc gccactgcgc cgttaccacc gctgcgttcg gtcaaggttc tggaccagtt
     1021 gcgtgagcgc atacgctact tgcattacag cttacgaacc gaacaggctt atgtccactg
     1081 ggttcgtgcc ttcatccgtt tccacggtgt gcgtcacccg gcaaccttgg gcagcagcga
     1141 agtcgaggca tttctgtcct ggctggcgaa cgagcgcaag gtttcggtct ccacgcattc
     1201 gaaaccggta tcccgccgcc gccgccggat accggtttcg aattgagatt gccacctgac
     1261 gtctaagaaa ccattattat catgacatta acctataaaa ataggcgtat cacgaggccc
     1321 tttcgtcttc aaggcgaaaa aaccccgccg aagcggggtt ttttgcgaaa tataatgacc
     1381 ctcttgataa cccaagaggg cattttttag gaacggttat ttgattatta atccggcggc
     1441 aactgaaacg attcggatcc tgtattacta ttctgaattc gcggccgctt ctagatttag
     1501 tacatcagga ccactgggta gcccagctgg tagatcagca cgcgaaacat cacggaggtg
     1561 gccagcgcgc cgatggcggc gacgaccatg agcgtcttgg aggcgcccac cttcttcttc
     1621 gccacgaagg cgcaggccgc agccacgatc acggagacga tggagcccca gaacatgggc
     1681 gctcccgcgc cggacaccat gacggtcatc aggctgtcga cgtgcgtggg ggccgtggtc
     1741 atcgatgtcg ggtcggcgta gtagccgaat tgcgcgatct tcgccatggc gcaggcgatg
     1801 acgaacacca cgtcggccac gatctgcacg agggccgcga tgaacgtcag ctggatgccc
     1861 gtcgcctcga cggcctcgtc cttcttcagg atggagatca gccagacggc cgcggcgccc
     1921 aggaggcacg cgttgcccag atagaacgcc acgagcgcca ggccccacgc gggacgggcc
     1981 ggcatgaggt aggagtgcgc ggtggcgacc atcatgagca cggcaaccgc gagggtcgcc
     2041 caggccagcg ccttgggaac gggcttgccg cctcgaagga ccacgaacca tgcgacgatg
     2101 acgatggcca gcgccacgca tccgatgagc tcctgggtga tgcccgacgt gatgtgcccg
     2161 aagccgttga agatgcgctc ccagtgctcg aggtgcagga aggctccgat gccgcctacc
     2221 accagcgaga cggcggacgt gatgagcgcg gtcatctgca ggttcctgcc ctggcccttg
     2281 agcgccagga tggacatgcc gcccagcgtg ccgcaggtca agcatacgaa cagcgtgaag
     2341 atgacgagag gccactgcgt ttccattact catcacctcc ttcccagtga tggtcgcgta
     2401 ggatgtagga gtacgacggc ttgttgccca cgtcgggcag cttgtgcacg tcggcgtcgg
     2461 tgaacggctc caggaactcc accatcttgc ggcgcttgcc cagcgggtcc tcggagccct
     2521 cgaacgactc gtagccggcc tcggtgtcgc cgatccagcg ggcgttgccg ccgcactggc
     2581 tgacgcactg gggcagctcg ccctgcgaca gcttctgctc gcacagcgtg cacttctcga
     2641 cgaccttctc ctcctcgttg aggtagcgga cgccgtaggg gcaggccatg acgcagaact
     2701 ggcagccgat gcacttctcc ttgtcgatct ggatggagcc gtcctcggcc acgtgcgacg
     2761 cctcggtggg gcacaccttc acgcactcgg ggttctcgca gtgctggcag ctgatgggca
     2821 ggaagtacat gtacacgtcg gggtactgcc ctgatccgcc cgggatgggg ttcggcccca
     2881 cgcgcaccac tttgttccag tagctgccga tgggcacgtt gttgaccgcc ttgcaggcga
     2941 ccgagcacgc caggcagccc acgcaccggt tgaggtcggt gatgatagcc ttcgtcatgg
     3001 ttcagtgatg gtgatggtga tggtgatgct cgagtgcggc ctccctccct tcgtaggtag
     3061 gcagccattc cttcagacgg gggtcgtcgg aggtatggat gatgggcgtg ccggtggagt
     3121 cgcacggcac ggggttgttg aacggcgagt tctcgggcgt ggccttgtag atcttcacct
     3181 ggtaggcgcg caggttggac gtgccggagt gcgggtcctg ggcgtagtgg tcgatcagct
     3241 ggttcacctg ggagaactgc cagccgtggc cggcgtcctt cacctcgggg taccaccacg
     3301 tgtgctcgag gttgatgacg tcttccttca cgccgtagta caggtcagcc acttcgcgga
     3361 tcttgcccca ctcggtttcg atccacaccc agtcgccctg ctcgatgccg tactcggcgg
     3421 ccgtcttcgg gttgatctcc acgcgcggca cgggccacag ctcgcggcac cagggaagct
     3481 ggcgatgctc ggagtggaag tacaccggga tgcgacggcc ggtggtggcg gtcagcgggt
     3541 actccttgat gcggtcgccg tccttcgggc cgtgaggcgg ctcggtgtag gtgggaagcc
     3601 tccactcggg atggtcggga tggtgcgatt ccatgacggt ggaccagatc tcctgcttca
     3661 tcgtcggggt gaaccagccc ggcttccagt cgccgatgcc cttgccggcc gtgtagtcga
     3721 ggcggcccca cacgcggtcg cgtgcgcgca tcgcgccggt ctggtagcgg cggtacgtgc
     3781 cccagttgcg cggctcgatg tccttcgcct gccaccagcc gtgctcctgg aaggccttga
     3841 cgtactcgtc ccacgtcttg tacttgggcg tgacctcggc catcggaacg ccgtggcgct
     3901 cgaacgtggg cttgccgttc tcgacgtgcc acgtggtgta ggtcagctcg tcgtcggtga
     3961 gcagcttgat ggagtcgtcc agctgccagt tgatgtcggg ccactcgttg cccggctcgt
     4021 cggtccactt ccagttcatg cggcgggcga ggtccatgac gatctcggga tcgtacttgg
     4081 cttccgcggg cggctgcacg cacttgaccg tggcgcccat ggcgccggcg gaaccttggc
     4141 tggcgcgcgg cgagttgagc tcgatccaat gggccaccgg caggatgatg tcggcggtgt
     4201 cgttctgcgg cgtgtgccac aggttgaggt ccacgtagaa gtcaagcgag cacagggcct
     4261 cccaggcgcg cgtggtgttc gtctggctca tgaagttgcc ggactcgttc cagagggcgc
     4321 gcaccgggta ggggtcgccc gtgatgacgg cgtcccacag cgaatggctg tcgcaccagt
     4381 actgccacca gcccagaagc gggaacttgt cgatgccgat ctgcttgcgg ttgacttcct
     4441 ccggcggggt ggtggcgccg ggggcccagg cgctgaagcc ctggaggtcg ccgtcgatgg
     4501 ggacgatggt cgggccgcgg ttgccgcccg gggtgtccat gttgccggtg atgcccacga
     4561 ggttgtcgaa ggcgcggcag ttctggatgg cgctgcagaa gtgctcgacc gccagcatgt
     4621 actggatgcc gccgttgccg tagccggtgg acgggtcgat gcgcgtgccg taggccgtgg
     4681 ccgccgcctc gatctcggag gcggggatgc cggtgatctc ggccgccacc tcgggcttgt
     4741 actcggcggc gcgggccttg tagtgctccc acaccggctt gaccttcacg gtcttgccgt
     4801 ccttcagcgt gatctcgaac tcgccttcca gcgcggggtc gatggcgggg tcgaacggca
     4861 tgggatcggg cagccagccc tgcgtctggc ccggcagcag gttgtcctgc gcggcctcgc
     4921 ggccttggcg cgccttctcg gagtcgtagt tctcgccctc ccagaagccg ccggtctcgt
     4981 cgatgacgcc ttcctggtcg gcgttgaacc aggtgaacgc gccgtactcg tgctcgacgc
     5041 cctcggcctt cagcttctcc cagttgttgt cgtacacgag gaacttatag gggctgccgc
     5101 cctccttgat gtcgctttcc ttgagcagcg cggttttcac gtcccagtag gaaccgtcgg
     5161 tgcgcacggt ggggaagccg gagggctcca tgtcctcgca cacgaggaac ggggcgttcg
     5221 tccacttctt cacgtacagc tcgtcgtaga gcttcttctc gatgatgacg ttcgtccagg
     5281 ccagggccag ggcgccgtcg gtgccggggc gcaggtgctg ccagtagtcg gcttccttgc
     5341 ccatgttggc catgcggggg tcgacgctga tgtgcacgtc ggcgcgcgat gccacgtcca
     5401 ccgtggtgcg gcaggagtcg tcgtagttgg acagctcgga cgcgccgccc cactgcacgt
     5461 acactttcgg gcgcgtgatg gtttccatcc acgacatggc gaactgcgac accatggtgg
     5521 tggcgaagtg gcgcgggccc ttgcagatct gccaggcctc aacgttgttc ggcgtttcca
     5581 gcatgttctt caggatggac tccgagtgca tgcaccagat gcgcgacgtg cccacctggc
     5641 aggcgatggc ctcgccgccg tgcttcgcct tgatgtccat gaagttgtcc acgatggact
     5701 gcatggcctc gtcccacgag atgcgctgcc agccgggctc ctcgcccttc gggttcgtgc
     5761 gcttcatggg atagtgcaga cggtcggggt ggtacgccgc ctggatggac gactgcgact
     5821 tcgtgcagca gttgcccatg gactggaacg ctccctcgtc gccctcgacg cgtatggccc
     5881 gcccgttctg gacgatgacc ttgacgccgc actccatctt gccgcagccg cggcagcacg
     5941 ttttcacggc cacggtgtcg ttgccggaca cgctgctgta cgtctcttcg gccagcgcgg
     6001 tgtgcgtcga agcgccgaac gccgcggctg ccgcaccggt gatggcggcg gtcttgacga
     6061 acgtgcgtcg tgacatggtc aggttaccca tactcctcct ccttctttct cctcgttctc
     6121 gtcttgctcg tcttcgttcg tgcgatcggg gaggggggct gccgggtgaa gcctcgggct
     6181 ccgaaccggt gcaaagtccc cgtgtcgctt gggccgacca tacggatgcg agggggtgca
     6241 gagcatcgtc caaaggttgg aaagcaggtt cgaccgcatc ataaattctt atgatcttgt
     6301 gatcatgaaa aattcgatag tgctttgaac tggggaaact ttaaccgtag gtcataaatt
     6361 attctgaaac tagaatagtt tcctcaaaga gggccgctca tcttttccgt ataatcgaac
     6421 ccgtcgacag aggggtcggc gcgagcgacg ttatgtacgg gctcacaggg ggtggggcat
     6481 ggaaaagctt cggagtgcat gggataccgc gttggggacg agcctttccc tcaagcgcat
     6541 cgcgggcgcg gccttgcaat ggctttggat gctcatgatg ttctacagca tcgtgccgta
     6601 cggcttctcg tcggacgtgc gcggctcgct gtacgtcagc ctgatcatat cgctggtggc
     6661 catggtggtg accatgctgt tcgtctccgt catcgtgcgg cgcgagcggg tgagcggcaa
     6721 ccgctccatc gtgctgggat cggccatcat gatgagcgcg ggcagcgtgc tcacgccgtt
     6781 ctccgatcct accacgcccg tcggcatgct ggtgctgggc ctgagcgcgg tcatgaccgg
     6841 cacgggctcg gccgtgctgt tcttgtgctg gatcgagctc gagtcggggc tgggaggccg
     6901 cttggcgctt gtggagcttg cctcgtcgct gtgcatcgcg ttcgtcgtgg ggttcttgct
     6961 gatcgtaggg ccggcgatac ccgtcatcgt gctcatcgtt gtcatgcccg tgctttcggc
     7021 ggcagctctc aggcgcagcg cgcccgatac gccgcagctg ccgcgcgagc ccgagcagcc
     7081 tttgtcgcgc catacggcga ccctgttcgc gaaggcgctt ctgggcgcgg tgctcatcgg
     7141 catgttgatg gggttcttcg acgtggtgtc ggggttcaaa acctacgagg tgcaggacat
     7201 cttcggcacg tacctgtttc tggcgggctt catcggagcg ttggccatct gcctgatagc
     7261 cgtgttcctg cagcgcgaca gcgtgttctt ctcgtaccgc gtctccatgc tgatgctgtg
     7321 cttgggatgc ttgtccacgc cgttcatggc cgacaacaac acgtattcga gcgcgcttat
     7381 cttcggcggc taccactgct tcgtgctggt gctgtgcgtg gtgtgcatcg acgtggcgtc
     7441 cagcttccgc gtgagcccgg cgcggacgat cggcctgggg ttcgtggcgc tgtacggcgg
     7501 cgagaccgca ggccagctgt tcgcccattc gctggaggcg accggggtca gcatgttcga
     7561 cctcacgctg gtcacgctgg tggccgtgtc gctgctgttc atcgcgcatt tgttcctgtt
     7621 cacggagacc gacctcatca aaatcggcat cggcgaggtc agcctgatgg gcacggtgcc
     7681 cgaagaggcc gccgacgacc cggccgaccc ggtggatccg tgcgcgctga tcgcggagcg
     7741 cttcgccctg tcgccgcgcg agaccgacgt gcttccgctg ctgctggagg gccgcaccat
     7801 ctcgcgcatc caggagacgc tgttcatctc ggcgggcacg gtgagcacgc atatccgcca
     7861 catctaccag aagacgggcg tggacaatcg ccaggagctc atcgacctgg cccacgagac
     7921 ggccgcgcag gacgaggcgg tctgaggcga tggagacggc gtatctgcac gagttcgcga
     7981 aggtggccga ttgcggcagc ttcacggccg ccgcgcgcga gctgcatctg acgcagtcga
     8041 cgctgagcaa gcacgtggcc ttgttggagc gcgaattcgg ggccgacctg ttcgtgcgcg
     8101 accgcagcgg cgtgagcctg accgaggcgg gcggcgtgct gtacgcccag gcgcggcaga
     8161 tggagaagct gctgcgcgcc accgagctgc tggtgcgcgc ggcgcgcgac gggcagggtt
     8221 cggcgggcgc gggggttgcg ggcgcgacgg cgggcgcagc ggcgggcggg ggtgccgtcg
     8281 acggctcgcc tgcggccgcg tgcgacacgg cgcttcgctg caagtgccgg ctcgcggccg
     8341 agcgctgcgg gctcgaccgg cgcgagctgg gcgcgctcat cctgtacgtg gaggagcgcg
     8401 gcttcgaggc gatccagggc gagctggcgc tgacgcgcga cgaggtggcc gacgtgctgg
     8461 gcagcgtgta ccgcaagctg ggcgtgggcg acaagcagga ggcgctcgat ttcatccatt
     8521 ccgtttcgga atgatggtag acgcgcccgc ggcggttggg catactgcgt agaagtgaaa
     8581 gcgctatgaa gctggggggc ttcgagcaaa gggcttcatc cgaagaatgg atggagaccg
     8641 aatatgagtg cttggactgc tgcgtccgtc gaagacgcgg cgaacaatct tcttgaagaa
     8701 ctgaatagcg gcgcgatgcg cttgtgcgtg gagcatgacg cgtccgacga cgttgcgccg
     8761 gcgttgctgc gcgcggctcg cgctcgcgtc gaggcggggt cgaacgtgcg cgtcgtgtgc
     8821 gccgacgccg ttgcagctgc gcgctttcgg gcgcttgcgg ccgacgatcc gctgctgggc
     8881 gcgctcgacg tggtatcggt gcgcgagctg gccctctcga ttctaggcga cgcgcgggtc
     8941 ggcgatgccg tgggacgcga cgcgcgcgtg ctggacgaga acgagctggc cgtgctcatg
     9001 gaggacgtga aggtgagcgg cctcaagccg ggccggctgc gcgagatgct caagttcttc
     9061 tacaagagca tcagcgactg cgccgacgat gacgagcgct ggctgatcac cgccgaagag
     9121 cagaccgtgc acgccatcct caccgagaac ctggaggcgc gccgcgcgtt gctgccgtgc
     9181 gaggtttcgt cgctggcgta tcgcggcctt gtgaaagccg gggtcgagcg cgagccgctg
     9241 acgctggtgg cagacgactt cgggtcgctc agcaaggcgt cgcagcgcct cgtgcgccat
     9301 ctggcgacgg acggcctcgt ggcggcgggc cgcacgctgg cctcgtccag ctccgaggag
     9361 ccgtatccgt gcttcgacgg gttccgcgcg cttgccgacg aggccgactg cctggtgacg
     9421 ctggcgtgcg agcggccggc cgcggcgcgc gagtcccgcg cgctggacga cccggcggcc
     9481 gagttcgcgt tcgcggcggc gtccgtggag gagtgcctgg ccgacggctt ccggccaggc
     9541 gacgtggctg tggccgtgcc gaacgccgct tggggcgagc gcatcgccgc cgagctggaa
     9601 gggcgcggca tcgccgtgga gcgcgggttc gacagcggga agatcaaggg cgacccgcgc
     9661 accgagggcc gttacgccga cctcaagctg gccgcgttcc tgcggttgta cctcgatccg
     9721 cacgatttca cgtcgttgcg ctcgtggctg gggttcggcg attggctgct gcgctccgat
     9781 gcgtttttgg agctgatggc gtacgcgcgc gaccatgaga cgaccgtgcc cgaagccgtg
     9841 gcgcagctgc gcacgcagcg cgacgccgat cgctcctcga cgatattcgg gaagttcgac
     9901 ggcccgctcg acgagctgga cgagctgctg cgcgcctgcg aggaagggct gacgcgcgag
     9961 gccgccgtag cgctgttcga agcgcacgga atgccgttgt ccccgcgcca cgtggagctg
    10021 ctgggcgacg accctgcgca tgcggacgtc gagcgcttgg cgcgctgcgg gttcgcgaag
    10081 ccggtggagg atgtcgcgcg cgatgccgtg catgtggtct cctacgcgcg ctgccacggg
    10141 caccatgtgc gcacggtgtt cgtcaccggc ctggtgaacg gcttcttgcc ggcgagcgac
    10201 gccgtggacg acaagttcac catcgaccat cggcgcgtgg cgctcgaacg cgagcgcgcg
    10261 ctgttcctgg acgtgctgtc ctgtgcgagc gaacgagcgg tatgcacgcg gttcgtgcgc
    10321 gaccttttag agaacaccgc gttcacgaag gtgcagacct cgcgcgtgtt cgtgcgcgac
    10381 ggcgagcgct tcgcgcgcgt gacgccgtcg gagttcgcgt cgctgaccga cgaggtgctc
    10441 tctcccgcgc ccgacgcgcc tcgcgagctg ccgacgaagg tgctgtacgc gtcttcgact
    10501 ttgtagctcc aacggtctga cgaccgcgcg aaccttcgct gacttttcct gaccggtgat
    10561 tttgaaatga tgaaaggact gatagatatg agcgatgttg atgtattcgt gatcgatgac
    10621 gatttcgatc ccctggccga cctgagcctg gacgacggcg agcgggacga tgccgaggcg
    10681 gattacctgc cgcccatccc cgatgccgac aagagcgtgg tgcctcccgt ggtgccgttg
    10741 tctgcgaccg agcgcatcga gaagctgctg gcgggcattc ccggccagca gttccgcctg
    10801 ctgcacgccg tggaattctg caccgagccg aagacgatgg acgaggccgt ggccaatttg
    10861 gacgcggcgt atccgaacac gacgagcgtg tacggctcgg cgcaggtggt gcagctgctc
    10921 gagcgcgacg gcgcgctgga gcgcatcgtg gacgaggacg ccgcccatgc ggccgccggc
    10981 gacgctgccg atacgcccgc cgaggacgag ggcttcatct cggtgacgcc cgctccgccg
    11041 tgtcactacc gtgccacgca agcgggcctc gatgccgtcg cggcgcacgt caacgagggc
    11101 ctcgtggcgg agcggatcag cgaagacgag cgctatctgc ccatcttcca gcgcgtgctt
    11161 gagatgtgcg cgcgcgaagg aggctgcccc accaaggagc tggaccaggc ggtggacggc
    11221 gacccgctgt gccaagagcc gcgccgcttc tgcgggttct tccgcggcaa gctggaagaa
    11281 acaggcgcca tcgaatggcg cgacgcctgg accattaccg atctggggcg cagcgtgctc
    11341 gcgtccggct tgttcgccgc cgcttccccg tccgaacgct agagaaggag ccacccatgt
    11401 cccacgatca ggaaaccacc atcgccgacg tgctgcgcgg ccgcatggca acctaccagt
    11461 tcctctcgcg cctgttccgc gtggaggtgg accaggagct gtacgacacg ctgatgtcca
    11521 tgcgcttccc ggcgaacacc ggcaacgcgc tggtggacga aggctaccgg atgatctgcg
    11581 gttatctgag ccaagccgac ggcacggtgc tcaccgagct ggcggtggac tacgtgcgcg
    11641 cgttcatcgg gtcgggcaac gacggcttct cggccgcgta cccgtacgag tcggtgtaca
    11701 cgagcccgaa gcgcctcatg atgcaggacg cccgcgacga ggtgctggtg ctgtaccgcg
    11761 ccttcggtct ggacaagcag gaaagctgga aggagggcga ggaccatatc gcgctggagc
    11821 tggagttcga gcagatccta tgcgagcgcg ctatcagggc gtacgaggcc ggcgacgagg
    11881 acgagtgcct gaagctgctg ctgtcgcagc gcaacttcct ggaggaccac ctgctggcgt
    11941 ggtaccccat gatggcggcc gacttgcaga agttcccgca gaccgacttc tacaagggcc
    12001 tgggcaagct gaccgacggc ttcctgcgca acgaccgcga atttttggac gccgtgctgt
    12061 ctgagaacga agcggattgc catccagagc ggaggccgca ggccgaagcc gaaggatccc
    12121 gtgcggcgtc ggccgaaacc gaagtcgagg ttgcatgatg gacgagcacg gcatcgagcc
    12181 caagcgcgat acgggcccct gcgtgtcgcg ccgcacgttc gccctgggag ccgtgggagc
    12241 gtgcgccgtc atcgggctgg gcggcgtgaa gtacctgccc agtgccacgt tgctgcgccc
    12301 gccgggcggg caggacgagg agcagctggc gcgcggctgc ctgcattgcg agaagtgccg
    12361 cgaggtgtgc ccgaagcatg ccatcgctcc tgctcatctt gaggacggca tcttgaacgc
    12421 gcgcacaccg cgcatggact tcaaaagcgg ctggtgcgat ttctgcgaga acgagccggg
    12481 cgggccgaag tgcgtcgcgg cgtgcccgac gcatgcgctt tcgtgccccg acccgtcgca
    12541 ggcgatcatc ggcaaggcgg agatcaaccg cgactggtgc cttgccgcga agggcatggg
    12601 ctgccacgag tgcgtcgacg tgtgcaacta cgaggcgctc gagctgggcg ccgacaacgt
    12661 gccggtggtg gacgtggatg cgtgcaacgg ctgcggcgcc tgcgagctgg cgtgcatctc
    12721 gctgtcggca ggttccatca cggccggcgc caccgatcgc gccatcgtcg tccgtccgac
    12781 cgaagaagtg gaggcgtgat caggatgtcc ctgatcaacg gcctgctggc cctgttgggc
    12841 gagatcgaga gcgatgccat cgccgtgcat gaggaacgct gcatctcggt tcgcaaccgc
    12901 aacgccgact gcctgcgctg cgtggaggca tgcacgtcgg gcgcgctggc gtaccgcgcg
    12961 ggcgagctgc tcgtagagcc cgagcgctgc atcggatgcg gcacgtgcgc cacggcgtgc
    13021 cccacctgcg ccatcgagct gcgcaacccg acggatgccg agctgacggc gcagctcaag
    13081 cactccatcg tggcgacgaa aggccatccg gtcatcgtgt gcgagcaggc gctcgaggcg
    13141 gccaacgtcg cggccgacga tgcgtcggcc gcgtgcgcgg tgccgtgcct ggggcgcatg
    13201 gacgagtcgg cgctcgtggg gttggcggcg taccgcgcct tcgacgcgac gctggcctgc
    13261 ggttcgtgcg agacgtgccc acatgctccg ggcggcgcgc tcgtgcgcga ggtggtggag
    13321 ggcgcgcgca acctgcttga ggcgttcgga tcctccatgc ccatcgacct ggaggaatgc
    13381 gtgcccgagc gcgtgttgga gcccggcggg tcgcacggcc aggcaggcag cgacggggtg
    13441 gggcggcgcg acttcttccg ctcggccaag gacgcatcga cccgcgcagc tggcgcggcg
    13501 gtggccgagg agttggggat ggtcgaggcc gagcccgttc ctgcggcgca tcgcaaagtg
    13561 ggcgcagatg ggacgctgtc gcagttcgtc cccacgcgcc gcgtgcggct gtacaactac
    13621 ctgcagcacg tggggcaacc gacggccgac gaggtggaga cgcgtgtcat cggcgccgtg
    13681 tccatcgatg cccagaagtg cagttcgtgc cgcatgtgcg cggtgttctg tcccacgggc
    13741 gccatcaaga agctcgacga gggcggcgtg ttcggcatcg tgcaccgtcc gagcgcctgc
    13801 atgcagtgcc gcctgtgcga gcacatctgt cccgagcagg ccatcgcggt gagcgggcgc
    13861 gtgcccatca agcagttcat gggcaaggaa gccgtgtgct tcgctatgaa gcgtccaacc
    13921 tggaccccga acaagccctc atcgatgtac gataagctgc acgcgacgct cggcgaacac
    13981 ttggagatgt gcatgttcta gactagtcga ctcacactgg ctcaccttcg ggtgggcctt
    14041 tctgcgttta tatactagag agagaatata aaaagccaga ttattaatcc ggctttttta
    14101 ttatttaatt cctgttataa aaaaaggatc aattttgaac tctctcccaa agttgatccc
    14161 ttaacgatcc ctgcaggaga aggccatcct gacggatggc ctttttgaag ctttctagaa
    14221 tcgggggccc ccggggggat tctagaacaa tggaagtcct cgtctcgccc tcgaattagc
    14281 ccgcttaatg agcgggcttt ttttgaatta attctcgcga gctggcacga caggtttccc
    14341 gactggaaag cgggcagtga gcgcaacgca attaatgtaa gttagcgcga attgcaagct
    14401 ggccgacgcg ctgggctacg tcttgctggc gttcgggagc agaagagcat acatctggaa
    14461 gcaaagccag gaaagcggcc tatggagctg tgcggcagcg ctcagtaggc aatttttcaa
    14521 aatattgtta agccttttct gagcatggta tttttcatgg tattaccaat tagcaggaaa
    14581 ataagccatt gaatataaaa gataaaaatg tcttgtttac aatagagtgg ggggggtcag
    14641 cctgccgcct tgggccgggt gatgtcgtac ttgcccgccg cgaactcggt taccgtccag
    14701 cccagcgcga ccagctccgg caacgcctcg cgcacccgct ggcggcgctt gcgcatggtc
    14761 gaaccactgg cctctgacgg ccagacatag ccgcacaagg tatctatgga agccttgccg
    14821 gttttgccgg ggtcgatcca gccacacagc cgctggtgca gcaggcgggc ggtttcgctg
    14881 tccagcgccc gcacctcgtc catgctgatg cgcacatgct ggccgccacc catgacggcc
    14941 tgcgcgatca aggggttcag ggccacgtac aggcgcccgt ccgcctcgtc gctggcgtac
    15001 tccgacagca gccgaaaccc ctgccgcttg cggccattct gggcgatgat ggataccttc
    15061 caaaggcgct cgatgcagtc ctgtatgtgc ttgagcgccc caccactatc gacctctgcc
    15121 ccgatttcct ttgccagcgc ccgatagcta cctttgacca ccatggcatc agcggtgacg
    15181 gcctcccact tgggttccag gaacagccgg agctgccgtc cgccttcggt cttgggttcc
    15241 gggccaagca ctaggccatt aggcccagcc atggccacca gcccttgcag gatgcgcaga
    15301 tcatcagcgc ccagcggctc cgggccgctg aactcgatcc gcttgccgtc gccgtagtca
    15361 tacgtcacgt ccagcttgct gcgcttgcgc tcgccccgct tgagggcacg gaacaggccg
    15421 ggggccagac agtgcgccgg gtcgtgtcgg acgtggctga ggctgtgctt gttcttaggc
    15481 ttcaccacgg ggcaccccct tgctcttgcg ctgcctctcc agcacggcgg gcttgagcac
    15541 cccgccgtca tgccgcctga accaccgatc agcgaacggt gcgccatagt tggccttgct
    15601 cacaccgaag cggacgaaga accggcgctg gtcgtcgtcc acaccccatt cctcggcctc
    15661 ggcgctggtc atgctcgaca ggtaggactg ccagcggatg ttatcgacca gtaccgagct
    15721 gccccggctg gcctgctgct ggtcgcctgc gcccatcatg gccgcgccct tgctggcatg
    15781 gtgcaggaac acgatagagc acccggtatc ggcggcgatg gcctccatgc gaccgatgac
    15841 ctgggccatg gggccgctgg cgttttcttc ctcgatgtgg aaccggcgca gcgtgtccag
    15901 caccatcagg cggcggccct cggcggcgcg cttgaggccg tcgaaccact ccggggccat
    15961 gatgttgggc aggctgccga tcagcggctg gatcagcagg ccgtcagcca cggcttgccg
    16021 ttcctcggcg ctgaggtgcg ccccaagggc gtgcaggcgg tgatgaatgg cggtgggcgg
    16081 gtcttcggcg ggcaggtaga tcaccgggcc ggtgggcagt tcgcccacct ccagcagatc
    16141 cggcccgcct gcaatctgtg cggccagttg cagggccagc atggatttac cggcaccacc
    16201 gggcgacacc agcgccccga ccgtaccggc caccatgttg ggcaaaacgt agtccagcgg
    16261 tggcggcgct gctgcgaacg cctccagaat attgataggc ttatgggtag ccattgattg
    16321 cctcctttgc aggcagttgg tggttaggcg ctggcggggt cactaccccc gccctgcgcc
    16381 gctctgagtt cttccaggca ctcgcgcagc gcctcgtatt cgtcgtcggt cagccagaac
    16441 ttgcgctgac gcatcccttt ggccttcatg cgctcggcat atcgcgcttg gcgtacagcg
    16501 tcagggctgg ccagcaggtc gccggtctgc ttgtcctttt ggtctttcat atcagtcacc
    16561 gagaaacttg ccggggccga aaggcttgtc ttcgcggaac aaggacaagg tgcagccgtc
    16621 aaggttaagg ctggccatat cagcgactga aaagcggcca gcctcggcct tgtttgacgt
    16681 ataaccaaag ccaccgggca accaatagcc cttgtcactt ttgatcaggt agaccgaccc
    16741 tgaagcgctt ttttcgtatt ccataaaacc cccttctgtg cgtgagtact catagtataa
    16801 caggcgtgag taccaacgca agcactacat gctgaaatct ggcccgcccc tgtccatgcc
    16861 tcgctggcgg ggtgccggtg cccgtgccag ctcggcccgc gcaagctgga cgctgggcag
    16921 acccatgacc ttgctgacgg tgcgctcgat gtaatccgct tcgtggccgg gcttgcgctc
    16981 tgccagcgct gggctggcct cggccatggc cttgccgatt tcctcggcac tgcggccccg
    17041 gctggccagc ttctgcgcgg cgataaagtc gcacttgctg aggtcatcac cgaagcgctt
    17101 gaccagcccg gccatctcgc tgcggtactc gtccagcgcc gtgcgccggt ggcggctaag
    17161 ctgccgctcg gacagttcga ggctggccag cctgcgggcc ttctcctgct gccgctgggc
    17221 ctgctcgatc tgctggccag cctgctgcac cagcgccggg ccagcggtgg cggtcttgcc
    17281 cttggattca cgcagcagca cccacggctg ataaccggcg cgggtggtgt gcttgtcctt
    17341 gcggttggtg aagcccgcca agcggccata gtggcggctg tcggcgctgg ccgggtcggc
    17401 gtcgtactcg ctggccagcg tccgggcaat ctgcccccga agttcaccgc ctgcggcgtc
    17461 ggccaccttg acccatgcct gatagttctt cgggctggtt tccactacca gggcaggctc
    17521 ccggccctcg gctttcatgt catccaggtc aaactcgctg aggtcgtcca ccagcaccag
    17581 accatgccgc tcctgctcgg cgggcctgat atacacgtca ttgccctggg cattcatccg
    17641 cttgagccat ggcgtgttct ggagcacttc ggcggctgac cattcccggt tcatcatctg
    17701 gccggtggtg gcgtccctga cgccgatatc gaagcgctca cagcccatgg ccttgagctg
    17761 tcggcctatg gcctgcaaag tcctgtcgtt cttcatcggg ccaccaagcg cagccagatc
    17821 gagccgtcct cggttgtcag tggcgtcagg tcgagcaaga gcaacgatgc gatcagcagc
    17881 accaccgtag gcatcatgga agccagcatc acggttagcc atagcttcca gtgccacccc
    17941 cgcgacgcgc tccgggcgct ctgcgcggcg ctgctcacct cggcggctac ctcccgcaac
    18001 tctttggcca gctccaccca tgccgcccct gtctggcgct gggctttcag ccactccgcc
    18061 gcctgcgcct cgctggcctg ctgggtctgg ctcatgacct gccgggcttc gtcggccagt
    18121 gtcgccatgc tctgggccag cggttcgatc tgctccgcta actcgttgat gcctctggat
    18181 ttcttcactc tgtcgattgc gttcatggtc tattgcctcc cggtattcct gtaagtcgat
    18241 gatctgggcg ttggcggtgt cgatgttcag ggccacgtct gcccggtcgg tgcggatgcc
    18301 ccggccttcc atctccacca cgttcggccc caggtgaaca ccgggcaggc gctcgatgcc
    18361 ctgcgcctca agtgttctgt ggtcaatgcg ggcgtcgtgg cctgcccgct ctaatgcccg
    18421 gttggcatgg tcggcccatg cctcgcgggt ctgctcaagc catgccttgg gcttgagcgc
    18481 ttcggtcttc tgtgccccgc ccttctccgg ggtcttgccg ttgtaccgct tgaaccactg
    18541 agcggcgggc cgctcgatgc cgtcattgat ccgctcggag atcatcaggt ggcagtgcgg
    18601 gttctcgccg ccaccggcat ggatggccag cgtatacggc aggcgctcgg caccggtcag
    18661 gtgctgggcg aactcggacg ccagcgcctt ctgctggtcg agggtcagct cgaccggcag
    18721 ggcaaattcg acctccttga acagccgccc attggcgcgt tcatacaggt cggcagcatc
    18781 ccagtagtcg gcgggccgct cgacgaactc cggcatgtgc ccggattcgg cgtgcaagac
    18841 ttcatccatg tcgcgggcat acttgccttc gcgctggatg aagtcggcct tggccctggc
    18901 cgattggccg cccgacctgc tgccggtttt cgccgtaagg tgataaatcg ccatgctgcc
    18961 tcgctgttgc ttttgctttt cggctccatg caatggccct cggagagcgc accgcccgaa
    19021 gggtggccgt taggccagtt tctcgaagag aaaccggtaa gtgcgccctc ccctacaaag
    19081 tagggtcggg attgccgccg ctgtgcctcc atgatagcct acgagacagc acattaacaa
    19141 tggggtgtca agatggttaa ggggagcaac aaggcggcgg atcggctggc caagctcgaa
    19201 gaacaacgag cgcgaatcaa tgccgaaatt cagcgggtgc gggcaaggga acagcagcaa
    19261 gagcgcaaga acgaaacaag gcgcaaggtg ctggtggggg ccatgatttt ggccaaggtg
    19321 aacagcagcg agtggccgga ggatcggctc atggcggcaa tggatgcgta ccttgaacgc
    19381 gaccacgacc gcgccttgtt cggtctgccg ccacgccaga aggatgagcc gggctgaatg
    19441 atcgaccgag acaggccctg cggggctgca cacgcgcccc cacccttcgg gtagggggaa
    19501 aggccgctaa agcggctaaa agcgctccag cgtatttctg cggggtttgg tgtggggttt
    19561 agcgggcttt gcccgccttt ccccctgccg cgcagcggtg gggcggtgtg tagcctagcg
    19621 cagcgaatag accagctatc cggcctctgg ccgggcatat tgggcaaggg cagcagcgcc
    19681 ccacaagggc gctgataacc gcgcctagtg gattattctt agataatcat ggatggattt
    19741 ttccaacacc ccgccagccc ccgcccctgc tgggtttgca ggtttggggg cgtgacagtt
    19801 attgcagggg ttcgtgacag ttattgcagg ggggcgtgac agttattgca ggggttcgtg
    19861 acagttagta cgggagtgac gggcactggc tggcaatgtc tagcaacggc aggcatttcg
    19921 gctgagggta aaagaacttt ccgctaagcg atagactgta tgtaaacaca gtattgcaag
    19981 gacgcggaac atgcctcatg tggcggccct cgcgagaatt aattcagata aaaaaaatcc
    20041 ttagctttcg ctaaggatga tttctagcga tgaccctgct gattggttcg ctgaccgtag
    20101 actggttggc ttggtttcat cagccatccg cttgccctca tctgttacgc cggcggtagc
    20161 cggccagcct cgcagagcag gattcccgtt gagcaccgcc aggtgcgaat aagggacagt
    20221 gaagaaggaa cacccgctcg cgggtgggcc tacttcacct atcctgcccg gctgacgccg
    20281 ttggatacac caaggaaagt ctacacgaac cctttggcaa aatcctgtat atcgtgcgac
    20341 atcgcgcgcg cgcgcgcgga tgctagcggc gcgccaaaaa aaagcccgct catta
//
